32 results on '"Sicheng Yan"'
Search Results
2. Advances in retinal imaging biomarkers for the diagnosis of cerebrovascular disease
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Yier Zhang, Ting Zhao, Ling Ye, Sicheng Yan, Wuyue Shentu, Qilun Lai, and Song Qiao
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cerebrovascular disease ,retina ,biomarkers ,cerebral microcirculation ,brain function ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
The increasing incidence and mortality rates of cerebrovascular disease impose a heavy burden on both patients and society. Retinal imaging techniques, such as fundus photography, optical coherence tomography, and optical coherence tomography angiography, can be used for rapid, non-invasive evaluation of cerebral microcirculation and brain function since the retina and the central nervous system share similar embryonic origin characteristics and physiological features. This article aimed to review retinal imaging biomarkers related to cerebrovascular diseases and their applications in cerebrovascular diseases (stroke, cerebral small vessel disease [CSVD], and vascular cognitive impairment [VCI]), thus providing reference for early diagnosis and prevention of cerebrovascular diseases.
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- 2024
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3. Human patient derived organoids: an emerging precision medicine model for gastrointestinal cancer research
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Sicheng Yan, Yuxuan He, Yuehong Zhu, Wangfang Ye, Yan Chen, Cong Zhu, Fuyuan Zhan, and Zhihong Ma
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gastrointestinal cancers ,organoid ,3D model ,CRISPR/cas9 ,personalized medicine ,Biology (General) ,QH301-705.5 - Abstract
Gastrointestinal cancers account for approximately one-third of the total global cancer incidence and mortality with a poor prognosis. It is one of the leading causes of cancer-related deaths worldwide. Most of these diseases lack effective treatment, occurring as a result of inappropriate models to develop safe and potent therapies. As a novel preclinical model, tumor patient-derived organoids (PDOs), can be established from patients’ tumor tissue and cultured in the laboratory in 3D architectures. This 3D model can not only highly simulate and preserve key biological characteristics of the source tumor tissue in vitro but also reproduce the in vivo tumor microenvironment through co-culture. Our review provided an overview of the different in vitro models in current tumor research, the derivation of cells in PDO models, and the application of PDO model technology in gastrointestinal cancers, particularly the applications in combination with CRISPR/Cas9 gene editing technology, tumor microenvironment simulation, drug screening, drug development, and personalized medicine. It also elucidates the ethical status quo of organoid research and the current challenges encountered in clinical research, and offers a forward-looking assessment of the potential paths for clinical organoid research advancement.
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- 2024
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4. Functional abnormalities of the glymphatic system in cognitive disorders
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Wuyue Shentu, Qi Kong, Yier Zhang, Wenyao Li, Qiulu Chen, Sicheng Yan, Junjun Wang, Qilun Lai, Qi Xu, and Song Qiao
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aquaporin-4 ,astrocyte ,cerebrospinal fluid dynamics ,cognitive disorders ,glymphatic system ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Various pathological mechanisms represent distinct therapeutic targets for cognitive disorders, but a balance between clearance and production is essential for maintaining the stability of the brain’s internal environment. Thus, the glymphatic system may represent a common pathway by which to address cognitive disorders. Using the established model of the glymphatic system as our foundation, this review disentangles and analyzes the components of its clearance mechanism, including the initial inflow of cerebrospinal fluid, the mixing of cerebrospinal fluid with interstitial fluid, and the outflow of the mixed fluid and the clearance. Each section summarizes evidence from experimental animal models and human studies, highlighting the normal physiological properties of key structures alongside their pathological manifestations in cognitive disorders. The same pathologic manifestations of different cognitive disorders appearing in the glymphatic system and the same upstream influences are main points of interest of this review. We conclude this article by discussing new findings and outlining the limitations identified in current research progress.
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- 2025
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5. Indirubin Derivative 7-Bromoindirubin-3-Oxime (7Bio) Attenuates Aβ Oligomer-Induced Cognitive Impairments in Mice
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Liping Chen, Chunhui Huang, Jieyi Shentu, Minjun Wang, Sicheng Yan, Fei Zhou, Zaijun Zhang, Chuang Wang, Yifan Han, Qinwen Wang, and Wei Cui
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Alzheimer's disease ,7-bromoindirubin-3-oxime ,β-amyloid ,CDK5 ,GSK3β ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Indirubins are natural occurring alkaloids extracted from indigo dye-containing plants. Indirubins could inhibit various kinases, and might be used to treat chronic myelocytic leukemia, cancer and neurodegenerative disorders. 7-bromoindirubin-3-oxime (7Bio), an indirubin derivative derived from indirubin-3-oxime, possesses inhibitory effects against cyclin-dependent kinase-5 (CDK5) and glycogen synthase kinase-3β (GSK3β), two pharmacological targets of Alzheimer's disease (AD). In this study, we have discovered that 2.3–23.3 μg/kg 7Bio effectively prevented β-amyloid (Aβ) oligomer-induced impairments of spatial cognition and recognition without affecting bodyweight and motor functions in mice. Moreover, 7Bio potently inhibited Aβ oligomer-induced expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Furthermore, 7Bio significantly prevented the decreased expression of synapsin-1 and PSD-95, biomarkers of pre-synaptic and post-synaptic proteins in Aβ oligomer-treated mice. The mean optical density (OD) with hyper-phosphorylated tau (pTau), glial fibrillary acidic protein (GFAP) and CD45 positive staining in the hippocampus of 7Bio-treated mice were significantly decreased compared to those of Aβ oligomer-treated mice. In addition, Western blotting analysis showed that 7Bio attenuated Aβ oligomer-decreased expression of pSer9-GSK3β. Those results suggested that 7Bio could potently inhibit Aβ oligomer-induced neuroinflammation, synaptic impairments, tau hyper-phosphorylation, and activation of astrocytes and microglia, which may contribute to the neuroprotective effects of 7Bio. Based on these findings, we expected that 7Bio might be developed as a novel anti-AD lead compound.
