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29 results on '"Sorrentino, Alida"'

13. Proteomics for depicting the secreted protein patterns of Lactiplantibacillus plantarumstrains isolated from different food matrices

Author

Mazzeo, Maria Fiorella, Sorrentino, Alida, Morandi, Stefano, Abouloifa, Houssam, Asehraou, Abdeslam, Brasca, Milena, and Siciliano, Rosa Anna

Abstract

Lactiplantibacillus plantarum(formerly known as Lactobacillus plantarum), a bacterial species isolated from a wide variety of ecological niches, plays an important role in food industries, being used as a starter culture of food fermentations and contributing to improve flavor, texture, sensorial qualities, and shelf life. It also improves the nutritional and functional properties of fermented foods through the biosynthesis of bioactive compounds and several strains exhibit probiotic features. In recent years, this bacterial species has also shown great potential for controlling plant pathogenic fungi. Shotgun proteomic approach and label free quantitative analyses were used to realize a detailed catalog of the proteins secreted by five L. plantarumstrains isolated from vegetable foods or dairy products and exhibiting specific function features. In particular, these strains showed in vitroinhibitory activity against bacteria and molds. Results led to identify 602 proteins differently present in the secretome of the analyzed strains. For instance, proteins of application interest such as bacteriocins, glucansucrase, glycoside hydrolase and chitin binding proteins were differentially secreted in the five strains.

Published
2024
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14. Coupling immunomagnetic separation on magnetic beads with matrix-assisted laser desorption ionization-time of flight mass spectrometry for detection of Staphylococcal enterotoxin

Author

Schosser, Gitta, Kacer, Petr, Kuzma, Marek, Szilagyi, Zoltan, Sorrentino, Alida, Manzo, Carlo, Pizzano, Rosa, Malorni, Livia, and Pocsfalvi, Gabriella

Subjects
Enterotoxins -- Research, Mass spectrometry -- Technology application, Proteomics -- Research, Staphylococcus -- Physiological aspects, Technology application, Biological sciences
Abstract

A method is developed based on selective immunoaffinity capture enrichment that can be performed in a single-tube experiment for selective isolation of the protein toxin. The application of this method to complex biological matrices is shown by selective detection of Staphylococcal enterotoxin B (SEB) seen in different matrices like the cultivation media of Staphylococcus aureus and raw milk samples.

Published
2007

16. Pathogenic Role of Associated Adherent-Invasive Escherichia coli in Crohn's Disease

Author

Mazzarella, Giuseppe, Iaquinto, Gaetano, De Luca, Antonio, Perna, Angelica, Marano, Angela, Lucariello, Angela, Rotondi Aufiero, Vera, Sorrentino, Alida, Melina, Raffaele, Guerra, Germano, Taccone, Fabio, Mazzarella, Giuseppe, Iaquinto, Gaetano, De Luca, Antonio, Perna, Angelica, Marano, Angela, Lucariello, Angela, Rotondi Aufiero, Vera, Sorrentino, Alida, Melina, Raffaele, Guerra, Germano, and Taccone, Fabio

Abstract

Several lines of evidence suggest that adherent-invasive Escherichia coli (AIEC) strains play an important role in Crohn's disease (CD). The objective of this study was to investigate the pathogenic role of two AIEC strains, LF82 and O83:H1, in CD patients. Organ cultures of colonic biopsies from patients were set up to assess the effects of LF82 and O83:H1 on the expression of CEACAM6, LAMP1, HLA-DR, ICAM1 by immunohistochemistry and of IL-8, IFNʏ, and TNF-α genes by RT-PCR. Moreover, on Caco2 cells, we analyzed the cell cycle, the expression of MGMT and DNMT1 genes, and DNA damage induced by LF82 and O83:H1, by FACS, RT-PCR, and DAPI staining, respectively. Epithelial and lamina propria mononuclear cells (LPMNC) expression of CEACAM6 and LAMP1 were higher in biopsies cultured in the presence of both O83:H1 and LF82 than in biopsies cultured with non-pathogenic E. coli. Both AIEC strains induced increased expression of ICAM-1 on blood vessels and HLA-DR on LPMNC. We observed higher levels of TNF-α, IFN-γ, and IL-8 transcripts in biopsies cultured with both AIEC strains than in those cultured with NP. Both LF82 and O83:H1, block the cell cycle into S phase, inducing DNA damage, and modulate the expression of DNMT1 and MGMT genes. Our data suggest that LF82 and 083:H1 strains of E. coli are able to increase in CD colonic biopsies the expression of all the pro-inflammatory cytokines and all the mucosal immune markers investigated. J. Cell. Physiol. 232: 2860–2868, 2017. © 2016 Wiley Periodicals, Inc., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2017

