22 results on '"Siyu Pu"'
Search Results
2. Dry Powder Inhaler Formulation of Lactobacillus rhamnosus GG Targeting Pseudomonas aeruginosa Infection in Bronchiectasis Maintenance Therapy
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The-Thien Tran, Wean Sin Cheow, Siyu Pu, Jin-Won Park, and Kunn Hadinoto
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dry powder inhaler ,pulmonary drug delivery ,lung infection ,probiotics ,Pharmacy and materia medica ,RS1-441 - Abstract
The inhaled delivery of lactic acid bacteria (LAB) probiotics has been demonstrated to exert therapeutic benefits to the lungs due to LAB’s immunomodulatory activities. The development of inhaled probiotics formulation, however, is in its nascent stage limited to nebulized LAB. We developed a dry powder inhaler (DPI) formulation of lactobacillus rhamnosus GG (LGG) intended for bronchiectasis maintenance therapy by spray freeze drying (SFD). The optimal DPI formulation (i.e., LGG: mannitol: lactose: leucine = 35: 45: 15: 5 wt.%) was determined based on the aerosolization efficiency (86% emitted dose and 26% respirable fraction) and LGG cell viability post-SFD (7 log CFU/mL per mg powder). The optimal DPI formulation was evaluated and compared to lyophilized naked LGG by its (1) adhesion capacity and cytotoxicity to human lung epithelium cells (i.e., A549 and 16HBE14o- cells) as well as its (2) effectiveness in inhibiting the growth and adhesion of Pseudomonas aeruginosa to lung cells. The optimal DPI of LGG exhibited similar non-cytotoxicity and adhesion capacity to lung cells to naked LGG. The DPI of LGG also inhibited the growth and adhesion of P. aeruginosa to the lung cells as effectively as the naked LGG. The present work established the feasibility of delivering the LAB probiotic by the DPI platform without adversely affecting LGG’s anti-pseudomonal activities.
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- 2024
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3. A novel smoothed (SMO) point mutation in congenital tibial hemimelia: a case report
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Xiaodong Yang, Siyu Pu, Bo Xiang, Xueyang Tang, and Jing Chen
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Tibial hemimelia ,Congenital anomaly ,SMO ,Polydactyly ,Pediatrics ,RJ1-570 - Abstract
Abstract Background Congenital tibial hemimelia (CTH [MIM: 275220]) is a rare congenital limb deficiency that manifests as a shortened, curved, dysplastic or absent tibia with polydactyly. In previous studies, mutations of a distant sonic hedgehog (SHH) cis-regulator (ZRS) and a Shh repressor (GLI3) were identified. Case presentation Here, we admitted a 20-month-old boy who manifested with right tibial deformity, varus foot, ankle dislocation, and ipsilateral preaxial polydactyly. After genetic sequencing and data analysis, the results revealed a 443 A > G mutation in the father and a 536 C > T mutation in the mother in exon 2 of the Smoothed (SMO) gene at 7q32.1, with the coexistence of both mutant alleles in the proband/patient. Conclusions Our report suggests that even though not previously reported, SMO mutations may be associated with limb anomalies such as tibial hemimelia via Hh signaling in humans and has implications for genetic counseling.
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- 2023
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4. Effects of Alkaline Extraction pH on Amino Acid Compositions, Protein Secondary Structures, Thermal Stability, and Functionalities of Brewer’s Spent Grain Proteins
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Kunn Hadinoto, Jordy Kim-Ung Ling, Siyu Pu, and The-Thien Tran
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biological macromolecules ,alternative proteins ,protein extraction ,alkaline extraction ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
A high alkaline pH was previously demonstrated to enhance the extraction yield of brewer’s spent grains (BSG) proteins. The effects of extraction pH beyond the extraction yield, however, has not been investigated before. The present work examined the effects of extraction pH (pH 8–12) on BSG proteins’ (1) amino acid compositions, (2) secondary structures, (3) thermal stability, and (4) functionalities (i.e., water/oil holding capacity, emulsifying, and foaming properties). The ideal extraction temperature (60 °C) and BSG-to-solvent ratio (1:20 w/v) for maximizing the extraction yield were first determined to set the conditions for the pH effect study. The results showed that a higher extraction pH led to more balanced compositions between hydrophilic and hydrophobic amino acids and higher proportions of random coils structures indicating increased protein unfolding. This led to superior emulsifying properties of the extracted proteins with more than twofold improvement between pH 8 and a pH larger than 10. The extraction pH, nevertheless, had minimal impact on the water/oil holding capacity, foaming properties, and thermal denaturation propensity of the proteins. The present work demonstrated that a high alkaline pH at pH 11–12 was indeed ideal for both maximizing the extraction yield (37–46 wt.%) and proteins’ functionalities.
