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Results demonstrate that cell shape has indirect effects on positioning of the septum by spindle extension. Data reveal that in spherical cells, the cell division scars and septa lie in an angular position with respect to each other.

The dimorphic cycle of the fission yeast Schizosaccharomyces japonicus var japonicus and transition between unipolar and bipolar growth are modulated by the nutritional gradient in the environment. Transformation from the yeast form to the mycelial morphology is correlated with widespread vacuolation. The yeast form is unstable on solid media and prone to transform to mycelium. Schizosaccharomyces japonicus var japonicus can therefore be used in the study of yeast morphogenesis and polarity.

Research was conducted to illustrate how the mutant allele sep1-1 uncouples nuclear division and cell division in Schizosaccharomyces pombe. Genetic interactions were observed between sep1-1 and the M-phase initiation mutations wee1-, cdc2-1w and cdc25-22. Dikaryon cells are formed by double mutants at high frequency. Results show that the initiation of mitosis and cell division are linked together by the genes wee1+, cdc2+, cdc25+ and sep1+.

Simple and efficient genotyping methods are widely used to assess the diversity of a large number of microbial strains, e.g. wine yeasts isolated from a specific geographical area or a vintage. Such methods are often also the first to be applied, to decrease the number of strains deemed interesting for a more time-consuming physiological characterization. Here, we aimed to use a physiologically characterized strain collection of 69 Saccharomyces cerevisiae strains from Hungarian wine regions to determine whether geographical origin or physiological similarity can be recovered by clustering the strains with one or two simultaneously used variations of interdelta genotyping. Our results indicate that although a detailed clustering with high resolution can be achieved with this method, the clustering of strains is largely contrasting when different primer sets are used and it does not recover geographical or physiological groups. Significance and Impact of the Study Genotyping is routinely used for assessing the diversity of a large number of isolates/strains of a single species, e.g. a collection of wine yeasts. We tested the efficiency of interdelta genotyping on a collection of Saccharomyces wine yeasts from four wine regions of Hungary that was previously characterized physiologically. Interdelta fingerprinting recovered neither physiological nor geographical similarities, and in addition, the two different primer pairs widely used for this method showed conflicting and barely comparable results. Thus, this method does not necessarily represent the true diversity of a strain collection, but detailed clustering may be achieved by the combined use of primer sets. [ABSTRACT FROM AUTHOR]