19. Coupling Immunomagnetic Separation on Magnetic Beads with MALDI-TOF Mass Spectrometry for the Detection of Staphylococcal Enterotoxin B

Author

Schlosser Gitta, Kacer Petr, Kuzma Marek, Szilagyi Zoltan, Sorrentino Alida, Manzo Carla, Pizzano Rosa, Malorni Livia, and Pocsfalvi Gabriella Katalin

Subjects
staphylococcus aureus, MALDI-TOF, Staphylococcal enterotoxin B, hemic and immune systems, chemical and pharmacologic phenomena, affinity capture, immunomagnetic separation, biological factors
Abstract

The growing importance of mass spectrometry for the identification and characterization of bacterial protein toxins is a consequence of the improved sensitivity and specificity of mass spectrometry-based techniques, especially when these techniques are combined with affinity methods. Here we describe a novel method based on the use of immunoaffinity capture and matrix-assisted laser desorption ionization-time of flight mass spectrometry for selective purification and detection of staphylococcal enterotoxin B (SEB). SEB is a potent bacterial protein toxin responsible for food poisoning, as well as a potential biological warfare agent. Unambiguous detection of SEB at low-nanogram levels in complex matrices is thus an important objective. In this work, an affinity molecular probe was prepared by immobilizing anti-SEB antibody on the surface of para-toluene-sulfonyl-functionalized monodisperse magnetic particles and used to selectively isolate SEB. Immobilization and affinity capture procedures were optimized to maximize the density of anti-SEB immunoglobulin G and the amount of captured SEB, respectively, on the surface of magnetic beads. SEB could be detected directly "on beads" by placing the molecular probe on the matrix-assisted laser desorption ionization target plate or, alternatively, "off beads" after its acidic elution. Application of this method to complex biological matrices was demonstrated by selective detection of SEB present in different matrices, such as cultivation media of Staphylococcus aureus strains and raw milk samples.

Published
2007
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20. Chemical, Volatile Profile and Shelf Life of Muffin Enriched with Supplementation Chestnut Cream.

Author

Boscaino, Floriana, Cammarota, Giancarlo, Ottombrino, Antonio, Nazzaro, Melissa, Siano, Francesco, Volpe, Maria G., and Sorrentino, Alida

Subjects
SHELF-life dating of food, MUFFINS, CHESTNUT, VOLATILE organic compounds, LINOLEIC acid
Abstract

The supplementation of chestnut cream in a new muffin formulation was investigated to evaluate the use of chestnut derivatives in baked goods. To assess the muffins qualities, with and without potassium sorbate, were evaluated the physical-chemical properties, fatty acids and volatile organic compounds (VOCs) profiles and shelf life (30 days). The fatty acid composition showed that the highest concentrations were evidenced for linoleic acid (47.3%), oleic acid (30.4%) and palmitic acid (14.2%). The total microbial count (TMC), yeasts and molds loads increased during the storage but remained under limit imposed by law. The VOCs detected are produced during baking process as a result of thermally induced reactions while phenolic compounds could derive from the chestnut cream. The electronic nose results showed that the 'fingerprints' of VOCs are not overlapping, confirming the HS-SPME-GC/MS data. According to the results, the formulation of new muffin could expand the array of commodities and further enlarge, the market for chestnut derivatives. Practical Application The introduction of the new sweet chestnut cream muffin formulation could represent a chance to increase the use of chestnut derivatives and could be alternative ingredients in baked goods. In fact, the supplementation of chestnut cream may give interesting properties as better physico-chemical properties, fatty acids, volatile organic compounds and a good shelf life. Moreover, they preserve the nutritional characteristics of the starting fruit. [ABSTRACT FROM AUTHOR]

