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135 results on '"Silvestroni, Leopoldo"'

9. A comprehensive approach to the investigation of atmospheric particulate PM2.5: preliminary results. 2002

Author

Gianfagna, Antonio, Silvestroni, Leopoldo, Sacco, Fabrizio, Piga, Luigi, Palleschi, Simonetta, Nardi, Elisa, Montereali, Maria Rita, Mazziotti-Tagliani, Simona, Inglessis, Marco, Armiento, Giovanna, Gianfagna, Antonio, Silvestroni, Leopoldo, Sacco, Fabrizio, Piga, Luigi, Palleschi, Simonetta, Nardi, Elisa, Montereali, Maria Rita, Mazziotti-Tagliani, Simona, Inglessis, Marco, and Armiento, Giovanna

Subjects
heavy metals leaching, PM2.5, particulate matter characterization, multi-method approach, Rome urban area
Abstract

Understanding the health effects induced in humans by the inhalation of air particulate matter requires first that airborne particles be characterized in detail. Aiming at elucidating some prominent mechanisms and processes involved in the atmospheric environment - human organism interaction, a comprehensive approach was adopted to thoroughly investigate PM2.5 from a mineralogical, chemical and chemical-physical point of view. In the present study, two summer and winter samplings collected from the Rome urban area were investigated by different techniques (SEM-EDS, XRD, ICP-MS, ICP-OES, TGA-DTA). Mineralogical and physical characterization gave important information on the prevalent phases (oxides, sulphates, silicates, carbonates, and abundant carbonaceous particles hosting metals) contained into the material under study. The bulk chemical composition evidenced the presence of heavy metals, in particular As, Cd, Cu, Cr, Fe, Pb, Ni, and Zn. Consequently, in order to verify the potential mobility of such heavy metals, batch kinetic leaching tests were carried out by using a physiological solution mimicking the lung environment and samples were collected from the solution at different times of treatment. Among the metals contained into the particulate, a leaching trend was observed mainly for Pb, Cd and Cu, with Cd resulting the most mobile metal, whereas no Fe release was observed. The leaching solution mimicking the human intrapulmonary fluid will be exploited for future toxicological tests in cellular models, in order to assess the potentially harmful role of the above metals in biological systems.

Published
2013

10. Modificazioni endocrine nell’aging male

Author

Vena, W, Sansone, Andrea, Sansone, M, Lallo, D, Sonaglia, A, Intrieri, Francesco, Silvestroni, Leopoldo, and Romanelli, Francesco

Subjects
ipogonadismo, andropausa, ormone della crescita, DHEAS, testosterone, Invecchiamento, somatopausa, DHEA, adrenopausa
Published
2014

23. Neuronal glycolytic pathway impairment induced by HIV envelope glycoprotein gp120

Author

Vignoli, A. L., Martini, I., Haglid, K. G., Silvestroni, Leopoldo, Augusti Tocco, G., and Biagioni, Stefano

Subjects
Time Factors, Cell Survival, Immunoblotting, Galactosylceramides, CHO Cells, HIV Envelope Protein gp120, Fluorescence, Mice, Neuroblastoma, Oxygen Consumption, Cricetinae, Tumor Cells, Cultured, Animals, Fluorescent Dyes, Neurons, Microscopy, Animals, CHO Cells, Cell Membrane, metabolism, Cell Survival, drug effects, Cricetinae, Fluorescent Dyes, pharmacology, Galactosylceramides, metabolism, Glycolysis, HIV Envelope Protein gp120, metabolism/pharmacology, Immunoblotting, Mice, Microscopy, Fluorescence, Neuroblastoma, drug therapy, Neurons, drug effects/enzymology/metabolism, Oxygen Consumption, drug effects, Phosphopyruvate Hydratase, metabolism, Time Factors, Tumor Cells, Cultured, Cell Membrane, metabolism/pharmacology, drug therapy, Tumor Cells, Microscopy, Fluorescence, drug effects, drug effects/enzymology/metabolism, Phosphopyruvate Hydratase, pharmacology, metabolism, Glycolysis
Abstract

Neurological impairment is a common feature of Acquired Immunodeficiency Syndrome (AIDS); functional alterations have been reported both in central and peripheral nervous system and the Human Immunodeficiency Virus (HIV) envelope glycoprotein gp120 has been proposed as a neurotoxin acting through a calcium-dependent mechanism. On the other hand it has been reported that gp120 treatment also induce about a 20% decrease in the cerebral glucose utilization and in the cellular ATP levels. The reported observations were performed on experimental system where also non-neuronal cells where present; in order to evaluate whether a direct interaction between HIV proteins and neuronal cells takes place, we used a neuroblastoma cultures where only neuronal cells are present. We analysed the effects of gp120 on the N18TG2 neuroblastoma clone. Treatments were performed both on growing and confluent cultures. Short time treatment with gp120 of confluent cultures causes a 25% reduction in the level of neuron-specific enolase, resulting in a similar decrease of oxygen consumption. Long time exposure of growing cells also causes a reduction in cell survival. Furthermore, using a membrane-specific fluorescent probe we observed that gp120 produces an increase of membrane trafficking. These observations suggest a direct interaction between the viral envelope protein and neuronal cells, which results in an alteration of glycolytic metabolism. This alteration may be related to the neurologic impairments observed in AIDS patients.

Published
2001

27. S.O.S. Fertilità

Author

Silvestroni, Leopoldo

Subjects
Inquinamento ambientale, fertilità maschile, interferenti endocrini
Published
1998

28. AFM study of human spermatozoa

Author

Altavilla, M., Palleschi, S., Silvestroni, Leopoldo, Carosi, A., Braucci, M., and Coluzza, C.

Published
1998

39. Thyroid hormone and membrane fluidity in rat Sertoli cells

Author

Jannini, Ea, Palleschi, S, Silvestroni, Leopoldo, Ulisse, Salvatore, Rucci, N, Piersanti, D, Graziano, Fm, Carosa, E, Mazzone, D, and D'Armiento, Massimino

Subjects
cell membrane fluidity, Thyroid hormo, Testis, Rat, Sertoli cell
Published
1995

44. Obesità

Author

Fabbrini, A. and Silvestroni, Leopoldo

Published
1994

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