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- 2024
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5. Corrigendum: Molecular characteristics of choledochal cysts in children: transcriptome sequencing
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Yong Lv, Xiaolong Xie, Lihui Pu, Qi Wang, Jiayin Yang, Siyu Pu, Chengbo Ai, Yi Liu, Jing Chen, and Bo Xiang
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transcriptome ,choledochal cysts ,weighted gene co-expression network analysis ,children ,bioinformatics ,Genetics ,QH426-470 - Published
- 2023
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6. Case Report: Pancreatic and hepatic kaposiform hemangioendothelioma presenting as consumptive coagulopathy and right hepatic atrophy
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Chengbo Ai, Tong Qiu, Jiangyuan Zhou, Chuan Wang, Jiulin Song, Siyu Pu, and Shuguang Jin
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Kaposiform hemangioendothelioma (KHE) ,Kasabach-Merritt phenomenon (KMP) ,vascular tumor ,Whipple operation ,thrombocytopenia ,case report ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Kaposiform hemangioendothelioma (KHE) is a rare vascular tumor that causes progressive angiogenesis and lymphangiogenesis, which often occurs in the skin or soft tissue, with an acute onset and rapid progression. A 4-year-old girl was admitted to our hospital with a 2-year history of thrombocytopenia, combined with right hepatic atrophy and pancreatic lesion for 3 months. At the age of two, she developed purpura and thrombocytopenia was detected, after treatment with gamma globulin and corticosteroids, the platelet count normalized, but it dropped immediately at lower doses. One year after the cessation of corticosteroids therapy, the patient presented with abdominal pain and abnormal liver function and the magnetic resonance imaging (MRI) revealed right hepatic atrophy and pancreatic occupancy, but the first liver biopsy did not reveal any positive pathological results. By analyzing the clinical manifestations in conjunction with MRI and abnormal coagulation, we considered that the patient might be diagnosed as KHE with Kasabach-Merritt phenomenon, however, sirolimus treatment was ineffective and pancreatic biopsy only showed a tendency for tumors of vascular origin. Finally, we performed a Whipple operation after the right hepatic artery embolization, histological and immunohistochemical examination suggested KHE. Three months postoperatively, the patient’s liver function, pancreatic enzymes and blood clotting function gradually returned to normal. KHEs may result in significant blood loss with worsening of the coagulopathy and functional impairment, timely surgical intervention for KHE is necessary when non-invasive or minimally invasive treatment is ineffective, or the symptoms of tumor compression are obvious.
