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24 results on '"Sivun, Dmitry"'

5. Purification Analysis, Intracellular Tracking, and Colocalization of Extracellular Vesicles Using Atomic Force and 3D Single-Molecule Localization Microscopy

Author

Puthukodan, Sujitha, primary, Hofmann, Martina, additional, Mairhofer, Mario, additional, Janout, Hannah, additional, Schurr, Jonas, additional, Hauser, Fabian, additional, Naderer, Christoph, additional, Preiner, Johannes, additional, Winkler, Stephan, additional, Sivun, Dmitry, additional, and Jacak, Jaroslaw, additional

Published
2023
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7. Label‐free characterization of an extracellular vesicle‐based therapeutic

Author

Priglinger, Eleni, primary, Strasser, Juergen, additional, Buchroithner, Boris, additional, Weber, Florian, additional, Wolbank, Susanne, additional, Auer, Daniela, additional, Grasmann, Eva, additional, Arzt, Claudia, additional, Sivun, Dmitry, additional, Grillari, Johannes, additional, Jacak, Jaroslaw, additional, Preiner, Johannes, additional, and Gimona, Mario, additional

Published
2021
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9. Dual Channel Microfluidics for Mimicking the Blood–Brain Barrier

Author

Buchroithner, Boris, primary, Mayr, Sandra, additional, Hauser, Fabian, additional, Priglinger, Eleni, additional, Stangl, Herbert, additional, Santa-Maria, Ana Raquel, additional, Deli, Maria A., additional, Der, Andras, additional, Klar, Thomas A., additional, Axmann, Markus, additional, Sivun, Dmitry, additional, Mairhofer, Mario, additional, and Jacak, Jaroslaw, additional

Published
2021
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10. Optical Coulomb blockade lifting in plasmonic nanoparticle dimers

Author

Sivun, Dmitry and Klar, Thomas A.

Subjects
Coulomb blockade, Physics::Optics, Condensed Matter::Mesoscopic Systems and Quantum Hall Effect, plasmonic nanoparticle
Abstract

If two metal nanoparticles are ultimately approached, a tunneling current prevents both an infinite redshift of the bonding dipolar plasmon and an infinite increase of the electric field in the hot spot between the nanoparticles. We argue that a Coulomb blockade suppresses the tunneling current and sustains a redshift even for sub-nanometer approach up to moderate fields. Only for stronger optical fields, the Coulomb blockade is lifted and a charge transfer plasmon is formed. Numerical simulations show that such scenarios are well in reach with manageable nanoparticle dimensions, even at room temperature. Applications may include ultrafast, optically driven switches, photo detectors operating at 500 THz, or highly nonlinear devices. (VLID)4926414 Version of record

Published
2020

14. Plasmonic Horizon in Gold Nanosponges

Author

Vidal, Cynthia, primary, Sivun, Dmitry, additional, Ziegler, Johannes, additional, Wang, Dong, additional, Schaaf, Peter, additional, Hrelescu, Calin, additional, and Klar, Thomas A., additional

Published
2018
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22. Synthesis, Separation, and Hypermethod Characterization of Gold Nanoparticle Dimers Connected by a Rigid Rod Linker

Author

Fruhnert, Martin, primary, Kretschmer, Florian, additional, Geiss, Reinhard, additional, Perevyazko, Igor, additional, Cialla-May, Dana, additional, Steinert, Michael, additional, Janunts, Norik, additional, Sivun, Dmitry, additional, Hoeppener, Stephanie, additional, Hager, Martin D., additional, Pertsch, Thomas, additional, Schubert, Ulrich S., additional, and Rockstuhl, Carsten, additional

Published
2015
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23. CRISPR/Cas9 Genome Editing vs. Over-Expression for Fluorescent Extracellular Vesicle-Labeling: A Quantitative Analysis.

Author

Strohmeier, Karin, Hofmann, Martina, Hauser, Fabian, Sivun, Dmitry, Puthukodan, Sujitha, Karner, Andreas, Sandner, Georg, Le Renard, Pol-Edern, Jacak, Jaroslaw, and Mairhofer, Mario

Subjects
GENOME editing, GREEN fluorescent protein, EXTRACELLULAR vesicles, FLUORESCENCE microscopy, QUANTITATIVE research, MEMBRANE lipids
Abstract

Over-expression of fluorescently-labeled markers for extracellular vesicles is frequently used to visualize vesicle up-take and transport. EVs that are labeled by over-expression show considerable heterogeneity regarding the number of fluorophores on single particles, which could potentially bias tracking and up-take studies in favor of more strongly-labeled particles. To avoid the potential artefacts that are caused by over-expression, we developed a genome editing approach for the fluorescent labeling of the extracellular vesicle marker CD63 with green fluorescent protein using the CRISPR/Cas9 technology. Using single-molecule sensitive fluorescence microscopy, we quantitatively compared the degree of labeling of secreted small extracellular vesicles from conventional over-expression and the CRISPR/Cas9 approach with true single-particle measurements. With our analysis, we can demonstrate a larger fraction of single-GFP-labeled EVs in the EVs that were isolated from CRISPR/Cas9-modified cells (83%) compared to EVs that were isolated from GFP-CD63 over-expressing cells (36%). Despite only single-GFP-labeling, CRISPR-EVs can be detected and discriminated from auto-fluorescence after their up-take into cells. To demonstrate the flexibility of the CRISPR/Cas9 genome editing method, we fluorescently labeled EVs using the HaloTag® with lipid membrane permeable dye, JaneliaFluor® 646, which allowed us to perform 3D-localization microscopy of single EVs taken up by the cultured cells. [ABSTRACT FROM AUTHOR]

Published
2022
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24. New buffer systems for photopainting of single biomolecules.

Author

Naderer C, Krobath H, Sivun D, Gvindzhiliia G, Klar TA, and Jacak J

Abstract

We present newly developed buffer systems that significantly improve the efficiency of a photochemically induced surface modification at the single molecule level. Buffers with paramagnetic cations and radical oxygen promoting species facilitate laser-assisted protein adsorption by photobleaching (LAPAP) of single fluorescently labelled oligonucleotides or biotin onto multi-photon-lithography-structured 2D and 3D acrylate scaffolds. Single molecule fluorescence microscopy has been used to quantify photopainting efficiency. We identify specific cation interaction sites for members of the cyanine, coumarin and rhodamine classes of fluorophores using quantum mechanical calculations. We show that our buffer systems provide an up to three-fold LAPAP-efficiency increase for the cyanine fluorophore, while keeping excitation parameters constant., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Published
2023
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