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The CRISPR/Cas9 system is a powerful genome editing tool that has made enormous impacts on next-generation molecular diagnostics and therapeutics, especially for genetic disorders that traditional therapies cannot cure. Currently, CRISPR-based gene editing is widely applied in basic, preclinical, and clinical studies. In this review, we attempt to identify trends in clinical studies involving CRISPR techniques to gain insights into the improvement and contribution of CRISPR/Cas technologies compared to traditional modified modalities. The review of clinical trials is focused on the applications of the CRISPR/Cas systems in the treatment of cancer, hematological, endocrine, and immune system diseases, as well as in diagnostics. The scientific basis underlined is analyzed. In addition, the challenges of CRISPR application in disease therapies and recent advances that expand and improve CRISPR applications in precision medicine are discussed.

The proliferation of artificial intelligence (AI) has opened up new avenues for the modeling of power electronics with ultra-fast transient responses, such as wide-bandgap (WBG) devices. This article highlights the significance of ultra-fast transient device-level hardware emulation for the DC railway microgrid (DRM) in real-time. To this end, the proposed approach partitions the DRM power system by transmission line method (TLM) and employs gated recurrent unit (GRU) and electromagnetic transient (EMT) modeling techniques for system-level subsystems. Meanwhile, for WBG devices, gallium nitride (GaN) high electron mobility transistors (HEMT) and silicon carbide (SiC) insulated gate bipolar transistors (IGBT) are modeled using a novel physical feature neuron network (PFNN), which offers high flexibility with a variable time-step (as low as 1 ns), thereby improving the accuracy, efficiency and accelerating the emulation on the field-programmable gate array (FPGA). The effectiveness of the proposed approach is confirmed by comparing the emulation results with offline simulation results obtained from PSCAD/EMTDC for system-level and SaberRD for device-level transients. The proposed PFNN approach provides strong versatility, ultra-fast transient emulation capability, and significantly improved accuracy, which bodes well for the future of power electronics device-level emulation.

Abstract Hyperspectral imaging has broad applications and impacts in areas including environmental science, weather, and geo/space exploration. The intrinsic spectral–spatial structures and potential multi-level features in different frequency bands make multilayer graph an intuitive model for hyperspectral images (HSI). To study the underlying characteristics of HSI and to take the advantage of graph signal processing (GSP) tools, this work proposes a multilayer graph spectral analysis for hyperspectral images based on multilayer graph signal processing (M-GSP). More specifically, we present multilayer graph (MLG) models and tensor representations for HSI. By exploring multilayer graph spectral space, we develop MLG-based methods for HSI applications, including unsupervised segmentation and supervised classification. Our experimental results demonstrate the strength of M-GSP in HSI processing and spectral–spatial information extraction.

Human papillomavirus (HPV) is one of the most common sexually transmitted infections (STIs). However, despite widespread under-vaccination amongst men and the importance of vaccinating both sexes to curb the spread of HPV, research has focused on promoting HPV vaccination predominantly amongst women. Therefore, the current study examines the effectiveness of different informational interventions in promoting vaccination intentions amongst heterosexual men. In a preregistered study of 583 unvaccinated adult men, we randomly assigned participants to one of four informational interventions aimed at promoting awareness of HPV risks and vaccine uptake: (1) risks to oneself (n = 145), (2) risks to their female partner (n = 144), (3) risks to oneself and their female partner (n = 153), and (4) general vaccine information (n = 153). Amongst participants reporting a sexual history (67%), intentions to get vaccinated significantly increased by 10.75 points on a 100-point scale (p < 0.01) after they received information about the risks of HPV for both themselves and their female partner, compared to receiving information about only their own HPV risk. These findings provide valuable guidance for public health officials and policymakers into the effectiveness of different messaging strategies in promoting HPV vaccination amongst adult male populations to increase vaccination rates.