Search

Your search keyword '"Smeets, Elien"' showing total 26 results
Start Over Author "Smeets, Elien"
26 results on '"Smeets, Elien"'

2. Defective Sec61α1 underlies a novel cause of autosomal dominant severe congenital neutropenia

Author

Van Nieuwenhove, Erika, Barber, John S., Neumann, Julika, Smeets, Elien, Willemsen, Mathijs, Pasciuto, Emanuela, Prezzemolo, Teresa, Lagou, Vasiliki, Seldeslachts, Laura, Malengier-Devlies, Bert, Metzemaekers, Mieke, Haßdenteufel, Sarah, Kerstens, Axelle, van der Kant, Rob, Rousseau, Frederic, Schymkowitz, Joost, Di Marino, Daniele, Lang, Sven, Zimmermann, Richard, Schlenner, Susan, Munck, Sebastian, Proost, Paul, Matthys, Patrick, Devalck, Christine, Boeckx, Nancy, Claessens, Frank, Wouters, Carine, Humblet-Baron, Stephanie, Meyts, Isabelle, and Liston, Adrian

Published
2020
Full Text
View/download PDF

3. Validation of the Decipher Test for Predicting Distant Metastatic Recurrence in Men with High-risk Nonmetastatic Prostate Cancer 10 Years After Surgery

Author

Van den Broeck, Thomas, Moris, Lisa, Gevaert, Thomas, Tosco, Lorenzo, Smeets, Elien, Fishbane, Nick, Liu, Yang, Helsen, Christine, Margrave, Jennifer, Buerki, Christine, Davicioni, Elai, Van Poppel, Hendrik, Everaerts, Wouter, Weinmann, Sheila, Den, Robert, Davis, John, Schaeffer, Edward, Karnes, R. Jeffrey, Claessens, Frank, and Joniau, Steven

Published
2019
Full Text
View/download PDF

4. The androgen receptor depends on ligand-binding domain dimerization for transcriptional activation

Author

El Kharraz, Sarah, Dubois, Vanessa, van Royen, Martin E, Houtsmuller, Adriaan B, Pavlova, Ekatarina, Atanassova, Nina, Nguyen, Tien, Voet, Arnout, Eerlings, Roy, Handle, Florian, Prekovic, Stefan, Smeets, Elien, Moris, Lisa, Devlies, Wout, Ohlsson, Claes, Poutanen, Matti, Verstrepen, Kevin J, Carmeliet, Geert, Launonen, Kaisa-Mari, Helminen, Laura, Palvimo, Jorma J, Libert, Claude, Vanderschueren, Dirk, Helsen, Christine, and Claessens, Frank

Published
2021
Full Text
View/download PDF

5. Rational evolution for altering the ligand preference of estrogen receptor alpha.

Author

Eerlings, Roy, Gupta, Purvi, Lee, Xiao Yin, Nguyen, Tien, El Kharraz, Sarah, Handle, Florian, Smeets, Elien, Moris, Lisa, Devlies, Wout, Vandewinkel, Bram, Thiry, Irina, Ta, Duy Tien, Gorkovskiy, Anton, Voordeckers, Karin, Henckaerts, Els, Pinheiro, Vitor B., Claessens, Frank, Verstrepen, Kevin J., Voet, Arnout, and Helsen, Christine

Abstract

Estrogen receptor α is commonly used in synthetic biology to control the activity of genome editing tools. The activating ligands, estrogens, however, interfere with various cellular processes, thereby limiting the applicability of this receptor. Altering its ligand preference to chemicals of choice solves this hurdle but requires adaptation of unspecified ligand‐interacting residues. Here, we provide a solution by combining rational protein design with multi‐site‐directed mutagenesis and directed evolution of stably integrated variants in Saccharomyces cerevisiae. This method yielded an estrogen receptor variant, named TERRA, that lost its estrogen responsiveness and became activated by tamoxifen, an anti‐estrogenic drug used for breast cancer treatment. This tamoxifen preference of TERRA was maintained in mammalian cells and mice, even when fused to Cre recombinase, expanding the mammalian synthetic biology toolbox. Not only is our platform transferable to engineer ligand preference of any steroid receptor, it can also profile drug‐resistance landscapes for steroid receptor‐targeted therapies. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

8. Data from Antizyme Inhibitor 1 Regulates Matrikine Expression and Enhances the Metastatic Potential of Aggressive Primary Prostate Cancer

Author

Van den Broeck, Thomas, primary, Moris, Lisa, primary, Gevaert, Thomas, primary, Davicioni, Elai, primary, Boeckx, Bram, primary, Lambrechts, Diether, primary, Helsen, Christine, primary, Handle, Florian, primary, Ghesquière, Bart, primary, Soenen, Stefaan, primary, Smeets, Elien, primary, Eerlings, Roy, primary, El Kharraz, Sarah, primary, Devlies, Wout, primary, Karnes, R. Jeffrey, primary, Lotan, Tamara, primary, Van Poppel, Hendrik, primary, Joniau, Steven, primary, and Claessens, Frank, primary

