26 results on '"Simone Schiavo"'
Search Results
2. Pulmonary function following hyperbaric oxygen therapy: A longitudinal observational study
- Author
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Connor T. A. Brenna, Shawn Khan, George Djaiani, Darren Au, Simone Schiavo, Mustafa Wahaj, Ray Janisse, and Rita Katznelson
- Subjects
Medicine ,Science - Abstract
Hyperbaric oxygen therapy (HBOT) is known to be associated with pulmonary oxygen toxicity. However, the effect of modern HBOT protocols on pulmonary function is not completely understood. The present study evaluates pulmonary function test changes in patients undergoing serial HBOT. We prospectively collected data on patients undergoing HBOT from 2016–2021 at a tertiary referral center (protocol registration NCT05088772). Patients underwent pulmonary function testing with a bedside spirometer/pneumotachometer prior to HBOT and after every 20 treatments. HBOT was performed using 100% oxygen at a pressure of 2.0–2.4 atmospheres absolute (203–243 kPa) for 90 minutes, five times per week. Patients’ charts were retrospectively reviewed for demographics, comorbidities, medications, HBOT specifications, treatment complications, and spirometry performance. Primary outcomes were defined as change in percent predicted forced expiratory volume in one second (FEV1), forced vital capacity (FVC), and forced mid-expiratory flow (FEF25-75), after 20, 40, and 60 HBOT sessions. Data was analyzed with descriptive statistics and mixed-model linear regression. A total of 86 patients were enrolled with baseline testing, and the analysis included data for 81 patients after 20 treatments, 52 after 40 treatments, and 12 after 60 treatments. There were no significant differences in pulmonary function tests after 20, 40, or 60 HBOT sessions. Similarly, a subgroup analysis stratifying the cohort based on pre-existing respiratory disease, smoking history, and the applied treatment pressure did not identify any significant changes in pulmonary function tests during HBOT. There were no significant longitudinal changes in FEV1, FVC, or FEF25-75 after serial HBOT sessions in patients regardless of pre-existing respiratory disease. Our results suggest that the theoretical risk of pulmonary oxygen toxicity following HBOT is unsubstantiated with modern treatment protocols, and that pulmonary function is preserved even in patients with pre-existing asthma, chronic obstructive lung disease, and interstitial lung disease.
- Published
- 2023
3. Mechanistic Rationale and Clinical Efficacy of Hyperbaric Oxygen Therapy in Chronic Neuropathic Pain: An Evidence-Based Narrative Review
- Author
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Simone Schiavo, Julian DeBacker, Carine Djaiani, Anuj Bhatia, Marina Englesakis, and Rita Katznelson
- Subjects
Medicine (General) ,R5-920 - Abstract
Background. Chronic neuropathic pain is a condition affecting an increasing proportion of the general population and its management requires a comprehensive, multidisciplinary program. A growing body of evidence supports the use of hyperbaric oxygen therapy (HBOT) in several chronic neuropathic pain conditions; however, its role and efficacy remain unclear. Purpose. To summarize current evidence for the mechanistic rationale of HBOT in chronic neuropathic pain conditions and to evaluate its clinical efficacy. Methods. This narrative review was conducted after searching the following databases (Medline, Embase, Cochrane, PsycINFO, the Web of Science, Scopus, ClinicalTrials. gov, WHO ICTRP, and ProQuest Digital Dissertation) from January 1946 to March 2020. Articles published in English that involved either animal or human studies with acute or chronic neuropathic pain evaluating any HBOT-related intervention were included. Results. A total of 2971 citations were identified. A total of 29 studies were included in this review. The mechanisms of action for HBOT use in neuropathic conditions included the primary effects of hyperoxia and edema resolution, as well as the secondary effects pertinent to the production of oxygen and nitrogen reactive species (serving as pain signaling molecules), nitric oxide-dependent release of opioid peptides, and reduction of inflammatory mediators. A robust evidence for HBOT use in the clinical setting was associated with chronic regional pain syndrome and chronic primary bladder pain syndrome. Some evidence supported its use for chronic secondary (peripheral) neuropathic pain including radiation-induced plexus neuropathies, postherpetic neuralgia, and trigeminal neuralgia. Conclusions. HBOT has been shown to have antinociceptive and analgesic effects in animal models of inflammatory, neuropathic, and chronic pain. Human studies demonstrated beneficial effects of HBOT in improving clinical outcomes such as pain scores, pain-related symptoms, and quality of life. A systematic methodology of HBOT application is necessary to confirm its safety and efficacy.
- Published
- 2021
- Full Text
- View/download PDF
4. Arterial Blood Gas Analysis in Breath-Hold Divers at Depth
- Author
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Gerardo Bosco, Alex Rizzato, Luca Martani, Simone Schiavo, Ennio Talamonti, Giacomo Garetto, Matteo Paganini, Enrico M. Camporesi, and Richard E. Moon
- Subjects
arterial blood gas ,blood gas analysis ,breath-hold diving ,physiology ,underwater ,Physiology ,QP1-981 - Abstract
The present study aimed to evaluate the partial pressure of arterial blood gases in breath-hold divers performing a submersion at 40 m. Eight breath-hold divers were enrolled for the trials held at “Y-40 THE DEEP JOY” pool (Montegrotto Terme, Padova, Italy). Prior to submersion, an arterial cannula in the radial artery of the non-dominant limb was positioned. All divers performed a sled-assisted breath-hold dive to 40 m. Three blood samplings occurred: at 10 min prior to submersion, at 40 m depth, and within 2 min after diver’s surfacing and after resuming normal ventilation. Blood samples were analyzed immediately on site. Six subjects completed the experiment, without diving-related problems. The theoretically predicted hyperoxia at the bottom was observed in 4 divers out of 6, while the other 2 experienced a reduction in the partial pressure of oxygen (paO2) at the bottom. There were no significant increases in arterial partial pressure of carbon dioxide (paCO2) at the end of descent in 4 of 6 divers, while in 2 divers paCO2 decreased. Arterial mean pH and mean bicarbonate (HCO3−) levels exhibited minor changes. There was a statistically significant increase in mean arterial lactate level after the exercise. Ours was the first attempt to verify real changes in blood gases at a depth of 40 m during a breath-hold descent in free-divers. We demonstrated that, at depth, relative hypoxemia can occur, presumably caused by lung compression. Also, hypercapnia exists at depth, to a lesser degree than would be expected from calculations, presumably because of pre-dive hyperventilation and carbon dioxide distribution in blood and tissues.
