146 results on '"Songmei Geng"'
Search Results
2. Tildrakizumab for moderate-to-severe plaque psoriasis in Chinese patients: A 12-week randomized placebo-controlled phase III trial with long-term extension
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Chen Yu, Songmei Geng, Bin Yang, Yunhua Deng, Fuqiu Li, Xiaojing Kang, Mingye Bi, Furen Zhang, Yi Zhao, Weili Pan, Zhongwei Tian, Jinhua Xu, Zhenghua Zhang, Nan Yu, Xinsuo Duan, Shuping Guo, Qing Sun, Weiquan Li, Juan Tao, Zhijun Liu, Yuanyuan Yin, Gang Wang, and Lishao Guo
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Medicine - Abstract
Abstract. Background:. There is a need for effective and safe therapies for psoriasis that provide sustained benefits. The aim of this study was to assess the efficacy and safety of tildrakizumab, an anti-interleukin-23p19 monoclonal antibody, for treating moderate-to-severe plaque psoriasis in Chinese patients. Methods:. In this multi-center, double-blind, phase III trial, patients with moderate-to-severe plaque psoriasis were enrolled and randomly assigned (1:1) to receive subcutaneous tildrakizumab 100 mg or placebo at weeks 0 and 4. Patients initially assigned to placebo were switched to receive tildrakizumab at weeks 12, 16, and every 12 weeks thereafter. Patients in the tildrakizumab group continued with tildrakizumab at week 16, and every 12 weeks until week 52. The primary endpoint was the Psoriasis Area and Severity Index (PASI 75) response rate at week 12. Results:. At week 12, tildrakizumab demonstrated significantly higher PASI 75 response rates (66.4% [73/110] vs. 12.7% [14/110]; difference, 51.4% [95% confidence interval (CI), 40.72, 62.13]; P
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- 2024
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3. Efficacy and safety of bilastine vs. levocetirizine for the treatment of chronic idiopathic urticaria: A multicenter, double-blind, double-dummy, phase III, non-inferiority, randomized clinical trial
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Xue Chen, Xiuping Han, Bo Cheng, Hui Li, Lingling Liu, Songmei Geng, Linfeng Li, Jie Li, Qing Guo, Guoyi Zhang, Hong Fang, Jianzhong Zhang, and Lishao Guo
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Medicine - Published
- 2024
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4. Decrease of 5-hydroxymethylcytosine in primary cutaneous CD4+ small/medium sized pleomorphic T-cell lymphoproliferative disorder
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Jiahui Hu, Xinyue Zhang, Lihong Zhao, Qiang Zhao, and Songmei Geng
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Cutaneous T-cell lymphoma ,DNA methylation ,Epigenomics ,Dermatology ,RL1-803 - Abstract
Abstract Background: Primary cutaneous CD4+ small/medium-sized pleomorphic T-Cell lymphoproliferative disorder (PC-SMTLD) has been considered as a controversial dermatological disease that has been included in cutaneous T-cell lymphoma group, presenting most commonly as a solitary nodule and/or plaque with a specific and characteristic head and neck predilection. Due to the considerable overlap between PC-SMTLD and pseudolymphoma (PL), the differential diagnosis is often challenging. Methylation of DNA at position 5 of cytosine, and the subsequent reduction in intracellular 5-hydroxymethylcytosine (5-hmC) levels, is a key epigenetic event in several cancers, including systemic lymphomas. However, it has rarely been studied in cutaneous lymphomas. Objectives: The authors aimed to explore the role of differential 5-hmC immunostaining as a useful marker to distinguish PC-SMTLD from PL. Methods: Retrospective case series study with immunohistochemical and immunofluorescence analysis of 5-hmC was performed in PL and PC-SMTLD. Results: Significant decrease of 5-hmC nuclear staining was observed in PC-SMTLD when compared with PL (p
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- 2024
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5. The impact of smoking and alcohol consumption on rosacea: a multivariable Mendelian randomization study
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Zhaowei Chu, Mengyao Yi, Cong Yan, Bingjie Li, Huan Zhang, Kun Guo, and Songmei Geng
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smoking ,alcohol ,rosacea ,Mendelian randomization ,causality ,Public aspects of medicine ,RA1-1270 - Abstract
BackgroundsObservational studies have shown that cigarette smoking is inversely associated with risk of rosacea, However, it remains uncertain whether this association is causal or it is a result of reverse causation, and whether this association is affected by drinking behaviors.MethodsThis study utilized the summary-level data from the largest genome-wide association study (GWAS) for smoking, alcohol consumption, and rosacea. The objective was to investigate the effect of genetically predicted exposures to smoking and alcohol consumption on the risk of developing rosacea. Two-sample bidirectional Mendelian randomization (MR) was applied, accompanied by sensitive analyses to validate the robustness of findings. Furthermore, multivariable MR was conducted to evaluate the direct impact of smoking on rosacea.ResultsA decreased risk of rosacea was observed in individuals with genetically predicted lifetime smoking [odds ratio (OR)MR − IVW = 0.53; 95% confidence interval (CI), 0.318–0.897; P = 0.017], and number of cigarettes per day (ORMR − IVW = 0.55; 95% CI, 0.358–0.845; P = 0.006). However, no significant associations were found between initiation of regular smoking, smoking cessation, smoking initiation, alcohol consumption and rosacea. Reverse MR analysis did not show any associations between genetic liability toward rosacea and smoking or alcohol drinking. Importantly, the effect of lifetime smoking and the number of cigarettes per day on rosacea remained significant even after adjusting for alcohol consumption in multivariable MR analysis.ConclusionSmoking was causally related to a lower risk of rosacea, while alcohol consumption does not appear to be associated with risk of rosacea.
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- 2024
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6. Hydrogel dressing integrating FAK inhibition and ROS scavenging for mechano-chemical treatment of atopic dermatitis
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Yuanbo Jia, Jiahui Hu, Keli An, Qiang Zhao, Yang Dang, Hao Liu, Zhao Wei, Songmei Geng, and Feng Xu
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Science - Abstract
Abstract Atopic dermatitis (AD) is a chronic skin disease caused by skin immune dyshomeostasis and accompanied by severe pruritus. Although oxidative stress and mechanical scratching can aggravate AD inflammation, treatment targeting scratching is often overlooked, and the efficiency of mechano-chemically synergistic therapy remains unclear. Here, we find that enhanced phosphorylation of focal adhesion kinase (FAK) is associated with scratch-exacerbated AD. We then develop a multifunctional hydrogel dressing that integrates oxidative stress modulation with FAK inhibition to synergistically treat AD. We show that the adhesive, self-healing and antimicrobial hydrogel is suitable for the unique scratching and bacterial environment of AD skin. We demonstrate that it can scavenge intracellular reactive oxygen species and reduce mechanically induced intercellular junction deficiency and inflammation. Furthermore, in mouse AD models with controlled scratching, we find that the hydrogel alleviates AD symptoms, rebuilds the skin barrier, and inhibits inflammation. These results suggest that the hydrogel integrating reactive oxygen species scavenging and FAK inhibition could serve as a promising skin dressing for synergistic AD treatment.
