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14. Protein secretion biotechnology using <TOGGLE>Streptomyces lividans</TOGGLE>: Large-scale production of functional trimeric tumor necrosis factor α

Author

Pozidis, Charalambos, Lammertyn, Elke, Politou, Anastasia S., Anné, Jozef, Tsiftsoglou, Asterios S., Sianidis, Giorgos, and Economou, Anastassios

Abstract

We evaluated the feasibility of large-scale production of biopharmaceuticals expressed as heterologous polypeptides from the Gram-positive bacterium Streptomyces lividans. As a model protein we used murine tumor necrosis factor alpha (mTNFα). mTNFα fused C-terminally to the secretory signal peptide of the subtilisin-inhibitor protein from Streptomyces venezuelae. Under appropriate fermentation conditions, significant amounts of mature mTNFα (80–120 mg/L) can be recovered from spent growth media. Efficient downstream processing allowing rapid purification of mTNFα from culture supernatants was developed. Importantly, the protein is recovered from the spent growth medium in its native trimeric state as judged by biophysical analysis. Further, mTNFα secreted by S. lividans is significantly more active in an in vitro apoptosis tissue culture assay than a corresponding polypeptide produced in Escherichia coli. This pilot study provides the first validation of S. lividans protein secretion as an alternative bioprocess for large-scale production of oligomeric proteins of potential therapeutic value. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 72: 611–619, 2001.

Published
2001
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