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- 2018
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5. A specialized airway management team for COVID-19 patients: a retrospective study of the experience of two Canadian hospitals in Toronto
- Author
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Nicola Jarvis, Simone Schiavo, Justyna Bartoszko, Martin Ma, Ki Jinn Chin, and Matteo Parotto
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Canada ,airway management ,Anesthesiology and Pain Medicine ,SARS-CoV-2 ,Intubation, Intratracheal ,COVID-19 ,Humans ,Prospective Studies ,General Medicine ,Pandemics ,Hospitals ,Reports of Original Investigations ,Retrospective Studies - Abstract
In the COVID-19 pandemic, an unprecedented number of individuals required endotracheal intubation. To safely face these challenges, expert intubation teams were formed in some institutions. Here, we report on the experience of emergency rapid intubation teams (ERITs) in two Canadian hospitals.We retrospectively collected data on all airway management procedures in confirmed or suspected COVID-19 patients performed by ERITs at two academic hospitals between 3 April and 17 June 2020. The co-primary outcomes were incidence of periprocedural adverse events (hypoxemia, hypotension, and cardiac arrest within 15 min of intubation) and first-attempt intubation success rate. Secondary outcomes included number of intubation attempts, device used to achieve successful airway management, and adherence to personal protective equipment (PPE) protocols.During the study period, 123 patients were assessed for airway management, with 117 patients receiving airway interventions performed by the ERIT. The first-attempt success rate for intubation was 92%, and a videolaryngoscope was the final successful device in 93% of procedures. Hypoxemia (peripheral oxygen saturation [SpOIn this cohort of critically ill patients with respiratory failure requiring time-sensitive airway management, specialized ERIT teams showed high rates of successful airway management with high adherence to PPE use. Hypoxemia and hemodynamic instability were common and should be anticipated within the first 15 min following intubation.www.ClinicalTrials.gov (NCT04689724); registered 30 December 2020.RéSUMé: CONTEXTE: Pendant la pandémie de COVID-19, un nombre sans précédent de patients ont dû bénéficier d’une intubation endotrachéale. Pour faire face en toute sécurité à ces défis, des équipes d’experts en intubation ont été formées dans certains établissements. Nous rendons compte ici de l’expérience d’équipes d’intubation rapide d’urgence (ou ERIT, pour Emergency Rapid Intubation Team) dans deux hôpitaux canadiens. MéTHODE: Nous avons colligé rétrospectivement les données concernant toutes les interventions de prise en charge des voies aériennes chez les patients COVID-19 confirmés ou suspectés réalisées par les ERIT dans deux hôpitaux universitaires entre le 3 avril et le 17 juin 2020. Les deux critères d’évaluation principaux étaient l’incidence d’événements indésirables péri-procédure (hypoxémie, hypotension et arrêt cardiaque dans les 15 minutes suivant l’intubation) et le taux de réussite de l’intubation à la première tentative. Les critères d’évaluation secondaires comprenaient le nombre de tentatives d’intubation, le dispositif utilisé pour parvenir au succès de la prise en charge des voies aériennes et le respect des protocoles concernant les équipements de protection individuelle (EPI). RéSULTATS: Au cours de la période à l’étude, 123 patients ont été évalués pour une prise en charge des voies aériennes, et 117 patients ont bénéficié d’interventions au niveau des voies aériennes réalisées par l’ERIT. Le taux de réussite de la première tentative d’intubation était de 92 %, et un vidéolaryngoscope a été le dispositif menant à une intubation réussie dans 93 % des interventions. Des épisodes d’hypoxémie (saturation périphérique en oxygène [SpO
- Published
- 2021
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6. Clinical Considerations for Implanted Neurological Devices in Patients Undergoing Hyperbaric Oxygen Therapy: A Case Report and Review of Manufacturer Guidelines
- Author
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Simone Schiavo, Connor T. A. Brenna, Anuj Bhatia, William J. Middleton, and Rita Katznelson
- Subjects
Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health - Abstract
Patients with implanted medical devices are increasingly referred for hyperbaric oxygen therapy (HBOT), and the safety of exposing some of these devices to hyperbaric environments has not previously been explored. There is a paucity of evidence surrounding the management of implanted neurological devices such as neurostimulators and intrathecal drug delivery (IDD) pumps in the context of HBOT. However, these devices can be expected to harbor unique risks; for example, vacant space in the reservoir of an implanted IDD pump may change in pressure and volume during the compression and decompression phases of HBOT, resulting in a damaged or dysfunctional device. We present the case of a 27-year-old woman with cerebral palsy referred for HBOT to manage a necrotizing soft tissue infection cultured from a dehiscent abdominal wound at the previous implantation site of an intrathecal baclofen pump. An HBOT protocol was ultimately chosen in partnership with the patient and her family, but treatment was not performed due to a paucity of evidence that the implanted IDD pump could safely withstand hyperbaric exposure. In this review, we have synthesized manufacturer recommendations regarding the management of implanted neurological devices before, during, and after HBOT to inform future decision-making in this setting. Among these recommendations, we highlight that neurostimulators should be switched off for the duration of HBOT and implanted pumps should be refilled prior to each treatment session to minimize empty reservoir space.
- Published
- 2023
- Full Text
- View/download PDF
7. Mechanistic Rationale and Clinical Efficacy of Hyperbaric Oxygen Therapy in Chronic Neuropathic Pain: An Evidence-Based Narrative Review
- Author
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Julian DeBacker, Rita Katznelson, Simone Schiavo, Anuj Bhatia, Marina Englesakis, and Carine Djaiani
- Subjects
Medicine (General) ,medicine.medical_specialty ,Evidence-based practice ,Analgesic ,Population ,MEDLINE ,Review Article ,R5-920 ,Trigeminal neuralgia ,medicine ,Humans ,Intensive care medicine ,education ,Hyperbaric Oxygenation ,education.field_of_study ,business.industry ,Postherpetic neuralgia ,Chronic pain ,medicine.disease ,Treatment Outcome ,Anesthesiology and Pain Medicine ,Neurology ,Neuropathic pain ,Neuralgia ,Chronic Pain ,business - Abstract
Background. Chronic neuropathic pain is a condition affecting an increasing proportion of the general population and its management requires a comprehensive, multidisciplinary program. A growing body of evidence supports the use of hyperbaric oxygen therapy (HBOT) in several chronic neuropathic pain conditions; however, its role and efficacy remain unclear. Purpose. To summarize current evidence for the mechanistic rationale of HBOT in chronic neuropathic pain conditions and to evaluate its clinical efficacy. Methods. This narrative review was conducted after searching the following databases (Medline, Embase, Cochrane, PsycINFO, the Web of Science, Scopus, ClinicalTrials. gov, WHO ICTRP, and ProQuest Digital Dissertation) from January 1946 to March 2020. Articles published in English that involved either animal or human studies with acute or chronic neuropathic pain evaluating any HBOT-related intervention were included. Results. A total of 2971 citations were identified. A total of 29 studies were included in this review. The mechanisms of action for HBOT use in neuropathic conditions included the primary effects of hyperoxia and edema resolution, as well as the secondary effects pertinent to the production of oxygen and nitrogen reactive species (serving as pain signaling molecules), nitric oxide-dependent release of opioid peptides, and reduction of inflammatory mediators. A robust evidence for HBOT use in the clinical setting was associated with chronic regional pain syndrome and chronic primary bladder pain syndrome. Some evidence supported its use for chronic secondary (peripheral) neuropathic pain including radiation-induced plexus neuropathies, postherpetic neuralgia, and trigeminal neuralgia. Conclusions. HBOT has been shown to have antinociceptive and analgesic effects in animal models of inflammatory, neuropathic, and chronic pain. Human studies demonstrated beneficial effects of HBOT in improving clinical outcomes such as pain scores, pain-related symptoms, and quality of life. A systematic methodology of HBOT application is necessary to confirm its safety and efficacy.
