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MVP17 encodes a mitochondrial inner-membrane protein, and mutation of human MVP17 can cause mitochondria DNA depletion syndrome (MDDS). However, the underlying function of mpv17 is still elusive. Here, we developed a new mutant with mpv17 knockout by using the CRISPR/Cas9 system. The mpv17−/− zebrafish showed developmental defects in muscles, liver, and energy supply. The mpv17−/− larvae hardly survived beyond a month, and they showed abnormal growth during the development stage. Abnormal swimming ability was also found in the mpv17−/− zebrafish. The transmission electron microscope (TEM) observation indicated that the mpv17−/− zebrafish underwent severe mitochondria dysfunction and the disorder of mitochondrial cristae. As an energy producer, the defects of mitochondria significantly reduced ATP content in mpv17−/− zebrafish, compared to wild-type zebrafish. We hypothesized that the disorder of mitochondria cristae was contributed to the dysfunction of muscle and liver in the mpv17−/− zebrafish. Moreover, the content of major energy depot triglycerides (TAG) was decreased dramatically. Interestingly, after rescued with normal exogenous mitochondria by microinjection, the genes involved in the TAG metabolism pathway were recovered to a normal level. Taken together, this is the first report of developmental defects in muscles, liver, and energy supply via mitochondria dysfunction, and reveals the functional mechanism of mpv17 in zebrafish.

Located deep in the Kelabit Highlands in Malaysian Borneo, the remote town of Bario offers us a natural laboratory of rural electrification projects through which to understand end-user perceptions of success and failure, and the factors that contribute to these perceptions. We use a case-study based approach and focus on three off-grid energy projects: a 110 kW mini-hydro power plant; a 12 kW wind turbine system; and a 1.59 MW solar-diesel hybrid system. We find that end-users primarily see the success or failure of a project in technical terms, but that this narrow conceptualization masks important interactions between technical, economic and institutional factors. We further find that end-user perceptions of newer projects are heavily affected by their perceptions of previous projects. Our findings suggest several ways forward to improve the effectiveness of rural electrification initiatives. Firstly, we should expect complex interactions between technical, economic and institutional factors and build-in the necessary capacity into rural electrification projects to identify and address these interactions. Secondly, there is no one size fits all solution to creating the requisite capacity to deal with interactions among technical, economic and institutional factors. Thirdly, ongoing qualitative or mixed-methods evaluations of rural electrification initiatives can help unravel what would otherwise be hidden interactions between technical, economic and institutional factors.

The evolution of insect resistance to pesticides poses a continuing threat to agriculture and human health. While much is known about the proximate molecular and biochemical mechanisms that confer resistance, far less is known about the regulation of the specific genes/gene families involved, particularly by trans-acting factors such as signal-regulated transcription factors. Here we resolve in fine detail the trans-regulation of CYP6CM1, a cytochrome P450 that confers resistance to neonicotinoid insecticides in the whitefly Bemisia tabaci, by the mitogen-activated protein kinase (MAPK)-directed activation of the transcription factor cAMP-response element binding protein (CREB). Reporter gene assays were used to identify the putative promoter of CYP6CM1, but no consistent polymorphisms were observed in the promoter of a resistant strain of B. tabaci (imidacloprid-resistant, IMR), which overexpresses this gene, compared to a susceptible strain (imidacloprid-susceptible, IMS). Investigation of potential trans-acting factors using in vitro and in vivo assays demonstrated that the bZIP transcription factor CREB directly regulates CYP6CM1 expression by binding to a cAMP-response element (CRE)-like site in the promoter of this gene. CREB is overexpressed in the IMR strain, and inhibitor, luciferase, and RNA interference assays revealed that a signaling pathway of MAPKs mediates the activation of CREB, and thus the increased expression of CYP6CM1, by phosphorylation-mediated signal transduction. Collectively, these results provide mechanistic insights into the regulation of xenobiotic responses in insects and implicate both the MAPK-signaling pathway and a transcription factor in the development of pesticide resistance.