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- 2017
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6. 9-Methylfascaplysin Is a More Potent Aβ Aggregation Inhibitor than the Marine-Derived Alkaloid, Fascaplysin, and Produces Nanomolar Neuroprotective Effects in SH-SY5Y Cells
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Qingmei Sun, Fufeng Liu, Jingcheng Sang, Miaoman Lin, Jiale Ma, Xiao Xiao, Sicheng Yan, C. Benjamin Naman, Ning Wang, Shan He, Xiaojun Yan, Wei Cui, and Hongze Liang
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fascaplysin ,Alzheimer’s disease ,Aβ ,oligomer ,β-carboline ,Biology (General) ,QH301-705.5 - Abstract
β-Amyloid (Aβ) is regarded as an important pathogenic target for Alzheimer’s disease (AD), the most prevalent neurodegenerative disease. Aβ can assemble into oligomers and fibrils, and produce neurotoxicity. Therefore, Aβ aggregation inhibitors may have anti-AD therapeutic efficacies. It was found, here, that the marine-derived alkaloid, fascaplysin, inhibits Aβ fibrillization in vitro. Moreover, the new analogue, 9-methylfascaplysin, was designed and synthesized from 5-methyltryptamine. Interestingly, 9-methylfascaplysin is a more potent inhibitor of Aβ fibril formation than fascaplysin. Incubation of 9-methylfascaplysin with Aβ directly reduced Aβ oligomer formation. Molecular dynamics simulations revealed that 9-methylfascaplysin might interact with negatively charged residues of Aβ42 with polar binding energy. Hydrogen bonds and π⁻π interactions between the key amino acid residues of Aβ42 and 9-methylfascaplysin were also suggested. Most importantly, compared with the typical Aβ oligomer, Aβ modified by nanomolar 9-methylfascaplysin produced less neuronal toxicity in SH-SY5Y cells. 9-Methylfascaplysin appears to be one of the most potent marine-derived compounds that produces anti-Aβ neuroprotective effects. Given previous reports that fascaplysin inhibits acetylcholinesterase and induces P-glycoprotein, the current study results suggest that fascaplysin derivatives can be developed as novel anti-AD drugs that possibly act via inhibition of Aβ aggregation along with other target mechanisms.
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- 2019
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7. 5-Hydroxycyclopenicillone Inhibits β-Amyloid Oligomerization and Produces Anti-β-Amyloid Neuroprotective Effects In Vitro
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Jiaying Zhao, Fufeng Liu, Chunhui Huang, Jieyi Shentu, Minjun Wang, Chenkai Sun, Liping Chen, Sicheng Yan, Fang Fang, Yuanyuan Wang, Shujun Xu, C. Benjamin Naman, Qinwen Wang, Shan He, and Wei Cui
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5-hydroxycyclopenicillone ,β-amyloid ,Alzheimer’s disease ,sponge-associated fungus ,oligomer ,Organic chemistry ,QD241-441 - Abstract
The oligomer of β-amyloid (Aβ) is considered the main neurotoxin in Alzheimer’s disease (AD). Therefore, the inhibition of the formation of Aβ oligomer could be a target for AD therapy. In this study, with the help of the dot blotting assay and transmission electronic microscopy, it was have discovered that 5-hydroxycyclopenicillone, a cyclopentenone recently isolated from a sponge-associated fungus, effectively reduced the formation of Aβ oligomer from Aβ peptide in vitro. Molecular dynamics simulations suggested hydrophobic interactions between 5-hydroxycyclopenicillone and Aβ peptide, which might prevent the conformational transition and oligomerization of Aβ peptide. Moreover, Aβ oligomer pre-incubated with 5-hydroxycyclopenicillone was less toxic when added to neuronal SH-SY5Y cells compared to the normal Aβ oligomer. Although 5-hydroxycyclopenicillone is not bioavailable in the brain in its current form, further modification or encapsulation of this chemical might improve the penetration of 5-hydroxycyclopenicillone into the brain. Based on the current findings and the anti-oxidative stress properties of 5-hydroxycyclopenicillone, it is suggested that 5-hydroxycyclopenicillone may have potential therapeutic efficacy in treating AD.
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- 2017
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8. Construction and validation of a nomogram based on the log odds of positive lymph nodes to predict the prognosis of T1 gastric cancer
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Xiaqin Chen, Zhijie He, Caiqing Zhao, Kaini Wu, Qi Zhu, Yunfeng Fu, Yating Pan, Yuanping Fan, Sicheng Yang, Yonghua Zeng, Shicheng Luo, Lihua Liu, Fan Du, and Xiaodong Zhou
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Gastric cancer ,Nomogram ,Prognosis ,Cancer-specific survival ,LODDS ,Medicine ,Science - Abstract
Abstract In patients with gastric cancer (GC), metastatic progression through the lymphatic, haematogenous, peritoneal, and ovarian routes is the ultimate cause of death. We developed a nomogram to estimate cancer-specific survival (CSS) in patients with T1 gastric cancer based on log odds of positive lymph nodes (LODDS). A total of 2,221 patients from the Surveillance, Epidemiology, and End Results (SEER) database were split into training and internal validation cohorts, while an external validation cohort included 165 patients from our hospital. Multivariate Cox regression analysis revealed that age, sex, tumour size, LODDS score, and M stage were independent prognostic factors for CSS. The LODDS outperformed the N stage and positive lymph node (PLN) count in terms of predictive ability and is recognised as an independent prognostic factor for nomogram construction. In the training and internal and external validation sets, the 1-year AUCs of the columniogram were 0.732, 0.672, and 0.719, respectively. The 3-year AUCs were 0.705, 0.692, and 0.638, respectively. The 5-year AUCs were 0.726, 0.698, and 0.713, respectively, indicating good predictive power. The calibration curve revealed that the predicted survival rate was consistent with the actual survival rate in the three groups. The ROC and DCA demonstrated that the nomogram has more potential in predicting prognosis than the existing AJCC staging system. We constructed and validated a novel nomogram leveraging LODDS, which effectively estimates the CSS at 1, 3, and 5 years for individuals with gastric cancer.
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- 2025
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9. The Use of Syntax in Chinese: Changchun FAW Historical Old City to Update the Old City Culture
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Sicheng Yan
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- 2022
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10. PLGA-PEG Nanoparticles Facilitate In Vivo Anti-Alzheimer’s Effects of Fucoxanthin, a Marine Carotenoid Derived from Edible Brown Algae
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Wei Cui, Sicheng Yan, C. Benjamin Naman, Zhuoying Wu, Jin Lingli, Xiaojun Yan, Bojun Chen, Hongze Liang, Qiyao Wang, Lingling Zhao, Jinrong Zhang, Mengxiang Yang, Panpan Zhang, Shan He, and Yanfei Xie
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Population ,macromolecular substances ,Xanthophylls ,Pharmacology ,Phaeophyta ,Neuroprotection ,Polyethylene Glycols ,Mice ,chemistry.chemical_compound ,In vivo ,medicine ,Animals ,Fucoxanthin ,education ,Neuroinflammation ,Drug Carriers ,education.field_of_study ,Amyloid beta-Peptides ,technology, industry, and agriculture ,Neurotoxicity ,General Chemistry ,medicine.disease ,Carotenoids ,In vitro ,Bioavailability ,chemistry ,Nanoparticles ,General Agricultural and Biological Sciences - Abstract
The marine natural product fucoxanthin has been reported previously to produce anti-Alzheimer's disease (AD) neuroprotective effects in vitro and in vivo. Fucoxanthin was also demonstrated to be safe in preclinical and small population clinical studies, but the low bioavailability of fucoxanthin in the central nervous system (CNS) has limited its clinical applications. To overcome this, poly lactic-co-glycolic acid-block-polyethylene glycol loaded fucoxanthin (PLGA-PEG-Fuc) nanoparticles with diameter at around 200 nm and negative charge were synthesized and suggested to penetrate into the CNS. Loaded fucoxanthin could be liberated from PLGA-PEG nanoparticles by sustained released in the physiological environment. PLGA-PEG-Fuc nanoparticles were shown to significantly inhibit the formation of Aβ fibrils and oligomers. Moreover, these nanoparticles were taken up by both neurons and microglia, leading to the reduction of Aβ oligomers-induced neurotoxicity in vitro. Most importantly, intravenous injection of PLGA-PEG-Fuc nanoparticles prevented cognitive impairments in Aβ oligomers-induced AD mice with greater efficacy than free fucoxanthin, possibly via acting on Nrf2 and NF-κB signaling pathways. These results altogether suggest that PLGA-PEG nanoparticles can enhance the bioavailability of fucoxanthin and potentiate its efficacy for the treatment of AD, thus potentially enabling its future use for AD therapy.
