9 results on '"Sina E. Dominiak"'
Search Results
2. Publisher Correction: Opposite forms of adaptation in mouse visual cortex are controlled by distinct inhibitory microcircuits
- Author
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Tristan G. Heintz, Antonio J. Hinojosa, Sina E. Dominiak, and Leon Lagnado
- Subjects
Science - Published
- 2022
- Full Text
- View/download PDF
3. Optically Induced Calcium-Dependent Gene Activation and Labeling of Active Neurons Using CaMPARI and Cal-Light
- Author
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Christian Ebner, Julia Ledderose, Timothy A. Zolnik, Sina E. Dominiak, Paul Turko, Athanasia Papoutsi, Panayiota Poirazi, Britta J. Eickholt, Imre Vida, Matthew E. Larkum, and Robert N. S. Sachdev
- Subjects
CaMPARI ,Cal-Light ,photoconversion ,photoactivation ,calcium ,optogenetics ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
The advent of optogenetic methods has made it possible to use endogeneously produced molecules to image and manipulate cellular, subcellular, and synaptic activity. It has also led to the development of photoactivatable calcium-dependent indicators that mark active synapses, neurons, and circuits. Furthermore, calcium-dependent photoactivation can be used to trigger gene expression in active neurons. Here we describe two sets of protocols, one using CaMPARI and a second one using Cal-Light. CaMPARI, a calcium-modulated photoactivatable ratiometric integrator, enables rapid network-wide, tunable, all-optical functional circuit mapping. Cal-Light, a photoactivatable calcium sensor, while slower to respond than CaMPARI, has the capacity to trigger the expression of genes, including effectors, activators, indicators, or other constructs. Here we describe the rationale and provide procedures for using these two calcium-dependent constructs (1) in vitro in dissociated primary neuronal cell cultures (CaMPARI & Cal-Light); (2) in vitro in acute brain slices for circuit mapping (CaMPARI); (3) in vivo for triggering photoconversion or gene expression (CaMPARI & Cal-Light); and finally, (4) for recovering photoconverted neurons post-fixation with immunocytochemistry (CaMPARI). The approaches and protocols we describe are examples of the potential uses of both CaMPARI & Cal-Light. The ability to mark and manipulate neurons that are active during specific epochs of behavior has a vast unexplored experimental potential.
- Published
- 2019
- Full Text
- View/download PDF
4. What moves when mice move a single whisker to touch? Individuality and stereotypy in behavior
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Marcel Staab, Keisuke Sehara, Nora-Laurine Bahr, Sina E. Dominiak, Matthew E. Larkum, and Robert N. S. Sachdev
- Abstract
A key function of the brain is to move the body through a rich, complex environment. When rodents engage their environment, they move their whiskers as they extract tactile information. Even though the study of whisking has a long history, the details of what mice move when they move a whisker actively to touch are still unknown. Here we trained head fixed mice in a simple go-cue task to move a whisker on one side of the face to touch a sensor and tracked facial movements. Our analysis shows that mice specifically control the movement of the whisker they use to touch and that as they move their whiskers, they move their nose and apply forces on the head-post in a manner that reflects the behavioral epoch. Importantly, mice controlled the setpoint, amplitude and frequency of movement of whiskers bilaterally and individually. Additionally, even though mice achieved the goal of the task -- to touch the sensor within 2 seconds -- how they did so, how they coordinated movement of the nose and forces on head post with movement of individual whiskers was stereotyped, specific and related to the distance they needed to move a whisker to touch the sensor. Our work shows how stereotyped mouse behavior can be, and it emphasizes both the level of fine motor control mice can exert over individual whiskers and the extent of facial movements in a goal directed whisking-to-touch task.