GQDs show great potential in drug carriers, bioimaging, biosensors, theranostics, and are recently reported as promising therapeutic agents to treat amyloid-related diseases such as Parkinson's disease and inflammations such as colitis. However, current toxicity data about GQDs based on in vivo toxicity assessments remain scarce. In the study, we examined the mRNA expression changes of zebrafish embryos exposed to four types of GQDs, including raw graphene quantum dots (R-GQDs), graphene oxide quantum dots (GOQDs), carboxyl GQDs (C-GQDs), and aminated GQDs (A-GQDs). Firstly, we treated embryos with the four GQDs at three concentrations (50, 100, and 200 μg/mL), and found that only A-GQDs caused embryonic developmental arrest at 100 and 200 μg/mL with significantly decreased survival rates and heartbeat rates, as well as the elevated malformation rates. Next, we analyzed the mRNA sequencing data acquired from zebrafish embryos exposed to the four GQDs for 7 days at 100 μg/mL, and found that all GQDs can act on potassium (K

Background To explore the application value of free omental wrapping and modified pancreaticojejunostomy in pancreaticoduodenectomy (PD). Methods The clinical data of 175 patients who underwent pancreaticoduodenectomy from January 2015 to December 2020 were retrospectively analysed. In total, 86 cases were divided into Group A (omental wrapping and modified pancreaticojejunostomy) and 89 cases were divided into Group B (control group). The incidences of postoperative pancreatic fistula and other complications were compared between the two groups, and univariate and multivariate logistic regression analyses were used to determine the potential risk factors for postoperative pancreatic fistula. Risk factors associated with postoperative overall survival were identified using Cox regression. Results The incidences of grade B/C pancreatic fistula, bile leakage, delayed bleeding, and reoperation in Group A were lower than those in Group B, and the differences were statistically significant (P Conclusions Wrapping and isolating the modified pancreaticojejunostomy with free greater omentum can significantly reduce the incidence of postoperative pancreatic fistula and related complications, inhibit the development of inflammation, and favourably affect prognosis.

The evolution of insect resistance to pesticides poses a continuing threat to agriculture and human health. While much is known about the proximate molecular and biochemical mechanisms that confer resistance, far less is known about the regulation of the specific genes/gene families involved, particularly by trans-acting factors such as signal-regulated transcription factors. Here we resolve in fine detail the trans-regulation of CYP6CM1, a cytochrome P450 that confers resistance to neonicotinoid insecticides in the whitefly Bemisia tabaci, by the mitogen-activated protein kinase (MAPK)-directed activation of the transcription factor cAMP-response element binding protein (CREB). Reporter gene assays were used to identify the putative promoter of CYP6CM1, but no consistent polymorphisms were observed in the promoter of a resistant strain of B. tabaci (imidacloprid-resistant, IMR), which overexpresses this gene, compared to a susceptible strain (imidacloprid-susceptible, IMS). Investigation of potential trans-acting factors using in vitro and in vivo assays demonstrated that the bZIP transcription factor CREB directly regulates CYP6CM1 expression by binding to a cAMP-response element (CRE)-like site in the promoter of this gene. CREB is overexpressed in the IMR strain, and inhibitor, luciferase, and RNA interference assays revealed that a signaling pathway of MAPKs mediates the activation of CREB, and thus the increased expression of CYP6CM1, by phosphorylation-mediated signal transduction. Collectively, these results provide mechanistic insights into the regulation of xenobiotic responses in insects and implicate both the MAPK-signaling pathway and a transcription factor in the development of pesticide resistance.