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- 2021
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11. Exploring the predictive value of serum lipids for short‐term prognosis in patients with acute‐on‐chronic liver failure
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Yunfeng Fu, Kaini Wu, Sicheng Yang, Yuanping Fan, Jianhao Qiu, Qi Zhu, Yating Pan, Xiaqin Chen, Sihai Chen, and Xiaodong Zhou
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acute‐on‐chronic liver failure ,high‐density lipoprotein cholesterol ,serum lipids ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Abstract Aims Acute‐on‐chronic liver failure (ACLF) is defined as acute liver injury superimposed on chronic liver disease, resulting in a significantly increased short‐term mortality rate. Serum lipids are associated with the severity of liver disease and contribute to the prognostic assessment for cirrhosis and liver failure. However, the presentation of serum lipids varies in different studies, making it difficult to draw definitive conclusions. This study aimed to investigate the predictive value of serum lipids for short‐term prognosis in patients with ACLF. Methods This retrospective analysis was conducted using the clinical data of patients hospitalized for ACLF between January 2018 and December 2021. The collected data were subjected to Least Absolute Shrinkage and Selection Operator and logistic regression analyses to identify the independent predictors of 28‐day and 90‐day mortality. Separate regression models were developed for each identified serum lipid. These models were combined with the Model for End‐Stage Liver Disease (MELD), MELD combined with serum sodium concentration (MELD‐Na), Chronic Liver Failure Consortium Acute‐on‐chronic Liver Failure (CLIF‐C ACLF), and Chinese Group on the Study of Severe Hepatitis B‐ACLF (COSSH‐ACLF) scores to construct new models. The performance of these models was evaluated using the area under the receiver operating characteristic curve (AUC), Nagelkerke R2, Brier score, net reclassification index (NRI), and integrated discrimination improvement (IDI) to assess the prognostic ability of serum lipids. Results This study included 266 patients with ACLF, of whom 25.9% (69/266) died within 28 days and 38.5% (102/265, with 1 lost to follow‐up) died within 90 days. The independent predictors of 28‐day mortality were age, the presence of overt hepatic encephalopathy at admission, high‐density lipoprotein cholesterol (HDL‐C) levels, and international normalized ratio (INR). The independent predictors of 90‐day mortality were age, albumin levels, serum creatinine levels, and INR. The comparative analysis revealed that the AUC of all other models exceeded that of HDL‐C (p 0.050). Conclusions Serum HDL‐C is an independent predictor of 28‐day mortality in patients with ACLF. Although it has inferior predictive ability when considered alone, HDL‐C enhances the predictive capacity of MELD, MELD‐Na, and COSSH‐ACLF when incorporated into these models.
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- 2024
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12. Dimeric Tacrine(10)-hupyridone as a Multitarget-Directed Ligand To Treat Alzheimer’s Disease
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Yifan Han, Wei Cui, Yiying Zhou, Yanfei Xie, Karl Wah Keung Tsim, Xinmei Gu, Hui Zhang, Haibo Jin, Sicheng Yan, Marvin Mak, Dongsheng Zhou, Paul R. Carlier, Zhenquan Xuan, and Shengquan Hu
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animal structures ,Physiology ,Cognitive Neuroscience ,Tropomyosin receptor kinase B ,Pharmacology ,Ligands ,Biochemistry ,Neuroprotection ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Alzheimer Disease ,Neurotrophic factors ,medicine ,Animals ,Humans ,Neuroinflammation ,PI3K/AKT/mTOR pathway ,030304 developmental biology ,0303 health sciences ,Amyloid beta-Peptides ,Neurotoxicity ,Cell Biology ,General Medicine ,medicine.disease ,Acetylcholinesterase ,chemistry ,Tacrine ,Cholinesterase Inhibitors ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Alzheimer's disease (AD) is a neurodegenerative disorder with multiple pathological features. Therefore, a multitarget-directed ligands (MTDLs) strategy has been developed to treat AD. We have previously designed and synthesized dimeric tacrine(10)-hupyridone (A10E), a novel tacrine derivative with acetylcholinesterase (AChE) inhibition and brain-derived neurotrophic factor (BDNF) activation activity, by linking tacrine and a fragment of huperzine A. However, it was largely unknown whether A10E could act on other AD targets and produce cognitive-enhancing ability in AD animal models. In this study, A10E could prevent cognitive impairments in APP/PS1 transgenic mice and β-amyloid (Aβ) oligomers-treated mice, with higher potency than tacrine and huperzine A. Moreover, A10E could effectively inhibit Aβ production and deposition, alleviate neuroinflammation, enhance BDNF expression, and elevate cholinergic neurotransmission in vivo. At nanomolar concentrations, A10E could inhibit Aβ oligomers-induced neurotoxicity via the activation of tyrosine kinase receptor B (TrkB)/Akt pathway in SH-SY5Y cells. Furthermore, Aβ oligomerization and fibrillization could be directly disrupted by A10E. Importantly, A10E at high concentrations did not produce obvious hepatotoxicity. Our results indicated that A10E could produce anti-AD neuroprotective effects via the inhibition of Aβ aggregation, the activation of the BDNF/TrkB pathway, the alleviation of neuroinflammation, and the decrease of AChE activity. As MTDLs could produce additional benefits, such as overcoming the deficits of drug combination and enhancing the compliance of AD patients, our results also suggested that A10E might be developed as a promising MTDL lead for the treatment of AD.