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- 2023
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7. Successful secukinumab therapy in plaque psoriasis is associated with altered gut microbiota and related functional changes
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Xueshan Du, Cong Yan, Shuzhen Kong, Delu Che, Bin Peng, Longfei Zhu, Songmei Geng, and Kun Guo
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secukinumab ,gut microbiota ,psoriasis ,16S rRNA gene sequencing ,shotgun metagenomic analysis ,Microbiology ,QR1-502 - Abstract
IntroductionThe role of gut microbiome dysbiosis in the pathogenesis of psoriasis has gained increasing attention in recent years. Secukinumab, targeting interleukin (IL)-17, has a promising efficacy in psoriasis treatment. However, it remains unclear the gut microbiota alteration and related functional changes caused by successful secukinumab therapy in psoriatic patients.MethodsIn our study, we compared the fecal microbiome profile between psoriatic patients after secukinumab successful treatment (AT) and the other two groups, psoriatic patients without therapy (BT) and healthy people (H), respectively, by using next-generation sequencing targeting 16S ribosomal RNA. Then, shotgun metagenomic sequencing was first used to characterize bacterial gut microbial communities and related functional changes in the AT group.ResultsWe found that the diversity and structure of the microbial community in the AT group were significantly changed compared to those in the BT group and the H group. The AT group showed a microbiota profile characterized by increased proportions of the phylum Firmicute, families Ruminococcaceae, and a reduction in the phylum Bacteroidota (elevated F/B ratio). To detect functional alteration, we discovered that secukinumab treatment may construct a more stable homeostasis of the gut microbiome with functional alteration. There were different KEGG pathways, such as the downregulated cardiovascular diseases pathway and the upregulated infectious diseases in the AT group. By metagenomic analysis, the metabolic functional pathway was changed after secukinumab therapy.DiscussionIt seems that gut microbiota investigation during biologic drug treatment is useful for predicting the efficacy and risks of drug treatment in disease.
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- 2023
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8. Activation of ryanodine-sensitive calcium store drives pseudo-allergic dermatitis via Mas-related G protein-coupled receptor X2 in mast cells
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Zhao Wang, Xi Zhao, Hongmei Zhou, Delu Che, Xiaojie Du, Dan Ye, Weihui Zeng, and Songmei Geng
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pseudo-allergy ,mast cell ,degranulation ,MRGPRX2 ,ryanodine receptor ,calcium ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Mast cell (MC) activation is implicated in the pathogenesis of multiple immunodysregulatory skin disorders. Activation of an IgE-independent pseudo-allergic route has been recently found to be mainly mediated via Mas-Related G protein-coupled receptor X2 (MRGPRX2). Ryanodine receptor (RYR) regulates intracellular calcium liberation. Calcium mobilization is critical in the regulation of MC functional programs. However, the role of RYR in MRGPRX2-mediated pseudo-allergic skin reaction has not been fully addressed. To study the role of RYR in vivo, we established a murine skin pseudo-allergic reaction model. RYR inhibitor attenuated MRGPRX2 ligand substance P (SP)-induced vascular permeability and neutrophil recruitment. Then, we confirmed the role of RYR in an MC line (LAD2 cells) and primary human skin-derived MCs. In LAD2 cells, RYR inhibitor pretreatment dampened MC degranulation (detected by β-hexosaminidase retlease), calcium mobilization, IL-13, TNF-α, CCL-1, CCL-2 mRNA, and protein expression activated by MRGPRX2 ligands, namely, compound 48/80 (c48/80) and SP. Moreover, the inhibition effect of c48/80 by RYR inhibitor was verified in skin MCs. After the confirmation of RYR2 and RYR3 expression, the isoforms were silenced by siRNA-mediated knockdown. MRGPRX2-induced LAD2 cell exocytosis and cytokine generation were substantially inhibited by RYR3 knockdown, while RYR2 had less contribution. Collectively, our finding suggests that RYR activation contributes to MRGPRX2-triggered pseudo-allergic dermatitis, and provides a potential approach for MRGPRX2-mediated disorders.
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- 2023
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9. STAG2 regulates interferon signaling in melanoma via enhancer loop reprogramming
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Zhaowei Chu, Lei Gu, Yeguang Hu, Xiaoyang Zhang, Man Li, Jiajia Chen, Da Teng, Man Huang, Che-Hung Shen, Li Cai, Toshimi Yoshida, Yifeng Qi, Zhixin Niu, Austin Feng, Songmei Geng, Dennie T. Frederick, Emma Specht, Adriano Piris, Ryan J. Sullivan, Keith T. Flaherty, Genevieve M. Boland, Katia Georgopoulos, David Liu, Yang Shi, and Bin Zheng
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Science - Abstract
STAG2 is a core subunit of the cohesin complex involved in DNA looping, but its transcriptional targets are largely unknown. Here the authors show STAG2 controls the 3D chromatin structure at the IRF9 locus to restrict IRF9 expression. Loss of STAG2 results in IRF9 activation, which in turn upregulates PD-L1 expression in cancer cells, suggesting a tumor suppressor function in immune evasion.
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- 2022
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10. Aloe‐emodin‐mediated antimicrobial photodynamic therapy against dermatophytosis caused by Trichophyton rubrum
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Wenpeng Ma, Miaomiao Zhang, Zixin Cui, Xiaopeng Wang, Xinwu Niu, Yanyan Zhu, Zhihong Yao, Feng Ye, Songmei Geng, and Chengcheng Liu
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Biotechnology ,TP248.13-248.65 - Abstract
Summary Trichophyton rubrum is responsible for the majority of dermatophytosis. Current systemic and topical antifungals against dermatophytosis are often tedious and sometimes unsatisfactory. Antimicrobial photodynamic therapy (aPDT) is a non‐invasive alternative suitable for the treatment of superficial fungal infections. This work investigated the photodynamic inactivation efficacy and effects of aloe‐emodin (AE), a natural photosensitizer (PS) against T. rubrum microconidia in vitro, and evaluated the treatment effects of AE‐mediated aPDT for T. rubrum‐caused tinea corporis in vivo and tinea unguium ex vivo. The photodynamic antimicrobial efficacy of AE on T. rubrum microconidia was evaluated by MTT assay. The inhibition effect of AE‐mediated aPDT on growth of T. rubrum was studied. Intracellular location of AE, damage induced by AE‐mediated aPDT on cellular structure and surface of microconidia and generation of intracellular ROS were investigated by microscopy and flow cytometry. The therapeutic effects of AE‐mediated aPDT against dermatophytosis were assessed in T. rubrum‐caused tinea corporis guinea pig model and tinea unguium ex vivo model. AE‐mediated aPDT effectively inactivated T. rubrum microconidia in a light energy dose‐dependent manner and exhibited strong inhibitory effect on growth of T. rubrum. Microscope images indicated that AE is mainly targeted to the organelles and caused damage to the cytoplasm of microconidia after irradiation through generation of abundant intracellular ROS. AE‐mediated aPDT demonstrated effective therapeutic effects for T. rubrum‐caused tinea corporis on guinea pig model and tinea unguium in ex vivo model. The results obtained suggest that AE is a potential PS for the photodynamic treatment of dermatophytosis caused by T. rubrum, but its permeability in skin and nails needs to be improved.
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- 2022
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11. Dysbiosis of gut microbiota and its correlation with dysregulation of cytokines in psoriasis patients
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Xinyue Zhang, Linjing Shi, Ting Sun, Kun Guo, and Songmei Geng
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Psoriasis ,Gut microbiome ,16S ,Microbiota ,Cytokines ,Microbiology ,QR1-502 - Abstract
Abstract Background Psoriasis is an inflammatory skin disease associated with multiple comorbidities and substantially diminishes patients’ quality of life. The gut microbiome has become a hot topic in psoriasis as it has been shown to affect both allergy and autoimmunity diseases in recent studies. Our objective was to identify differences in the fecal microbial composition of patients with psoriasis compared with healthy individuals to unravel the microbiota profiling in this autoimmune disease. Results We collected fecal samples from 30 psoriasis patients and 30 healthy controls, sequenced them by 16S rRNA high-throughput sequencing, and identified the gut microbial composition using bioinformatic analyses including Quantitative Insights into Microbial Ecology (QIIME) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Our results showed that different relative abundance of certain bacterial taxa between psoriasis patients and healthy individuals, including Faecalibacterium and Megamonas, were increased in patients with psoriasis. It’s also implicated that many cytokines act as main effect molecules in the pathology of psoriasis. We selected the inflammation-related indicators that were abnormal in psoriasis patients and found the microbiome variations were associated with the level of them, especially interleukin-2 receptor showed a positive relationship with Phascolarctobacterium and a negative relationship with the Dialister. The relative abundance of Phascolarctobacterium and Dialister can be regard as predictors of psoriasis activity. The correlation analysis based on microbiota and Inflammation-related indicators showed that microbiota dysbiosis might induce an abnormal immune response in psoriasis. Conclusions We concluded that the gut microbiome composition in psoriasis patients has been altered markedly and provides evidence to understand the relationship between gut microbiota and psoriasis. More mechanistic experiments are needed to determine whether the differences observed in gut microbiota are the cause or consequences of psoriasis and whether the relationship between gut microbiota and cytokines was involved.