Currently, microplastics (MPs) attracted increased attention for their ubiquity and toxic properties. In this study, marine medaka was used to explore the individual and combined toxicity of heavy metals (HMs) and MPs on intestinal bacteria and gonadal development. After exposure to environmentally relevant concentrations of MPs and HMs, significant alterations of intestinal microbiota were found. The MPs treatment reduced the diversity and abundance of intestinal microbiota, while the HMs and MPs-HMs treatments increased them, which were confirmed by the abundance changes of Proteobacteria. According to the KEGG analysis, the metabolism and environmental information processing (EIP) pathways in the microbial community were significantly affected. This study showed that the MPs-HMs treatment caused a higher pollution load on the gut of the marine medaka, and triggered more significant changes of specific bacterial species and gut function in the males. However, during the gonadal development, HMs and MPs-HMs treatments resulted in empty follicles (EF) and follicular atresia (FA), and altered the gene expression levels related to hypothalamic-pituitary-gonadal (HPG) axis. In short, this study demonstrated that the reproductive disturbance was mainly due to HMs, but the combination of MPs and HMs did not strengthen the risk to the gonad development of the marine medaka.

Graphene quantum dots (GQDs) are widely used for biomedical applications. Previously, the low-level toxicity of GQDs in vivo and in vitro has been elucidated, but the underlying molecular mechanisms remained largely unknown. Here, we employed the Illumina high-throughput RNA-sequencing to explore the whole-transcriptome profiling of zebrafish larvae after exposure to GQDs. Comparative transcriptome analysis identified 2116 differentially expressed genes between GQDs exposed groups and control. Functional classification demonstrated that a large proportion of genes involved in acute inflammatory responses and detoxifying process were significantly up-regulated by GQDs. The inferred gene regulatory network suggested that activator protein 1 (AP-1) was the early-response transcription factor in the linkage of a cascade of downstream (pro-) inflammatory signals with the apoptosis signals. Moreover, hierarchical signaling threshold determined the high sensitivity of complement system in zebrafish when exposed to the sublethal dose of GQDs. Further, 35 candidate genes from various signaling pathways were further validated by qPCR after exposure to 25, 50, and 100 μg/mL of GQDs. Taken together, our study provided a valuable insight into the molecular mechanisms of potential bleeding risks and detoxifying processes in response to GQDs exposure, thereby establishing a mechanistic basis for the biosafety evaluation of GQDs.

The impounding level of 175m for the Three Gorges Reservoir (TGR) is of vital importance for efficient flood control, power generation and convenient navigation in China. However, little is known about the spatial distribution and toxicological risks of major pollutants in the Three Gorges Reservoir Area (TGRA) at that stage. The aim of this study is to probe the ubiquitous polycyclic aromatic hydrocarbons (PAHs) contamination and toxicological impacts in the surface water of the TGRA at the highest water impoundment level of 175m. Our results showed that the ƩPAHs levels ranged from 83 to 1631ng/L in the upper reaches, 354 to 1159ng/L in the middle reaches, and 23 to 747ng/L in the lower reaches of the TGRA. Source apportionment of PAHs indicated that coal combustion, industrial emissions, heavy traffic, agriculture and shipping activities were the primary sources. Compositional pattern highlighted >85% dominancy of low molecular weight (LMW) PAHs in the reservoir. Risk assessment based on risk quotients (RQs) implied moderate to high ecological risks: the upper reaches>the middle reaches>the lower reaches. However, gene expression profiles portrayed contrary scenario because of the presence of relatively higher footprints of high molecular weight (HMW) PAHs in the middle and the lower reaches, which was confirmed by Cox hazard proportional model. Moreover, the transgenic zebrafish Tg(cyp1a:gfp) induced by PAHs also expressed stronger fluorescent signals in the middle and lower reaches. Taken together, different approaches were employed to firstly reveal the real status of ecological toxicity of PAHs and explore the underlying mechanisms at the highest impounding level of 175m in the TGRA.

The balance between anti- and pro-oxidant activities is of great important to maintain the biochemical and physiological homeostasis in the human body. Developing new therapeutic strategies to reduce health risks caused by free radicals has always been research focus over the past decades. Szechuan pepper, a characteristic pungent-flavored spice in Sichuanese cuisine, recently attracts the attention of researchers for its widespread therapeutic effects on acute and chronic diseases. The plant produces the innocuous 'tingling and numbing' sensations across the oral cavity by stirring specific neuron types, which are mechanically distinct from those excited by capsaicin. Furthermore, the extracts or the compounds of Szechuan pepper are biochemically proven to possess strong antioxidant activities that could scavenge free radicals and inhibit overactive peroxidase system in pathological models. Herein, the review emphasizes the molecular basis underlying the neurophysiological and antioxidant activities of the plant by a comprehensive analysis of various signaling pathways in disease models treated by Szechuan pepper. Further, we performed a broadening analysis to unearth potential signaling pathways associated with the antioxidant roles of the plant.