- Published
- 2022
5. Efficient training approaches for optimizing behavioral performance and reducing head fixation time
- Author
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Anna Nasr, Sina E. Dominiak, Keisuke Sehara, Mostafa A. Nashaat, Robert N. S. Sachdev, and Matthew E. Larkum
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Mice ,Multidisciplinary ,Behavior, Animal ,Head Movements ,Animals ,Learning - Abstract
The use of head fixation has become routine in systems neuroscience. However, whether the behavior changes with head fixation, whether animals can learn aspects of a task while freely moving and transfer this knowledge to the head fixed condition, has not been examined in much detail. Here, we used a novel floating platform, the “Air-Track”, which simulates free movement in a real-world environment to address the effect of head fixation and developed methods to accelerate training of behavioral tasks for head fixed mice. We trained mice in a Y maze two choice discrimination task. One group was trained while head fixed and compared to a separate group that was pre-trained while freely moving and then trained on the same task while head fixed. Pre-training significantly reduced the time needed to relearn the discrimination task while head fixed. Freely moving and head fixed mice displayed similar behavioral patterns, however, head fixation significantly slowed movement speed. The speed of movement in the head fixed mice depended on the weight of the platform. We conclude that home-cage pre-training improves learning performance of head fixed mice and that while head fixation obviously limits some aspects of movement, the patterns of behavior observed in head fixed and freely moving mice are similar.
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- 2022
6. Coordination between Eye Movement and Whisking in Head-Fixed Mice Navigating a Plus Maze
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Ronny Bergmann, Keisuke Sehara, Sina E. Dominiak, Jens Kremkow, Matthew E. Larkum, and Robert N. S. Sachdev
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Mice ,Eye Movements ,Touch ,Movement ,Vibrissae ,General Neuroscience ,Animals ,General Medicine - Abstract
Navigation through complex environments requires motor planning, motor preparation, and the coordination between multiple sensory–motor modalities. For example, the stepping motion when we walk is coordinated with motion of the torso, arms, head, and eyes. In rodents, movement of the animal through the environment is coordinated with whisking. Even head-fixed mice navigating a plus maze position their whiskers asymmetrically with the bilateral asymmetry signifying the upcoming turn direction. Here we report that, in addition to moving their whiskers, on every trial mice also move their eyes conjugately in the direction of the upcoming turn. Not only do mice move their eyes, but they coordinate saccadic eye movement with the asymmetric positioning of the whiskers. Our analysis shows that asymmetric positioning of whiskers predicted the turn direction that mice will make at an earlier stage than eye movement. Consistent with these results, our observations also revealed that whisker asymmetry increases before saccadic eye movement. Importantly, this work shows that when rodents plan for active behavior, their motor plans can involve both eye and whisker movement. We conclude that, when mice are engaged in and moving through complex real-world environments, their behavioral state can be read out in the movement of both their whiskers and eyes.
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- 2022
7. Predicting behavior from eye movement and whisking asymmetry
- Author
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Kremkow J, Robert N. S. Sachdev, Keisuke Sehara, Bergmann R, Matthew E. Larkum, and Sina E. Dominiak
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Motor planning ,Computer science ,Movement (music) ,business.industry ,Whisking in animals ,media_common.quotation_subject ,Whiskers ,Work (physics) ,Eye movement ,Sensory system ,Torso ,Asymmetry ,Saccadic masking ,medicine.anatomical_structure ,medicine ,Computer vision ,Artificial intelligence ,business ,media_common - Abstract
Navigation through complex environments requires motor planning, motor preparation and the coordination between multiple sensory–motor modalities. For example, the stepping motion when we walk is coordinated with motion of the torso, arms, head and eyes. In rodents, movement of the animal through the environment is often coordinated with whisking. Here we trained head fixed mice – navigating a floating Airtrack plus maze – to overcome their directional preference and use cues indicating the direction of movement expected in each trial. Once cued, mice had to move backward out of a lane, then turn in the correct direction, and enter a new lane. In this simple paradigm, as mice begin to move backward, they position their whiskers asymmetrically: whiskers on one side of the face protract, and on the other side they retract. This asymmetry reflected the turn direction. Additionally, on each trial, mice move their eyes conjugately in the direction of the upcoming turn. Not only do they move their eyes, but saccadic eye movement is coordinated with the asymmetric positioning of the whiskers. Our analysis shows that the asymmetric positioning of the whiskers predicts the direction of turn that mice will make at an earlier stage than eye movement does. We conclude that, when mice move or plan to move in complex real-world environments, their motor plan and behavioral state can be read out in the movement of both their whiskers and eyes.Significance statementNatural behavior occurs in multiple sensory and motor dimensions. When we move through our environment we coordinate the movement of our body, head, eyes and limbs. Here we show that when mice navigate a maze, they move their whiskers and eyes; they position their whiskers asymmetrically, and use saccadic eye movements. The position of the eyes and whiskers predicts the direction mice will turn in. This work suggests that when mice move through their environment, they coordinate the visual-motor and somatosensory-motor systems.