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- 2023
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7. A Novel Model for Predicting the Clearance of Jaundice in Patients With Biliary Atresia After Kasai Procedure
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Yimao Zhang, Qi Wang, Siyu Pu, Junxiang Wang, Bo Xiang, Juxian Liu, and Shuguang Jin
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biliary atresia ,Kasai ,clearance of jaundice ,nomogram ,prediction model ,Pediatrics ,RJ1-570 - Abstract
BackgroundThe failed clearance of jaundice (CJ) in patients with biliary atresia (BA) after the Kasai procedure (KP) often leads to a shorter native liver survival (NLS) time and earlier liver transplantation. We aimed to investigate risk factors of failed CJ and establish a novel nomogram model to predict the status of CJ.MethodsWe retrospectively reviewed institutional medical records from January 2015 to April 2020 and enrolled BA patients post-KP, randomly divided into training and testing cohorts at a ratio of 7:3, and further subdivided into cleared and uncleared jaundice groups. Univariate and multiple logistic regression analyses were used to select risk factors to establish the nomogram in the training cohort. The performance of the nomogram was evaluated by calculating the areas under the receiver operating curve (AUC) in both cohorts.ResultsThis study included 175 BA patients post-KP. After univariate and multiple logistic regression analyses, Cytomegalovirus IgM +ve associated BA (OR = 3.38; 95% CI 1.01–11.32; P = 0.04), ln γ-glutamyl transpeptidase (GGT) (OR = 0.41; 95% CI 0.22–0.80; P = 0.009), thickness of the fibrous portal plate (OR = 0.45; 95% CI 0.27–0.76; P = 0.003), liver stiffness measurement (LSM) (OR = 1.19; 95% CI 1.06–1.34; P = 0.002), and multiple episodes of cholangitis (OR = 1.65; 95% CI 1.13–2.41; P = 0.01) were identified as independent risk factors of unsuccessful CJ to construct the nomogram. The receiver operating characteristic curve (ROC) analysis suggested good nomogram performance in both the training (AUC = 0.96) and testing cohorts (AUC = 0.91).ConclusionOur nomogram model including several risk factors effectively predicts CJ in patients post-KP, which could aid in clinical decision-making.
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- 2022
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8. Lyophilization of Curcumin–Albumin Nanoplex with Sucrose as Cryoprotectant: Aqueous Reconstitution, Dissolution, Kinetic Solubility, and Physicochemical Stability
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Angeline Chua, The-Thien Tran, Siyu Pu, Jin-Won Park, and Kunn Hadinoto
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curcumin nanoparticles ,albumin nanoparticles ,amorphous drugs ,freeze drying ,protein stability ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
An amorphous curcumin (CUR) and bovine serum albumin (BSA) nanoparticle complex (nanoplex) was previously developed as a promising anticancer nanotherapy. The CUR-BSA nanoplex had been characterized in its aqueous suspension form. The present work developed a dry-powder form of the CUR-BSA nanoplex by lyophilization using sucrose as a cryoprotectant. The cryoprotective activity of sucrose was examined at sucrose mass fractions of 33.33, 50.00, and 66.66% by evaluating the lyophilized nanoplex’s (1) aqueous reconstitution and (2) CUR dissolution and kinetic solubility. The physicochemical stabilizing effects of sucrose upon the nanoplex’s 30-day exposures to 40 °C and 75% relative humidity were examined from (i) aqueous reconstitution, (ii) CUR dissolution, (iii) CUR and BSA payloads, (iv) amorphous form stability, and (v) BSA’s structural integrity. The good cryoprotective activity of sucrose was evidenced by the preserved BSA’s integrity and good aqueous reconstitution, resulting in a fast CUR dissolution rate and a high kinetic solubility (≈5–9× thermodynamic solubility), similar to the nanoplex suspension. While the aqueous reconstitution, CUR dissolution, and amorphous form were minimally affected by the elevated heat and humidity exposures, the treated nanoplex exhibited a lower BSA payload (≈7–26% loss) and increased protein aggregation postexposure. The adverse effects on the BSA payload and aggregation were minimized at higher sucrose mass fractions.
