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Background: Ovarian cancer, as a highly malignant tumor, features the critical involvement of tumor-associated fibroblasts in the ovarian cancer tissue microenvironment. However, due to the apparent heterogeneity within fibroblast subpopulations, the specific functions of these subpopulations in the ovarian cancer tissue microenvironment remain insufficiently elucidated. Methods: In this study, we integrated single-cell sequencing data from 32 ovarian cancer samples derived from four distinct cohorts and 3226 bulk RNA-seq data from GEO and TCGA-OV cohorts. Utilizing computational frameworks such as Seurat, Monocle 2, Cellchat, and others, we analyzed the characteristics of the ovarian cancer tissue microenvironment, focusing particularly on fibroblast subpopulations and their differentiation trajectories. Employing the CIBERSORTX computational framework, we assessed various cellular components within the ovarian cancer tissue microenvironment and evaluated their associations with ovarian cancer prognosis. Additionally, we conducted Mendelian randomization analysis based on cis-eQTL to investigate causal relationships between gene expression and ovarian cancer. Results: Through integrative analysis, we identified 13 major cell types present in ovarian cancer tissues, including CD8+ T cells, malignant cells, and fibroblasts. Analysis of the tumor microenvironment (TME) cell proportions revealed a significant increase in the proportion of CD8+ T cells and CD4+ T cells in tumor tissues compared to normal tissues, while fibroblasts predominated in normal tissues. Further subgroup analysis of fibroblasts identified seven subgroups, with the MMP11+Fib subgroup showing the highest activity in the TGFβ signaling pathway. Single-cell analysis suggested that oxidative phosphorylation could be a key pathway driving fibroblast differentiation, and the ATRNL1+KCN + Fib subgroup exhibited chromosomal copy number variations. Prognostic analysis using a large sample size indicated that high infiltration of MMP11+ fibroblasts was associated with poor prognosis in ovarian cancer. SMR analysis identified 132 fibroblast differentiation-related genes, which were linked to pathways such as platinum drug resistance. Conclusions: In the context of ovarian cancer, fibroblasts expressing MMP11 emerge as the primary drivers of the TGF-beta signaling pathway. Their presence correlates with an increased risk of adverse ovarian prognoses. Additionally, the genetic regulation governing the differentiation of fibroblasts associated with ovarian cancer correlates with the emergence of drug resistance.

The corrosion behavior of hot air solder level printed circuit boards(PCB-HASL) and electroless nickel immersion gold printed circuit boards(PCB-ENIG) in salt spray test was studied by electrochemical impedance spectroscopy. The corrosion product morphology,composition and coating failure mechanism were observed by stereo microscopy,scanning electron microscopy and energy-dispersive X-ray spectroscopy. The results show that PCB-HASL and PCB-ENIG suffer severe corrosion in high salt spray environment. Localized corrosion occurs first in the Sn plating layer,and then almost entire surface corrosion occurs,similar to uniform corrosion. PCB-ENIG mainly generates microporous corrosion. In the PCB-HASL corrosion process,Cl- promotes the corrosion of the Sn layer,then a large amount of corrosion product gradually forms and covers the Sn surface,it makes the corrosion rate reduce.In the PCB-ENIG corrosion process,a thin liquid layer containing Cl- electrolyte forms in micropores,and galvanic coupling between gold and nickel constitutes,which accelerates Ni corrosion. The Cu base directly exposes in the adverse environment after Ni suffers serious corrosion,finally leading to electronic component failure.

Stable isotope techniques provide a new approach to study soil water movement. The precipitation and the soil water from 0 to 100 cm soil layer in 4 kinds of typical vegetation types (forest, shrub forest, grassland and non-forest land) over the water source area of Yuanyang terrace were sampled, and their isotope compositions were analyzed, aimed to understand the characteristics of stable isotopes in different depth of the soil water. The results showed that the meteoric water line in the water source area of Yuanyang terrace was δD = 6.838 4δ(18)O-5.6921 (R2 = 0.8787, n = 20), the slope and intercept were less than the global atmospheric precipitation. The hydrogen and oxygen stable isotopes in the soil water of the 4 kinds of typical types was lower than the local meteoric water line side and the fluctuation of isotope value on surface soil profile was greater. With the increasing soil depth, the fluctuation of delta 18O value was smaller and smaller, especially in the 80-100 cm soil layer which was the most obvious. The delta 18O values of the deep soil water in forest and grassland were higher than that in the surface soil. while it was on the contrary in shrub forest and non-forest land.