Published
2023
Full Text
View/download PDF

9. Supplementary Tables S1-S2 and Figures S1-S15 from The Effect of F877L and T878A Mutations on Androgen Receptor Response to Enzalutamide

Author

Prekovic, Stefan, primary, van Royen, Martin E., primary, Voet, Arnout R.D., primary, Geverts, Bart, primary, Houtman, Rene, primary, Melchers, Diana, primary, Zhang, Kam Y.J., primary, Van den Broeck, Thomas, primary, Smeets, Elien, primary, Spans, Lien, primary, Houtsmuller, Adriaan B., primary, Joniau, Steven, primary, Claessens, Frank, primary, and Helsen, Christine, primary

Published
2023
Full Text
View/download PDF

10. Data from The Effect of F877L and T878A Mutations on Androgen Receptor Response to Enzalutamide

Author

Prekovic, Stefan, primary, van Royen, Martin E., primary, Voet, Arnout R.D., primary, Geverts, Bart, primary, Houtman, Rene, primary, Melchers, Diana, primary, Zhang, Kam Y.J., primary, Van den Broeck, Thomas, primary, Smeets, Elien, primary, Spans, Lien, primary, Houtsmuller, Adriaan B., primary, Joniau, Steven, primary, Claessens, Frank, primary, and Helsen, Christine, primary

Published
2023
Full Text
View/download PDF

11. Supplementary Data from Antizyme Inhibitor 1 Regulates Matrikine Expression and Enhances the Metastatic Potential of Aggressive Primary Prostate Cancer

Author

Van den Broeck, Thomas, primary, Moris, Lisa, primary, Gevaert, Thomas, primary, Davicioni, Elai, primary, Boeckx, Bram, primary, Lambrechts, Diether, primary, Helsen, Christine, primary, Handle, Florian, primary, Ghesquière, Bart, primary, Soenen, Stefaan, primary, Smeets, Elien, primary, Eerlings, Roy, primary, El Kharraz, Sarah, primary, Devlies, Wout, primary, Karnes, R. Jeffrey, primary, Lotan, Tamara, primary, Van Poppel, Hendrik, primary, Joniau, Steven, primary, and Claessens, Frank, primary

Published
2023
Full Text
View/download PDF

12. Supplementary Figure from Antizyme Inhibitor 1 Regulates Matrikine Expression and Enhances the Metastatic Potential of Aggressive Primary Prostate Cancer

Author

Van den Broeck, Thomas, primary, Moris, Lisa, primary, Gevaert, Thomas, primary, Davicioni, Elai, primary, Boeckx, Bram, primary, Lambrechts, Diether, primary, Helsen, Christine, primary, Handle, Florian, primary, Ghesquière, Bart, primary, Soenen, Stefaan, primary, Smeets, Elien, primary, Eerlings, Roy, primary, El Kharraz, Sarah, primary, Devlies, Wout, primary, Karnes, R. Jeffrey, primary, Lotan, Tamara, primary, Van Poppel, Hendrik, primary, Joniau, Steven, primary, and Claessens, Frank, primary

Published
2023
Full Text
View/download PDF

13. Supplementary Table from Antizyme Inhibitor 1 Regulates Matrikine Expression and Enhances the Metastatic Potential of Aggressive Primary Prostate Cancer

Author

Van den Broeck, Thomas, primary, Moris, Lisa, primary, Gevaert, Thomas, primary, Davicioni, Elai, primary, Boeckx, Bram, primary, Lambrechts, Diether, primary, Helsen, Christine, primary, Handle, Florian, primary, Ghesquière, Bart, primary, Soenen, Stefaan, primary, Smeets, Elien, primary, Eerlings, Roy, primary, El Kharraz, Sarah, primary, Devlies, Wout, primary, Karnes, R. Jeffrey, primary, Lotan, Tamara, primary, Van Poppel, Hendrik, primary, Joniau, Steven, primary, and Claessens, Frank, primary

Published
2023
Full Text
View/download PDF

14. Supplementary methods from The Effect of F877L and T878A Mutations on Androgen Receptor Response to Enzalutamide

Author

Prekovic, Stefan, primary, van Royen, Martin E., primary, Voet, Arnout R.D., primary, Geverts, Bart, primary, Houtman, Rene, primary, Melchers, Diana, primary, Zhang, Kam Y.J., primary, Van den Broeck, Thomas, primary, Smeets, Elien, primary, Spans, Lien, primary, Houtsmuller, Adriaan B., primary, Joniau, Steven, primary, Claessens, Frank, primary, and Helsen, Christine, primary

