1. Occurrence and molecular characterization of Giardia duodenalis in lambs in Djelfa, the central steppe of Algeria
- Author
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Salem Belkessa, Djamel Baroudi, Ahcene Hakem, Karim Tarik Adjou, Myriam Thomas, Yaoyu Feng, Soumaya Benhassine, Dawn M. Roellig, Xiaohua Chen, AbdElkarim Laatamna, Lihua Xiao, Laboratory of Exploration and Valorisation of Steppic Ecosystems, Université Ziane Achour de Djelfa, École nationale supérieure véterinaire d'Alger, European Centre for Disease Prevention and Control (ECDC), Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Mohamed Khider [Biskra], South China Agricultural University (SCAU), National Natural Science Foundation of China (31820103014), 111 Project (D20008), and École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé
- Subjects
Giardiasis ,medicine.medical_specialty ,Veterinary medicine ,Genotype ,Sequence analysis ,Epidemiology ,030231 tropical medicine ,Protozoan Proteins ,Cattle Diseases ,Sheep Diseases ,Biology ,03 medical and health sciences ,Feces ,Zoonosis ,0302 clinical medicine ,Medical microbiology ,MESH: Giardia lamblia ,Glutamate Dehydrogenase ,medicine ,Prevalence ,Animals ,Direct fluorescent antibody ,Phylogeny ,2. Zero hunger ,0303 health sciences ,Sheep ,General Veterinary ,MESH: Giardiasis ,030306 microbiology ,Glutamate dehydrogenase ,MESH: Sheep Diseases ,General Medicine ,Lambs ,medicine.disease ,Diarrhea ,Infectious Diseases ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Giardia duodenalis ,Insect Science ,Algeria ,Parasitology ,Cattle ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,medicine.symptom ,Giardia lamblia ,Triose-Phosphate Isomerase - Abstract
International audience; Little is known of the prevalence and genetic identity of Giardia duodenalis in sheep in Algeria. The present study aimed at characterizing G. duodenalis in lambs up to 6 months of age in Djelfa, Algeria. A total of 346 fecal specimens were collected from 28 farms and screened for G. duodenalis cysts by zinc sulfate flotation microscopy, and positive specimens were confirmed using a direct immunofluorescence assay. Microscopy-positive specimens were analyzed by PCR and sequence analysis of the triosephosphate isomerase and glutamate dehydrogenase genes to determine G. duodenalis assemblages. Coprological examination indicated that the overall infection rate was 7.0% (24/346). Lambs under 3 months of age had higher infection rate (18/197, 9.0%) than older (6/149, 4.0%) animals, and animals with diarrhea (7/44, 16.0%) had higher infection rate than animals without diarrhea (17/302, 5.6%). PCR sequence analyses of the 15 G. duodenalis isolates revealed the presence of assemblages A in 6 isolates, assemblage E in 7 isolates, and both in 2 isolates. Assemblage A was only found in pre-weaned lambs with diarrhea, while assemblage E was mostly found in post-weaned lambs without diarrhea. The assemblage E isolates from sheep were genetically related to those from cattle in Algeria, while assemblage A isolates were from a well-known subtype prevalent in humans. Data generated from the study improve our understanding of the transmission of G. duodenalis in Algeria.
- Published
- 2020
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