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- 2022
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9. Molecular Characteristics of Choledochal Cysts in Children: Transcriptome Sequencing
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Yong Lv, Xiaolong Xie, Lihui Pu, Qi Wang, Jiayin Yang, Siyu Pu, Chengbo Ai, Yi Liu, Jing Chen, and Bo Xiang
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transcriptome ,choledochal cysts ,weighted gene co-expression network analysis ,children ,bioinformatics ,Genetics ,QH426-470 - Abstract
A choledochal cyst (CC) is a common congenital biliary disease in children, yet the underlying molecular bases for the cystic and fusiform clinical subtypes are unknown. RNA sequencing (RNA-seq) has been performed on 22 high-quality CC samples, including 12 cystic CC and 10 fusiform CC samples, to search for molecular features. Weighted gene co-expression network analysis (WGCNA) was performed to identify key modules associated with clinical subtypes. Bioinformatic analyses were conducted to elucidate potential mechanisms. Then, we constructed protein–protein interaction (PPI) networks to identify candidate hub genes related to CC. Finally, we used the support vector machine (SVM) to eliminate redundant features and screen out the hub genes. The selected gene expression was determined in CC patients through quantitative real-time polymerase chain reaction (PCR). A total of 6,463 genes were found to be aberrantly expressed between cystic CC and fusiform CC. Twelve co-expression modules that correlated with clinical subtypes of CC were identified and assigned representative colors. Among the 12 modules, the blue module was considered the key module. Two functionally distinct sets of dysregulated genes have been identified in two major subtypes, metabolism-related genes in cystic CC and immune-related genes in fusiform CC. A total of 20 candidate hub genes that were correlated with clinical subtypes were found in the blue module. In addition, we found ERBB2 and WNT11 that have not been studied in CC and verified their differential expression in CC through quantitative real-time PCR experiments. For the first time, we have described the transcriptome characteristics of CC. These results suggest that cystic CC and fusiform CC have different molecular mechanisms. The bi-omics-identified novel candidate genes and pathways might be helpful for personalized treatment and are of great clinical significance for CC.
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- 2021
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10. A novel structural variant of RET causes familial Hirschsprung's disease via nonsense-mediated mRNA decay
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Qi Wang, Siyu Pu, Bo Xiang, and Jing Chen
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Cell Biology ,Molecular Biology ,Biochemistry ,Genetics (clinical) - Published
- 2023
11. A comparative study of antisolvent versus salting-out precipitations of glycopeptide vancomycin: precipitation efficiency and product qualities
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Siyu Pu, Kunn Hadinoto, and School of Chemistry, Chemical Engineering and Biotechnology
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General Chemical Engineering ,Chemical engineering [Engineering] ,Protein Precipitation ,Peptide Purification - Abstract
Precipitation has been studied as an alternative downstream processing step of biopharmaceuticals to replace chromatography. In protein precipitation, previous studies showed precipitation efficiency and product qualities were influenced by the precipitation agents (e.g., salts, organic solvents, polymers). For bioactive peptides, however, the impacts of precipitation agents had rarely been studied. We previously studied salting-out precipitation of glycopeptide vancomycin (Van) used as the model peptide. The present work compared antisolvent (with acetone) and salting-out precipitations in their precipitation efficiency and product qualities. The phase behavior study showed that heavy precipitates composed of nanoparticles were the predominant products of antisolvent precipitation, in contrast to crystalline microparticles produced by salting-out. At their respective optimal conditions (e.g., pH, Van concentration), batch antisolvent precipitation had significantly higher yield than salting-out. Other than faster dissolution of antisolvent precipitates attributed to their smaller size, both precipitates exhibited comparable purity, peptide secondary structures, thermal stability, and antimicrobial activity. Ministry of Education (MOE) The authors would like to acknowledge the research funding from Ministry of Education Singapore (Grant No. AcRF RG82/20).
