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Paralytic shellfish poisoning is a worldwide problem induced by shellfish contaminated with paralytic shellfish toxins. To protect human health, a regulatory limit for these toxins in shellfish flesh has been adopted by many countries. In a recent study, mice were dosed with saxitoxin and tetrodotoxin mixtures daily for 28 days showing toxicity at low concentrations, which appeared to be at odds with other work. To further investigate this reported toxicity, we dosed groups of mice with saxitoxin and tetrodotoxin mixtures daily for 21 days. In contrast to the previous study, no effects on mouse bodyweight, food consumption, heart rate, blood pressure, grip strength, blood chemistry or hematology were observed. Furthermore, no histological findings were associated with dosing in this trial. The dose rates in this study were 2.6, 3.8 and 4.9 times greater, respectively, than the highest dose of the previous study. As rapid mortality in three out of five mice was observed in the previous study, the deaths are likely to be due to the methodology used rather than the shellfish toxins. To convert animal data to that used in a human risk assessment, a 100-fold safety factor is required. After applying this safety factor, the dose rates used in the current study were 3.5, 5.0 and 6.5 times greater, respectively, than the acute reference dose for each toxin type set by the European Union. Furthermore, it has previously been proposed that tetrodotoxin be included in the paralytic shellfish poisoning suite of toxins. If this were done, the highest dose rate used in this study would be 13 times the acute reference dose. This study suggests that the previous 28-day trial was flawed and that the current paralytic shellfish toxin regulatory limit is fit for purpose. An additional study, feeding mice a diet laced with the test compounds at higher concentrations than those of the current experiment, would be required to comment on whether the current paralytic shellfish toxin regulatory limit should be modified.

Aphids are major pests of cereal and pasture grasses throughout the world, vectoring disease and reducing plant production. There are few control options other than insecticides. Epichloë endophytes that produce loline alkaloids in their hosts provide a possible mechanism of control, with both meadow fescue and tall fescue naturally infected with loline-producing endophytes showing a resistance to Rhopalosiphum padi. We screened Elymus spp. naturally infected with endophytes that produced loline alkaloids at concentrations known to affect aphids on fescue but found no effect on these insects infesting Elymus . A synthetic loline-producing endophyte association with rye also had no effect on the aphids. After hypothesizing that the lolines were being translocated in the xylem in Elymus and rye rather than the phloem, we tested the rye and meadow fescue infected with loline-producing endophytes against a xylem feeding spittlebug. The endophyte in rye inhibited the feeding of the insect and reduced its survival, whereas the endophyte-infected meadow fescue had no effect on the spittlebug but reduced the number of aphids. Lolines applied to the potting medium of endophyte-free and endophyte-infected rye, ryegrass, and tall fescue resulted in a decrease in the aphid populations on the endophyte-free pasture grasses relative to the untreated controls but had no effect on aphid numbers on the rye. We tentatively conclude that lolines, produced in both natural and synthetic association with Elymus and rye, are partitioned in the xylem rather than the phloem, where they are inaccessible to aphids.

Early detection of bark- and wood-boring beetles is critical to support the eradication of recently established populations in novel regions. Flight intercept traps baited with semiochemical lures are essential for surveillance and population monitoring of introduced insects. We present laboratory and field data to test potential improvements in trap sensitivity to detect Hylastes ater (Paykull), Hylurgus ligniperda (Fabricius) (Coleoptera:Scolytinae), and Arhopalus ferus (Mulsant) (Coleoptera:Cerambycidae). We evaluated the effects of type and permeability of plastic material on release rates of host-plant volatiles and the addition of the pheromone racemic E-fuscumol on beetle species catch within an exotic Pinus radiata (D. Don) plantation forest. We trapped 228,278 H. ligniperda, 91 H. ater, and 2,504 A. ferus over two experiments. Our results show strong attraction of H. ligniperda to host-plant volatiles with a positive relationship between increased release rates and trap catch. H. ater follows this trend, with attraction to ethanol and (-)-β-pinene. H. ligniperda was more strongly attracted to host volatiles than H. ater, the average ratio between baited and unbaited traps being 51.25:1 and 3.75:1, respectively for each species. There was no effect of host-plant volatiles or release rate on A. ferus catch, but a weak attraction by A. ferus to racemic E-fuscumol. Overall, we demonstrate species-specific differences in Coleoptera attraction to host-plant volatile release rates and semiochemical blends. Results demonstrate mechanisms to improve trap catch of H. ligniperda and H. ater. Racemic E-fuscumol shows some promise for A. ferus, but further investigation is required to determine whether chirality affects attraction., (© The Author(s) 2021. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Background: Although unmanned aerial vehicles (UAVs) are increasingly used to deliver small-scale aerial pesticide applications, there remains uncertainty over their efficiency in terms of uniformity of spray deposition and their application efficiency. Consequently, a field study was designed to quantify factors influencing the uniformity of spray deposition from a multi-rotor UAV that is operated commercially in New Zealand. Two sampling systems for measuring spray deposition, a continuous horizontal string and steel plates placed on the ground were compared., Results: The spray deposit distribution characteristics and calculated lane separation values (distance between flight lines that produces a maximum coefficient of variation of 30% for spray deposits) were strongly influenced by wind speed, nozzle position, release height, ground speed and droplet size. Lane separation values ranged from 1 to > 5 m. Swath distribution parameters (spread and position of peak deposition) derived from plates were not significantly different from those derived from string. However, total deposition on strings relative to plates increased with small droplets. Reducing plate sampling intensity from 0.25 m intervals to 0.5 m and even 1.0 m had only a minor effect on estimates of swath parameter values., Conclusions: The potential for precision spraying from UAV platforms has yet to be achieved with improvements required in hardware and software. Further, mechanistic models are needed to quantify how complex interactions among multiple operating and meteorological variables influence spray deposition. © 2019 Society of Chemical Industry., (© 2019 Society of Chemical Industry.)

Red needle cast is a significant foliar disease of commercial stands of Pinus radiata caused by Phytophthora pluvialis in New Zealand. The effect of copper, applied as a foliar spray of cuprous oxide at a range of doses between 0 and 1.72 kg ha -1 , was investigated in two controlled trials with potted plants and in an operational trial with mature P. radiata . In all trials, lesions formed on needles after artificial exposure to the infecting propagules (zoospores) of P. pluvialis were used to determine treatment efficacy, with the number and/or length of lesions as the dependent variable. Results across all trials indicated that cuprous oxide was highly effective at reducing infection of P. radiata with P. pluvialis . Application rates equivalent to ≥0.65 kg ha -1 significantly reduced infection levels relative to a control treatment, with foliar surface copper levels as low as 13 to 26 mg kg -1 of needle tissue preventing infection. Greater copper content was associated with a reduction in the proportion of needles with P. pluvialis lesions, with the probability of lesions developing decreasing approximately 1% for every 1 unit (in milligrams per kilogram) increase in copper content. Over a 90-day period, surface copper content declined to 30% of that originally applied, indicating an approximate period of treatment efficacy of 3 months. Our findings highlight the potential of cuprous oxide for the control of red needle cast in P. radiata stands. Further information about the optimal field dose, timing, and the frequency of foliar cuprous oxide application is key to prevent infection and also reduce the build up of inoculum during severe outbreaks of this pathogen.