1. Outer Electrospun Polycaprolactone Shell Induces Massive Foreign Body Reaction and Impairs Axonal Regeneration through 3D Multichannel Chitosan Nerve Guides
- Author
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Soenke Wienecke, Birgit Glasmacher, Lutz Dreyer, Kirsten Haastert-Talini, Tanmay Chakradeo, Peter Behrens, and Sven Duda
- Subjects
nerve fiber regeneration ,Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie ,muscle ,Nerve guidance conduit ,lcsh:Medicine ,sciatic nerve ,Biocompatible Materials ,Wistar rat ,electrophysiological procedures ,Chitosan ,chemistry.chemical_compound ,rat ,animal ,nociception ,nerve regeneration ,pathophysiology ,automutilation ,Tissue Scaffolds ,adult ,Electrodiagnosis ,Muscles ,tissue scaffold ,biomaterial ,article ,Biomaterial ,organ size ,Pain Perception ,General Medicine ,Anatomy ,Organ Size ,extracellular space ,electric field ,nerve transplantation ,female ,medicine.anatomical_structure ,peripheral nerve ,Polycaprolactone ,chemically induced ,Female ,Sciatic nerve ,scanning electron microscopy ,Research Article ,Article Subject ,Polyesters ,animal experiment ,Nerve fiber ,tissue regeneration ,Motor Activity ,General Biochemistry, Genetics and Molecular Biology ,foreign body reaction ,in vivo study ,polycaprolactone ,ddc:570 ,medicine ,Animals ,polyester ,controlled study ,ddc:610 ,giant cell ,nerve fiber ,procedures ,Rats, Wistar ,electrospinning ,Inflammation ,nonhuman ,General Immunology and Microbiology ,Guided Tissue Regeneration ,Regeneration (biology) ,Foreign-Body Reaction ,lcsh:R ,technology, industry, and agriculture ,convalescence ,Recovery of Function ,equipment and supplies ,microenvironment ,Dewey Decimal Classification::600 | Technik ,Axons ,Nerve Regeneration ,Transplantation ,chemistry ,drug effects ,freeze drying ,Microscopy, Electron, Scanning ,pathology ,Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit ,ddc:600 ,Biomedical engineering - Abstract
We report on the performance of composite nerve grafts with an inner 3D multichannel porous chitosan core and an outer electrospun polycaprolactone shell. The inner chitosan core provided multiple guidance channels for regrowing axons. To analyze thein vivoproperties of the bare chitosan cores, we separately implanted them into an epineural sheath. The effects of both graft types on structural and functional regeneration across a 10 mm rat sciatic nerve gap were compared to autologous nerve transplantation (ANT). The mechanical biomaterial properties and the immunological impact of the grafts were assessed with histological techniques before and after transplantationin vivo. Furthermore during a 13-week examination period functional tests and electrophysiological recordings were performed and supplemented by nerve morphometry. The sheathing of the chitosan core with a polycaprolactone shell induced massive foreign body reaction and impairment of nerve regeneration. Although the isolated novel chitosan core did allow regeneration of axons in a similar size distribution as the ANT, the ANT was superior in terms of functional regeneration. We conclude that an outer polycaprolactone shell should not be used for the purpose of bioartificial nerve grafting, while 3D multichannel porous chitosan cores could be candidate scaffolds for structured nerve grafts.
- Published
- 2014
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