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A simple and novel sensor of hydrogen peroxide (H2O2) was designed successfully. Horseradish peroxidase (HRP) is effectively immobilized on the surface of chitosan (CHI)-stabilized zinc sulphide (ZnS)/graphene (G) nanocomposites modified carbon paper electrode (CPE). The interaction between HRP enzyme and H2O2 was studied from modified CPE. The catalytic performance of the biosensor was examined using cyclic voltammetry (CV) and the obtained results indicated that the prepared ZnS/G/CHI/HRP/NA/CPE nanocomposites material holds excellent catalytic performance for the H2O2 detection. The proposed biosensor showed good analytical performance and long-term storage stability. The repeatability of the sensor is checked up to eight cycles. [ABSTRACT FROM AUTHOR]

The wound dressing material has been fabricated with the novelArothron stellatusfish skin collagen scaffold loaded with a bioactive extract obtained fromCoccinia grandis(CPE) and drug Ciprofloxacin (D). The collagen scaffold was fabricated from the nonedible solid waste of marine origin (a cheaper source of raw material). Thein vitrofluorescence staining of cells andin vivoevaluation of the fabricated scaffold exhibited enhanced cell adhesion and proliferation and indicated a more efficient wound healing paradigm than that of the control and other treated groups, respectively. The increased collagen synthesis and re-epithelialization of wound are proved to be potential wound constructs. [ABSTRACT FROM AUTHOR]

Pro-regenerative patches are appealing for cornea injuries owing to their robustness, and ease of use in clinical settings. Collagen-based synthetic patches are building blocks for corneal repair, but their cross-linker toxicity, less optical clarity, and deformation instability remain unresolved. In response to these challenges, the study introduces disulfide crosslinked thiolated collagen patches (CSH). Human umbilical cord collagen (UC) comprises abundant lysine; thus, more primary amines are available for thiolation. UC was thiol functionalized, whereby the degree of amine substitution is 38 %, and a 40.4 % increase in disulfide bonds confirms disulfide-mediated crosslinking. CSH exhibits remarkable biomechanics (3.3 MPa), adhesion strength (107.25 kPa), optical clarity (87 %), and proteolytic resistance compared to native collagen. The patches support sustained levofloxacin release for three days. HET-CAM assay and hemocompatibility confirmed non-irritancy. Ex vivo adhesion, drug permeation, and epithelial integrity validated their compatibility in rabbit eyeballs. In vitro studies in human corneal stromal cells evidence that the thiols mimic endogenous peptides, favoring cell adhesion and proliferation. The patches alleviate the fibrotic episodes by reducing TGFβ1 and vimentin, which attenuates the JNK signaling, leading to elevated expression of corneal clarity restoring proteins, TGFβ3 and Keratocan. Thus, disulfide-bonded collagen patches would be an effective therapeutic modality for stromal injury-associated blindness., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2025. Published by Elsevier B.V.)

The present study demonstrates the in vivo soft tissue regenerative potential of flax seed mucilage (FSM) reinforced collagen aerogels in Wistar rats. The physiochemical, mechanical, and thermal properties were significantly improved upon the incorporation of flax mucilage into collagen when compared to the native collagen scaffold. In addition, the functional group of flax mucilage notably contributed to a better anti-oxidative potential than the control collagen. The flax mucilage-reinforced collagen at 4 mg ml -1 concentration showed a 2-fold increase in porosity compared to native collagen. The tensile strength of native collagen, 2 mg ml -1 , and 4 mg ml -1 FSM reinforced collagen was 5.22 MPa, 9.76 MPa, and 11.16 MPa, respectively, which indicated that 2 mg ml -1 and 4 mg ml -1 FSM showed an 87% and 113% percentage increase respectively in tensile strength compared to the native collagen control. FSM-reinforced biomatrix showed 97% wound closure on day 15 post-wounding, indicating faster healing than controls, where complete healing occurred only on day 21. The mechanical properties of skin treated with FSM-reinforced collagen scaffold post-healing were considerably better than native collagen. The histological and immunohistochemistry analysis also showed complete restoration of wounded tissue like intact normal skin. The findings paved the way for the development of collagen-polysaccharide mucilage wound dressing materials and their further application in skin tissue engineering., (© 2024 IOP Publishing Ltd.)