- Published
- 2021
8. Opposite forms of adaptation in mouse visual cortex are controlled by distinct inhibitory microcircuits
- Author
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Tristan G, Heintz, Antonio J, Hinojosa, Sina E, Dominiak, and Leon, Lagnado
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Cerebral Cortex ,Mice ,Parvalbumins ,Interneurons ,Pyramidal Cells ,Animals ,Locomotion ,Visual Cortex - Abstract
Sensory processing in the cortex adapts to the history of stimulation but the mechanisms are not understood. Imaging the primary visual cortex of mice we find here that an increase in stimulus contrast is not followed by a simple decrease in gain of pyramidal cells; as many cells increase gain to improve detection of a subsequent decrease in contrast. Depressing and sensitizing forms of adaptation also occur in different types of interneurons (PV, SST and VIP) and the net effect within individual pyramidal cells reflects the balance of PV inputs, driving depression, and a subset of SST interneurons driving sensitization. Changes in internal state associated with locomotion increase gain across the population of pyramidal cells while maintaining the balance between these opposite forms of plasticity, consistent with activation of both VIP-SST and SST-PV disinhibitory pathways. These results reveal how different inhibitory microcircuits adjust the gain of pyramidal cells signalling changes in stimulus strength.
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- 2021
9. Whisking Asymmetry Signals Motor Preparation and the Behavioral State of Mice
- Author
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Mostafa A. Nashaat, Robert N. S. Sachdev, Sina E. Dominiak, Matthew E. Larkum, Keisuke Sehara, Hatem Oraby, and Colomb, Julien
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0301 basic medicine ,Visual perception ,Computer science ,media_common.quotation_subject ,Whiskers ,Somatosensory system ,Asymmetry ,Signal ,03 medical and health sciences ,0302 clinical medicine ,Whisker ,motor planning ,medicine ,Movement (clockwork) ,Central function ,Nose ,Research Articles ,media_common ,030304 developmental biology ,Facial expression ,0303 health sciences ,vibrissae ,General Neuroscience ,Whisking in animals ,Behavioral state ,decoding behavior ,attention ,030104 developmental biology ,medicine.anatomical_structure ,Licking ,Neuroscience ,030217 neurology & neurosurgery ,Tactile sensor ,psychological phenomena and processes - Abstract
A central function of the brain is to plan, predict, and imagine the effect of movement in a dynamically changing environment. Here we show that in mice head-fixed in a plus-maze, floating on air, and trained to pick lanes based on visual stimuli, the asymmetric movement, and position of whiskers on the two sides of the face signals whether the animal is moving, turning, expecting reward, or licking. We show that (1) whisking asymmetry is coordinated with behavioral state, and that behavioral state can be decoded and predicted based on asymmetry, (2) even in the absence of tactile input, whisker positioning and asymmetry nevertheless relate to behavioral state, and (3) movement of the nose correlates with asymmetry, indicating that facial expression of the mouse is itself correlated with behavioral state. These results indicate that the movement of whiskers, a behavior that is not instructed or necessary in the task, can inform an observer about what a mouse is doing in the maze. Thus, the position of these mobile tactile sensors reflects a behavioral and movement-preparation state of the mouse.SIGNIFICANCE STATEMENTBehavior is a sequence of movements, where each movement can be related to or can trigger a set of other actions. Here we show that, in mice, the movement of whiskers (tactile sensors used to extract information about texture and location of objects) is coordinated with and predicts the behavioral state of mice: that is, what mice are doing, where they are in space, and where they are in the sequence of behaviors.
- Published
- 2019
- Full Text
- View/download PDF
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