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- 2021
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13. Fucoxanthin has potential for therapeutic efficacy in neurodegenerative disorders by acting on multiple targets
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Mengxiang Yang, Jinrong Zhang, Wei Cui, C. Benjamin Naman, Qiyao Wang, Xiaojun Yan, Sicheng Yan, Dongsheng Zhou, Shan He, and Zhenquan Xuan
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0301 basic medicine ,Medicine (miscellaneous) ,Gut flora ,Pharmacology ,Xanthophylls ,medicine.disease_cause ,Neuroprotection ,03 medical and health sciences ,chemistry.chemical_compound ,Protein Aggregates ,0302 clinical medicine ,Nutraceutical ,Functional food ,In vivo ,Fucoxanthin ,Medicine ,Humans ,Prodrugs ,Neuroinflammation ,Clinical Trials as Topic ,030109 nutrition & dietetics ,Nutrition and Dietetics ,biology ,business.industry ,General Neuroscience ,Food Ingredients ,Neurodegenerative Diseases ,General Medicine ,biology.organism_classification ,Carotenoids ,Neuroprotective Agents ,chemistry ,business ,030217 neurology & neurosurgery ,Oxidative stress - Abstract
Fucoxanthin, one of the most abundant carotenoids from edible brown seaweeds, for years has been used as a bioactive dietary supplement and functional food ingredient. Recently, fucoxanthin was reported to penetrate the blood-brain barrier, and was superior to other carotenoids to exert anti-neurodegenerative disorder effects via acting on multiple targets, including amyloid protein aggregation, oxidative stress, neuroinflammation, neuronal loss, neurotransmission dysregulation and gut microbiota disorder. However, the concentration of fucoxanthin required for in vivo neuroprotective effects is somewhat high, and the poor bioavailability of this molecule might prevent its clinical use. As such, new strategies have been introduced to overcome these obstacles, and may help to develop fucoxanthin as a novel lead for neurodegenerative disorders. Moreover, it has been shown that some metabolites of fucoxanthin may produce potent in vivo neuroprotective effects. Altogether, these studies suggest the possibility for future development of fucoxanthin as a one-compound-multiple-target or pro-drug type pharmaceutical or nutraceutical treatment for neurodegenerative disorders.Trial registration: ClinicalTrials.gov identifier: NCT03625284.Trial registration: ClinicalTrials.gov identifier: NCT02875392.Trial registration: ClinicalTrials.gov identifier: NCT03613740.Trial registration: ClinicalTrials.gov identifier: NCT04761406.
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- 2021
14. Thermodynamic Analysis of Eplerenone in 13 Pure Solvents at Temperatures from 283.15 to 323.15 K
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Jianfang Liu, Ting Liu, Rongrong Zhang, Sicheng Yang, Yaoyun Zhang, Chenglingzi Yi, Shuai Peng, and Qing Yang
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Chemistry ,QD1-999 - Published
- 2024
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15. Dimeric Tacrine (10)-Hupyridone Effectively Combats Alzheimer’s Disease as A Multi-Target-Directed Ligand
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Dongsheng Zhou, Yiying Zhou, Zhenquan Xuan, Paul R. Carlier, Haibo Jin, Wei Cui, Sicheng Yan, Shengquan Hu, Karl Wah Keung Tsim, Yifan Han, Yanfei Xie, Hui Zhang, Xingmei Gu, and Marvin Shing-Hung Mak
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animal structures ,Multi target ,Stereochemistry ,Chemistry ,Tacrine ,medicine ,Ligand (biochemistry) ,medicine.drug - Abstract
Background Alzheimer’s disease (AD) is a neurodegenerative disorder with multiple pathological features. Therefore, multi-target-directed ligands (MTDLs) strategy has been developed to combat this disease. We have previously designed and synthesized dimeric tacrine (10)-hupyridone (A10E), a novel tacrine derivative with acetylcholinesterase (AChE) inhibition and brain-derived neurotrophic factor (BDNF) activation activity, by linking tacrine and a fragment of huperzine A. However, it was largely unknown whether A10E could act on other AD targets and produce cognition-enhancing ability in AD animal models. Methods Behavioral and biochemical methods were applied to evaluate multi-target cognitive-enhancing effects and mechanisms of A10E in APP/PS1 transgenic mice and β-amyloid (Aβ) oligomers-treated mice. The neuroprotective mechanisms of A10E were explored in SH-SY5Y cells. And the anti-aggregation effects of A10E on Aβ were directly investigated in vitro. Results A10E could prevent cognitive impairments in both APP/PS1 mice and Aβ oligomers-treated mice, with higher potency than tacrine and huperzine A. Moreover, A10E could effectively inhibit Aβ production and deposition, reduce neuroinflammation, enhance brain derived brain-derived neurotrophic factor (BDNF) expression, and elevate cholinergic neurotransmission in vivo. A10E, at nanomolar concentrations, could also inhibit Aβ oligomers-induced neurotoxicity via the activation of the TrkB/Akt pathway. Furthermore, Aβ oligomerization and fibrillization could be directly disrupted by A10E. Conclusion A10E could produce anti-AD neuroprotective effects via multi-target mechanisms, including the inhibition of Aβ aggregation, the activation of the BDNF/TrkB pathway, the reduction of neuroinflammation and the decrease of AChE activity. As MTDLs could produce additional benefits, such as overcoming the deficits of drug combination and enhancing the compliance of AD patients, our results suggested that A10E might be developed as a promising MTDL lead for the treatment of AD.
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- 2021
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16. Evans Blue Might Produce Pathologically Activated Neuroprotective Effects via the Inhibition of the P2X4R/p38 Signaling Pathway
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Wei Cui, Shazhou Ye, Fufeng Liu, Zhuoying Wu, Xiao Xiao, Mengxiang Yang, Yi Feng, Sicheng Yan, and Jingcheng Sang
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0301 basic medicine ,Brain Infarction ,Male ,Iodoacetic acid ,p38 mitogen-activated protein kinases ,Pharmacology ,Neuroprotection ,Models, Biological ,p38 Mitogen-Activated Protein Kinases ,Cell Line ,Small hairpin RNA ,Rats, Sprague-Dawley ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,medicine ,Animals ,RNA, Messenger ,Evans Blue ,Neurons ,Behavior, Animal ,Cell Death ,Chemistry ,Neurotoxicity ,Infarction, Middle Cerebral Artery ,Cell Biology ,General Medicine ,Hypoxia (medical) ,medicine.disease ,Iodoacetic Acid ,Molecular Docking Simulation ,030104 developmental biology ,Neuroprotective Agents ,medicine.symptom ,Signal transduction ,Receptors, Purinergic P2X4 ,030217 neurology & neurosurgery ,Signal Transduction - Abstract
The main pathological features of ischemic stroke include neuronal damage and blood–brain barrier (BBB) dysfunction. Previous studies have shown that Evans Blue, a dye used to probe BBB integrity, could enter the brain only during the pathological status of ischemic stroke, indicating the potential pathologically activated therapeutic use of this chemical to treat ischemic stroke. In this study, we have reported that Evans Blue could produce in vitro neuroprotective effects against iodoacetic acid (IAA)-induced hypoxia neuronal death in HT22 cells. We further found that P2X purinoreceptor 4 (P2X4R), a subtype of ATP-gated cation channel, was expressed in HT22 cells. Evans Blue could prevent IAA-induced increase of P2X4R mRNA and protein expression. Interestingly, shRNA of P2X4R could protect against IAA-induced activation of p38, and SB203580, a specific inhibitor of p38, could reverse IAA-induced neurotoxicity, indicating that p38 is a downstream signaling molecule of P2X4R. Molecular docking analysis further demonstrated the possible interaction between Evans Blue and the ATP binding site of P2X4R. Most importantly, pre-treatment of Evans Blue could largely reduce neurological and behavioral abnormity, and decrease brain infarct volume in middle cerebral artery occlusion/reperfusion (MCAO) rats. All these results strongly suggested that Evans Blue could exert neuroprotective effects via inhibiting the P2X4R/p38 pathway, possibly by acting on the ATP binding site of P2X4R, indicating that Evans Blue might be further developed as a pathologically activated therapeutic drug against ischemic stroke.