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- 2021
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12. A Chinese case of Nakajo–Nishimura syndrome with novel compound heterozygous mutations of the PSMB8 gene
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Tao Jia, Yi Zheng, Cheng Feng, Tielin Yang, and Songmei Geng
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Nakajo-Nishimura syndrome ,PSMB8 ,Compound heterozygous mutations ,PRAAS ,Internal medicine ,RC31-1245 ,Genetics ,QH426-470 - Abstract
Abstract Background Nakajo-Nishimura syndrome (NNS) is an autosomal recessive heredity disorder, one of a spectrum of autoinflammatory diseases named proteasome-associated autoinflammatory syndrome (PRAAS) caused by mutations of PSMB8 gene. NNS is characterized by pernio-like skin rashes, intermittent fever, and long clubbed fingers and toes with joint contractures, partially with progressive lipomuscular atrophy, emaciation, hepatosplenomegaly and basal ganglion calcification. Case presentation We presented a sporadic case of NNS with compound heterozygous mutations in the PSMB8 gene. The 4-year-old boy was affected by progressive erythematous plaques on his nose and gradually involved hands and feet later with characteristic appearance of long clubbed fingers. The repetitive periodic intermittent fever was recorded. By gene sequencing, novel compound heterozygous mutations c.373C > T (p.R125C) and c.355G > A (p.D119N) in the PSMB8 gene were found. The patient responded well to low dosage of oral methylprednisolone. Conclusions We reported novel compound heterozygous mutations in PSMB8 in a sporadic Chinese NNS patient.
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- 2020
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13. Antifungal Effect of Antimicrobial Photodynamic Therapy Mediated by Haematoporphyrin Monomethyl Ether and Aloe Emodin on Malassezia furfur
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Zixin Cui, Miaomiao Zhang, Songmei Geng, Xinwu Niu, Xiaopeng Wang, Yanyan Zhu, Feng Ye, and Chengcheng Liu
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Malassezia furfur ,photodynamic antimicrobial effect ,haematoporphyrin monomethyl ether ,aloe emodin ,protease and lipase ,Microbiology ,QR1-502 - Abstract
Infectious dermatological diseases caused by Malassezia furfur are often chronic, recurrent, and recalcitrant. Current therapeutic options are usually tedious, repetitive, and associated with adverse effects. Alternatives that broaden the treatment options and reduce side effects for patients are needed. Antimicrobial photodynamic therapy (aPDT) is an emerging approach that is quite suitable for superficial infections. The aim of this study is to investigate the antimicrobial efficacy and effect of aPDT mediated by haematoporphyrin monomethyl ether (HMME) and aloe emodin (AE) on clinical isolates of M. furfur in vitro. The photodynamic antimicrobial efficacy of HMME and AE against M. furfur was assessed by colony forming unit (CFU) assay. The uptake of HMME and AE by M. furfur cells was investigated by fluorescence microscopy. Reactive oxygen species (ROS) probe and flow cytometry were employed to evaluate the intracellular ROS level. The effect of HMME and AE-mediated aPDT on secreted protease and lipase activity of M. furfur was also investigated. The results showed that HMME and AE in the presence of light effectively inactivated M. furfur cells in a photosensitizer (PS) concentration and light energy dose-dependent manner. AE exhibited higher antimicrobial efficacy against M. furfur than HMME under the same irradiation condition. HMME and AE-mediated aPDT disturbed the fungal cell envelop, significantly increased the intracellular ROS level, and effectively inhibited the activity of secreted protease and lipase of M. furfur cells. The results suggest that HMME and AE have potential to serve as PSs in the photodynamic treatment of dermatological diseases caused by M. furfur, but further ex vivo or in vivo experiments are needed to verify that they can meet the requirements for clinical practice.
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- 2021
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14. Expert consensus on the use of omalizumab in chronic urticaria in China
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Zuotao Zhao, MD, PhD, Tao Cai, MD, Hong Chen, MD, Liuqing Chen, MD, Yudi Chen, MD, Xiang Gao, MD, Xinghua Gao, MD, Songmei Geng, MD, Yinshi Guo, MD, Fei Hao, MD, Guodong Hao, MM, Yan Hu, MD, Hongzhong Jin, MD, Zhehu Jin, MD, Chengxin Li, MD, Haili Li, MD, Jie Li, MD, Yanming Li, MD, Yunsheng Liang, MD, Guanghui Liu, MD, Qiang Liu, MD, Hai Long, MD, Lin Ma, MD, Yuanyuan Shang, MM, Yuxin Song, MB, Zhiqiang Song, MD, Xiangyang Su, MD, Haijing Sui, MD, Qing Sun, MD, Yuemei Sun, MB, Jianping Tang, MD, Xunliang Tong, MD, Huiying Wang, MD, Gang Wang, MD, Lianglu Wang, MD, Siqin Wang, MB, Li Xiang, MD, Ting Xiao, MD, Zhiqiang Xie, MD., Leping Ye, MD, Yongmei Yu, MM, Chunlei Zhang, MD, PhD, Litao Zhang, MD, Shuchen Zhang, MD, PhD, Rui Zheng, MD, Lili Zhi, MM, Wei Zhou, MD, Ying Zou, MD, and Marcus Maurer, MD
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Chronic spontaneous urticaria ,Chronic inducible urticaria ,Treatment algorithm ,Omalizumab ,China ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Chronic urticaria (CU) is a debilitating skin disease that lasts for more than 6 weeks with wheals and/or angioedema, including chronic spontaneous urticaria (CSU) and chronic inducible urticaria (CIndU). In China, the prevalence of this disease is high, more than 1%, and on the rise. CU has a major impact on the quality of life (QoL) of patients who frequently experience sleep disturbance, depression, and anxiety. Nearly one-third of patients with CSU, in China, are resistant to second-generation H1-antihistamines (sgAHs), even at a fourfold dose (second line; off-label). Omalizumab is approved for the treatment of CSU treatment in Europe and shows remarkable efficacy and safety. In China, regulatory approval for the use of omalizumab is pending, and its use in clinical practice varies widely. Consensus on omalizumab CU treatment in China is urgently needed. The aim of this article is to propose a practical omalizumab treatment algorithm for the management of antihistamine-resistant CSU and CIndU in adults and special population including children and adolescents, and pregnant or breast feeding women, to guide daily clinical practice in China. In the development of this consensus, an expert group including mainly dermatologists, allergists, but also pulmonologists, ENTs, immunologists, and pediatricians in Allergic Disease Prevention and Control Committee, Chinese Preventive Medicine Association, reviewed the existing evidence and developed consensus on the use of omalizumab in CU patients from China. The goal of this consensus is to assist clinicians in making rational decisions in the management of refractory CU with omalizumab. The key clinical questions covered by the treatment algorithm are: 1) Omalizumab treatment routine strategy in both CSU and CIndU patients; 2) Recommended dose and treatment duration for different age stratification; 3) Treatment duration for CU patients with other allergic comorbidities; 4) Recommendation on omalizumab stopping strategy.
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- 2021
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15. Emerging Biomarkers and Therapeutic Strategies for Refractory Bullous Pemphigoid
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Tong Zhou, Bin Peng, and Songmei Geng
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bullous pemphigoid ,biomarkers ,severity ,refractory ,relapse ,prognosis ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Bullous pemphigoid (BP) is the most common autoimmune subepidermal blistering disorder in the elderly. Systemic and topical use of glucocorticoids and immunosuppressants has been shown to be effective in most patients. However, refractory BP patients are challenged to clinicians with severe clinical symptoms, resistance to treatment, and high relapse rate. How to predict and assess the refractory and severity of bullous pemphigoid is the key issue in clinical practice, and the urgent need for precision medicine in refractory patients is driving the search for biomarkers and biologics. Recently, some biomarkers, such as the level of specific autoantibodies and released cytokines, have been proposed as the potential parameters to reflect the disease severity and predict the treatment response and relapse of refractory BP. Moreover, new biologics targeting pathogenic antibodies, complement, Th2 axis, eosinophils, and Th17 axis have shown potent efficacy on refractory BP. Here, we review the literature and give an overview of emerging biomarkers and therapeutic strategies for refractory bullous pemphigoid to improve the prognosis of the patient.