Pharmaceuticals and personal care products (PPCPs), the pollutants of emerging concerns, present potential risks to the ecological environment. This study focused on the prioritization of widely used selected PPCPs belonging to two categories:personal care products (PCPs) and non-steroidal anti-inflammatory drugs (NSAIDs). We predicted the toxicogenetic endpoints of PPCPs and then confirmed them using experimental approaches. Our results revealed a significant similarity in the findings obtained through both approaches, indicating NSAIDs with relatively high environmental impacts and in vitro/vivo toxicity. Experimental approach revealed that musk xylene (MX) from PCPs and DIC from NSAIDs as individual chemicals of priority concern showed elevated environmental impacts and significantly induced pi3k-akt-mTOR in vitro. Similarly, propyl paraben (PP) from PCPs and diclofenac (DIC) from NSAIDs caused significant cytotoxicity and DNA damage in vitro. Moreover, PP and MX from the PCPs group and naproxen (NAP) and DIC from the NSAIDs group induced developmental toxicity and perturbations to phases I, II, and III detoxification pathways in vivo. In addition, MX and DIC as priority PPCPs inhibited hematopoiesis and hepatogenesis in vivo. Apart from the specific effects, PPCPs can be ranked as: MX > PP > methylparaben (MP) for PCPs, and DIC > NAP > ibuprofen (IBU) for NSAIDs, regarding their toxic and environmental concerns.

The outlet flow field of an annular jet for a bladeless fan was investigated experimentally using CTA (Constant Temperature Anemometer) hot-wire system in detail, at five Reynolds numbers ranging from 28,200 to 40,100. From the experiment results, it can be concluded that: (1) the time-averaged velocity in both the horizontal and vertical radial directions, and the time-averaged velocity and turbulence intensity on the axis, all increase with increasing Reynolds number; (2) some variation situations of Sk (skewness) and Ku (kurtosis) of PDF (Probability Density Function) for dimensionless fluctuating velocity on the axis inversely dependent on Reynolds number, indicating that the decrease of Reynolds number intensifies the motion of large-scale coherent structures; (3) the annular jet within the outlet flow field starts to converge about at the cross-section of 1.5d (d represents the throat diameter of the Coanda surface) at each Reynolds number; the lower part of the flow field is combined upwards into the upper part; this combination has been finished about at cross-section of 3d; (4) the combined jet is similar to a classical single jet, the peak decay slope increasing with increasing Reynolds number; also, the intrinsic flow mechanism of this flow field structure has been revealed.

Take water hydraulic slide valve core as the research object. Laser surface texturing (LST) technology was used to improve the valve core carrying capacity and hydrodynamic lubrication characteristics, which can process micro-texture on the surface of the core. And CFD method was taken to calculate flow field of smooth and laser surface texturing core respectively. For studying the influence of valve core micro-texture and valve core overlap lengths on hydrodynamic lubrication characteristic and carrying capacity of the core, core surface pressure distribution, surface pressure and friction force curves were drawn. Results show: The smooth valve core does not produce hydrodynamic lubrication effect, but textured valve core can bring the oil film load capacity, so as to make the effect of mixed friction between valve core and sleeve weaken. And along with the decrease of the core overlap length, the carrying capacity on the surface of the textured valve core does not decrease but increase when the valve core overlap length amount is greater than 2.5mm.The results provide important theoretical reference of studying and applying LST process technology to the valve core friction pair’s lubrication performance.