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- 2023
12. Comparative evaluations of bulk seeded protein crystallization in batch versus continuous slug flow crystallizers
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Siyu Pu, Kunn Hadinoto, and School of Chemical and Biomedical Engineering
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Supersaturation ,Continuous Crystallization ,Protein Crystallization ,Materials science ,General Chemical Engineering ,Chemical engineering [Engineering] ,Nucleation ,Crystal growth ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Slug flow ,01 natural sciences ,0104 chemical sciences ,law.invention ,Volumetric flow rate ,Crystal ,Chemical engineering ,law ,Crystallization ,0210 nano-technology ,Protein crystallization - Abstract
The present work evaluated for the first time the use of continuous slug flow crystallizer (CSFC) in bulk seeded protein crystallization, using lysozyme (LYZ) as the model protein. LYZ crystals pre-prepared in batch crystallizer were used as seeds. The crystallization was performed at supersaturation level below the metastable-zone-limit. The resultant crystal size distribution (CSD), CSD's reproducibility, LYZ's bioactivity, and crystallization efficiency as characterized by the space-time-yield (STY, mg/h·L) were determined. The results showed that the CSFC's performance was governed by the flowrate, where a trade-off existed between crystal quality and crystallization efficiency upon varying the flowrate. At low flowrates, which reduced the shear rate and prolonged the residence time, well-defined large tetragonal crystals were produced attributed to suppressed secondary nucleation and extended crystal growth, but low STY due to difficulty in transporting the seeds/products crystals. Higher flow rates led to higher STY, but increased production of small non-tetragonal crystals that formed agglomerates. Compared to batch crystallizer, the CSFC produced LYZ crystals of similar average size, morphology, and bioactivity, but with roughly 50% lower STY, as the batch's high-shear environment promoted secondary nucleation, hence higher crystallization rate. The CSD's width and reproducibility was nevertheless significantly improved in CSFC. Ministry of Education (MOE) The authors would like to acknowledge the funding from Ministry of Education Singapore under Academic Research Fund Tier 1RG82/20 (2021).
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- 2021
13. Maintaining supersaturation generation and protein integrity of amorphous curcumin-albumin nanoplex during storage by freeze drying with trehalose
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The-Thien Tran, Angeline Chua, Siyu Pu, Jin-Won Park, and Kunn Hadinoto
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Pharmaceutical Science - Published
- 2023
14. The Role of GLI in the Regulation of Hepatic Epithelial–Mesenchymal Transition in Biliary Atresia
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Siyu, Pu, primary, Junxiang, Wang, additional, Qi, Wang, additional, Yimao, Zhang, additional, and Shuguang, Jin, additional
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- 2022
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15. Continuous crystallization as a downstream processing step of pharmaceutical proteins: A review
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Siyu Pu, Kunn Hadinoto, and School of Chemical and Biomedical Engineering
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Bioengineering [Engineering] ,Downstream processing ,Materials science ,business.industry ,General Chemical Engineering ,02 engineering and technology ,General Chemistry ,Biologics ,Raw material ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Slug flow ,01 natural sciences ,Bottleneck ,Biopharmaceuticals ,0104 chemical sciences ,law.invention ,law ,Continuous crystallization ,Yield (chemistry) ,Crystallization ,0210 nano-technology ,Process engineering ,business ,Protein crystallization - Abstract
Continuous crystallization has been proposed as the downstream processing step for the purification of pharmaceutical proteins aimed at alleviating the manufacturing bottleneck caused by the limitations of chromatography-based operations at high upstream production titers. Herein we reviewed the current state of research in continuous protein crystallization from which future research directions were identified. While the benefits of batch-to-continuous manufacturing transformation have been long established, progress in continuous protein crystallization lags behind its small-molecule counterpart. The reasons are because the challenging nature of protein crystallization, even when performed in the batch platform, and the lack of well-understood proteins available for thorough study. Nevertheless, successful batch-to-continuous transformations in both mixed-suspension-mixed-product-removal crystallizer and tubular crystallizers (i.e. slug flow, oscillatory baffled flow) have been demonstrated using lysozyme or monoclonal antibody as the model protein. Compared to the batch platform, the continuous platform produces comparable crystallization yield but with higher production capacity (g/h). Strategies to optimize the crystallizer's performance based on modeling and simulation results are also available. Future research should (1) study a wider range of proteins with impurities incorporated in the raw material streams, and (2) adopt advancements in continuous crystallization of small-molecule pharmaceuticals to improve the crystal quality and yield. Ministry of Education (MOE) The authors would like to thank the Ministry of Education Singapore for funding this work (Grant No. RG7/17 PI: Kunn Hadinoto Ong).