Published
2023
Full Text
View/download PDF

15. RF03 | PMON286 Engineering Steroid Receptors to Respond to Chemicals of Choice

Author

Claessens, Frank, primary, Eerlings, Roy, additional, Gupta, Purvi, additional, Lee, Xiao Yin, additional, Kharraz, Sarah E, additional, Jacquemyn, Maarten, additional, Vandewinkel, Bram, additional, Munter, Sofie D, additional, Nguyen, Tien, additional, Handle, Florian, additional, Smeets, Elien, additional, Moris, Lisa, additional, Devlies, Wout, additional, Voordeckers, Karin, additional, Gorkovskiy, Anton, additional, Henckaerts, Els, additional, Daelemans, Dirk, additional, Verstrepen, Kevin J, additional, Voet, Arnout, additional, and Helsen, Christine, additional

Published
2022
Full Text
View/download PDF

16. N/C Interactions Are Dispensable for Normal In Vivo Functioning of the Androgen Receptor in Male Mice

Author

El Kharraz, Sarah, primary, Dubois, Vanessa, additional, Launonen, Kaisa-Mari, additional, Helminen, Laura, additional, Palvimo, Jorma J, additional, Libert, Claude, additional, Smeets, Elien, additional, Moris, Lisa, additional, Eerlings, Roy, additional, Vanderschueren, Dirk, additional, Helsen, Christine, additional, and Claessens, Frank, additional

Published
2022
Full Text
View/download PDF

17. A disease‐associated missense mutation in CYP4F3 affects the metabolism of leukotriene B4 via disruption of electron transfer

Author

Smeets, Elien, primary, Huang, Shengyun, additional, Lee, Xiao Yin, additional, Van Nieuwenhove, Erika, additional, Helsen, Christine, additional, Handle, Florian, additional, Moris, Lisa, additional, El Kharraz, Sarah, additional, Eerlings, Roy, additional, Devlies, Wout, additional, Willemsen, Mathijs, additional, Bücken, Leoni, additional, Prezzemolo, Teresa, additional, Humblet‐Baron, Stephanie, additional, Voet, Arnout, additional, Rochtus, Anne, additional, Van Schepdael, Ann, additional, de Zegher, Francis, additional, and Claessens, Frank, additional

Published
2022
Full Text
View/download PDF

18. Antizyme Inhibitor 1 regulates matrikine expression and enhances the metastatic potential of aggressive primary prostate cancer

Author

Van den Broeck, Thomas, primary, Moris, Lisa, additional, Gevaert, Thomas, additional, Davicioni, Elai, additional, Boeckx, Bram, additional, Lambrechts, Diether, additional, Helsen, Christine, additional, Handle, Florian, additional, Ghesquiere, Bart, additional, Soenen, Stefaan, additional, Smeets, Elien, additional, Eerlings, Roy, additional, El Kharraz, Sarah, additional, Devlies, Wout, additional, Karnes, R. Jeffrey, additional, Lotan, Tamara, additional, Van Poppel, Hendrik, additional, Joniau, Steven, additional, and Claessens, Frank, additional

Published
2022
Full Text
View/download PDF

19. N/C Interactions Are Dispensable for Normal In Vivo Functioning of the Androgen Receptor in Male Mice.

Author

Kharraz, Sarah El, Dubois, Vanessa, Launonen, Kaisa-Mari, Helminen, Laura, Palvimo, Jorma J, Libert, Claude, Smeets, Elien, Moris, Lisa, Eerlings, Roy, Vanderschueren, Dirk, Helsen, Christine, and Claessens, Frank

Subjects
ANDROGEN receptors, PHENOTYPES
Abstract

The androgen receptor (AR) plays a central role in the development and maintenance of the male phenotype. The binding of androgens to the receptor induces interactions between the carboxyterminal ligand-binding domain and the highly conserved 23FQNLF27 motif in the aminoterminal domain. The role of these so-called N/C interactions in AR functioning is debated. In vitro assays show that mutating the AR in the 23FQNLF27 motif (called ARNoC) attenuates the AR transactivation of reporter genes, has no effect on ligand binding, but does affect protein-protein interactions with several AR coregulators. To test the in vivo relevance of the N/C interaction, we analyzed the consequences of the genomic introduction of the ARNoC mutation in mice. Surprisingly, the ARNoC/Y mice show a normal male development, with unaffected male anogenital distance and normal accessory sex glands, male circulating androgen levels, body composition, and fertility. The responsiveness of androgen target genes in kidney, prostate, and testes was also unaffected. We thus conclude that the N/C interactions in the AR are not essential for the development of a male phenotype under normal physiological conditions. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