- Published
- 2021
- Full Text
- View/download PDF
8. Effect of intravenous acetaminophen on postoperative outcomes in hip fracture patients: a systematic review and narrative synthesis
- Author
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Jenny Sue Hyun Cho, Kristian McCarthy, Simone Schiavo, Binu Jacob, Marina Engelsakis, Michael Zywiel, Keyvan Karkouti, Stuart McCluskey, Hance Clarke, and Jean Wong
- Subjects
Analgesics, Opioid ,Pain, Postoperative ,Anesthesiology and Pain Medicine ,Hip Fractures ,Quality of Life ,Humans ,General Medicine ,Prospective Studies ,Analgesics, Non-Narcotic ,Acetaminophen ,Aged ,Retrospective Studies - Abstract
Hip fractures are debilitating in older adults because of their impact on quality of life. Opioids are associated with adverse effects in this population, so oral acetaminophen is commonly prescribed to minimize opioid use. Intravenous (iv) acetaminophen has been reported to have superior efficacy and bioavailability than oral acetaminophen. Nevertheless, its effect on postoperative outcomes in emergency hip fractures is unclear. This systematic review assessed the effect of iv acetaminophen on postoperative outcomes in older hip fracture patients.We searched multiple databases from inception to June 2021 for studies on adults50 yr of age undergoing emergency hip fracture surgery who received iv acetaminophen (or paracetamol) and that reported postoperative outcomes. Relevant titles, abstracts, and full texts were screened based on the eligibility criteria. The Newcastle-Ottawa scale was used to assess the quality of the selected papers.Of 3,510 initial studies, four met the inclusion criteria. One was a prospective cohort study and three were retrospective cohort studies. All four studies used historical control groups. Three studies reported a significantly lower mean opioid dose with iv acetaminophen than with oral acetaminophen. Three studies also reported a significantly shorter hospital stay. One study each reported a significant decrease in the number of missed physical therapy sessions, the need for one-to-one supervision, and episodes of delirium.There is very limited low-level evidence that iv acetaminophen improves preoperative and postoperative analgesia and shortens hospital stay in older hip fracture patients. Nevertheless, our results should be interpreted with caution since there are no prospective randomized trials investigating whether iv acetaminophen improves postoperative outcomes in this patient population.PROSPERO (CRD42021198174); registered 15 August 2021.RéSUMé: OBJECTIF: Les fractures de la hanche sont débilitantes chez les personnes âgées en raison de leur impact sur leur qualité de vie. Les opioïdes sont associés à des effets indésirables chez cette population, de sorte que l’acétaminophène par voie orale est couramment prescrit pour minimiser la consommation d’opioïdes. L’acétaminophène par voie intraveineuse (IV) a une efficacité et une biodisponibilité supérieures à celles de l’acétaminophène par voie orale. Néanmoins, son effet sur les devenirs postopératoires dans les fractures d’urgence de la hanche n’est pas clair. Cette revue systématique a évalué l’effet de l’acétaminophène IV sur les devenirs postopératoires chez les patients âgés avec une fracture de la hanche.Nous avons effectué des recherches dans plusieurs bases de données de leur création à juin 2021 pour en tirer les études portant sur des adultes50 ans bénéficiant d’une chirurgie d’urgence pour une fracture de la hanche et ayant reçu de l’acétaminophène IV (ou paracétamol), et qui rapportait les devenirs postopératoires. Les titres, résumés et textes intégraux pertinents ont été sélectionnés en fonction des critères d’admissibilité. L’échelle de Newcastle-Ottawa a été utilisée pour évaluer la qualité des articles sélectionnés.Sur les 3510 études initiales, quatre ont répondu aux critères d’inclusion. L’une était une étude de cohorte prospective et trois étaient des études de cohorte rétrospectives. Les quatre études ont utilisé des groupes témoins historiques. Trois études ont rapporté une dose moyenne d’opioïdes significativement plus faible avec l’acétaminophène IV qu’avec de l’acétaminophène par voie orale. Trois études ont également rapporté un séjour à l’hôpital significativement plus court. Une diminution significative du nombre de séances de physiothérapie manquées a été rapporté dans une étude, une autre a rapporté une diminution significative de la nécessité de supervision individuelle, et une troisième une réduction des épisodes d’état confusionnel aigu.: Il n’existe que très peu de données probantes qui sont de faible qualité et selon lesquelles l’acétaminophène IV améliore l’analgésie préopératoire et postopératoire et réduit la durée de séjour à l’hôpital chez les patients âgés atteints d’une fracture de hanche. Néanmoins, nos résultats doivent être interprétés avec prudence car il n’existe pas d’étude randomisée prospective évaluant si l’acétaminophène IV améliore les issues postopératoires dans cette population de patients. ENREGISTREMENT DE L’éTUDE: PROSPERO (CRD42021198174); enregistrée le 15 août 2021.
- Published
- 2021
9. Magnitude and Clinical Predictors of Blood Pressure Changes in Patients Undergoing Hyperbaric Oxygen Therapy: A Retrospective Study
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Julian DeBacker, Rita Katznelson, Daniel Santa Mina, Simone Schiavo, Lisa Albertini, Carine Djaiani, Marcus Vinicius De Moraes, Mohammad Kanj, and Stephanie Buryk-Iggers
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Adult ,Male ,medicine.medical_specialty ,hemodynamic change ,Future studies ,hypertension ,genetic structures ,Health, Toxicology and Mutagenesis ,030232 urology & nephrology ,Hemodynamics ,lcsh:Medicine ,Angiotensin-Converting Enzyme Inhibitors ,030204 cardiovascular system & hematology ,Article ,03 medical and health sciences ,Angiotensin Receptor Antagonists ,0302 clinical medicine ,Hyperbaric oxygen ,Internal medicine ,medicine ,Humans ,In patient ,Longitudinal Studies ,Risk factor ,Longitudinal cohort ,Aged ,Retrospective Studies ,Hyperbaric Oxygenation ,business.industry ,lcsh:R ,Public Health, Environmental and Occupational Health ,blood pressure ,Retrospective cohort study ,Middle Aged ,hyperbaric oxygen therapy ,Blood pressure ,Cardiology ,Female ,business - Abstract
Hyperbaric oxygen therapy (HBOT) is widely used to treat several pathologies. The hemodynamic changes during HBOT, particularly the magnitude of arterial blood pressure (ABP) increase, are not completely understood. No clinical predictors for HBOT-induced ABP increase have been described. The purpose of this study was to quantify ABP changes in patients undergoing HBOT and to examine their predictors. This retrospective longitudinal cohort study examined 3291 elective HBOT sessions. Non-invasive ABP was recorded before and after each session. The primary outcome was to quantify the HBOT-induced ABP rise. The secondary outcome was to determine the ABP-rise predictors among demographic and clinical variables. Overall, ABP increased significantly after HBOT, this finding was more evident in the hypertensive subgroup compared to the normotensive one (+6 vs. +16.2 mmHg). Clinical predictors of significant post-HBOT ABP change were history of hypertension and pre-session baseline ABP classification. This study demonstrates an absolute HBOT-induced ABP rise. This change is clinically relevant in patients with history of hypertension. A higher baseline ABP seems a risk factor for clinically relevant ABP change. Pre-session ABP should be used clinically as an indicator for strict ABP monitoring during HBOT, future studies are recommended to explore the ABP optimization before starting an HBO treatment.