This research is focused on dynamic performance of water hydraulic single hydraulic prop, the mathematical and AMESim model of single hydraulic prop are established. And the drop hammer is introduced to simulate the impact load of the surrounding rock acting on the prop. The performance parameters of prop retract displacement and cavity pressure are used as the research objects. The working process of single hydraulic prop is reproduced by the simulation. And the influence of safety valve parameters on the support system is analyzed. The results show that: increasing maximal valve core stroke and dead volume or reducing valve damping hole diameter can improve the support performance of the single hydraulic prop. But the influence of equivalent damping has two sides. For support system, decrease damping can improve the support performance, but for the safety valve, reduce the damping make the valve core stability decline. In addition, the pressure overshoot of the optimized system is 18.3%, adjusting time is 0.5s and the retract displacement is 10mm. The dynamic performances meet the technology requirements of the coal mine safety production of China.

The outlet flow field of the air multiplier was simulated by a RNG k – e model based on compressible fluid, and the simulated results were validated experimentally through a Constant Temperature Anemometer (CTA) hot-wire system and the ventilating multi-parameter testing instrument. The flow characteristics and the reason for the increase of the output flow rate relative to the turbine intake rate were revealed by investigating profiles of the time-averaged velocity and pressure. The results indicate that (1) the flow parameter profiles in the outlet flow field are not uniform, and the entire time-averaged velocity field and pressure field in near field are basically axis symmetrical in the horizontal direction instead of in the vertical direction; (2) the entrainment and suction effects resulted from the “coronary zone” of the established flow of high intense turbulence jet, enabling the air multiplier to realize “air multiplication”, and the time-averaged velocity of the upper “coronary zone” and pressures in near field make greater contributions to the increase of flow rate. (3) RNG k – e model is reliable on predicting the time-averaged flow behavior in the outlet flow field for the air multiplier.

Graphene quantum dots (GQDs) are well-known for its potential applications for bioimaging, biosensor, and drug carrier in biomedicine. GQDs are well characteristic of intrinsic peroxidase-like catalytic activity, which is proven effective in scavenging the free radicals, such assuperoxide anion, hydrogen peroxide, and hydroxyl radical. GQDs are also well praised for its low in vivo and in vitro toxicity. Here, we found that nitrogen-doped GQDs (N-GQDs) can strongly disturb redox-sensitive system via the selective inhibition of endogenous antioxidant enzyme activities in zebrafish. The enzyme activities or transcription levels of a battery of hemoproteins including catalase (CAT), superoxide dismutase (SOD), respiratory chain complex I, complex Ⅲ, hemoglobin (Hb), and myeloperoxidase (MPO), were significantly suppressed by N-GQDs. We also found that N-GQDs activated the cytochrome P450 monooxygenase (e.g. cyp1a) and the associated aryl-hydrocarbon receptor repressors (ahrr1 and ahrr2) in zebrafish embryos. Compared to the ultrasmall graphene oxide (USGO), N-GQDs exhibited stronger fluorescent permeability and tissue-specific bio-accumulative effects. Taken together, our findings highlighted that exposure to N-GQDs can disrupt endogenous antioxidant enzyme activities, possibly via the competitive inhibition of electron transfer process. Our results in this study provided solid data for biosafety evaluations of various types of GQDs, and created an alert for the future biomedical applications of N-GQDs.

Graphene quantum dots (GQDs) has been widely used in enormous fields, however, the inherent molecular mechanism of GQDs for potential risks in biological system is still elusive to date. In this study, the outstanding reduced graphene quantum dots (rGOQDs) with the QY as high as 24.62% were successfully synthesized by the improved Hummers method and DMF hydrothermal treatment approach. The rGOQDs were N-doped photoluminescent nanomaterials with functional groups on the surface. The fluorescent bio-imaging was performed by exposing zebrafish in different concentrations of the as-prepared rGOQDs, and the distribution of rGOQDs was successfully observed. Moreover, the developmental toxicity and genotoxicity were evaluated to further investigate the potential hazard of rGOQDs. The result indicated that rGOQDs were responsible for the dose-dependent abnormalities on the development of zebrafish. Since the real-time polymerase chain reaction (RT-PCR) results showed that the expression of cyp1a was the highest expression in the selected genes and significantly up-regulated 8.49 fold in zebrafish, the perturbation of rGOQDs on aryl hydrocarbon receptor (AhR) pathway was investigated by using the Tg(cyp1a:gfp) zebrafish for the first time. The results demonstrated that rGOQDs significantly increased the green fluorescent protein (GFP) expression promoted by cyp1a in a dose-dependent manner, which was also further confirmed by the western blotting. This study offered an opportunity to reveal the potential hazards of in vivo bio-probes, which provided a valuable reference for investigating the graphene-based materials on the disturbance of AhR pathway in biological organisms.

