1. Caveolin-1 deficiency alters plasma lipid and lipoprotein profiles in mice
- Author
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Philipp Wiesner, Silke Lindtner, Susanne Heimerl, Kai Simons, Soazig Le Lay, Teymuras V. Kurzchalia, Gerhard Liebisch, Alfred Böttcher, Gerd Schmitz, Stress Oxydant et Pathologies Métaboliques (SOPAM), and Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)
- Subjects
Apolipoprotein E ,medicine.medical_specialty ,Ceramide ,Knockout ,[SDV]Life Sciences [q-bio] ,Lipoproteins ,Caveolin 1 ,Biophysics ,Inbred C57BL ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Internal medicine ,Caveolae ,medicine ,Animals ,Molecular Biology ,030304 developmental biology ,Mice, Knockout ,0303 health sciences ,Cholesterol ,Reverse cholesterol transport ,Lipid metabolism ,Cell Biology ,Fasting ,Lipid Metabolism ,Postprandial Period ,Dietary Fats ,Lipids ,Mice, Inbred C57BL ,Endocrinology ,chemistry ,Cholesteryl ester ,cardiovascular system ,lipids (amino acids, peptides, and proteins) ,030217 neurology & neurosurgery ,Lipoprotein - Abstract
International audience; Caveolae are specialized membrane microdomains formed as the result of local accumulation of cholesterol, glycosphingolipids, and the structural protein caveolin-1 (Cav-1). To further elucidate the role of Cav-1 in lipid homeostasis in-vivo, we analyzed fasting and post-prandial plasma from Cav-1 deficient mice on low or on high fat diet. In total plasma analysis, an increase in ceramide and hexosylceramide was observed. In cholesteryl ester (CE), we found an increased saturated+monounsaturated/polyunsaturated fatty acid ratio in fasting plasma of low fat fed Cav-1(-/-) mice with increased proportions of CE16:1, CE18:1, CE20:3, and decreased proportions of CE18:2 and CE22:6. Under high fat diet HDL-CE, free cholesterol and pre-beta-HDL were increased accompanied by a shift from slow to fast migrating alpha-HDL and expansion of apoE containing HDL. Our results demonstrate a significant role of Cav-1 in HDL-cholesterol metabolism and may reflect a variety of Cav-1 functions including modulation of ACAT activity and SR-BI function.
- Published
- 2008
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