- Published
- 2020
10. Hyperbaric oxygen and focused rehabilitation program: a feasibility study in improving upper limb motor function after stroke
- Author
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Carine Djaiani, D. Santa Mina, D Richardson, Rita Katznelson, Hance Clarke, J Uehling, Jo Carroll, Simone Schiavo, Stephanie Buryk-Iggers, and M Gershinsky
- Subjects
Male ,medicine.medical_specialty ,Physiology ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Motor function ,Upper Extremity ,03 medical and health sciences ,0302 clinical medicine ,Physical medicine and rehabilitation ,Hyperbaric oxygen ,Physiology (medical) ,Neuroplasticity ,medicine ,Humans ,Single-Blind Method ,030212 general & internal medicine ,Prospective Studies ,Stroke ,Aged ,Hyperbaric Oxygenation ,Nutrition and Dietetics ,Rehabilitation ,business.industry ,Stroke Rehabilitation ,Exercise therapy ,General Medicine ,Recovery of Function ,Middle Aged ,medicine.disease ,Exercise Therapy ,medicine.anatomical_structure ,Imagination ,Upper limb ,Feasibility Studies ,Female ,business ,030217 neurology & neurosurgery - Abstract
Neuroplasticity and recovery after stroke can be enhanced by a rehabilitation program pertinent to upper limb motor function exercise and mental imagery (EMI) as well as hyperbaric oxygen therapy (HBOT). We assessed feasibility and safety of the combined approach utilizing both HBOT and EMI, and to derive preliminary estimates of its efficacy. In this randomized controlled trial, 27 patients with upper extremity hemiparesis at 3–48 months after stroke were randomized to receive either a complementary rehabilitation program of HBOT-EMI (intervention group), or EMI alone (control group). Feasibility and safety were assessed as total session attendance, duration of sessions, attrition rates, missing data, and intervention-related adverse events. Secondary clinical outcomes were assessed with both objective tools and self-reported measures at baseline, 8 weeks (end of treatment), and 12-weeks follow-up. Session attendance, duration, and attrition rate did not differ between the groups; there were no serious adverse events. Compared with baseline, there were significant sustained improvements of objective and subjective outcomes’ measures in the intervention group, and a single improvement in an objective measure in the control group. Between-group outcome comparisons were not statistically significant. This study demonstrated that the combination HBOT-EMI was a safe and feasible approach in patients recovering from chronic stroke. There were also trends for improved motor function of the affected upper limb after the treatments. ClinicalTrials.gov registration no.: NCT02666469. Novelty HBOT combined with an upper limb exercise and mental imagery rehabilitation program is feasible and safe in chronic stroke patients. This combined approach showed trends for improved functional recovery.
- Published
- 2020
11. The Treatment of Perioperative Spinal Cord Injury With Hyperbaric Oxygen Therapy: A Case Report
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Eric M. Massicotte, Marcus V De Moraes, Simone Schiavo, William J Middleton, Jamie R F Wilson, and Rita Katznelson
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Male ,Weakness ,Decompression ,03 medical and health sciences ,0302 clinical medicine ,Hyperbaric oxygen ,Postoperative Complications ,medicine ,Humans ,Orthopedics and Sports Medicine ,Spinal cord injury ,Spinal Cord Injuries ,Aged ,030222 orthopedics ,Hyperbaric Oxygenation ,business.industry ,Laminectomy ,Perioperative ,medicine.disease ,Decompression, Surgical ,Riluzole ,Spinal Fusion ,Treatment Outcome ,Anesthesia ,Radiological weapon ,Ambulatory ,Neurology (clinical) ,medicine.symptom ,business ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Study design Case report (level IV evidence). Objective To describe a potential novel application of hyperbaric oxygen therapy (HBOT) in the successful treatment of a postoperative spinal cord injury. Summary of background data A 68-year-old man presented with an acute spinal cord injury (ASIA impairment scale D), on the background of degenerative lower thoracic and lumbar canal stenosis. He underwent emergent decompression and instrumented fusion (T9-L5), with an uncomplicated intraoperative course and no electrophysiological changes. Immediate postoperative assessment demonstrated profound bilateral limb weakness (1/5 on the Medical Research Council [MRC] grading scale, ASIA impairment scale B), without radiological abnormality. Methods Conventional medical management (hypertension, level 2 care) was instigated with the addition of Riluzole, with no effect after 30 hours. At 36 hours 100% oxygen at 2.8 atmospheres was applied for 90 minutes, and repeated after 8 hours, with a further three treatments over 48 hours. Results The patient demonstrated near-immediate improvement in lower limb function to anti-gravity (MRC grading 3/5) after one treatment. Motor improvement continued over the following treatments, and after 2 weeks the patient was ambulatory. At 4 months, the patient demonstrated normal motor function with no sphincteric disturbance. Conclusion The application of HBOT contributed to the immediate and sustained improvement (ASIA B to ASIA E) in motor recovery after postoperative spinal cord injury. HBOT may represent a new avenue of therapy for spinal cord injury, and requires further prospective investigation. Level of evidence 4.
- Published
- 2020
12. GIFTEDNESS: A SURVEY ABOUT BRAZILIAN CHILDREN AND EDUCATOR'S (MIS) CONCEPTION / SUPERDOTACIÓN: INVESTIGACIÓN SOBRE NIÑOS BRASILEÑOS Y LA CONCEPCIÓN (ERRÓNEA) DE EDUCADORES
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Taís Crema Remoli Ferreira, Vera Lúcia Messias Fialho Capellini, Eliana Marques Zanata, Elana Simone Schiavo Caramano, Laís Mayssi Rodrigues, and Regiani Aparecida Zacarias
- Abstract
This study aimed to identify the number of gifted children, and the teachers’ (mis) conception about giftedness. The study was carried out in public schools in the city of Bauru in the state of São Paulo, Brazil. Data was collected by asking teachers to fill in a standardized form about the characteristics of gifted children and to answer a questionnaire agreeing or disagreeing with assumptions regarding gifted students’ behavior. The research was carried out with 111 teachers from elementary school: 82% with a major degree in pedagogy and 22.5% with a post-graduation (lato sensu) course. This research revealed important and relevant data that may be used as guidelines to plan teacher development courses and seminar on the subject, including that 29% of the teachers have little knowledge on the subject; 36,9% teacher were currently teaching gifted students and 93,6% of them had students with three or more characteristics of giftedness in their classes. Gifted children were mostly reported in Arts (19.82%), secondly in logical reasoning (5.41%), and thirdly in intelligence (4.5%). Este estudio tuvo como objetivo identificar la cantidad de niños superdotados y la concepción (errónea) de los maestros sobre la superdotación. El estudio se realizó en escuelas públicas de la ciudad de Bauru en el estado de Sao Paulo, Brasil. La fecha se recopiló solicitando a los maestros que rellenen un formulario estandarizado sobre las características de los niños superdotados y que respondan a un cuestionario de acuerdo o en desacuerdo con los supuestos sobre el comportamiento de los alumnos superdotados. La investigación se realizó con 111 docentes de la escuela primaria: 82% con especialización en pedagogía y 22.5% con un curso de posgrado (lato sensu). Esta investigación reveló datos importantes y relevantes que pueden usarse como pautas para planificar cursos y seminarios de desarrollo docente sobre el tema, incluido que el 29% de los maestros tienen poco conocimiento sobre el tema; el 36.9% de los maestros actualmente enseñaban a estudiantes superdotados y el 93.6% de ellos tenían estudiantes con tres o más características de superdotados en sus clases. Los niños superdotados se informaron principalmente en Artes (19.82%), en segundo lugar en razonamiento lógico (5.41%) y en tercer lugar en inteligencia (4.5%).