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- 2021
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16. Rare presentation of Rothmund-Thomson syndrome with novel compound heterozygous mutations of the RECQL4 gene
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Xinyue Zhang, Songmei Geng, and Yi Zheng
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Dermatology ,RL1-803 - Published
- 2020
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17. Generalized type 2 segmental disseminated superficial actinic porokeratosis coexisted with multiple cutaneous squamous cell carcinomas: Analysis of two cases
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Qiang Zhao, Biao Yu, Hongmei Zhou, Cheng Feng, Xinyue Zhang, Yi Zheng, and Songmei Geng
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amyloidosis ,cornoid lamella ,cutaneous squamous cell carcinoma ,dermal amyloid deposit ,linear porokeratosis ,Pathology ,RB1-214 ,Microbiology ,QR1-502 - Abstract
Porokeratosis (PK) is defined as hyperpigmented macules or patches with a distinctive, ridge-like hyperkeratotic border which is histologically characterized by a cornoid lamella. Here, we report two cases of linear porokeratosis which converted to multiple cutaneous squamous cell carcinoma after long history progression. In addition, patient 2 was accompanied by secondary dermal amyloid deposits, which was rare reported.
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- 2020
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18. Aloe-Emodin-Mediated Photodynamic Therapy Induces Apoptosis in Basal Cell Carcinoma Cells via Activation of ERK/JNK Signaling Pathway
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Woodvine Otieno Odhiambo, Songmei Geng, Xiaopeng Wang, Xiaodong Chen, Mengting Qin, Meng Yuan, Yang Wang, Farooq Riaz, Chengcheng Liu, and Yanhong Ji
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Renewable energy sources ,TJ807-830 - Abstract
Basal cell carcinoma (BCC) is the most prevalent epidermal cancerous neoplasm. Previous studies have reported the noninvasive, cost-effective, and localized photodynamic therapy (PDT) approach to BCC treatment. This study investigated the photodynamic effects of aloe-emodin (AE), a natural anthraquinone photosensitizer (PS), on proliferation and apoptosis of BCC TE 354.T cell line. To evaluate the effects of AE-mediated PDT, we used various concentrations of AE (0, 2.5, 5, and 10 μM) and white light energy (0, 12, 24, and 36 J/cm2). CCK-8 assay was used to analyse cell viability following AE-mediated PDT. The cell death rate and reactive oxygen species (ROS) were assessed by flow cytometry. Western blotting was used to determine the effects of AE-mediated PDT on the apoptotic proteins, Akt, and MAPK pathways. AE-mediated PDT inhibited tumorigenic cell proliferation, consequently enhancing apoptosis in AE and PDT concentration and dose-dependent manner, respectively. Significantly increased TE 354.T cell apoptosis and intracellular ROS production were both observed after AE-mediated PDT. Following the AE-mediated PDT, cytochrome and antitumor p53 were elevated; however expression of Bcl-2 was significantly decreased. Significant caspase 3 elevation post-AE-mediated PDT suggested intrinsically driven apoptosis. Additionally, AE-mediated PDT significantly suppressed NF-κB, Akt, and ERK pathways while JNK expression was significantly increased. AE-mediated PDT induced TE 354.T cell apoptosis through the intracellular generation of ROS. Akt, ERK, and JNK all play various roles in ensuring successful TE 354.T tumor cell apoptosis.
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- 2021
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19. Multiple warty dyskeratoma on the scalp
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Qiang Zhao, Hongmei Zhou, and Songmei Geng
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Dermatology ,RL1-803 - Published
- 2019
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20. Dense Papules on the Face: A Quiz
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Hongmei Zhou, Qiang Zhao, Lihong Zhao, Qian Shang, Jing Li, and Songmei Geng
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Dermatology ,RL1-803 - Abstract
Abstract is missing (Quiz)
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- 2019
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21. Hyper IgE syndrome
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Yun Dang, JianWen Ren, YuanYuan Guo, and Songmei Geng
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Hyper IgE syndrome ,epilepsy ,eczema ,Dermatology ,RL1-803 - Abstract
Hyper IgE syndrome (HIES) is a rare disorder characterized by eczema, recurrent infections of the skin and lungs, typically with Staphylococcus aureus, Candida albicans and certain viruses, and elevated levels of serum IgE. Other clinical manifestations include characteristic facies (prominent forehead, broad nasal bridge and facial asymmetry), chronic eczematous dermatitis, retained primary dentition, recurrent pathological fractures, hyper-extensibility and scoliosis. The central nervous system (CNS) involvement in HIES has been rarely reported. Here we presented a case of HIES with rare associations of epilepsy in a young patient to raise awareness for this disorder.
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- 2014
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22. A retrospective study on the characteristics of treating nevus of ota by 1064-nm q-switched neodymium-doped yttrium aluminum garnet laser
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Yanting Liu, Weihui Zeng, and Songmei Geng
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Efficacy ,nevus of Ota ,Q-switched neodymium-doped yttrium aluminum garnet laser ,Dermatology ,RL1-803 - Abstract
Background: The Q-switched neodymium-doped yttrium aluminum garnet (QS Nd:YAG) laser has a significant effect in treating nevus of Ota, but there is lack of a retrospective study about the characteristics of efficacy. Aims and Objectives: To retrospectively analyze the correlation between the clinical characteristics and efficacy, complications, recurrence of QS Nd:YAG laser in treating nevus of Ota. Materials and Methods: One hundred and seventy-one Chinese patients (144 female, 27 male) of nevus of Ota were treated with the 1064-nm QS Nd:YAG laser. All cases were treated with fluencies of 4–8 J/cm2 and a spot size of 2–4 mm. Clinical photographs were taken before every treatment and patients were followed up by their clinicians. Results: One hundred and forty-five patients (84.8%) acquired more than 75% improvement with an average of 4.6 sessions. The treatment effect has no significant correlation with sex (P > 0.05). The blue-black and brown lesions improved more than the light-brown (P < 0.05). Hyperpigmentation affected two (1.2%) of the patients and hypopigmentation affected one patient (0.6%). No other adverse effect was observed. Recurrence was seen in two patients (1.2%). Conclusion: The 1064-nm QS Nd:YAG laser is effective with rare complications and recurrence in the treatment of nevus of Ota. The efficacy correlated with lesion color, which is meaningful to estimate the prognosis.
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- 2016
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23. A Multi-model Ensemble Method Using CNN and Maximum Correntropy Criterion for Basal Cell Carcinoma and Seborrheic Keratoses Classification.