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- 2019
17. 9-Methylfascaplysin exerts anti-ischemic stroke neuroprotective effects via the inhibition of neuroinflammation and oxidative stress in rats
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Chen Xiaowei, Hongze Liang, Hui Zhang, Difan Zhang, Sicheng Yan, Zhenquan Xuan, Wei Cui, Yi Feng, Fei Zhou, Yuechun Mao, Xiaojun Yan, Xinghan Huang, Xiao Xiao, Hao Liu, Hanbo Pan, and Bojun Chen
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Male ,0301 basic medicine ,Indoles ,Inflammasomes ,Immunology ,Pharmacology ,medicine.disease_cause ,Neuroprotection ,Superoxide dismutase ,03 medical and health sciences ,0302 clinical medicine ,NLR Family, Pyrin Domain-Containing 3 Protein ,medicine ,Animals ,Humans ,Immunology and Allergy ,Neuroinflammation ,chemistry.chemical_classification ,biology ,Microglia ,Chemistry ,Glutathione peroxidase ,NF-kappa B ,Infarction, Middle Cerebral Artery ,Inflammasome ,Rats ,Nitric oxide synthase ,Disease Models, Animal ,Oxidative Stress ,Neuroprotective Agents ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Neuroinflammatory Diseases ,biology.protein ,Oxidative stress ,Signal Transduction ,medicine.drug - Abstract
Objectives This study was aimed to investigate the neuroprotective effects of 9-methylfascaplysin, a novel marine derivative derived from sponge, against middle cerebral artery occlusion/reperfusion (MCAO)-induced motor impairments, neuroinflammation and oxidative stress in rats. Methods Neurological and behavioral tests were used to evaluate behavioral changes. The 2, 3, 5-triphenyltetrazolium chloride staining was used to determine infarct size and edema extent. Activated microglia/macrophage was analyzed by immunohistochemical staining of Iba-1. RT-PCR and ELISA were used to measure the expression of inducible nitric oxide synthase, tumor necrosis factor-α, interleukin-1β, CD16 and CD206. Western blotting analysis was performed to explore the activation of nuclear factor-κB (NF-κB) and NLRP3. The levels of oxidative stress were studied by evaluating the activities of superoxide dismutase, catalase and glutathione peroxidase. Results Post-occlusion intracerebroventricular injection of 9-methylfascaplysin significantly attenuated motor impairments and infarct size in MCAO rats. Moreover, 9-methylfascaplysin reduced the activation of microglia/macrophage in ischemic penumbra as evidenced by the decreased Iba-1-positive area and the reduced expression of pro-inflammatory factors. Furthermore, 9-methylfascaplysin inhibited MCAO-induced oxidative stress and activation of NF-κB and NLRP3 inflammasome. Conclusion All the results suggested that 9-methylfascaplysin might produce neuroprotective effects against MCAO via the reduction of oxidative stress and neuroinflammation, simultaneously, possibly via the inhibition of NF-κB and NLRP3 inflammasome.
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- 2021
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18. Sensing characteristics of photonic crystal fiber Sagnac interferometer based on novel birefringence and Vernier effect
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Liang Xing, Sicheng Yan, Zhaoyang Liu, Qiang Liu, and Liangliang Lv
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Interferometry ,Birefringence ,Optics ,Materials science ,business.industry ,General Engineering ,Vernier effect ,business ,Photonic-crystal fiber - Published
- 2020
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19. CSB6B prevents β-amyloid-associated neuroinflammation and cognitive impairments via inhibiting NF-κB and NLRP3 in microglia cells
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Chuang Wang, Wei Cui, Sicheng Yan, Tao Tao, Zhenquan Xuan, Mengxiang Yang, and Qinwen Wang
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0301 basic medicine ,Lipopolysaccharide ,Immunology ,Anti-Inflammatory Agents ,Nitric Oxide Synthase Type II ,Cell Line ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Alzheimer Disease ,NLR Family, Pyrin Domain-Containing 3 Protein ,medicine ,Animals ,Humans ,Immunology and Allergy ,Cognitive Dysfunction ,Viability assay ,Receptor ,Neuroinflammation ,Neurons ,Pharmacology ,Mice, Inbred ICR ,Amyloid beta-Peptides ,Microglia ,biology ,NF-kappa B ,NF-κB ,Trypan Blue ,Nitric oxide synthase ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,030220 oncology & carcinogenesis ,Cancer research ,biology.protein ,Cytokines ,Tumor necrosis factor alpha ,Inflammation Mediators ,Neurogenic Inflammation - Abstract
Pathological β-amyloid (Aβ)-induced microglial activation could cause chronic neuroinflammation in the brain of Alzheimer's disease (AD) patients, and has been considered as one of the main pathological events of this disease. Chicago sky blue 6B (CSB6B), a pigment used in biochemical staining, has been reported to produce analgesic effects in neuroinflammatory-associated pain models. We have previously found that CSB6B could directly inhibit Aβ aggregation and prevent Aβ toxicity in neurons. However, it remains unclear whether this compound could prevent Aβ-induced neuroinflammation and impairments of learning and memory in the AD models. In this study, CSB6B was found to effectively inhibit the production of pro-inflammatory cytokines, including tumor necrosis factor-α and interleukin-1β, without affecting cell viability in BV2 microglia cells stimulated by Aβ oligomer and lipopolysaccharide. Moreover, CSB6B significantly reduced mRNA expression of inducible nitric oxide synthase and increased mRNA expression of arginase-1, suggesting that CSB6B might promote the polarization of BV2 cells into M2 phenotype. In Aβ oligomer-treated mice, hippocampal injection of CSB6B prevented cognitive impairments, and attenuated pro-inflammatory cytokines production. In addition, CSB6B inhibited nuclear transcription factor-κB (NF-κB), and restrainedthe activation of NOD-like receptor pyrin domain containing-3 (NLRP3) both in vitro and in vivo. According to our results, CSB6B may counteract Aβ-induced cognitive impairments and neuroinflammation by inhibiting NF-κB and NLRP3. Combined with previous studies, we anticipated that CSB6B may further develop into a potential anti-AD drug with multiple functions on neurons and microglia cells, concurrently.