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- 2019
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13. Arterial Blood Gas Analysis in Breath-Hold Divers at Depth
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Simone Schiavo, Alex Rizzato, Matteo Paganini, Richard E. Moon, Ennio Talamonti, Enrico M. Camporesi, Gerardo Bosco, Giacomo Garetto, and Luca Martani
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Arterial blood gas ,Blood gas analysis ,Breath-hold diving ,Physiology ,Underwater ,underwater ,030204 cardiovascular system & hematology ,lcsh:Physiology ,Hypoxemia ,03 medical and health sciences ,0302 clinical medicine ,breath-hold diving ,Physiology (medical) ,medicine.artery ,Hyperventilation ,medicine ,Radial artery ,Original Research ,Hyperoxia ,lcsh:QP1-981 ,business.industry ,030229 sport sciences ,Partial pressure ,arterial blood gas ,blood gas analysis ,Anesthesia ,physiology ,Breathing ,Arterial blood ,medicine.symptom ,business ,Hypercapnia ,human activities - Abstract
The present study aimed to evaluate the partial pressure of arterial blood gases on breath-hold divers performing a submersion at 40 m. Eight breath-hold divers were enrolled for the trials held at “Y-40 THE DEEP JOY” pool (Montegrotto Terme, Padova, Italy). Prior to submersion, an arterial cannula in the radial artery of the non-dominant limb was positioned. All divers performed a sled-assisted breath-hold dive to 40 meters. Three blood samplings occurred: at 10 minutes prior to submersion, at 40 meters depth, and within 2 minutes after diver’s surfacing and after resuming normal ventilation. Blood samples were analyzed immediately on site. Six subjects completed the experiment, without diving-related problems. The theoretically predicted hyperoxia at the bottom was observed in 4 divers out of 6, while the other 2 experienced a reduction in the partial pressure of oxygen (paO2) at the bottom. There were no significant increases in arterial partial pressure of carbon dioxide (paCO2) at the end of descent in 4 of 6 divers, while in 2 divers paCO2 decreased. Arterial mean pH and mean bicarbonate (HCO3-) levels exhibited minor changes. There was a statistically significant increase in mean arterial lactate level after the exercise. Ours was the first attempt to verify real changes in blood gases at a depth of 40 m during a breath-hold descent in free-divers. We demonstrated that, at depth, relative hypoxemia can occur, presumably caused by lung compression. Also, hypercapnia exists at depth, to a lesser degree than would be expected from calculations, presumably because of pre-dive hyperventilation and carbon dioxide distribution in blood and tissues.
- Published
- 2018
- Full Text
- View/download PDF
14. Proposal of video classes as previous organizers for a meaninful learning in English teaching at EJA
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Caramano, Elana Simone Schiavo [UNESP], Universidade Estadual Paulista (Unesp), and Zanata, Eliana Marques [UNESP]
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Educação de jovens e adultos ,Videoaulas ,Aprendizagem significativa ,Língua estrangeira moderna – Inglês - Abstract
Submitted by Elana Simone Schiavo Caramano null (lanacaramano@gmail.com) on 2017-03-21T12:24:57Z No. of bitstreams: 1 Dissertação_MESTRADO_Docencia_ElanaCaramano2017.pdf: 6428227 bytes, checksum: 25b42f3c8b34b4e9f22db1ae1d16e337 (MD5) Approved for entry into archive by Juliano Benedito Ferreira (julianoferreira@reitoria.unesp.br) on 2017-03-23T18:28:10Z (GMT) No. of bitstreams: 1 caramano_ess_me_bauru.pdf: 6428227 bytes, checksum: 25b42f3c8b34b4e9f22db1ae1d16e337 (MD5) Made available in DSpace on 2017-03-23T18:28:10Z (GMT). No. of bitstreams: 1 caramano_ess_me_bauru.pdf: 6428227 bytes, checksum: 25b42f3c8b34b4e9f22db1ae1d16e337 (MD5) Previous issue date: 2017-02-24 Parece não ser mais possível pensar na Educação de Jovens e Adultos sem contemplar o uso das Tecnologias Digitais de Informação e Comunicação (TDIC), uma área aberta à exploração para a criação de novas e efetivas situações de ensino e aprendizagem. Tendo em vista o atendimento a um público tão heterogêneo quanto o do CEEJA, em sua modalidade semipresencial, objetiva-se, neste trabalho, apresentar uma proposta de elaboração de videoaulas instrucionais como organizadores prévios para uma aprendizagem mais significativa na disciplina de língua estrangeira moderna – LEM – Inglês. Essa proposta representa a inserção das TDICs, especificamente, na implementação de um ambiente digital de aprendizagem, na mediação contextual do material didático (EJA – Mundo do Trabalho), diante das dificuldades percebidas em observações em sala de aula (de um CEEJA no interior paulista) e dos inúmeros fatores dificultadores que os educandos da EJA enfrentam ao retomarem os estudos. Daí a pertinência de uma reflexão sobre a prática pedagógica em suas relações históricas para o desenvolvimento de novas estratégias perante as demandas e premências de um mundo em constante mudança. Assim, considerando o princípio da filosofia freireana no que concerne ao respeito dos saberes dos educandos, bem como os conceitos de Ausubel sobre aprendizagem significativa em relação àquilo que o aprendiz já sabe, dá-se ênfase à aprendizagem como processo dialógico de significação e autonomia que compreende a aquisição de um repertório de signos, na articulação de múltiplas linguagens (verbais, não verbais, audíveis), por meio de videoaulas para a constituição da consciência e autonomia do educando. Ao educador cabe o papel de mediador desse processo, com a consciência de suas ações como construtor de conhecimentos, atento às necessidades dos educandos e reflexivo sobre suas práticas pedagógicas. It seems no longer possible to think of the Youth and Adult Education without contemplating the use of Digital Information and Communication Technologies (DICT), an area where new teaching-learning situations can be explored to provide new and effective situations of teaching and learning. Bearing in mind the importance of assisting such a diverse audience as the students at a CEEJA, a Youth and Adult Education program in Brazil, in semi-distance learning mode, the objective of this dissertation is to develop instructional video lessons as previous organizers for a more meaningful learning of the discipline English as a foreign language, and the insertion of DICT through the implementation of a digital learning environment, on contextual mediation of learning material (EJA -Mundo do Trabalho - EJA World of Work), in face of the difficulties detected by observations in the classroom (at a CEEJA in São Paulo state) and for numerous complicating factors that students from EJA face when they resume their studies. Hence the relevance of a reflection on teaching practice in its historical relationship to the development of new strategies to meet the demands and exigencies of a world that is constantly changing. Therefore, considering the principle of Freire's philosophy regarding the respect for knowledge as well as the concepts of Ausubel about meaningful learning with respect to the learner already knows, this dissertation focuses on learning as a dialogic process of signification and autonomy, which includes the acquisition of a repertoire of signs, the articulation of multiple languages (verbal, nonverbal, audible), through video classes for the development of consciousness and autonomy of the learners. The educator’s role is being a mediator of this process, being aware of actions as a builder of knowledge, watching out the needs of learners and being reflective about their own teaching practices.
