1. Sequencing and Description of the Mitochondrial Genome of Orthopodomyia fascipes (Diptera: Culicidae).
- Author
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Silva, Fábio Silva da, Nascimento, Bruna Laís Sena do, Cruz, Ana Cecília Ribeiro, Silva, Sandro Patroca da, Aragão, Carine Fortes, Dias, Daniel Damous, Silva, Lucas Henrique da Silva e, Reis, Lúcia Aline Moura, Reis, Hanna Carolina Farias, Chagas, Liliane Leal das, Rosa Jr., José Wilson, Vieira, Durval Bertram Rodrigues, Brandão, Roberto Carlos Feitosa, Medeiros, Daniele Barbosa de Almeida, and Nunes Neto, Joaquim Pinto
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MITOCHONDRIAL DNA , *NUCLEOTIDE sequencing , *BAYESIAN field theory , *DIPTERA , *PHYLOGENY - Abstract
The genus Orthopodomyia Theobald, 1904 (Diptera: Culicidae) comprises 36 wild mosquito species, with distribution largely restricted to tropical and temperate areas, most of which are not recognized as vectors of epidemiological importance due to the lack of information related to their bionomy and involvement in the cycle transmission of infectious agents. Furthermore, their evolutionary relationships are not completely understood, reflecting the scarcity of genetic information about the genus. Therefore, in this study, we report the first complete description of the mitochondrial genome of a Neotropical species representing the genus, Orthopodomyia fascipes Coquillet, 1906, collected in the Brazilian Amazon region. Using High Throughput Sequencing, we obtained a mitochondrial sequence of 15,598 bp, with an average coverage of 418.5×, comprising 37 functional subunits and a final portion rich in A + T, corresponding to the control region. The phylogenetic analysis, using Maximum Likelihood and Bayesian Inference based on the 13 protein-coding genes, corroborated the monophyly of Culicidae and its two subfamilies, supporting the proximity between the tribes Orthopodomyiini and Mansoniini, partially disagreeing with previous studies based on the use of molecular and morphological markers. The information generated in this study contributes to a better understanding of the taxonomy and evolutionary history of the genus and other groups of Culicidae. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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