- Published
- 2020
16. A calcium-based microporous metal-organic framework for efficient adsorption separation of light hydrocarbons
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Qiwei Yang, Zongbi Bao, Zhiguo Zhang, Siyu Pu, Qilong Ren, Jiawei Wang, Yiwen Yang, Lidong Guo, Sufian Alnemrat, and Liangying Li
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chemistry.chemical_classification ,Materials science ,General Chemical Engineering ,02 engineering and technology ,General Chemistry ,Squaric acid ,Microporous material ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Industrial and Manufacturing Engineering ,0104 chemical sciences ,chemistry.chemical_compound ,Adsorption ,Hydrocarbon ,chemistry ,Chemical engineering ,Environmental Chemistry ,Metal-organic framework ,Thermal stability ,0210 nano-technology ,Ambient pressure ,BET theory - Abstract
Ideal metal-organic frameworks (MOFs) are those have not only significant adsorption separation performances but also excellent vapor and/or thermal stability. In this work, a calcium-based metal-organic framework with high vapor-stability and thermostability, Ca(squarate), was prepared using readily available precursor (CaCO3) and squaric acid as starting materials by hydrothermal method. Characterization techniques including PXRD, TGA and BET surface area analysis reveal that this MOF remains stable no matter whether it was soaked in water or exposed to air for as long as 2 weeks. Moreover, the studied MOF material is capable of discriminating C2 hydrocarbon molecules as it has an appropriate pore diameter around 3.4 A. Excellent efficient separation for C2H2/C2H4 mixture (50:50, v/v) with an adsorption selectivity of 8.1 was realized at room temperature and ambient pressure, on the other hand, it also displayed a good separation performance for C2H4/C4H6 mixture (50:50, v/v) with an adsorption selectivity of 5.9. Furthermore, adsorption mechanism of C2 hydrocarbons in this framework was investigated by the DFT-D calculation. The results revealed that this framework discriminated light hydrocarbons as a result of different interaction strength between hydrocarbons and framework that mainly arise from π-π interactions.
- Published
- 2019
17. The chinese tea industry under globalization : an analysis of the current situation and challenges
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Siyu, Pu, Mah, Luís, and Bernardo, Luís Pais
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China ,Mercado internacional ,International market ,Indústria do chá ,Tea industry ,Análise ,Analysis - Abstract
Mestrado em Desenvolvimento e Cooperação Internacional A China é a origem do chá, e a indústria do chá é uma indústria tradicional com uma longa história na China. A indústria do chá tem importância certa na importação e exportação da China. No entanto, a competitividade e a posição da indústria do chá chinesa no mercado internacional continuam a diminuir devido a fatores subjetivos e objetivos. Quais são as razões para o declínio da indústria do chá da primeira no mundo? Quais são a situação atual e os desafios? Esta dissertação tem como objetivo apresentar a situação atual e as características da indústria do chá chinesa, descobrir os problemas enfrentados pela indústria do chá chinesa e as razões para o declínio da competitividade sob a globalização e estender da utilização abrangente da indústria do chá ao cadeia global de valor por meio de pesquisa bibliográfica e análise qualitativa. Apresenta algumas sugestões para o desenvolvimento futuro da indústria do chá chinesa e o aumento da sua competitividade internacional, o que ajudará a indústria do chá chinesa a se internacionalizar e se juntar à cadeia global de valor. China is the hometown of tea, and tea industry is a traditional industry with a long history of China. It has a certain importance in China's import and export. However, the competitiveness and position of Chinese tea industry in the international market is continuing to decline due to subjective and objective factors.What are the reasons for the declining status of tea industry from the first in the world? What are the current situation and challenges? This paper aims to introduce the current situation and characteristics of Chinese tea industry, find out the problems faced by Chinese tea industry and the reasons for the decline in competitiveness under the background of globalization and extend from the comprehensive utilization of the tea industry to the global value chain by means of literature research and qualitative analysis. It puts forward some suggestions for the future development of Chinese tea industry and the enhancement of its international competitiveness, which will help Chinese tea industry to go international and join the global value chain. info:eu-repo/semantics/publishedVersion