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- 2017
15. A Small Sample of Physical Accessibility in Some Schools in Sao Paulo – Brazil
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Veronica Rodrigues Mangili, Vera Lúcia Messias Fialho Capellini, Elana Simone Schiavo Caramano, and Maria Carolina C. Gamba-Yoshida
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Protocol (science) ,Medical education ,Engineering ,business.industry ,Pedagogy ,Continuous education ,Legislation ,Small sample ,Special education ,business ,Physical accessibility - Abstract
This study deals with the continuous education of teachers in special education, offered by the platform of Sao Paulo Network Program of teacher’s formation (REDEFOR), as well as the evaluation of the results of a study conducted by 38 in-course teachers regarding accessibility of 29 schools in the State of Sao Paulo. The objective, therefore, is to describe the results of the data collected by the in-course teachers about the accessibility conditions in their schools, through the protocol of routes. Evaluating 870 items, 19% were considered accessible, 18% were inaccessible, and 68% were not found adapted elements or accessible. It should be noted, as well, that a high percentage of elements not found, especially the fundamental as ramps (security), signs (communication) and elevators. Considering that the concomitance of accessible and inaccessible elements at the same amount where adapting an environmental requirement in rush in order to obey the legislation.
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- 2016
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16. Post-traumatic stress symptoms in mothers of children with leukaemia undergoing the first 12 months of therapy: Predictive models
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Simone Schiavo, Sabrina Bonichini, Marta Tremolada, and Marta Pillon
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Adult ,Male ,Pediatrics ,medicine.medical_specialty ,Time Factors ,Adolescent ,Psychological intervention ,Mothers ,PEDIATRIC CANCER SURVIVORS ,Models, Psychological ,Medical care ,Stress Disorders, Post-Traumatic ,Psychological health ,DISTRESS ,PARENTS ,Paediatric cancer ,QUALITY-OF-LIFE ,Adaptation, Psychological ,Humans ,Medicine ,MEDICAL TRAUMATIC STRESS ,Child ,Applied Psychology ,Leukemia ,CHILDHOOD-CANCER ,business.industry ,Public Health, Environmental and Occupational Health ,Traumatic stress ,Infant ,General Medicine ,General Chemistry ,Checklist ,Child, Preschool ,QUALITY-OF-LIFE, PEDIATRIC CANCER SURVIVORS, MEDICAL TRAUMATIC STRESS, CHILDHOOD-CANCER, PSYCHOLOGICAL ADJUSTMENT, SOCIAL SUPPORT, PARENTS, DISTRESS, SYMPTOMATOLOGY, FATHERS ,SYMPTOMATOLOGY ,Female ,PSYCHOLOGICAL ADJUSTMENT ,SOCIAL SUPPORT ,FATHERS ,business ,Stress, Psychological ,Follow-Up Studies ,Clinical psychology - Abstract
The aim of this study consists in the measurement of psychological health and adaptation in mothers of children during the first 12 months of treatment for leukaemia and in the identification of possible early predictors. Ninety-four mothers were followed longitudinally at one week (T1), one month (T2), six months (T3) and 12 months (T4) post-diagnosis. The instruments used were: PTSD symptom checklist, BSI-18, Problem Scale, Ladder of life and an in-depth interview (EFI-C). Couple connectedness, family routine reorganisation, parental communication around the child's illness and trust in the medical care significantly increased from T1 to T4. Two models are proposed concerning possible predictors of mothers' PTSS at T2 and at T3. Clinical suggestions are proposed on the basis of our empirical findings in order to plan informative, clinical and practical interventions for mothers of children under treatment for leukaemia.
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- 2012
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17. Post-traumatic stress symptoms among mothers of children with leukemia undergoing treatment: a longitudinal study
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Donatella Aloisio, Sabrina Bonichini, Marta Pillon, Simone Schiavo, Marta Tremolada, and Modesto Carli
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Longitudinal study ,Pediatrics ,medicine.medical_specialty ,Brief Symptom Inventory 18 ,business.industry ,Psychological intervention ,Traumatic stress ,Experimental and Cognitive Psychology ,Checklist ,Psychiatry and Mental health ,Oncology ,medicine ,Anxiety ,Cognitive skill ,medicine.symptom ,business ,Depression (differential diagnoses) - Abstract
Objective To assess post-traumatic stress symptoms (PTSS) in mothers of children over 2 years of leukemia treatment, to identify possible early family and child predictors of this symptomatology and to indicate the temporal trajectory of PTSS. Methods Participants were 76 Italian mothers (mean age = 37.30 years; SD = 6.07) of children receiving treatment for acute lymphoblastic (n = 69) or myeloid (n = 7) leukemia. Mothers had 12.05 years of education (SD = 3.87), and their incomes were average (52.1%), high (26%) and low (21.9%) for Italian norms, never in poverty. The pediatric patients with leukemia were equally distributed by gender with their mean age of 7.10 years (SD = 4.18). Post-traumatic stress symptoms were measured by a 17-item checklist. Scales assessing anxiety, depression, physical (Brief Symptom Inventory 18) and cognitive functioning (Problem Scale), and life evaluation were also used. There were five assessment points: 1 week (T1), 1 month (T2), 6 months (T3), 12 months (T4) and 24 months post-diagnosis (T5). Results The main results indicated moderate presence of clinical PTSS (≥9 symptoms: 24% at T2, 18% at T3, 16% at T4 and 19% at T5) that remained stable across time points, whereas Brief Symptom Inventory 18 Global score decreased and life evaluation improved. A series of hierarchical regression models identified cognitive functioning early after the diagnosis as the best predictive factor of PTSS across time points. Conclusion Specific psychological interventions could be devised for mothers at risk for short and long-term PTSS just after the diagnosis. Copyright © 2012 John Wiley & Sons, Ltd.
