1. Development of an integrated breath analysis technology for on-chip aerosol capture and molecular analysis
- Author
-
Filip Paeps, Thomas Degreef, Wout Duthoo, Yorick Koumans, Erik Emmen, Abdulkadir Yurt, Elisabeth Marchal, Andrey Kossarev, Thi-Minh-Tho Dam, Rabea Hanifa, Joost Van Duppen, Zhenxiang Luo, Marco Peca, Tobe Wauters, Wouter Vleugels, Nadia Chakrova, Johan Berte, Floris Vernieuwe, Maxime Delgrange, Hari Prasanth, Sven Van Geyt, Frederik Mortier, Joren Raymenants, Emmanuel André, Nik Van den Wijngaert, Peter Peumans, Tim Stakenborg, and Bert Verbruggen
- Subjects
Breath sampling ,Fast qPCR ,Silicon impactor ,Aerosols ,COVID-19 ,In-vitro diagnosis ,Electronics ,TK7800-8360 ,Technology (General) ,T1-995 - Abstract
As proven early on in the pandemic, SARS-CoV-2 is mainly transmitted by aerosols. This urged us to develop a silicon impactor that collects the virus particles directly from breath. Performing PCR on these breath samples proved equally sensitive as nasopharyngeal swabs during the first week of an infection [Stakenborg et al., 2022], yet it remained a mostly manual process and PCR turn-around-time was still long. To overcome these drawbacks, we developed a fast and sensitive, fully integrated point-of-need breath test, comprising a novel breath sampler device and PCR instrument. The breath sampler combines virus collection and in-situ RNA amplification. The PCR instrument performs very fast amplification of the released viral RNA. Sample-to-result time was reduced to
- Published
- 2023
- Full Text
- View/download PDF