This paper uses a political ecology approach to unpack the experience of local governments and displaced communities in Sarawak, Malaysia, with Chinese dam construction at the Bakun Hydroelectric Dam. Data for the study was collected over 32 months from 2014 to 2016. The field site offered a unique insight into how recipient countries of aid are also often at the receiving end of domestic politics of donor countries. The paper finds that Chinese and Australian enterprises involved in the dam construction and resettlement of indigenous communities displayed different understandings with regards to social and environmental safeguards, resulting in a dysfunctional handover of the project from Australian to Chinese leadership. Consequently, indigenous communities were dispossessed from their land, affecting their ability to successfully reconstruct their livelihoods, with their attempts to do so causing further damage to the environment around the reservoir of the dam.

Advantages, disadvantages, and drilling adaptability of several rigs earlier used in domestic coal mine are analyzed. CMS1-800/30 crawler jumbo is reconstructed from standing pillar rig according to specific condition of long bedding borehole at coal face which possesses rockburst trend. Wide blade spiral drill pipe and Φ65mm diamond compact bit are used to drill long bedding borehole at headentry and tailentry. Drilling efficiency of long bedding borehole is greatly improved, and effect of relieving stress is very good. Safe and quick mining is ensured, and economic benefit is remarkable.

Juvenile hormone (JH) prevents insect larval metamorphosis and stimulates processes for adult reproduction. Krüppel-homolog 1 (Kr-h1), a zinc finger transcription factor, is shown to mediate the anti-metamorphic effect of JH in both holometabolous and hemimetabolous insects. However, the role of Kr-h1 in JH-mediated reproduction has not been determined. Using the migratory locust, Locusta migratoria, we showed here that Kr-h1 was expressed in response to JH in female adults, and Kr-h1 transcription was directly regulated by the JH-receptor complex comprised of Methoprene-tolerant (Met) and steroid receptor co-activator. We demonstrated that Kr-h1 RNAi phenocopied Met RNAi and JH-deprived condition during post-eclosion development and vitellogenesis of female locusts. Knockdown of Kr-h1 resulted in substantial reduction of Vg expression in the fat body and lipid accumulation in the primary oocytes, accompanied by blocked follicular epithelium development, oocyte maturation and ovarian growth. Our data therefore reveal a crucial role of Kr-h1 in insect vitellogenesis and egg production. This study suggests that JH-Met-Kr-h1 signaling pathway is also functional in insect reproduction.

According to the Eleventh China Intelligent Robot Contest of fire-fighting rules, modified the AS-MF09 fire-fighting robot and had made an overall debug. From the problems arising in the debugging process for the hardware platform based on AS-MF09, this paper made a targeted analysis in the practice, the key technologies such as the strategies of finding the fire, the choice of fire-fighting mode and the efficiency to ensure fire-fighting had been optimized. As the result, the AS-MF09 fire-fighting robot found the fire quickly at the lower scores fire-fighting mode and extinguished the fire with the success rate of one hundred percent. The result shows that the optimization of the AS-MF09 fire-fighting robot can achieve rapid and efficient fire-fighting effects.