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- 2020
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20. 25C-NBOMe, a Novel Designer Psychedelic, Induces Neurotoxicity 50 Times More Potent Than Methamphetamine In Vitro
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Wei Cui, Qiyang Qiu, Peng Xu, Sicheng Yan, Youmei Wang, Hao-Wei Shen, Wenhua Zhou, Haijie Li, C. Benjamin Naman, and Mengxiang Yang
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0301 basic medicine ,MAPK/ERK pathway ,Benzylamines ,Cell Survival ,MAP Kinase Signaling System ,Pharmacology ,Toxicology ,PC12 Cells ,Designer Drugs ,Methamphetamine ,03 medical and health sciences ,chemistry.chemical_compound ,Inhibitory Concentration 50 ,Mice ,0302 clinical medicine ,GSK-3 ,Cell Line, Tumor ,Phenethylamines ,medicine ,Animals ,Humans ,Viability assay ,Propidium iodide ,Protein kinase A ,Neurons ,Kinase ,General Neuroscience ,Neurotoxicity ,medicine.disease ,Rats ,030104 developmental biology ,chemistry ,Hallucinogens ,Proto-Oncogene Proteins c-akt ,030217 neurology & neurosurgery ,medicine.drug - Abstract
25C-NBOMe is a designer substituted phenethylamine and a high-potency psychedelic that acts on the 5-HT2A receptor. Although 25C-NBOMe overdoses have been related to several deaths in the USA and Europe, very limited data exists on the in vitro neurotoxicity of 25C-NBOMe. In this study, we found that 25C-NBOMe potently reduced cell viability of SH-SY5Y, PC12, and SN4741 cells, with IC50 values of 89, 78, and 62 μM, respectively. Methamphetamine decreased the cell viability of these cells with IC50 values at millimolar range in the same tests, indicating that 25C-NBOMe is > 50 times more potent than methamphetamine in its ability to reduce viability of SH-SY5Y cells. The neurotoxicity of 25C-NBOMe on SH-SY5Y cells was further confirmed by using fluorescein diacetate/propidium iodide double staining. 25C-NBOMe elevated the expression of phosphorylated extracellular signal-regulated kinase (pERK), but decreased the expression of phosphorylated Akt and phosphorylated Ser9- glycogen synthase kinase 3β (GSK3β) in time- and concentration-dependent manners. Interestingly, either specific GSK3β inhibitors or specific mitogen-activated protein kinase kinase (MEK) inhibitors significantly prevented 25C-NBOMe-induced neurotoxicity in SH-SY5Y cells. These results suggest that 25C-NBOMe unexpectedly produced more potent neurotoxicity than methamphetamine and that the inhibition of the Akt pathway and activation of the ERK cascade might be involved in 25C-NBOMe-induced neurotoxicity. Most importantly, these findings further inform the toxicity of 25C-NBOMe abuse to the central nervous system for public health.
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- 2018
21. A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis
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Chunhui, Huang, Dilin, Xu, Ke, Zhang, Jieyi, Shentu, Sicheng, Yan, Dapeng, Wu, Qinwen, Wang, and Wei, Cui
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Amyloid beta-Peptides ,Immunoblotting ,Humans ,Biochemistry - Abstract
β-amyloid (Aβ) is a hydrophobic peptide with an intrinsic tendency to self-assemble into aggregates. Among various aggregates, Aβ oligomer is widely accepted as the leading neurotoxin in the progress of Alzheimer's disease (AD) and is considered to be the crucial event in the pathogenesis of AD. Therefore, Aβ oligomer inhibitors might prevent neurodegeneration and have the potential to be developed as disease-modifying treatments of AD. However, different formation protocols of Aβ oligomer might lead to oligomers with different characteristics. Moreover, there are not many methods to effectively screen Aβ(1-42) oligomer inhibitors. An A11 antibody can react with a subset of toxic Aβ(1-42) oligomer with anti-parallel β-sheet structures. In this protocol, we describe how to prepare an A11-positive Aβ(1-42) oligomer-rich sample from a synthetic Aβ(1-42) peptide in vitro and to evaluate relative amounts of A11-positive Aβ(1-42) oligomer in samples by a dot blotting analysis using A11 and Aβ(1-42)-specific 6E10 antibodies. Using this protocol, inhibitors of A11-positive Aβ(1-42) oligomer can also be screened from semi-quantitative experimental results.
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- 2018
22. A vesicular stomatitis virus-based African swine fever vaccine prototype effectively induced robust immune responses in mice following a single-dose immunization
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Yunyun Ma, Junjun Shao, Wei Liu, Shandian Gao, Decai Peng, Chun Miao, Sicheng Yang, Zhuo Hou, Guangqing Zhou, Xuefeng Qi, and Huiyun Chang
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African swine fever virus ,vaccine prototypes ,vesicular stomatitis virus ,safety ,immune potency ,Microbiology ,QR1-502 - Abstract
IntroductionAfrican swine fever (ASF) is a highly contagious hemorrhagic fever disease in pigs caused by African swine fever virus (ASFV). It is very difficult to control and prevent ASF outbreaks due to the absence of safe and effective vaccines.MethodsIn order to develop a safe and effective ASF vaccine for the control and prevention of ASF, two ASFV recombinant vesicular stomatitis virus (VSV) live vector vaccine prototypes, containing the gene of p72, and a chimera of p30 and p54, were developed based on the replication-competent VSV, and named VSV-p72 and VSV-p35. The immune potency of VSV-p72 or VSV-p35 alone and in combination was evaluated in BALB/c mice via intramuscular and intranasal vaccination.ResultsThe results indicated that whether administered alone or in combination, the two vaccine prototypes showed acceptable safety in mice and, more importantly, induced high-level specific antibodies against p72, p30, and p54 of ASFV and a strong cellular immune response 28 days after vaccination. The sera from mice vaccinated with the vaccine prototypes significantly inhibited ASFV from infecting porcine alveolar macrophages (PAMs) in vitro. Most notably, the immunized sera from a mixture of VSV-p35 and VSV-p72 inhibited ASFV from infecting PAMs, with an inhibition rate of up to 78.58%.ConclusionOverall, our findings suggest that ASFV recombinant VSV live vector vaccine prototypes may become a promising candidate vaccine for the control and prevention of ASF.
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- 2024
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23. Enhancing grain drying methods with hyperspectral imaging technology: A visualanalysis
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Sicheng Yang, Yang Cao, Chuanjie Li, Juan Manuel Castagnini, Francisco Jose Barba, Changyao Shan, and Jianjun Zhou
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Grain drying ,Hyperspectral imaging ,Partial least squares model ,Visualization ,Nutrition. Foods and food supply ,TX341-641 ,Food processing and manufacture ,TP368-456 - Abstract
This study proposes a recognition model for different drying methods of grain using hyperspectral imaging technology (HSI) and multivariate analysis. Fresh harvested grain samples were dried using three different methods: rotating ventilation drying, mechanical drying, and natural drying. Hyperspectral images of the samples were collected within the 388–1065 nm band range. The spectral features of the samples were extracted using principal component analysis (PCA), while the texture features were extracted using second-order probability statistical filtering. Partial least squares regression (PLSR) drying models with different characteristics were established. At the same time, a BPNN (Back-propagation neural network, BPNN) based on spectral texture fusion features was established to compare the recognition effects of different models. Texture analysis indicated that the mean-image had the clearest contour, and the texture characteristics of mechanical drying were smaller than those of rotating ventilation drying and natural drying. The BPNN model established using spectral-texture feature variables showed the best performance in distinguishing grain in different drying modes, with a prediction model obtained based on the correlation coefficients of special variables. The spectral and texture feature values were fused for pseudo-color visualization expression, and the three drying methods of grain showed different colors. This study provides a reference for non-destructive and rapid detection of grain with different drying methods.
