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4 results on '"Sieglova K"'

1. Analysis of DNA methylation and microRNA expression in NUT (nuclear protein in testis) midline carcinoma of the sinonasal tract: a clinicopathological, immunohistochemical and molecular genetic study

Author

LACO, J., primary, KOVARIKOVA, H., additional, CHMELAROVA, M., additional, VOSMIKOVA, H., additional, SIEGLOVA, K., additional, BUBANCOVA, I., additional, DUNDR, P., additional, NEMEJCOVA, K., additional, MICHALEK, J., additional, CELAKOVSKY, P., additional, MOTTL, R., additional, SIRAK, I., additional, VOSMIK, M., additional, MAREK, I., additional, GERYK, T., additional, MEJZLIK, J., additional, SATANKOVA, J., additional, and RYSKA, A., additional

Published
2018
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3. Importance of Tumour Suppressor Gene Methylation in Sinonasal Carcinomas

Author

Chmelarova, M., Sirak, I., Mzik, M., Sieglova, K., Vosmikova, H., Pavel Dundr, Nemejcova, K., Michalek, J., Vosmik, M., Palicka, V., and Laco, J.

Subjects
DNA (Cytosine-5-)-Methyltransferase 1, Epigenomics, Squamous Cell Carcinoma of Head and Neck, DNA Methylation, Cadherins, Prognosis, Enzyme Activation, Head and Neck Neoplasms, Carcinoma, Squamous Cell, Humans, Genes, Tumor Suppressor, DNA (Cytosine-5-)-Methyltransferases, Promoter Regions, Genetic, Papillomaviridae
Abstract

Epigenetic changes are considered to be a frequent event during tumour development. Hypermethylation of promoter CpG islands represents an alternative mechanism for inactivation of tumour suppressor genes, DNA repair genes, cell cycle regulators and transcription factors. The aim of this study was to investigate promoter methylation of specific genes in samples of sinonasal carcinoma by comparison with normal sinonasal tissue. To search for epigenetic events we used methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) to compare the methylation status of 64 tissue samples of sinonasal carcinomas with 19 control samples. We also compared the human papilloma virus (HPV) status with DNA methylation. Using a 20% cut-off for methylation, we observed significantly higher methylation in RASSF1, CDH13, ESR1 and TP73 genes in the sinonasal cancer group compared with the control group. HPV positivity was found in 15/64 (23.4 %) of all samples in the carcinoma group and in no sample in the control group. No correlation was found between DNA methylation and HPV status. In conclusion, our study showed that there are significant differences in promoter methylation in the RASSF1, ESR 1, TP73 and CDH13 genes between sinonasal carcinoma and normal sinonasal tissue, suggesting the importance of epigenetic changes in these genes in carcinogenesis of the sinonasal area. These findings could be used as prognostic factors and may have implications for future individualised therapies based on epigenetic changes.

4. Deregulation of selected microRNAs in sinonasal carcinoma: Value of miR-21 as prognostic biomarker in sinonasal squamous cell carcinoma.

Author

Kovarikova H, Bubancova I, Laco J, Sieglova K, Vosmikova H, Vosmik M, Dundr P, Nemejcova K, Michalek J, Palicka V, and Chmelarova M

Subjects
Aged, Analysis of Variance, Biomarkers, Tumor genetics, Biopsy, Needle, Carcinoma, Squamous Cell genetics, Carcinoma, Squamous Cell mortality, Carcinoma, Squamous Cell surgery, Case-Control Studies, Female, Gene Expression Profiling, Humans, Immunohistochemistry, Kaplan-Meier Estimate, Male, Middle Aged, Paranasal Sinus Neoplasms mortality, Paranasal Sinus Neoplasms surgery, Prognosis, Real-Time Polymerase Chain Reaction methods, Reproducibility of Results, Statistics, Nonparametric, Survival Analysis, Up-Regulation, Carcinoma, Squamous Cell pathology, Gene Expression Regulation, Neoplastic, MicroRNAs genetics, Paranasal Sinus Neoplasms genetics, Paranasal Sinus Neoplasms pathology
Abstract

Background: Tumors occurring in the sinonasal area are characterized by unfavorable outcome due to difficult diagnosis, treatment, and prognosis of the disease corresponding with the anatomic complexity of the area., Methods: We used quantitative real-time polymerase chain reaction (PCR) to compare relative expression of miR-21, miR-141, and miR-200c in 70 formalin-fixed, paraffin-embedded samples of sinonasal carcinoma tissue (majority of squamous cell carcinoma [SCC] samples) with 17 control samples of sinonasal tissue., Results: Our data showed significant upregulation of miR-21 in sinonasal cancer tissue. Expression levels of miR-141 and miR-200c were below detectable levels in both sinonasal cancer samples and healthy tissue. Kaplan-Meier analysis with log-rank survival showed that patients with SCC with high expression of miR-21 (highest quartile) had impaired survival close to reaching statistical significance (Pā€‰=ā€‰.0630)., Conclusion: Our results suggest that miR-21 upregulation is involved in tumorigenesis of sinonasal carcinoma and that it is associated with poor prognosis. Thus, miR-21 could be used as a valuable prognostic biomarker., (© 2017 Wiley Periodicals, Inc.)

Published
2017
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