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Devising an approach to deterministically position organisms can impact various fields such as bioimaging, cybernetics, cryopreservation, and organism‐integrated devices. This requires continuously assessing the locations of randomly distributed organisms to collect and transfer them to target spaces without harm. Here, an aspiration‐assisted adaptive printing system is developed that tracks, harvests, and relocates living and moving organisms on target spaces via a pick‐and‐place mechanism that continuously adapts to updated visual and spatial information about the organisms and target spaces. These adaptive printing strategies successfully positioned a single static organism, multiple organisms in droplets, and a single moving organism on target spaces. Their capabilities are exemplified by printing vitrification‐ready organisms in cryoprotectant droplets, sorting live organisms from dead ones, positioning organisms on curved surfaces, organizing organism‐powered displays, and integrating organisms with materials and devices in customizable shapes. These printing strategies can ultimately lead to autonomous biomanufacturing methods to evaluate and assemble organisms for a variety of single and multi‐organism‐based applications. [ABSTRACT FROM AUTHOR]

Objective: We propose and assess galvanic vestibular stimulation (GVS) as a novel means to provide information dissociated from self-orientation. Background: In modern user interfaces, visual and auditory modalities dominate information transfer so much that these "processing channels" become overloaded with information. Fortunately, the brain is capable of processing separate sensory sources in parallel enabling alternative display modalities to inform operators more effectively and without increasing cognitive strain. To date, the vestibular system, normally responsible for sensing self-orientation and helping with balance, has not been considered as a display modality. Method: Bilateral GVS was provided at 0.6 mA for 1-second intervals with moderately high-frequency sinusoidal waveforms, designed to not elicit sensations of self-motion. We assessed subjects' ability to differentiate between two cues of different frequencies. Results: We found subjects were able to reliably distinguish between cues with an average just-noticeable difference threshold of only ±12 Hz (range across subjects: 5.4–19.6 Hz) relative to a pedestal cue of 50 Hz. Further, we found the GVS sensory modality to be robust to various environments: walking, standing, sitting, passive motion, and loud background noise. Finally, the application of the GVS cues did not have significant destabilizing effects when standing or walking. Conclusion: These results show that GVS may be an effective alternative display modality, using varying frequency to encode information. It is robust to various operational environments and non-destabilizing. Application: A fully functional display can convey information to operators of vehicles and other machinery as well as high-performance operators like astronauts and soldiers. [ABSTRACT FROM AUTHOR]

Vitrification‐based cryopreservation is a promising approach to achieving long‐term storage of biological systems for maintaining biodiversity, healthcare, and sustainable food production. Using the "cryomesh" system achieves rapid cooling and rewarming of biomaterials, but further improvement in cooling rates is needed to increase biosystem viability and the ability to cryopreserve new biosystems. Improved cooling rates and viability are possible by enabling conductive cooling through cryomesh. Conduction‐dominated cryomesh improves cooling rates from twofold to tenfold (i.e., 0.24 to 1.2 × 105 °C min−1) in a variety of biosystems. Higher thermal conductivity, smaller mesh wire diameter and pore size, and minimizing the nitrogen vapor barrier (e.g., vertical plunging in liquid nitrogen) are key parameters to achieving improved vitrification. Conduction‐dominated cryomesh successfully vitrifies coral larvae, Drosophila embryos, and zebrafish embryos with improved outcomes. Not only a theoretical foundation for improved vitrification in µm to mm biosystems but also the capability to scale up for biorepositories and/or agricultural, aquaculture, or scientific use are demonstrated. [ABSTRACT FROM AUTHOR]

Purpose: This study examined whether a higher protein diet following strenuous exercise can alter markers of muscle damage and inflammation in older adults. Methods: Using a double-blind, independent group design, 10 males and eight females (age 57 ± 4 years; mass 72.3 ± 5.6 kg; height 1.7 ± 6.5 m) were supplied with a higher protein (2.50 g·kg−1·day−1) or moderate protein (1.25 g·kg−1·day−1) diet for 48 hr after 140 squats with 25% of their body mass. Maximal isometric voluntary contractions, muscle soreness, creatine kinase, Brief Assessment of Mood Adapted, and inflammatory markers were measured preexercise, and 24 hr and 48 hr postexercise. Results: The maximal isometric voluntary contractions decreased postexercise (p = .001, η2p = .421), but did not differ between groups (p = .822, η2p = .012). Muscle soreness peaked at 24 hr post in moderate protein (44 ± 30 mm) and 48 hr post in higher protein (70 ± 46 mm; p = .005; η2p = .282); however, no group differences were found (p = .585; η2p = .083). Monocytes and lymphocytes significantly decreased postexercise, and eosinophils increased 24 hr postexercise (p < 0.05), but neutrophils, creatine kinase, interleukin-6, C-reactive protein, monocyte chemotactic protein-1, and Brief Assessment of Mood Adapted were unchanged by exercise or the intervention (p > .05). Conclusion: In conclusion, 2.50 g·kg−1·day−1 of protein is not more effective than 1.25 g·kg−1·day−1 for attenuating indirect markers of muscle damage and inflammation following strenuous exercise in older adults. [ABSTRACT FROM AUTHOR]

We report Zeeman infra-red spectroscopy of electronic-nuclear levels of 5I8 → 5I7 transitions of Ho3+ in the C4v(F–) centre in CaF2 with the magnetic field along the 〈111〉 direction of the crystal. Transitions to the lowest 5I7 state, an isolated electronic doublet, and the next group of states, a pseudo-quadruplet consisting of a doublet and two nearby singlets, exhibit strongly non-linear Zeeman splittings and intensity variations. Simulated spectra based upon a crystal-field analysis give an excellent approximation to the data, illustrating the strong predictive ability of the parametrized crystal-field approach. Anti-crossings in the hyperfine splittings, the basis of quantum information storage in rare-earth doped insulating dielectrics, are also predicted. [ABSTRACT FROM AUTHOR]

We report Zeeman infra-red spectroscopy of electronic-nuclear levels of 5I8 → 5I7 transitions of Ho3+ in the C4v(F–) centre in CaF2 with the magnetic field along the 〈111〉 direction of the crystal. Transitions to the lowest 5I7 state, an isolated electronic doublet, and the next group of states, a pseudo-quadruplet consisting of a doublet and two nearby singlets, exhibit strongly non-linear Zeeman splittings and intensity variations. Simulated spectra based upon a crystal-field analysis give an excellent approximation to the data, illustrating the strong predictive ability of the parametrized crystal-field approach. Anti-crossings in the hyperfine splittings, the basis of quantum information storage in rare-earth doped insulating dielectrics, are also predicted. [ABSTRACT FROM AUTHOR]