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- 2012
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18. A Small Sample of Physical Accessibility in Some Schools in Sao Paulo – Brazil
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Capellini, Vera Lucia Messias Fialho, primary, Caramano, Elana Simone Schiavo, additional, Gamba-Yoshida, Maria Carolina C., additional, and Mangili, Veronica Rodrigues, additional
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- 2016
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19. Parental Narratives of Children with Leukemia in the Second Week after the Diagnosis: The Ecocultural Family Interview-Cancer
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Marta Tremolada, Marta Pillon, Sabrina Bonichini, Thomas S. Weisner, and Simone Schiavo
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Health related quality of life ,medicine.medical_specialty ,In depth interviews ,business.industry ,Family medicine ,Medicine ,Cancer ,Narrative ,business ,medicine.disease - Published
- 2013
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20. Posttraumatic stress symptoms among mothers of children with leukemia undergoing treatment: a longitudinal study
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Marta, Tremolada, Sabrina, Bonichini, Donatella, Aloisio, Simone, Schiavo, Modesto, Carli, and Marta, Pillon
- Subjects
Male ,DISORDER ,Adolescent ,Mothers ,ADULT SURVIVORS ,DIAGNOSIS ,FAMILIES ,Life Change Events ,Stress Disorders, Post-Traumatic ,DISTRESS ,PARENTS ,Surveys and Questionnaires ,Adaptation, Psychological ,Humans ,Longitudinal Studies ,Child ,PREDICTORS ,Post-traumatic stress symptoms ,CHILDHOOD-CANCER ,Depression ,leukemia ,ADOLESCENT SURVIVORS ,pediatric ,Italy ,Socioeconomic Factors ,Child, Preschool ,ADOLESCENT SURVIVORS, ADULT SURVIVORS, PARENTS, DISORDER, DISTRESS, PREDICTORS, DIAGNOSIS, FAMILIES ,Regression Analysis ,Female ,Stress, Psychological - Abstract
To assess post-traumatic stress symptoms (PTSS) in mothers of children over 2 years of leukemia treatment, to identify possible early family and child predictors of this symptomatology and to indicate the temporal trajectory of PTSS.Participants were 76 Italian mothers (mean age = 37.30 years; SD = 6.07) of children receiving treatment for acute lymphoblastic (n = 69) or myeloid (n = 7) leukemia. Mothers had 12.05 years of education (SD = 3.87), and their incomes were average (52.1%), high (26%) and low (21.9%) for Italian norms, never in poverty. The pediatric patients with leukemia were equally distributed by gender with their mean age of 7.10 years (SD = 4.18). Post-traumatic stress symptoms were measured by a 17-item checklist. Scales assessing anxiety, depression, physical (Brief Symptom Inventory 18) and cognitive functioning (Problem Scale), and life evaluation were also used. There were five assessment points: 1 week (T1), 1 month (T2), 6 months (T3), 12 months (T4) and 24 months post-diagnosis (T5).The main results indicated moderate presence of clinical PTSS (≥9 symptoms: 24% at T2, 18% at T3, 16% at T4 and 19% at T5) that remained stable across time points, whereas Brief Symptom Inventory 18 Global score decreased and life evaluation improved. A series of hierarchical regression models identified cognitive functioning early after the diagnosis as the best predictive factor of PTSS across time points.Specific psychological interventions could be devised for mothers at risk for short and long-term PTSS just after the diagnosis.
- Published
- 2013
21. Eliciting adaptive emotion in conversations with parents of children receiving therapy for leukemia
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Modesto Carli, Sabrina Bonichini, Marta Tremolada, Marta Pillon, and Simone Schiavo
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Adult ,Male ,Parents ,Interview ,media_common.quotation_subject ,Emotions ,Mothers ,Developmental psychology ,Fathers ,Sex Factors ,Professional-Family Relations ,hemic and lymphatic diseases ,Surveys and Questionnaires ,Adaptation, Psychological ,Humans ,Emotional expression ,Child ,Applied Psychology ,Qualitative Research ,media_common ,Leukemia ,Politeness ,Communication ,Social environment ,Videotape Recording ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,Social relation ,Psychiatry and Mental health ,Leukemia, Myeloid, Acute ,Conversation analysis ,Mood ,Oncology ,Female ,Psychology ,Stress, Psychological ,Qualitative research - Abstract
Clinician-parent communication may often be difficult, especially soon after the diagnosis. The aims of this article are to identify the communication strategies associated with expressions of adaptive emotions in parents and to explore the effect of the type of leukemia and of parent's gender on parents' expressions of emotions. The data are obtained from 4.622 conversational turns of 20 videotaped interviews with 10 mothers and 10 fathers of children at their first hospitalization for leukemia. A coding scheme for parent emotional expressions was reliably applied by two independent judges. An original self-report questionnaire on parents' emotional states was used before and after the interview. Positive politeness of interviewer elicits adaptive emotional expressions in parents. Mothers of children with acute myeloid leukemia and fathers of children with acute lymphoblastic leukaemia appear more distressed during the interview. This interview can be identified as an innovative technique of communication with parents of children with cancer.
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- 2011
22. Investigations of the relics and altar materials relating to the apostles St James and St Philip at the Basilica dei Santi XII Apostoli in Rome
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Kaare Lund Rasmussen, Johannes van der Plicht, Jacopo La Nasa, Erika Ribercini, Maria Perla Colombini, Thomas Delbey, Lilian Skytte, Simone Schiavone, Ulla Kjær, Poul Grinder-Hansen, and Lautaro Roig Lanzillotta
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Relics ,St James ,St Philip ,Basilica dei Santi XII Apostoli ,Radiocarbon ,TL ,Fine Arts ,Analytical chemistry ,QD71-142 - Abstract
Abstract Two types of materials were sampled as part of an investigation of the relics of the Holy Catholic Church of the Apostles St Philip and St James in the Basilica dei Santi Apostoli in Rome: bone- and mummy-materials and architectural samples. The analyses encompassed radiocarbon dating, thermoluminescence dating, gas and liquid chromatographic separation with mass spectrometric detection, X-Ray fluorescence, X-Ray diffraction, inductively coupled plasma mass spectrometry, Raman spectroscopy, and Fourier transform infrared spectroscopy. The results show that the samples were subjected to a number of conservational and exhibition-related treatments. The alleged femoral bone of St James was dated between AD 214 and 340 (2σ confidence), which shows that this cannot be the bone of St James. An encrustation found in a canal in the reliquary in the high altar construction showed the presence of heavily oxidized rapeseed oil, which was radiocarbon dated between AD 267 and 539 (2σ confidence), and a ceramic shard also found in the high altar construction was TL-dated to AD 314–746 (2σ confidence). The two latter dates are consistent with a translation of the relics following the erection of the church at the time of Pope Pelagius I in AD 556–561.