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- 2024
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24. The Impact of Mixed Ownership Reform on Enterprise Performance–An Empirical Study Based on A-Share Listing Corporation in China
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Sheng Ma, Wenjie Li, and Sicheng Yan
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Solvency ,business.industry ,Restructuring ,media_common.quotation_subject ,05 social sciences ,Accounting ,Monetary economics ,030226 pharmacology & pharmacy ,Corporation ,A share ,03 medical and health sciences ,0302 clinical medicine ,Shareholder ,Debt ,0502 economics and business ,Profitability index ,Listing (finance) ,business ,health care economics and organizations ,050203 business & management ,media_common - Abstract
This paper, with data of 486 listed companies from 2003 to 2014 over the time span of 12 years, finds that the proportion of non state-owned shares in the Fixed-Effect Model is negatively correlated with the profitability of the company, and it is positively correlated with the company’s development capacity; The proportion of circulating stocks has a significant positive impact on the corporate debt solvency and development ability; The proportion of the largest shareholder’s shareholding and the proportion of the top ten shareholders are significantly correlated with the total asset growth rate, while separation rate of two rights and total asset growth rate are negatively correlated. Moreover, by using the Difference-in-Difference Model to test the reform policies, the regression results find that the restructuring has a significantly positive impact on corporate profitability and development capacity. The consistency of two methods confirms that the mixed ownership reform policy has a positive role in promoting the profitability, debt solvency and development capability of state-owned listed companies.
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- 2017
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25. Evaluation of humoral and cellular immune responses induced by a cocktail of recombinant African swine fever virus antigens fused with OprI in domestic pigs
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Guanglei Zhang, Wei Liu, Sicheng Yang, Shuai Song, Yunyun Ma, Guangqing Zhou, Xiaxia Liang, Chun Miao, Junhui Li, Yanhong Liu, Junjun Shao, and Huiyun Chang
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African swine fever virus ,Recombinant fusion proteins ,OprI ,Immune responses ,Vaccine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background African swine fever (ASF) is a highly fatal disease in domestic pigs caused by ASF virus (ASFV), for which there is currently no commercial vaccine available. The genome of ASFV encodes more than 150 proteins, some of which have been included in subunit vaccines but only induce limited protection against ASFV challenge. Methods To enhance immune responses induced by ASFV proteins, we expressed and purified three fusion proteins with each consisting of bacterial lipoprotein OprI, 2 different ASFV proteins/epitopes and a universal CD4+ T cell epitope, namely OprI-p30-modified p54-TT, OprI-p72 epitopes-truncated pE248R-TT, and OprI-truncated CD2v-truncated pEP153R-TT. The immunostimulatory activity of these recombinant proteins was first assessed on dendritic cells. Then, humoral and cellular immunity induced by these three OprI-fused proteins cocktail formulated with ISA206 adjuvant (O-Ags-T formulation) were assessed in pigs. Results The OprI-fused proteins activated dendritic cells with elevated secretion of proinflammatory cytokines. Furthermore, the O-Ags-T formulation elicited a high level of antigen-specific IgG responses and interferon-γ-secreting CD4+ and CD8+ T cells after stimulation in vitro. Importantly, the sera and peripheral blood mononuclear cells from pigs vaccinated with the O-Ags-T formulation respectively reduced ASFV infection in vitro by 82.8% and 92.6%. Conclusions Our results suggest that the OprI-fused proteins cocktail formulated with ISA206 adjuvant induces robust ASFV-specific humoral and cellular immune responses in pigs. Our study provides valuable information for the further development of subunit vaccines against ASF.
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- 2023
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26. A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis
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Chunhui, Huang, primary, Dilin, Xu, primary, Ke, Zhang, primary, Jieyi, Shentu, primary, Sicheng, Yan, primary, Dapeng, Wu, primary, Qinwen, Wang, primary, and Wei, Cui, primary
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- 2018
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27. Prediction of Lubrication Performances of Vegetable Oils by Genetic Functional Approximation Algorithm
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Jianfang Liu, Yaoyun Zhang, Sicheng Yang, Chenglingzi Yi, Ting Liu, Rongrong Zhang, Dan Jia, Shuai Peng, and Qing Yang
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fatty acid ,vegetable oil ,GFA ,QSPR ,lubrication performance ,Science - Abstract
Vegetable oils, which are considered potential lubricants, are composed of different types and proportions of fatty acids. Because of their diverse types and varying compositions, they exhibit different lubrication performances. The genetic function approximation algorithm was used to model the quantitative structure–property relationship between fatty acid structure and the wear scar diameter and friction coefficients measured by four-ball friction and wear tests. Based on the models with adjusted R2 greater than 0.9 and fatty acid compositions of vegetable oils, the wear scar diameter and friction coefficients of Xanthoceras sorbifolia bunge oil and Soybean oil as validation oil samples were predicted. The difference between the predicted and experimental values was small, indicating that the models could accurately predict the lubrication performances of vegetable oils. The lubrication performances of 14 kinds of vegetable oils were predicted by GFA-QSPR models, and the primary factors influencing their lubrication properties were studied by cluster analysis. The results show that the content of C18:1 has a positive effect on the lubrication performances of vegetable oils, while the content of C18:3 has a negative effect, and the length of the carbon chain of fatty acids significantly affects their lubrication properties.
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- 2024
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28. Antigenic and immunogenic properties of recombinant proteins consisting of two immunodominant African swine fever virus proteins fused with bacterial lipoprotein OprI
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Guanglei Zhang, Wei Liu, Zhan Gao, Yanyan Chang, Sicheng Yang, Qian Peng, Sudan Ge, Bijing Kang, Junjun Shao, and Huiyun Chang
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African swine fever virus ,Immunomodulation ,Immune response ,OprI ,Recombinant fusion proteins ,Vaccine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background African swine fever (ASF) is a highly fatal swine disease, which threatens the global pig industry. There is no commercially available vaccine against ASF and effective subunit vaccines would represent a real breakthrough. Methods In this study, we expressed and purified two recombinant fusion proteins, OPM (OprI-p30-modified p54) and OPMT (OprI-p30-modified p54-T cell epitope), which combine the bacterial lipoprotein OprI with ASF virus proteins p30 and p54. Purified recombinant p30 and modified p54 expressed alone or fused served as controls. The activation of dendritic cells (DCs) by these proteins was first assessed. Then, humoral and cellular immunity induced by the proteins were evaluated in mice. Results Both OPM and OPMT activated DCs with elevated expression of relevant surface molecules and proinflammatory cytokines. Furthermore, OPMT elicited the highest levels of antigen-specific IgG responses, cytokines including interleukin-2, interferon-γ, and tumor necrosis factor-α, and proliferation of lymphocytes. Importantly, the sera from mice vaccinated with OPM or OPMT neutralized more than 86% of ASF virus in vitro. Conclusions Our results suggest that OPMT has good immunostimulatory activities and immunogenicity in mice, and might be an appropriate candidate to elicit immune responses in swine. Our study provides valuable information on further development of a subunit vaccine against ASF.