- Published
- 2020
18. The current status and outcomes of pediatric liver transplantation in western China: A multi‐center retrospective study
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Wei Lai, Bin Huang, Bo Xiang, Ming-Man Zhang, Jia-Yin Yang, Chunqiang Dong, Lu-Nan Yan, Xiaolong Xie, Kewei Li, Siyu Pu, and Shuguang Jin
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Male ,China ,medicine.medical_specialty ,Adolescent ,medicine.medical_treatment ,030232 urology & nephrology ,Kaplan-Meier Estimate ,030230 surgery ,Liver transplantation ,Body weight ,03 medical and health sciences ,Liver disease ,0302 clinical medicine ,Biliary atresia ,Internal medicine ,medicine ,Humans ,Metabolic disease ,Child ,Survival analysis ,Retrospective Studies ,Transplantation ,business.industry ,Graft Survival ,Infant ,Patient survival ,Retrospective cohort study ,medicine.disease ,Liver Transplantation ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Female ,business - Abstract
This retrospective study was conducted to examine the development and current status of pediatric liver transplantation (LT) in western China. Clinical, demographic, morbidity, and mortality data were collected to analyze. It included 260 consecutive pediatric LTs performed at three centers in western China between January 2000 and May 2019. Kaplan-Meier graft survival rates at 1, 3, 5, and 10 years were 82.1%, 77.2%, 76.6%, and 76.6%, respectively; corresponding patient survival rates were 84.7%, 80.7%, 80.0%, and 80.0%, respectively. More patients underwent living donor liver transplantation (LDLT; n = 188 (73.4%)) than deceased-donor liver transplantation (DDLT; n = 68 (26.6%)). Survival was better after LDLT (91.5%, 86.6%, and 80.6% at 1, 3, and 5 years, respectively) than after DDLT (80.9%, 72.4%, and 63.9%, respectively; P < .05). Biliary atresia was the leading LT indication (n = 141 (55.1%)), followed by metabolic disease (n = 36 (14.1%)), which was associated with the best recipient survival (88.5% at 5 years). The transplant era and graft-to-recipient body weight ratio (GRWR) also significantly predicted overall survival. Survival rates at 5 years were worst in 2000-2005 (54.5%) and best for GRWRs of 0.8%-4% (80.4%). The development of pediatric LT in western China began slowly, but the quantity and quality of pediatric LT has progressed in recent years. This procedure is now a promising and reliable treatment for children with end-stage liver disease in western China.
- Published
- 2020
19. Performance Comparison of Metal–Organic Framework Extrudates and Commercial Zeolite for Ethylene/Ethane Separation
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Huabin Xing, Yiwen Yang, Zongbi Bao, Jiawei Wang, Liangying Li, Qilong Ren, Qiwei Yang, Zhiguo Zhang, and Siyu Pu
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Pressure drop ,Thermogravimetric analysis ,Ethylene ,Materials science ,Scanning electron microscope ,General Chemical Engineering ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Industrial and Manufacturing Engineering ,0104 chemical sciences ,chemistry.chemical_compound ,Adsorption ,chemistry ,Chemical engineering ,Specific surface area ,Metal-organic framework ,0210 nano-technology ,Zeolite - Abstract
Mg-MOF-74 is one of the most well-known metal–organic frameworks (MOFs) and has been widely studied for gas adsorption and separation. Nevertheless, employment of original Mg-MOF-74 in powder form inevitably leads to a high pressure drop across column and even dust pollution in practical application. In this work, we molded powder Mg-MOF-74 into extrudates and tested its performance on adsorptive separation of ethylene/ethane mixture for the first time, taking commercialized benchmark 13X zeolite as comparison. Powder X-ray diffraction (XRD), scanning electron microscopy (SEM), specific surface area measurements, and thermal gravimetric analysis (TGA) were performed to characterize the transformed samples, and adsorption equilibrium of ethylene and ethane on molded Mg-MOF-74 were measured at 273 and 298 K and pressure up to 1 bar. The potential industrial application of molded Mg-MOF-74 was examined through breakthrough and regeneration experiments, revealing that molded Mg-MOF-74 was feasible and stable ...
