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Neuropsychiatric disorders that result from stress exposure are highly associated with central inflammation. Our previous work established that females selectively exhibit heightened proinflammatory cytokine production within the noradrenergic locus coeruleus (LC) along with a hypervigilant behavioral phenotype in response to witnessing social stress, and ablation of microglia using pharmacological techniques prevents this behavioral response. These studies were designed to further investigate the impact of stress-induced neuroimmune signaling on the long-term behavioral and neuronal consequences of social stress exposure in females using chemogenetics. We first characterized the use of an AAV-CD68-G i -DREADD virus targeted to microglia within the LC and confirmed viral transduction, selectivity, and efficacy. Clozapine-n-oxide (CNO) was used for the suppression of microglial reactivity during acute and chronic exposure to vicarious/witness social defeat in female rats. Chemogenetic-mediated inhibition of microglial reactivity during stress blunted the neuroimmune response to stress and prevented both acute and long-term hypervigilant behavioral responses. Further, a history of microglial suppression during stress prevented the heightened LC activity typically observed in response to stress cues. These studies are among the first to use a chemogenetic approach to inhibit microglia within the female brain in vivo and establish LC inflammation as a key mechanism underlying the behavioral and neuronal responses to social stress in females., Competing Interests: Conflicts of Interest The authors have no conflicts of interest to report.

Undergraduate neuroscience researchers and educators have a vital voice in working with policymakers to raise public awareness and increase support and funding for neuroscience. While there are many avenues and opportunities to become involved in neuroscience advocacy, finding the most effective training strategies, resources, and opportunities for involvement can sometimes be difficult and overwhelming. To address this challenge and inform faculty of science advocacy opportunities for undergraduates, we organized a mini-symposium at the 2023 Faculty for Undergraduate Neuroscience (FUN) Workshop. Attendees had the opportunity to engage with a panel of experts with diverse experiences in neuroscience advocacy and policy. Topics presented and discussed included the importance of advocacy, effective training practices and resources, advice for scientific communication with a non-scientific audience, and various opportunities for advocacy involvement for undergraduate students. We share here our rationale and goals as we set out to plan this mini-symposium, a brief description of each panelist's career trajectory, relevant resources, and major takeaways. We reflect on the lessons learned from this session and recognize the need for an on-going conversation about careers involving science policy, science communication training, and opportunities for undergraduate students. Accordingly, we share future directions and recommendations to help faculty equip not only themselves but also their undergraduate trainees with the knowledge, practical skills, and resources required to engage with their communities as informed citizens., (Copyright © 2024 Faculty for Undergraduate Neuroscience.)