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- 2022
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29. Identification of p72 epitopes of African swine fever virus and preliminary application
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Chun Miao, Sicheng Yang, Junjun Shao, Guangqing Zhou, Yunyun Ma, Shenghui Wen, Zhuo Hou, Decai Peng, HuiChen Guo, Wei Liu, and Huiyun Chang
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African swine fever virus ,p72 ,monoclonal antibodies ,epitope ,diagnosis ,Microbiology ,QR1-502 - Abstract
African swine fever virus (ASFV) causes a highly lethal hemorrhagic viral disease (ASF) of pigs that results in serious losses in China and elsewhere. The development of a vaccine and diagnosis technology for ASFV is essential to prevent and control the spread of ASF. The p72 protein of ASFV is highly immunogenic and reactive, and is a dominant antigen in ASF vaccine and diagnostic research. In this study, 17 p72 monoclonal antibodies (mAbs) were generated. Epitope mapping by a series of overlapping peptides expressed in Escherichia coli showed that these mAbs recognized a total of seven (1–7) linear B cell epitopes. These mAbs did not show significant neutralizing activity. Epitopes 1 (249HKPHQSKPIL258), 2 (69PVGFEYENKV77), 5 (195VNGNSLDEYSS205), and 7 (223GYKHLVGQEV233) are novel. Sequence alignment analysis revealed that the identified epitopes were highly conserved among 27 ASFV strains from nine genotypes. Preliminary screening using known positive and negative sera indicated the diagnostic potential of mAb-2B8D7. The results provide new insights into the antigenic regions of ASFV p72 and will inform the diagnosis of ASFV.
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- 2023
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30. Structure and function of African swine fever virus proteins: Current understanding
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Sicheng Yang, Chun Miao, Wei Liu, Guanglei Zhang, Junjun Shao, and Huiyun Chang
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African swine fever ,African swine fever virus ,structural proteins ,non-structural proteins ,capsid protein ,inhibitor of apoptosis protein ,Microbiology ,QR1-502 - Abstract
African swine fever virus (ASFV) is a highly infectious and lethal double-stranded DNA virus that is responsible for African swine fever (ASF). ASFV was first reported in Kenya in 1921. Subsequently, ASFV has spread to countries in Western Europe, Latin America, and Eastern Europe, as well as to China in 2018. ASFV epidemics have caused serious pig industry losses around the world. Since the 1960s, much effort has been devoted to the development of an effective ASF vaccine, including the production of inactivated vaccines, attenuated live vaccines, and subunit vaccines. Progress has been made, but unfortunately, no ASF vaccine has prevented epidemic spread of the virus in pig farms. The complex ASFV structure, comprising a variety of structural and non-structural proteins, has made the development of ASF vaccines difficult. Therefore, it is necessary to fully explore the structure and function of ASFV proteins in order to develop an effective ASF vaccine. In this review, we summarize what is known about the structure and function of ASFV proteins, including the most recently published findings.
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- 2023
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31. Evaluation of Antioxidant Properties and Molecular Design of Lubricant Antioxidants Based on QSPR Model
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Jianfang Liu, Yaoyun Zhang, Chenglingzi Yi, Rongrong Zhang, Sicheng Yang, Ting Liu, Dan Jia, Qing Yang, and Shuai Peng
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QSPR ,lubricating oil antioxidant ,antioxidant property ,MLR ,HOMO–LUMO ,Science - Abstract
Two quantitative structure–property relationship (QSPR) models of hindered phenolic antioxidants in lubricating oils were established to help guide the molecular structure design of antioxidants. Firstly, stepwise regression (SWR) was used to filter out essential molecular descriptors without autocorrelation, including electronic, topological, spatial, and structural descriptors, and multiple linear regression (MLR) was used to construct QSPR models based on the screened variables. The two models are statistically sound, with R2 values of 0.942 and 0.941, respectively. The models’ reliability was verified by the frontier molecular orbital energy gaps of the antioxidants. A hindered phenolic additive was designed based on the models. Its antioxidant property is calculated to be 20.9% and 11.0% higher than that of typical commercial antioxidants methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate and 2,2′-methylenebis(6-tert-butyl-4-methylphenol), respectively. The structure–property relationship of hindered phenolic antioxidants in lubricating oil obtained by computer-assisted analysis can not only predict the antioxidant properties of existing hindered phenolic additives but also provide theoretical basis and data support for the design or modification of lubricating oil additives with higher antioxidant properties.
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- 2023
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32. Development of a competitive chemiluminescence immunoassay using a monoclonal antibody recognizing 3B of foot-and-mouth disease virus for the rapid detection of antibodies induced by FMDV infection
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Wei Liu, Guanglei Zhang, Sicheng Yang, Junhui Li, Zhan Gao, Sudan Ge, Huihui Yang, Junjun Shao, and Huiyun Chang
- Subjects
Chemiluminescence immunoassay ,Diagnosis ,Foot-and-mouth disease virus ,Monoclonal antibody ,Non-structural protein ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Foot-and-mouth disease (FMD) is a devastating animal disease. Anti-non-structural protein (NSP) antibody detection is very important for confirming suspected cases, evaluating the prevalence of infection, certifying animals for trade and controlling the disease. Methods In this study, a competitive chemiluminescence immunoassay (3B-cCLIA) was developed for the rapid detection of antibodies against NSPs in different species of livestock animals using the monoclonal antibody (mAb) 9E2 as a competitive antibody that recognizes NSP 3B. Results The cut-off value (50%), diagnostic sensitivity (Dsn) (97.20%, 95.71%, and 96.15%) and diagnostic specificity (Dsp) (99.51%, 99.43%, and 98.36) of the assay were estimated by testing a panel of known-background sera from swine, cattle and sheep, respectively. The accuracy rate of the 3B-cCLIA was further validated and subsequently compared with that of two commercial diagnostic kits. The early diagnostic results showed that antibodies recognizing NSPs developed later (approximately 1–2 days) than antibodies recognizing structural proteins. Furthermore, anti-NSP antibody presence in animals vaccinated multiple times (false positives), especially cattle and sheep, was confirmed, and the false-positive rate increased with the number of vaccinations. Conclusions These results indicate that the 3B-cCLIA is suitable for the rapid detection of antibodies against FMDV NSP 3B in a wide range of species.
- Published
- 2021
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