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Leida Guo, Shaoyi Du, Yuting Chi, Wenting Cui, Panpan Song, Jihua Zhu, Songmei Geng, and Meifeng Xu
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- 2019
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24. Clinical guideline for the diagnosis and treatment of cutaneous warts (2022)
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Peiyao Zhu, Rui‐Qun Qi, Yang Yang, Wei Huo, Yuqing Zhang, Li He, Gang Wang, Jinhua Xu, Furen Zhang, Rongya Yang, Ping Tu, Lin Ma, Quanzhong Liu, Yuzhen Li, Heng Gu, Bo Cheng, Xiang Chen, Aijun Chen, Shengxiang Xiao, Hongzhong Jin, Junling Zhang, Shanshan Li, Zhirong Yao, Weihua Pan, Huilan Yang, Zhu Shen, Hao Cheng, Ping Song, Lingyu Fu, Hongxiang Chen, Songmei Geng, Kang Zeng, Jianjian Wang, Juan Tao, Yaolong Chen, Xiuli Wang, and Xing‐Hua Gao
- Subjects
Pregnancy ,Health Policy ,Humans ,Female ,General Medicine ,Warts ,Child ,Papillomaviridae - Abstract
Cutaneous warts caused by human papillomavirus are benign proliferative lesions that occur at any ages in human lives. Updated, comprehensive and systematic evidence-based guidelines to guide clinical practice are urgently needed.We collaborated with multidisciplinary experts to formulate this guideline based on evidences of already published literature, focusing on 13 clinical questions elected by a panel of experts. We adopted Grading of Recommendations Assessment, Development and Evaluation (GRADE) system to form classification of recommendations as well as the improved Delphi method to retain respective recommendations with a consensus degree of over 80%.Our guideline covered aspects of the diagnosis and treatment of cutaneous warts such as diagnostic gold standard, transmission routes, laboratory tests, treatment principle, clinical cure criterion, definitions, and treatments of common warts, flat warts, plantar warts, condyloma acuminatum, and epidermodysplasia verruciformis. Recommendations about special population such as children and pregnant women are also listed. In total, 49 recommendations have been obtained.It is a comprehensive and systematic evidence-based guideline and we hope this guideline could systematically and effectively guide the clinical practice of cutaneous warts and improve the overall levels of medical services.
- Published
- 2022
25. A case of pemphigoid gestationis successfully treated with dupilumab
- Author
-
Yanting Liu, Jingyi Yuan, Yumin Xia, Xueshan Du, and Songmei Geng
- Subjects
Infectious Diseases ,Dermatology - Published
- 2023
26. A rare severe tuberculosis cutis orificialis in a patient with compound heterozygous mutations in the <scp>PRF1</scp> gene
- Author
-
Longfei Zhu, Yang Dang, Mengyao Yi, Cheng Feng, and Songmei Geng
- Subjects
Infectious Diseases ,Dermatology - Published
- 2023
27. Diversity in the clinical presentation of generalized pustular psoriasis ( <scp>GPP</scp> ): A series of case vignettes from around the world
- Author
-
Siew Eng Choon, Boni E. Elewski, Hideki Fujita, Songmei Geng, Peter van de Kerkhof, Sicily Mburu, Lluís Puig, Ricardo Romiti, and Marina Venturini
- Subjects
Dermatology ,Molecular Biology ,Biochemistry - Published
- 2023
28. Chinese nontwin sisters suffer from poikiloderma with neutropenia harboring novel compound heterozygous USB1 gene mutations
- Author
-
Cong Yan, Tong Zhou, and Songmei Geng
- Subjects
Dermatology ,General Medicine - Published
- 2023
29. Suprabasin-derived polypeptides: SBSN(50-63) induces inflammatory response via TLR4-mediated mast cell activation
- Author
-
Tong Zhou, Xueshan Du, Lei Zhang, Yi Zheng, Tao Jia, Xiangjin Song, Delu Che, and Songmei Geng
- Subjects
Pharmacology ,Immunology ,Pharmacology (medical) - Published
- 2023
30. Luteolin inhibits <scp>FcεRΙ</scp> ‐ and <scp>MRGPRX2</scp> ‐mediated mast cell activation by regulating calcium signaling pathways
- Author
-
Yong Hao, Delu Che, Yanqin Yu, Lixia Liu, Shuhong Mi, Yang Zhang, Jinqi Hao, Wei Li, Musi Ji, Songmei Geng, and Jihai Shi
- Subjects
Mice, Inbred C57BL ,Pharmacology ,Mice ,Animals ,Calcium ,Calcium Signaling ,Mast Cells ,Luteolin ,Anaphylaxis ,Cell Degranulation ,Cell Line ,Receptors, G-Protein-Coupled - Abstract
Luteolin is a flavonoid found in many fruits, vegetables, and herbs. The antiinflammatory effects of luteolin have been reported. In this study, the effect of luteolin on allergic diseases and the underlying molecular mechanism were investigated. We found that luteolin inhibits Fc epsilon RΙ (FcεRΙ)- and Mas-related G protein-coupled receptor X2 (MRGPRX2)-mediated mast cells (MCs) activation, including degranulation and release of cytokines in vitro. Moreover, luteolin reduces the degree of swelling and Evans blue exudation of mice paw in a dose-dependent manner. The concentrations of histamine, TNF-α, MCP-1, IL-8, and IL-13 in mice serum are also decreased by luteolin administration. Our study reveals that luteolin can inhibit FcεRΙ- and MRGPRX2-mediated allergic responses in vivo and in vitro, and our results discover luteolin inhibited mast cells mediated anaphylactic reaction by inhibiting the phosphorylation level of PLCγ.
- Published
- 2022
31. Dehydroandrographolide targets <scp>CD300f</scp> and negatively regulated <scp>MRGPRX2</scp> ‐induced pseudo‐allergic reaction
- Author
-
Delu Che, Yi Zheng, Yajing Hou, Ting Li, Xueshan Du, and Songmei Geng
- Subjects
Mice, Inbred C57BL ,Pharmacology ,Disease Models, Animal ,Mice ,Hypersensitivity ,Animals ,Mast Cells ,Diterpenes ,Cell Degranulation ,Receptors, G-Protein-Coupled - Abstract
Mas-related G protein-coupled receptor X2 (MRGPRX2) mediates mast cells (MCs) activation, which is a key target for the treatment of allergic diseases. However, there are few drugs targeting MRGPRX2. Leukocyte mono-immunoglobulin-like receptor 3 (CD300f) is a negative regulator of FcεRΙ-mediated MC activation. However, the regulatory effect of CD300f on MRGPRX2 remains unclear. Dehydroandrographolide (DA) is a main contributor of Andrographis paniculata (Burm.f.) Nees (family: Acanthaceae) have been shown to inhibit type I hypersensitivity. The aim of this study was to determine whether DA negatively regulated MRGPRX2-mediated MC activation via CD300f and showed therapeutic effect on pseudo-allergic reactions. Mouse allergic models and MC degranulation were detected in vivo and in vitro, and inflammatory mediators were detected. siRNA interference and Biacore were used to verify the target. DA inhibited pseudo-allergic reactions by reducing vasodilation and serum cytokine levels in mice and inhibited MRGPRX2-mediated MC activation. The regulatory effect of DA was significantly decreased after the knockdown of CD300f expression. Moreover, DA upregulated the phosphorylation level of Src homology region 2 domain-containing phosphatase (SHP)-1 and SHP-2, which are key kinases in the negative regulatory signaling pathways associated with CD300f. In conclusion, DA negatively regulates MRGPRX2-mediated MC activation via CD300f to inhibit pseudo-allergic reactions.
- Published
- 2022
32. Efficient gene transfection of suspension cells by highly branched poly(β-amino ester)
- Author
-
Delu Che, Chenfei Wang, Zhili Li, Kaixuan Wang, Shuaiwei Sun, Xinyue Zhang, Yi Li, Zhengju Chen, Lei Guo, Yajing Hou, Dezhong Zhou, and Songmei Geng
- Subjects
General Chemistry - Published
- 2023
33. Hydroquinone Induced Skin Irritant Reaction Could Be Achieved by Activating Mast Cells via Mas-related G protein-coupled receptor X2
- Author
-
Yi Zheng, Xueshan Du, Lei Zhang, Tao Jia, Huan Zhang, Bin Peng, Yong Hao, Zhou Tong, Delu Che, and Songmei Geng
- Subjects
Dermatology ,Molecular Biology ,Biochemistry - Abstract
Hydroquinone (HQ), is one of the most effective drugs to treat hyperpigmentary disorders, but often causes skin irritation in clinic. Mast cell plays an important role in contact dermatitis and triggering pseudo-allergic reactions via MRGPRX2. Whether HQ induced skin irritant reaction through activating mast cells via MRGPRX2 remains unknown.To investigate the role of mast cells in HQ induced skin irritant reaction and verify whether MRGPRX2 participated in the HQ effect on mast cells which contributed to the pathogenesis of skin irritant reaction.A mouse model of HQ induced skin irritation was established to observe the local and systemic inflammation associated with mast cell receptor MrgprB2. Human mast cell LAD2 were used to verify the effect of HQ on mast cells via MRGPRX2 by knocking down with siRNA.Mast cells were involved in the development of HQ-induced irritant reaction and local inflammation are closely related to mast cell receptor MrgprB2. HQ could activate mast cells via MRGPRX2, causing changes in calcium concentration, degranulation, and release of inflammatory cytokines which lead to skin irritant reaction.HQ induced skin irritant reaction could be skin pseudo-allergic reactions achieved by activating mast cells via MRGPRX2.