Objective: To investigate the relationship between serum concentration of fluorouracil and therapeutic efficacy as well as adverse reactions in patients with unresectable locally advanced or measurable metastatic colorectal cancer, and to analyze its role in further improving therapeutic efficacy and reducing adverse reactions of fluorouracil-based chemotherapy., Methods: Eighty-six patients were randomly assigned into three groups according to the average plasma concentration of fluorouracil after three cycles of chemotherapy with the initial regimen of two weeks FOLFOX-4 (oxaliplatin + leucovorin + fluorouracil) or FOLFIRI (irinotecan + leucovorin + fluorouracil): group 1 (plasma concentration of fluorouracil < 25 ng/ml), group 2 (25 - 35 ng/ml) and group 3 (> 35 ng/ml). The blood samples were taken at 12 h after continuous infusion of fluorouracil in each cycle and the plasma concentration of fluorouracil was detected by high performance liquid chromatography (HPLC) (about 5 am ± 1 h). The relationship between the drug plasma concentration, therapeutic efficacy and adverse reactions in different fluorouracil plasma concentration arms was analyzed retrospectively., Results: The average plasma concentrations of fluorouracil of the three groups were (23.48 ± 1.95) ng/ml, (31.47 ± 2.33) ng/ml and (39.89 ± 3.87) ng/ml, respectively (P < 0.01). As for therapeutic efficacy, the median OS of the groups 2 and 3 were 18.0 and 17.5 months, significantly higher than that in the group 1 (13.0 months, P < 0.01). The PFS were 4.5, 7.5 and 8.0 months, respectively (P < 0.01). In terms of adverse reactions, the incidences of bone marrow suppression, mucositis and diarrhea in the group 3 were significantly higher than that in the first two groups (P = 0.02, P = 0.04 and P = 0.02)., Conclusions: The patients with local advanced and metastatic colorectal cancer, receiving fluorouracil-based chemotherapy, and with an average plasma concentration of fluorouracil between 25 - 35 mg/L have a better prognosis, and lower incidence of adverse reactions such as bone marrow suppression, mucositis and diarrhea.

Objective: To analyze the chemical compositions of Dalbergia odorifera essential oils extacted by CO2-supercritical-fluid-extraction (SFE-CO2) and steam distillation extraction (SD)., Methods: The essential oils of Dalbergia odorifera were extracted by steam distillation extraction and SFE-CO2. The chemical components were separated and analyzed by gas chromatography-mass spectrometry., Results: 12 compounds were identified in SFE sample. The major components from essential oils were 2-propenoic acid-3(4-methoxyphenyl)-ethyl ester (14.53%), nerolidol (14.95%), ageratochromene (1.33%). 9 compounds were identified in SD sample. The major components from essential oils were nerolidol (26.61%), cedrol (1.65%)., Conclusion: The SFE method is better than the SD method in reliability stability and reproducibility, and suitable for essential oils extraction of Dalbergia odorifera.

The main mol-ecule of the title compound, C(45)H(30)N(6)·CH(3)OH, has a non-planar core: the dihedral angles between the benzimidazole rings and the central benzene ring are 20.19 (10), 34.57 (8), and 44.59 (8)°, while the dihedral angles between the peripheral phenyl rings and the attached benzimidazole rings are 84.57 (7), 62.71 (6) and 51.73 (6)°. The tri-substituted benzene mol-ecule is connected to the methanol solvent mol-ecule through an O-H⋯N hydrogen bond, forming a 1:1 solvate. In the crystal structure, no significant π-π inter-actions are present, and the mol-ecules are associated through weak C-H⋯N and C-H⋯O(methanol) contacts.