Published
2017
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27. Survey of lactic acid bacteria isolated during the advanced stages of the ripening of Parmigiano Reggiano cheese

Author

*, RAFFAELE COPPOLA, ‡, NANNI, MAURO, IORIZZO, MASSIMO, SORRENTINO, ALIDA, SORRENTINO, ELENA, and GRAZIA, LUIGI

Abstract

Parmigiano Reggiano is a hard, cooked cheese produced in specific areas of Northern Italy. The raw material is obtained by mixing the partly skimmed evening milk and whole morning milk. The mixture is then heated to 22°C, and natural whey starter is added at 28–30 g/l after 1–2 min, bringing the pH of the mixture to 6·2–6·3. After coagulation, which takes ~15 min and occurs at 32–33°C owing to the addition of calf rennet powder, the curd is broken up for 2–4 min, cut into fragments and cooked at a temperature raised gradually to 42–44°C and then more quickly to 55–56°C over 10–15 min. The curd is left undisturbed, covered by the whey, for 40–60 min, then removed and placed inside a circular wooden mould. The cheese is held at ~20°C for 3 d during which it is turned at frequent intervals to facilitate complete whey drainage. The cheese, which now has its typical shape and size, is then salted by immersion in brine (260–280 g NaCl/l at 16–17°C) for 20–24 d. During this period the cheese absorbs 15–18 g NaCl/kg and its weight decreases by 4%. During the subsequent period of ripening (12–24 months) in store rooms at 16–18°C and 85% moisture, the cheese is frequently turned. At the end of ripening the cheese is cylindrical in shape, with a slightly convex side, 0·22–0·24 m high, 0·40–0·45 m diam. weighs 35–36 kg, has 320–330 g fat/kg dry matter and a minutely granulated internal structure with small holes formed by the activity of some heterofermentative lactic acid bacteria.

Published
1997

28. Modulation of the cytokine profile in Caco-2 cells by faecal lactobacilli and bifidobacteria from individuals with distinct dietary habits

Author

Diomira Luongo, Annachiara Coppola, A. Sorrentino, Raffaella Di Cagno, Ilario Ferrocino, Luca Simone Cocolin, Erasmo Neviani, Paolo Bergamo, Lucia Treppiccione, Silvia Turroni, Mauro Rossi, Luongo, Diomira, Coppola, Annachiara, Treppiccione, Lucia, Bergamo, Paolo, Sorrentino, Alida, Ferrocino, Ilario, Turroni, Silvia, Neviani, Erasmo, Di Cagno, Raffaella, Cocolin, Luca, and Rossi, Mauro

Subjects
0301 basic medicine, Adult, Male, Thymic stromal lymphopoietin, Adolescent, Enterocyte, medicine.medical_treatment, Immunology, Biochemistry, Microbiology, 03 medical and health sciences, Food Preferences, Immune system, Thymic Stromal Lymphopoietin, Immunity, Lactobacillus, medicine, Immunology and Allergy, Humans, Molecular Biology, Diet Enterocyte Immunity Microbiota, biology, Microbiota, Interleukin-8, Hematology, Dendritic cell, Feeding Behavior, Middle Aged, biology.organism_classification, Diet, 030104 developmental biology, medicine.anatomical_structure, Cytokine, Caco-2, Cytokines, Tetradecanoylphorbol Acetate, Female, Bifidobacterium, Caco-2 Cells
Abstract