- Published
- 2022
34. Wearable Hydrogel-Based Epidermal Sensor with Thermal Compatibility and Long Term Stability for Smart Colorimetric Multi-Signals Monitoring
- Author
-
Fei Han, Xueyong Xie, Tiansong Wang, Chaoyu Cao, Juju Li, Tianying Sun, Hao Liu, Songmei Geng, Zhao Wei, Jing Li, and Feng Xu
- Subjects
Biomaterials ,Biomedical Engineering ,Pharmaceutical Science - Abstract
Hydrogel-based wearable epidermal sensors (HWESs) have attracted widespread attention in health monitoring, especially considering their colorimetric readout capability. However, it remains challenging for HWESs to work at extreme temperatures with long term stability due to the existence of water. Herein, a wearable transparent epidermal sensor with thermal compatibility and long term stability for smart colorimetric multi-signals monitoring is developed, based on an anti-freezing and anti-drying hydrogel with high transparency (over 90% transmittance), high stretchability (up to 1500%) and desirable adhesiveness to various kinds of substrates. The hydrogel consists of polyacrylic acid, polyacrylamide, and tannic acid-coated cellulose nanocrystals in glycerin/water binary solvents. When glycerin readily forms strong hydrogen bonds with water, the hydrogel exhibits outstanding thermal compatibility. Furthermore, the hydrogel maintains excellent adhesion, stretchability, and transparency after long term storage (45 days) or at subzero temperatures (-20 °C). For smart colorimetric multi-signals monitoring, the freestanding smart colorimetric HWESs are utilized for simultaneously monitoring the pH, T and light, where colorimetric signals can be read and stored by artificial intelligence strategies in a real time manner. In summary, the developed wearable transparent epidermal sensor holds great potential for monitoring multi-signals with visible readouts in long term health monitoring.
- Published
- 2022
35. Successful treatment of psoriasis with secukinumab constructed a new homeostasis of gut microbiome
- Author
-
Xueshan Du, Cong Yan, Shuzhen Kong, Delu Che, Bin Peng, Longfei Zhu, Songmei Geng, and Gun Guo
- Abstract
Background: Secukinumab (an interleukin (IL)-17 inhibitor) has been reported to be able to alter gut microbiome composition in psoriatic patients. However, it still remains unclear the gut microbiota alteration and related functional changes caused by successful secukinumab therapy in psoriatic patients. In this study, fecal samples from healthy people as comtrol (H; n=35), psoriatic patients without therapy (BT; n=32) and patients after 5-month successful secukinumab treatment (AT; n=11) were collected., Then gut microbiomes were investigated using next-generation sequencing targeting 16S ribosomal RNA. Results: Successful secukinumab therapy caused a significantly elevated microbiota richness and biodiversity, as well as an alteration in the gut microbiota composition than psoriasis without therapy and healthy control. The psoriatic patients after secukinumab successful therapy showed a microbiota profile characterized by increased proportions of the phylum Firmicute, families Comamonadaceae and Erwiniaceae, and a reduction in the phylum Bacteroidota, compared to the other two groups. Besides, we discovered that secukinumab treatment group exhibited more significant abundance in contains mobile elements phenotype, form biofilms phenotype, gram positive phenotype, and less abundance in gram negative phenotype and potentially pathogenic phenotype by BugBase. Functional analysis showed the different COG pathways and KEGG pathways such as downregulated cardiovascular diseases pathway and upregulated infectious diseases between secukinumab therapy group and the other two groups.Conclusions: Secukinumab therapy enhanced the diversity of gut microbiota and altered the composition of the gut microbiome, which actually may construct a more stable homeostasis of gut microbiome with less potentially pathogenicity.
- Published
- 2022
36. Aloe‐emodin‐mediated antimicrobial photodynamic therapy against dermatophytosis caused by Trichophyton rubrum
- Author
-
Wenpeng Ma, Zixin Cui, Miaomiao Zhang, Feng Ye, Songmei Geng, Zhihong Yao, Xinwu Niu, Chengcheng Liu, Xiaopeng Wang, and Yanyan Zhu
- Subjects
Emodin ,medicine.medical_treatment ,Guinea Pigs ,Bioengineering ,Photodynamic therapy ,Trichophyton rubrum ,Applied Microbiology and Biotechnology ,Biochemistry ,Aloe emodin ,Microbiology ,03 medical and health sciences ,Anti-Infective Agents ,Tinea ,Trichophyton ,In vivo ,Onychomycosis ,medicine ,Animals ,Photosensitizer ,Aloe ,skin and connective tissue diseases ,030304 developmental biology ,0303 health sciences ,Photosensitizing Agents ,biology ,030306 microbiology ,Chemistry ,Arthrodermataceae ,bacterial infections and mycoses ,medicine.disease ,biology.organism_classification ,Antimicrobial ,Anti-Bacterial Agents ,Photochemotherapy ,bacteria ,Tinea capitis ,Reactive Oxygen Species ,Ex vivo ,Biotechnology ,medicine.drug - Abstract
Trichophyton rubrum is responsible for the majority of dermatophytosis. Current systemic and topical antifungals against dermatophytosis are often tedious and sometimes unsatisfactory. Antimicrobial photodynamic therapy (aPDT) is a non-invasive alternative suitable for the treatment of superficial fungal infections. This work investigated the photodynamic inactivation efficacy and effects of aloe-emodin (AE), a natural photosensitizer (PS) against T. rubrum microconidia in vitro, and evaluated the treatment effects of AE-mediated aPDT for T. rubrum-caused tinea corporis in vivo and tinea unguium ex vivo. The photodynamic antimicrobial efficacy of AE on T. rubrum microconidia was evaluated by MTT assay. The inhibition effect of AE-mediated aPDT on growth of T. rubrum was studied. Intracellular location of AE, damage induced by AE-mediated aPDT on cellular structure and surface of microconidia and generation of intracellular ROS were investigated by microscopy and flow cytometry. The therapeutic effects of AE-mediated aPDT against dermatophytosis were assessed in T. rubrum-caused tinea corporis guinea pig model and tinea unguium ex vivo model. AE-mediated aPDT effectively inactivated T. rubrum microconidia in a light energy dose-dependent manner and exhibited strong inhibitory effect on growth of T. rubrum. Microscope images indicated that AE is mainly targeted to the organelles and caused damage to the cytoplasm of microconidia after irradiation through generation of abundant intracellular ROS. AE-mediated aPDT demonstrated effective therapeutic effects for T. rubrum-caused tinea corporis on guinea pig model and tinea unguium in ex vivo model. The results obtained suggest that AE is a potential PS for the photodynamic treatment of dermatophytosis caused by T. rubrum, but its permeability in skin and nails needs to be improved.
