1. Neutrophil elastase-mediated increase in airway temperature during inflammation
- Author
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Baroukh M. Assael, Susanne Häußler, Annika Schmidt, Jutta Hammermann, Monika Schniederjans, Gerd Döring, Rosi Bissinger, Joachim Seelig, Garrit Koller, D. Worlitzsch, Keith C. Meyer, Kenneth D. Bruce, Soeren Damkiaer, Otto Holst, Giorgio Piacentini, John J. LiPuma, Laurette Malleret, Azzaq Belaaouaj, Antonio Molinaro, Ciro D'Orazio, B Schulte-Hubbert, Martino Facchinelli, Schmidt, Annika, Belaaouaj, Azzaq, Bissinger, Rosi, Koller, Garrit, Malleret, Laurette, D'Orazio, Ciro, Facchinelli, Martino, Schulte-Hubbert, Bernhard, Molinaro, Antonio, Holst, Otto, Hammermann, Jutta, Schniederjans, Monika, Meyer, Keith C., Damkiaer, Soeren, Piacentini, Giorgio, Assael, Baroukh, Bruce, Kenneth, Häußler, Susanne, Lipuma, John J., Seelig, Joachim, Worlitzsch, Dieter, Döring, Gerd, Institute of Medical Microbiology and Hygiene (IMHR), Universität Regensburg (UR), Pathogénie des Staphylocoques – Staphylococcal Pathogenesis (StaPath), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), King‘s College London, Department of Pediatrics, Università degli studi di Verona = University of Verona (UNIVR), Ospedale Civile Maggiore, Verona CF center-Università degli studi di Verona = University of Verona (UNIVR), Medical Clinic and Policlinic I, University Hospital Carl Gustav Carus Dresden, Department of Chemical Sciences, University of Naples Federico II = Università degli studi di Napoli Federico II, Research Center Borstel, Leibniz Center for Medicine and Biosciences, University Clinic Carl Gustav Carus, Dresden, Helmholtz Centre for Infection Research (HZI), University of Wisconsin School of Medicine and Public Health, Danmarks Tekniske Universitet = Technical University of Denmark (DTU), University of Michigan Medical School [Ann Arbor], University of Michigan [Ann Arbor], University of Michigan System-University of Michigan System, Departement of Biophysical Chemistry, University of Basel (Unibas), Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), University of Verona (UNIVR), Verona CF center-University of Verona (UNIVR), University of Naples Federico II, Technical University of Denmark [Lyngby] (DTU), Biozentrum Universitaet Basel, École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and University of Verona (UNIVR)-Verona CF center
- Subjects
Male ,Hot Temperature ,Cystic Fibrosis ,Inbred C57BL ,medicine.disease_cause ,Cystic fibrosis ,Body Temperature ,Neutrophil elastase ,Mice ,0302 clinical medicine ,Medicine ,Respiratory Tract Infection ,Child ,Pathogen ,Respiratory Tract Infections ,0303 health sciences ,biology ,Temperature ,3. Good health ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Pseudomonas aeruginosa ,Inflammation ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,Female ,medicine.symptom ,Case-Control Studie ,Human ,Pulmonary and Respiratory Medicine ,Adolescent ,Microbiology ,Pseudomonas Infection ,03 medical and health sciences ,Animals ,Humans ,Pseudomonas Infections ,Pediatrics, Perinatology, and Child Health ,Cystic Fibrosi ,030304 developmental biology ,Innate immune system ,business.industry ,Animal ,medicine.disease ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Mice, Inbred C57BL ,Chronic infection ,Disease Models, Animal ,030228 respiratory system ,Case-Control Studies ,Disease Models ,Immunology ,Pediatrics, Perinatology and Child Health ,biology.protein ,business ,Airway ,Leukocyte Elastase - Abstract
International audience; BACKGROUND: How elevated temperature is generated during airway infections represents a hitherto unresolved physiological question. We hypothesized that innate immune defence mechanisms would increase luminal airway temperature during pulmonary infection. METHODS: We determined the temperature in the exhaled air of cystic fibrosis (CF) patients. To further test our hypothesis, a pouch inflammatory model using neutrophil elastase-deficient mice was employed. Next, the impact of temperature changes on the dominant CF pathogen Pseudomonas aeruginosa growth was tested by plating method and RNAseq. RESULTS: Here we show a temperature of ~38°C in neutrophil-dominated mucus plugs of chronically infected CF patients and implicate neutrophil elastase:α1-proteinase inhibitor complex formation as a relevant mechanism for the local temperature rise. Gene expression of the main pathogen in CF, P. aeruginosa, under anaerobic conditions at 38°C vs 30°C revealed increased virulence traits and characteristic cell wall changes. CONCLUSION: Neutrophil elastase mediates increase in airway temperature, which may contribute to P. aeruginosa selection during the course of chronic infection in CF.
- Published
- 2014
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