Enterocytes are actively involved in the defense against pathogens and they limit penetration of commensal microbes into tissues. They also have an important role in gut immunity as enterocytes confer mucosal dendritic cell specialisation. On the other hand, the microbiota is directly involved in the development and modulation of the intestinal immune system. Particularly, lactobacilli and bifidobacteria play a primary role in shaping the immune response. We further explored this issue by evaluating whether functional differences in Caco-2 cells could characterise faecal populations of lactobacilli (155 samples) and bifidobacteria (110 samples) isolated from three dietary cohorts (omnivores, ovo-lacto-vegetarians and vegans) recruited at four Italian centres (Turin, Parma, Bologna and Bari). According to our findings, tested bacteria were unable to modulate expression of IL-8, IL-10, TGF-β or thymic stromal lymphopoietin (TSLP) cytokines in unstimulated Caco-2 cells. Conversely, in phorbol 12-myristate 13-acetate and ionomycin (PMA/Io) stimulated Caco-2 cells, lactobacilli from the omnivorous group and all bifidobacteria significantly down-regulated IL-8. Notably, both genera also lowered the TSLP expression in stimulated Caco-2 cells, regardless of the diet regimen. By further examining these data on the basis of geographical origin, we found that lactobacilli from the vegetarian group recruited in Bari, significantly up-regulated this cytokine. In conclusion, we highlighted a peculiar immune-modulatory activity profile for lactobacilli on enterocytes undergoing a stimulatory signal, which was associated with a specific dietary habit. Furthermore, the geographical area had a significant impact on the inflammatory potential of members of the Lactobacillus genus.

Published
2017

29. Pathogenic Role of Associated Adherent-Invasive Escherichia coli in Crohn's Disease.

Author

Mazzarella G, Perna A, Marano A, Lucariello A, Rotondi Aufiero V, Sorrentino A, Melina R, Guerra G, Taccone FS, Iaquinto G, and De Luca A

Subjects
Adult, Aged, Caco-2 Cells, Colon immunology, Colon metabolism, Colon pathology, Crohn Disease immunology, Crohn Disease metabolism, Crohn Disease pathology, Cytokines immunology, Cytokines metabolism, Escherichia coli immunology, Escherichia coli isolation & purification, Escherichia coli Infections immunology, Escherichia coli Infections metabolism, Escherichia coli Infections pathology, Female, Host-Pathogen Interactions, Humans, Immunity, Mucosal, Inflammation Mediators immunology, Inflammation Mediators metabolism, Intestinal Mucosa immunology, Intestinal Mucosa metabolism, Intestinal Mucosa pathology, Male, Middle Aged, Tissue Culture Techniques, Virulence, Young Adult, Bacterial Adhesion, Colon microbiology, Crohn Disease microbiology, Escherichia coli pathogenicity, Escherichia coli Infections microbiology, Intestinal Mucosa microbiology
Abstract

Several lines of evidence suggest that adherent-invasive Escherichia coli (AIEC) strains play an important role in Crohn's disease (CD). The objective of this study was to investigate the pathogenic role of two AIEC strains, LF82 and O83:H1, in CD patients. Organ cultures of colonic biopsies from patients were set up to assess the effects of LF82 and O83:H1 on the expression of CEACAM6, LAMP1, HLA-DR, ICAM1 by immunohistochemistry and of IL-8, IFNʏ, and TNF-α genes by RT-PCR. Moreover, on Caco2 cells, we analyzed the cell cycle, the expression of MGMT and DNMT1 genes, and DNA damage induced by LF82 and O83:H1, by FACS, RT-PCR, and DAPI staining, respectively. Epithelial and lamina propria mononuclear cells (LPMNC) expression of CEACAM6 and LAMP1 were higher in biopsies cultured in the presence of both O83:H1 and LF82 than in biopsies cultured with non-pathogenic E. coli. Both AIEC strains induced increased expression of ICAM-1 on blood vessels and HLA-DR on LPMNC. We observed higher levels of TNF-α, IFN-γ, and IL-8 transcripts in biopsies cultured with both AIEC strains than in those cultured with NP. Both LF82 and O83:H1, block the cell cycle into S phase, inducing DNA damage, and modulate the expression of DNMT1 and MGMT genes. Our data suggest that LF82 and 083:H1 strains of E. coli are able to increase in CD colonic biopsies the expression of all the pro-inflammatory cytokines and all the mucosal immune markers investigated. J. Cell. Physiol. 232: 2860-2868, 2017. © 2016 Wiley Periodicals, Inc., (© 2016 Wiley Periodicals, Inc.)

Published
2017
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