- Published
- 2021
37. Gut Microbiome Alterations and Functional Prediction in Chronic Spontaneous Urticaria Patients
- Author
-
Songmei Geng, Xinyue Zhang, Linjing Shi, Jun Zhang, Kun Guo, and Zhaowei Chu
- Subjects
Adult ,Male ,0106 biological sciences ,Firmicutes ,Gut flora ,digestive system ,01 natural sciences ,Applied Microbiology and Biotechnology ,Feces ,RNA, Ribosomal, 16S ,010608 biotechnology ,Megasphaera ,medicine ,Humans ,Chronic Urticaria ,Bacteria ,biology ,Verrucomicrobia ,Bacteroidetes ,General Medicine ,biology.organism_classification ,medicine.disease ,Gastrointestinal Microbiome ,Immunology ,Dysbiosis ,Female ,Proteobacteria ,Biotechnology - Abstract
The effects of the gut microbiome on both allergy and autoimmunity in dermatological diseases have been indicated in several recent studies. Chronic spontaneous urticaria (CSU) is a disease involving allergy and autoimmunity, and there is no report detailing the role of microbiota alterations in its development. This study was performed to identify the fecal microbial composition of CSU patients and investigate the different compositions and potential genetic functions on the fecal microbiota between CSU patients and normal controls. The gut microbiota of CSU patients and healthy individuals were obtained by 16s rRNA massive sequencing. Gut microbiota diversity and composition were compared, and bioinformatics analysis of the differences was performed. The gut microbiota composition results showed that Firmicutes, Bacteroidetes, Proteobacteria, and Verrucomicrobia were dominant microbiota in CSU patients. The differential analysis showed that relative abundance of the Proteobacteria (p = 0.03), Bacilli (p = 0.04), Enterobacterales (p = 0.03), Enterobacteriaceae (p = 0.03) was significantly increased in CSU patients. In contrast, the relative abundance of Megamonas, Megasphaera, and Dialister (all p < 0.05) in these patients significantly decreased compared with healthy controls. The different microbiological compositions impacted normal gastrointestinal functions based on function prediction, resulting in abnormal pathways, including transport and metabolism. We found CSU patients exhibited gut microbiota dysbiosis compared with healthy controls. Our results indicated CSU is associated with gut microbiota dysbiosis and pointed out that the bacterial taxa increased in CSU patients, which might be involved in the pathogenesis of CSU. These results provided clues for future microbial-based therapies on CSU.
- Published
- 2021
38. Lipopolysaccharide reduces melanin synthesis in vitiligo melanocytes by regulating autophagy
- Author
-
Lijun Sun, Jingying Sun, Xueping Huo, Qing Feng, Yan Li, Xin Xie, and Songmei Geng
- Subjects
Lipopolysaccharides ,Melanins ,Toll-Like Receptor 4 ,Autophagy ,Vitiligo ,Humans ,Melanocytes ,Chloroquine ,Dermatology ,Ligands ,Molecular Biology ,Biochemistry - Abstract
Vitiligo is an autoimmune-related disease with a complex aetiology that involves innate immunity. Toll-like receptors (TLRs) are important parts of innate immunity and are related to a variety of autoimmune diseases, including vitiligo, through an unknown mechanism. In this study, we found that the TLR4 gene expression was increased in blood samples of patients with advanced stage vitiligo, and then, we evaluated the effect of TLR4 ligand lipopolysaccharide (LPS) on melanin synthesis in a vitiligo melanocyte cell line PIG3V and along with its mechanism. LPS suppressed melanin synthesis, downregulated the expression of melanin synthesis-related proteins and activated autophagy in vitiligo melanocytes. Inhibiting autophagy with 3-methyladenine or chloroquine blocked these effects. This suggests that LPS inhibits skin pigmentation by modulating autophagy, thus providing novel insights into the pathogenesis of vitiligo.
- Published
- 2022
39. Dual effect of tacrolimus on mast cell-mediated allergy and inflammation through MAS-related G protein-coupled receptor-X2
- Author
-
Xueshan Du, Delu Che, Yi Zheng, Yong Hao, Tao Jia, Xinyue Zhang, Bin Peng, and Songmei Geng
- Abstract
Background: Topical tacrolimus has been widely used in the treatment of inflammatory and immune dermatoses for its immunosuppression effect. However, a transient irritation like itching, burning induced by tacrolimus is common when initial application, which is similar to pseudo-allergic reaction. MAS-related G protein-coupled receptor-X2 (MRGPRX2) in mast cells (MCs) mediates drug-induced pseudo-allergic reaction and IgE-independent pruritis in chronic skin diseases. Whether MRGPRX2 participates in above tacrolimus adverse reaction should be addressed. Further, immunosuppression mechanism of tacrolimus on MCs is greatly ignored. Methods: Wild-type (WT) mice, kit mice and MrgprB2 deficient (MUT) mice were applied to explore the mechanism of initial irritant reaction and immunosuppression of tacrolimus on the skin MrgprB2 in MCs in vivo. LAD2 cells and MRGPRX2-knockdown LAD2 cells were used to confirm the regulation of MRGPRX2 by tacrolimus in vitro. Results: Tacrolimus could trigger IgE-independent dermatitis when initial application through MrgprB2-related MCs degranulation. Using FK-DNS, a fluorescently labeled tacrolimus, we found tacrolimus could bind to MRGPRX2 directly. Interestingly, after long-term tacrolimus treatment, the initial itching and inflammatory reaction faded away without IgE change. Hence, longstanding treatment with tacrolimus suppressed MRGPRX2/B2 expression and decreased inflammatory cytokines release. Conclusion: Our study provides for the first time a novel target for tacrolimus, demonstrating that short-term tacrolimus treatment induces pseudo-allergic reaction via MRGPRX2/B2 in MCs, while long-term treatment dampens MRGPRX2/B2 expression, leading to decreased inflammatory cytokines release and immune cells recruitment, which may contribute to its potent immunosuppression effect in the treatment of inflammatory and immune skin diseases.
- Published
- 2022
40. Clarithromycin-treated chronic spontaneous urticaria with the negative regulation of FcεRΙ and MRGPRX2 activation via CD300f
- Author
-
Delu Che, Tao Zhang, Tianxiao Zhang, Yi Zheng, Yajing Hou, Songmei Geng, and Langchong He
- Subjects
Pharmacology ,Receptors, Neuropeptide ,Mice ,Clarithromycin ,Immunology ,Immunology and Allergy ,Animals ,Chronic Urticaria ,Nerve Tissue Proteins ,Mast Cells ,Anaphylaxis ,Cell Degranulation ,Receptors, G-Protein-Coupled - Abstract
Mast cells (MCs) are main effector cells in chronic spontaneous urticaria (CSU). Both Fc epsilon RI (FcεRΙ)- and MAS-related G coupled receptor-X2 (MRGPRX2)-mediated MC activations affect CSU course. Leukocyte mono-immunoglobulin-like receptor 3 (CD300f) has been shown to regulate FcεRΙ activation. However, no study has verified CD300f is a target to cure CSU. Therefore this study aimed to verify whether clarithromycin (CLA) regulates FcεRΙ- and MRGPRX2-mediated MC activations via CD300f and shows therapeutic effect on CSU. The target of CLA was verification. CLA inhibited FcεRΙ- and MRGPRX2-mediated MC activations were shown in vivo and in vitro. A single-center, self-comparison study was performed, and CLA-treated CSU was investigated in 28 patients who were not sensitive to the third-generation antihistamines. Serum inflammatory mediators in patients before and after CLA administration were analyzed. CLA effectively inhibited type Ι anaphylactic reactions and pseudo-allergic reactions in mice. Moreover, CLA inhibited FcεRΙ- and MRGPRX2-mediated MC signaling pathway activation. Regulatory effects of CLA were decreased significantly after CD300f knockdown. CLA effectively alleviated the symptoms of wheal and itch and reduced serum cytokine levels in patients. CLA negatively regulated FcεRΙ- and MRGPRX2-mediated MC activation via CD300f and showed significant therapeutic effect on CSU.