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- 2021
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23. Trace element distribution in human cortical bone microstructure: the potential for unravelling diet and social status in archaeological bones
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Kaare Lund Rasmussen, George R. Milner, Thomas Delbey, Lilian Skytte, Niels Lynnerup, Jørgen Lange Thomsen, Simone Schiavone, Marielva Torino, Lars Agersnap Larsen, and Jesper Lier Boldsen
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Osteons ,Trace element chemistry ,LA-ICP-MS ,Sr ,Ba ,Cu ,Fine Arts ,Analytical chemistry ,QD71-142 - Abstract
Abstract Variation in the trace element chemistry of cortical bone microstructure is delineated for interred and non-interred human femora. This was done to investigate the range of element concentrations that might occur within single bones, specifically the original laminar bone and later osteons, and its potential for investigating chemical life histories. To do so, femora were chosen from individuals who experienced quite different ways of life over the past two millennia. The distributions of Sr, Ba, Cu, and Pb, mostly in partial (early) and complete (late) osteons, in cross-sections of proximal femora were characterized through Laser Ablation Inductively Coupled Plasma Mass Spectrometry. Absolute calibrations of these data were obtained using solution Inductively Coupled Plasma Mass Spectrometry on adjacent dissolved bulk samples. Chemical life histories were approximated by classifying bone microstructure into four categories: laminar bone and 1st, 2nd, and 3rd generation osteons. This four-part sequence, on average, charts the temporal dimension of an individual’s life. Consistent with recent studies of medieval bones, Sr and Ba are thought to be mainly responsive to diet, presumably related to the consumption of mostly locally produced food, while Cu and Pb do the same for heavy metal exposure often attributable to social status or occupation. No systematic differences in these elements were found between interred and non-interred individuals. The effect of diagenesis on interpretations of life histories based on archaeological bone, therefore, are minimized by plotting element concentrations across cortical bone cross-sections.
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- 2020
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24. Comparison of trace element chemistry in human bones interred in two private chapels attached to Franciscan friaries in Italy and Denmark: an investigation of social stratification in two medieval and post-medieval societies
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Kaare Lund Rasmussen, Thomas Delbey, Paolo d’Imporzano, Lilian Skytte, Simone Schiavone, Marielva Torino, Peter Tarp, and Per Orla Thomsen
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Noble families ,Strontium ,Barium ,Copper ,Lead ,Mercury ,Fine Arts ,Analytical chemistry ,QD71-142 - Abstract
Abstract Individuals buried in two 17th–18th Century private chapels, each attached to a Franciscan Friary—one in Italy the other in Denmark—have been studied and sampled for trace element analysis. This selection of individuals allows a comparison of the trace element inventory of members of noble families against friars and townspeople, as well as a comparison between two very similar situations in Denmark and Italy. The relevance of this study is to see if and how differences in social status, and therefore likely differences in dietary habits, are reflected in the trace element chemistry of the bones. Samples of cortical and trabecular tissues have been procured from a long bone, preferentially the femur. The samples have been thoroughly decontaminated. 87 samples from 69 individuals have been analysed for Ca, Mn, Fe, Cu, Sr, Ba, and Pb by ICP-MS and Hg by CV-AAS. Sex and age at death have been established by anthropological analysis for all members of the two noble families. We find systematic differences between the noble family members and the friars (or townspeople) in both Italy and Denmark. The noble families are in both cases low in Sr and Ba compared to the friars and townspeople, which is interpreted as a dietary signal resulting from higher meat consumption than in the comparative groups. Lead concentrations are found to be higher in the noble family members than in the comparative groups, and the Pb concentration seems to increase with age in the Italian noble family, where both young and middle-aged individuals were investigated. Mercury concentrations are higher in some of the Italian noble family members compared to friars and townspeople; whereas in Denmark it seems that Hg was equally available to the noble family members and the friars alike. This is the first comprehensive and comparative study of post-medieval noble families in Denmark and Italy. The results show that there are distinct similarities in the trace element distribution patterns in the noble family members irrespective of country, which is tentatively suggested to be due to their higher social status.
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- 2020
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25. Platelets induce free and phospholipid-esterified 12-hydroxyeicosatetraenoic acid generation in colon cancer cells by delivering 12-lipoxygenase
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Annalisa Contursi, Simone Schiavone, Melania Dovizio, Christine Hinz, Rosa Fullone, Stefania Tacconelli, Victoria J. Tyrrell, Rosalia Grande, Paola Lanuti, Marco Marchisio, Mirco Zucchelli, Patrizia Ballerini, Angel Lanas, Valerie B. O'Donnell, and Paola Patrignani
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blood platelets ,12-HETE ,12-Lipoxygenase ,extracellular vesicles ,colorectal cancer ,plasma membrane phospholipids ,Biochemistry ,QD415-436 - Abstract
Platelets promote tumor metastasis by inducing promalignant phenotypes in cancer cells and directly contributing to cancer-related thrombotic complications. Platelet-derived extracellular vesicles (EVs) can promote epithelial-mesenchymal transition (EMT) in cancer cells, which confers high-grade malignancy. 12S-hydroxyeicosatetraenoic acid (12-HETE) generated by platelet-type 12-lipoxygenase (12-LOX) is considered a key modulator of cancer metastasis through unknown mechanisms. In platelets, 12-HETE can be esterified into plasma membrane phospholipids (PLs), which drive thrombosis. Using cocultures of human platelets and human colon adenocarcinoma cells (line HT29) and LC-MS/MS, we investigated the impact of platelets on cancer cell biosynthesis of 12S-HETE and its esterification into PLs and whether platelet ability to transfer its molecular cargo might play a role. To this aim, we performed coculture experiments with CFSE[5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester]-loaded platelets. HT29 cells did not generate 12S-HETE or express 12-LOX. However, they acquired the capacity to produce 12S-HETE mainly esterified in plasmalogen phospholipid forms following the uptake of platelet-derived medium-sized EVs (mEVs) expressing 12-LOX. 12-LOX was detected in plasma mEV of patients with adenomas/adenocarcinomas, implying their potential to deliver the protein to cancer cells in vivo. In cancer cells exposed to platelets, endogenous but not exogenous 12S-HETE contributed to changes in EMT gene expression, mitigated by three structurally unrelated 12-LOX inhibitors. In conclusion, we showed that platelets induce the generation of primarily esterified 12-HETE in colon cancer cells following mEV-mediated delivery of 12-LOX. The modification of cancer cell phospholipids by 12-HETE may functionally impact cancer cell biology and represent a novel target for anticancer agent development.
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- 2021
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26. Correction to: Investigations of the relics and altar materials relating to the apostles St James and St Philip at the Basilica dei Santi XII Apostoli in Rome
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Kaare Lund Rasmussen, Johannes van der Plicht, Jacopo La Nasa, Erika Ribechini, Maria Perla Colombini, Thomas Delbey, Lilian Skytte, Simone Schiavone, Ulla Kjær, Poul Grinder-Hansen, and Lautaro Roig Lanzillotta
- Subjects
Fine Arts ,Analytical chemistry ,QD71-142 - Abstract
An amendment to this paper has been published and can be accessed via the original article.
- Published
- 2021
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