20. Abstract B065: Genomic analysis of localized prostate cancer identifies AZIN1 as driver of metastatic progression

Author

Broeck, Thomas Van den, primary, Moris, Lisa, additional, Gevaert, Thomas, additional, Smeets, Elien, additional, Prekovic, Stefan, additional, Helsen, Christine, additional, Poppel, Hendrik van, additional, Everaerts, Wouter, additional, Buerki, Christine, additional, Davicioni, Elai, additional, Joniau, Steven, additional, and Claessens, Frank, additional

Published
2018
Full Text
View/download PDF

22. Metastases-prone localized prostate cancer: a genomic analysis

Author

Van, den Broeck Thomas, primary, Gevaert, Thomas, additional, Prekovic, Stefan, additional, Boeckx, Bram, additional, Smeets, Elien, additional, Ong, Kaye, additional, Lehrer, Jonathan, additional, Haddad, Zaid, additional, Erho, Nicholas, additional, Helsen, Christine, additional, Lambrechts, Diether, additional, Buerki, Christine, additional, Davicioni, Elai, additional, Joniau, Steven, additional, and Claessens, Frank, additional

Published
2016
Full Text
View/download PDF

23. Genomic analysis of Enzalutamide-resistant cells

Author

Prekovic, Stefan, primary, Van, den Broeck Thomas, additional, Smeets, Elien, additional, van, Royen Martin, additional, Houtsmuller, Adriaan, additional, Sahu, Biswajyoti, additional, Pihlajamaa, Paivi, additional, Janne, Olli A., additional, Attard, Gerhardt, additional, Claessens, Frank, additional, and Helsen, Christine, additional

Published
2016
Full Text
View/download PDF

24. The Effect of F877L and T878A Mutations on Androgen Receptor Response to Enzalutamide

Author

Prekovic, Stefan, primary, van Royen, Martin E., additional, Voet, Arnout R.D., additional, Geverts, Bart, additional, Houtman, Rene, additional, Melchers, Diana, additional, Zhang, Kam Y.J., additional, Van den Broeck, Thomas, additional, Smeets, Elien, additional, Spans, Lien, additional, Houtsmuller, Adriaan B., additional, Joniau, Steven, additional, Claessens, Frank, additional, and Helsen, Christine, additional

Published
2016
Full Text
View/download PDF

25. Genomic and epigenomic analysis of high-risk prostate cancer reveals changes in hydroxymethylation and TET1

Author

Spans, Lien, primary, Van den Broeck, Thomas, additional, Smeets, Elien, additional, Prekovic, Stefan, additional, Thienpont, Bernard, additional, Lambrechts, Diether, additional, Karnes, R. Jeffrey, additional, Erho, Nicholas, additional, Alshalalfa, Mohammed, additional, Davicioni, Elai, additional, Helsen, Christine, additional, Gevaert, Thomas, additional, Tosco, Lorenzo, additional, Haustermans, Karin, additional, Lerut, Evelyne, additional, Joniau, Steven, additional, and Claessens, Frank, additional

Published
2016
Full Text
View/download PDF

26. Rational evolution for altering the ligand preference of estrogen receptor alpha.

Author

Eerlings R, Gupta P, Lee XY, Nguyen T, El Kharraz S, Handle F, Smeets E, Moris L, Devlies W, Vandewinkel B, Thiry I, Ta DT, Gorkovskiy A, Voordeckers K, Henckaerts E, Pinheiro VB, Claessens F, Verstrepen KJ, Voet A, and Helsen C

Subjects
Animals, Mice, Ligands, Tamoxifen pharmacology, Tamoxifen metabolism, Receptors, Estrogen genetics, Receptors, Estrogen chemistry, Receptors, Estrogen metabolism, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Mammals, Estrogen Receptor alpha genetics, Estrogen Receptor alpha chemistry, Estrogen Receptor alpha metabolism, Estradiol chemistry, Estradiol metabolism
Abstract

Estrogen receptor α is commonly used in synthetic biology to control the activity of genome editing tools. The activating ligands, estrogens, however, interfere with various cellular processes, thereby limiting the applicability of this receptor. Altering its ligand preference to chemicals of choice solves this hurdle but requires adaptation of unspecified ligand-interacting residues. Here, we provide a solution by combining rational protein design with multi-site-directed mutagenesis and directed evolution of stably integrated variants in Saccharomyces cerevisiae. This method yielded an estrogen receptor variant, named TERRA, that lost its estrogen responsiveness and became activated by tamoxifen, an anti-estrogenic drug used for breast cancer treatment. This tamoxifen preference of TERRA was maintained in mammalian cells and mice, even when fused to Cre recombinase, expanding the mammalian synthetic biology toolbox. Not only is our platform transferable to engineer ligand preference of any steroid receptor, it can also profile drug-resistance landscapes for steroid receptor-targeted therapies., (© 2024 The Protein Society.)

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results