- Published
- 2022
41. Glucagonoma syndrome with atypical necrolytic migratory erythema
- Author
-
Shujuan He, Songmei Geng, Jinjing Jia, and Weihui Zeng
- Subjects
medicine.medical_specialty ,Glucagonoma Syndrome ,Palliative treatment ,business.industry ,Histology ,Dermatology ,Necrolytic migratory erythema ,Histopathological examination ,medicine.disease ,Neuroendocrine tumour ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Infectious Diseases ,Eosinophilic infiltration ,030220 oncology & carcinogenesis ,Rare case ,medicine ,business - Abstract
Necrolytic migratory erythema is most commonly associated with glucagonoma syndrome. We report a rare case of glucagonoma syndrome with necrolytic migratory erythema presenting as pruritic papules and follicular pustules in a 57-year-old woman; showing eosinophilic infiltration on histology. However, the final diagnosis was confirmed by demonstrating neuroendocrine tumour on histopathological examination of the liver metastases. Nutrition therapy was administered as a palliative treatment. This case also highlights the atypical clinical features and nonspecific histology of necrolytic migratory erythema which makes the diagnosis difficult.
- Published
- 2021
42. Wearable Hydrogel‐Based Epidermal Sensor with Thermal Compatibility and Long Term Stability for Smart Colorimetric Multi‐Signals Monitoring (Adv. Healthcare Mater. 3/2023)
- Author
-
Fei Han, Xueyong Xie, Tiansong Wang, Chaoyu Cao, Juju Li, Tianying Sun, Hao Liu, Songmei Geng, Zhao Wei, Jing Li, and Feng Xu
- Subjects
Biomaterials ,Biomedical Engineering ,Pharmaceutical Science - Published
- 2023
43. UHRF1 Knockdown Attenuates Cell Growth, Migration, and Invasion in Cutaneous Squamous Cell Carcinoma
- Author
-
Zhaowei Chu, Songmei Geng, and Qingyan Li
- Subjects
0301 basic medicine ,Cancer Research ,Skin Neoplasms ,Ubiquitin-Protein Ligases ,Mice, Nude ,Biology ,Matrix metalloproteinase ,Pathogenesis ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Ubiquitin ,Cell Movement ,Cell Line, Tumor ,Ring finger ,medicine ,Animals ,Humans ,Neoplasm Invasiveness ,Protein kinase B ,Cell Proliferation ,Gene knockdown ,Cell growth ,General Medicine ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Apoptosis ,030220 oncology & carcinogenesis ,CCAAT-Enhancer-Binding Proteins ,Carcinoma, Squamous Cell ,biology.protein ,Cancer research ,Female ,Signal Transduction - Abstract
Ubiquitin like with PHD and ring finger domains 1 (UHRF1) contributes to the progression of many cancers. Here, we firstly observed UHRF1 was elevated in cutaneous squamous cell carcinoma (cSCC) and related to the differentiation stages. Knockdown of UHRF1 in A431 and Scl-1 attenuated cell proliferation, migration, and invasion, leading to G2/M cell cycle arrest and apoptosis. Through a mouse xenograft model, we found UHRF1 deficiency ameliorated tumor growth. These results may be associated with destruction of multiple signal pathways. In summary, our results suggest UHRF1 is involved in the pathogenesis of cSCC and may be a therapeutic target.
- Published
- 2020
44. Dermcidin-derived polypeptides: DCD(86-103) induced inflammatory reaction in the skin by activation of mast cells via ST2
- Author
-
Delu Che, Tao Jia, Xinyue Zhang, Lei Zhang, Xueshan Du, Yi Zheng, Tong Zhou, Xiangjing Song, and Songmei Geng
- Subjects
Molecular Docking Simulation ,Inflammation ,Mice ,Dermcidins ,Immunology ,Immunology and Allergy ,Animals ,Psoriasis ,Cytokines ,Mast Cells ,Peptides ,Interleukin-1 Receptor-Like 1 Protein - Abstract
Psoriasis is a chronic inflammatory disease. Mast cells are significantly increased and activated in the lesions of patients with psoriasis, contributing to psoriatic inflammation. Dermcidin (DCD) is a natural antibacterial peptide secreted by sweat glands and is usually transported to the epidermal surface by sweat. Whether DCD is involved in mast cell activation remains unclear and the mechanisms by which DCD is involved in skin inflammatory reactions require further investigation.We investigated whether dermcidin-derived polypeptides DCD(86-103) activate mast cells and induce skin inflammatory reactions that contribute to psoriasis. Wild-type mice were treated with DCD(86-103) to observe the inflammatory reactions in the skin and cytokine release in vivo. The release of inflammatory mediators by mouse primary mast cells and LAD2 cells was measured in vitro. Molecular docking analysis, molecular dynamics simulation, and siRNA transfection were used to identify DCD(86-103).DCD(86-103) caused a skin inflammatory reaction in wild-type mice via cytokine release. Moreover, DCD(86-103) directly activated mast cells and induced cytokine release in vitro. ST2 may be a key receptor that mediates the activation effect of DCD(86-103) on mast cells leading to cytokine release.DCD(86-103) may have induced an inflammatory reaction and participated in the occurrence and development of psoriasis.
- Published
- 2022
45. Biosafety and differentially expressed genes analysis of melanoma cells treated with cold atmospheric plasma
- Author
-
Lihong Zhao, Cong Yan, Shuzhen Kong, Tao Jia, Zhaowei Chu, Li Yang, Jian Wu, Songmei Geng, and Kun Guo
- Subjects
Plasma Gases ,Cell Line, Tumor ,General Engineering ,General Physics and Astronomy ,Humans ,RNA ,General Materials Science ,Apoptosis ,General Chemistry ,Containment of Biohazards ,Melanoma ,General Biochemistry, Genetics and Molecular Biology - Abstract
Cold atmospheric plasma (CAP) has attracted increasing attention due to its anti-bacterial and anti-tumor effects. Melanoma is an aggressive malignancy with increasing incidence rate and poor prognosis. Evaluating cell viability, apoptosis rate and reactive species injection efficiency of melanoma cells and human keratinocyte cells (HaCaT) treated with CAP to analyze biological safety of CAP. RNA-sequencing (RNA-seq) of A875 cells before and after treatment was performed to further explore the anti-tumor mechanism of CAP. CAP had a more significant biological effect on melanoma cells than HaCaT cells by inhibiting proliferation and promoting apoptosis. RNA-sequencing analysis showed that besides MAPK and p53 apoptotic signaling pathways, necroptosis and autophagy also played important roles in CAP-induced melanoma cells death. CAP can selectively kill melanoma cells and has good biosafety cytologically. Besides apoptosis, CAP can induce cell death via autophagy and necroptosis.
- Published
- 2022
46. Hydroxychloroquine-induced acute generalized exanthematous pustulosis in an orofacial granulomatosis patient with homozygous IL36RN mutation
- Author
-
Cong Yan, Qian Shang, and Songmei Geng
- Subjects
Acute Generalized Exanthematous Pustulosis ,Interleukins ,Homozygote ,Mutation ,Humans ,Granulomatosis, Orofacial ,Dermatology ,General Medicine ,Hydroxychloroquine - Published
- 2022
47. Cyclic poly(β-amino ester)s with enhanced gene transfection activity synthesized through intra-molecular cyclization
- Author
-
Chenfei Wang, Xiaobei Huang, Litao Sun, Qiuxia Li, Zhili Li, Haiyang Yong, Delu Che, Cong Yan, Songmei Geng, Wenxin Wang, and Dezhong Zhou
- Subjects
Cell Survival ,Polymers ,Metals and Alloys ,General Chemistry ,DNA ,Transfection ,Catalysis ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,HEK293 Cells ,Cyclization ,Materials Chemistry ,Ceramics and Composites ,Humans ,Luciferases - Abstract
Cyclic poly(β-amino ester)s (CPAEs) are synthesized via sequential Michael addition and free radical initiating ring-closure reaction, and show superior gene transfection compared to their linear counterparts.
- Published
- 2022
48. A 1-Year-Old Boy with Multiple Papules
- Author
-
Ya-xiang Li, Long-fei Zhu, and Songmei Geng
- Published
- 2022
49. A Young Boy with Hyperpigmented and Hypopigmented Macules
- Author
-
Jingang An and Songmei Geng
- Published
- 2022
50. An Infant with Disseminated Red Scaling Papules
- Author
-
Jiahui Hu, Lihong Zhao, and Songmei Geng
- Published
- 2022
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