Due to special physical and chemical properties of water medium, three-way water hydraulic valve has many key technical problems that could seriouly affect the operating performance. Safety valve in three-way hydraulic valve is seen as a research object, which is aiming at studying the effect of flow field distribution with surface micro modeling in three-way hydraulic valve cavity based on FLUENT. The simulation results show that, proper surface micro modelling help to improve the three-way water hydraulic valve cavity flow field distribution, different structural parameters affecting different flow field distribution, as for micro modelling design, careful consideration of the selection of structure parameters is necessary.

Major recent developments of gear pump with external mesh is introduced in this paper, In order to enlarge the application range of the gear pump, a kind of variable type gear pump is designed. The whole design process is described in detail, the entire structure use a triplet, The driven gear can move through variable stem, it result in the change of displacement, it can refer to design of variable gear pump.

Historically, the greater slow loris Nycticebus coucang has proven to be an elusive animal in Singapore, evading even the prolific British colonial animal collectors. In recent de - cades, sightings of the slow loris have increased remarkably, and these have coincided with an increase in nocturnal surveys. Past trade-record discrepancies, as well as the sighting in the wild of a non-native slow loris species, point to the importance of Singapore for global slow loris con- servation. The first ever ecological survey is documented in this paper, highlighting the difficulty of studying the slow loris in the wild, in habitats hemmed in by urban development, as well as the urgency of further work on Singapore's most poorly studied primate.

The Fukushima disaster was a wake-up call for the nuclear industry as well as a shocking revelation of the inner workings of the Japanese power sector. The political fallout from the event was far-reaching, pushing governments into abandoning nuclear expansion, turning instead to fossil fuels and renewable energy alternatives. While the move away from nuclear energy was deemed a move critical to political survival in Europe, we find that political candidates running on anti-nuclear platforms did not win elections, while the pro-nuclear Liberal Democratic Party won government in the 2012 elections. Against this backdrop, we analyse the energy conflict in Japan using a framework of values versus interests and consider the regulatory and cultural conditions that contributed to the disaster. A number of considerations lie in the way of an organised phase-out of nuclear power in Japan. We also consider the possible policy paths Japan may take.

Local perceptions of climate anomalies influence adaptation behaviour. Specifically, perceptions that are more accurate and homogenous at the community-level are more likely to facilitate the collective action required to adapt to the local effects of climate anomalies experienced by many indigenous communities. We combine primary data on perceptions of climate anomalies from 200 individuals in six Penan villages in Sarawak, Malaysia with instrumental climate data. We find that perceptions of climate anomalies vary substantially in terms of occurrence and magnitude, and do not generally correlate with instrumental climate data. We operationalise the Penan forest sign language (Oroo’) as a measure of traditional ecological knowledge (TEK) and find only weak evidence of a systematic statistical association with perceptions of climate anomalies among our sampled respondents. Our findings suggest caution in advancing adaptation strategies in indigenous communities that are predominantly premised on TEK. Instead, our findings suggest that in designing adaptation measures, indigenous communities may benefit by engaging in forums where community members and external stakeholders can come together, share their perceptions and observations of climate change, and reach a collective consensus on the community-level effects of climate change and pathways towards adaptation.

The absorptions of CO2on the 1-butyl-3-methylimidazolium acetate ([Bmi [Ac]) with different substituents are calculated systematically at GGA/PW91 level. Three hydrogen bonds are formed between [A and cations of 1-n-[Bmi [A ([NBmi+) and 1-tert-[Bmi [A ([TBmi+). The interaction between CO2and the [NBmi [A by a C-O bond is much weaker than that with the [TBmi [A by forming a O...O...C...C four member-ring. The chemisorption of CO2on the ion pairs of [NBmi [A is much weaker than that on the [TBmi [A, resulted from the absorption energies analysis. The frontier molecular orbitals shows the electronic density overlap between absorbed CO2and the [A in CO2-[NBmi [A is much weaker than that in [TBmi [A. Therefore, the chemisorption of CO2on the ion pair of [NBmi [A is much weaker than that on the [TBmi [A. The ionic liquids based [NBmi+can be used repetitively, and the adsorbed CO2would be easier desorbed.