- Published
- 2018
20. Novel polythiophene derivative for dual-channel cell imaging
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Ni Yan, Siyu Pu, Yefei Tian, Luke Yan, Jiale Song, Junji Wei, Huiyun Xia, and Fengyan Wang
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Aqueous solution ,Materials science ,General Chemical Engineering ,Cell ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,01 natural sciences ,Porphyrin ,0104 chemical sciences ,chemistry.chemical_compound ,medicine.anatomical_structure ,Polymerization ,chemistry ,Excited state ,medicine ,Side chain ,0210 nano-technology ,Absorption (electromagnetic radiation) ,Cytotoxicity - Abstract
Fluorescent materials play an extremely important role in understanding the microbiological world. New fluorescent materials which have good photophysical properties, low cytotoxicity, and multi-channel fluorescent imaging capability are still urgently needed, even though many kinds of fluorescent materials have already been synthesized. In this work, a new polythiophene derivative (PT-OH-PPR) modified with a porphyrin group in its side chain was designed and fabricated through FeCl3 oxidative polymerization. The obtained PT-OH-PPR has wide absorption and emission spectral range, good water solubility and low cytotoxicity. Importantly it could be enriched in the cytoplasm of A549 cells and be excited by two excitation wavelengths (488 nm and 559 nm), which provides a promising application in dual-channel cell imaging.
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- 2019
21. Improving the reproducibility of size distribution of protein crystals produced in continuous slug flow crystallizer operated at short residence time
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Kunn Hadinoto, Siyu Pu, and School of Chemical and Biomedical Engineering
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Supersaturation ,Protein Crystallization ,Continuous Crystallization ,Materials science ,Yield (engineering) ,Economies of agglomeration ,Applied Mathematics ,General Chemical Engineering ,Nucleation ,02 engineering and technology ,General Chemistry ,021001 nanoscience & nanotechnology ,Residence time distribution ,Residence time (fluid dynamics) ,Slug flow ,Industrial and Manufacturing Engineering ,020401 chemical engineering ,Chemical engineering ,Chemical technology [Engineering] ,0204 chemical engineering ,0210 nano-technology ,Protein crystallization - Abstract
Continuous crystallization of proteins at short residence time and high supersaturation level is attractive in terms of the space-time yield and production efficiency. Nevertheless, it is rarely pursued due to its less-than-desirable crystal size distribution (CSD) characterized by the abundance of small crystals due to high nucleation rate. The small crystals were prone to random agglomeration, resulting in poor crystals’ residence time distribution, hence low CSD's reproducibility. Herein we developed a segmented slug flow crystallizer (SFC) design operated at short residence time (
- Published
- 2021
22. Performance Comparison of Metal-Organic Framework Extrudates and Commercial Zeolite for Ethylene/Ethane Separation.
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Siyu Pu, Jiawei Wang, Liangying Li, Zhiguo Zhang, Zongbi Bao, Qiwei Yang, Yiwen Yang, Huabin Xing, and Qilong Ren
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METAL-organic frameworks , *ZEOLITES , *METAL extrusion , *ETHYLENE , *ETHANES , *SEPARATION (Technology) - Abstract
Mg-MOF-74 is one of the most well-known metal-organic frameworks (MOFs) and has been widely studied for gas adsorption and separation. Nevertheless, employment of original Mg-MOF-74 in powder form inevitably leads to a high pressure drop across column and even dust pollution in practical application. In this work, we molded powder Mg-MOF-74 into extrudates and tested its performance on adsorptive separation of ethylene/ethane mixture for the first time, taking commercialized benchmark 13X zeolite as comparison. Powder X-ray diffraction (XRD), scanning electron microscopy (SEM), specific surface area measurements, and thermal gravimetric analysis (TGA) were performed to characterize the transformed samples, and adsorption equilibrium of ethylene and ethane on molded Mg-MOF-74 were measured at 273 and 298 K and pressure up to 1 bar. The potential industrial application of molded Mg-MOF-74 was examined through breakthrough and regeneration experiments, revealing that molded Mg-MOF-74 was feasible and stable enough to be exploited as adsorbent of adsorptive separation of ethylene/ethane. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
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