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15. Shifting KRAS hotspot mutations inhibition paradigm in colorectal cancer

Author

Bras, Ana Rita, primary, Lopes, Ana, additional, Mendes, Nuno, additional, Costa, Paulo J., additional, Ferreira, Anabela, additional, Granja, Sara, additional, Silva, Ana Paula, additional, Tortosa, Francisco, additional, Baltazar, Fatima, additional, Gartner, Fatima, additional, Sousa, Maria Joao, additional, Valente, Andreia, additional, and Preto, Ana, additional

Published
2023
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31. Ethanol tolerance of sugar transport, and the rectification of stuck wine fermentations

Author

Santos, Julia, Sousa, Maria Joao, Cardoso, Helena, Inacio, Joao, Silva, Sofia, Spencer-Martins, Isabel, and Lento, Cecilia

Subjects
Fermentation -- Research, Wine and wine making -- Microbiology, Wine and wine making -- Research, Biological sciences
Abstract

The incomplete consumption of sugar resulting from stuck wine fermentation is associated with important economic losses. One of the solutions to this serious problem consists of reinoculating the brew with a yeast starter culture that is both alcohol tolerant and a vigorous fructose fermenter. The present work aimed to select yeast strains capable of restarting stuck wine fermentations, and identify key parameters that contribute to the efficiency of the strains. Commercial and non-commercial Saccharomyces wine strains were tested, as well as strains of the fermentative non-Saccharomyces species Zygosaccharomyces bailii and Torulaspora delbrueckii. Although the latter species were shown to be more resistant to a combination of ethanol- and acetic-acid-induced cell death, commercial Saccharomyces cerevisiae strains were the most efficient fructose consumers in medium simulating a stuck fermentation. Stationary-phase S. cerevisiae cells performed better than inocula prepared from exponentially growing cultures, which correlates with the higher resistance to ethanol of non-growing populations. Stationary-phase cells pre-adapted to ethanol did not improve fructose consumption rates; this was in contrast to exponential-phase cells that benefited from prior incubation in ethanol-containing medium. Notably, a correlation was observed between yeast fructose consumption capacity and glucose (or fructose) transport. Our results challenge the current belief that ethanol tolerance, expressed in terms of cell viability, is a reliable criterion for the selection of yeast strains to restart stuck fermentations. Instead, this capacity seems to be based on sugar transport and its resistance to ethanol. In an attempt to further improve cell viability in the presence of high ethanol concentrations, hybrid strains of T. delbrueckfi and S. cerevisiae were produced, and they showed high potential as restarter strains. The present work opens perspectives for the application of innovative strategies in the wine-making industry.

Published
2008

32. The spoilage yeast zygosaccharomyces bailii forms mitotic spores: a screening method for haploidization

Author

Rodrigues, Fernando, Ludovico, Paula, Sousa, Maria Joao, Steensma, H. Yde, Corte-Real, Manuela, and Leao, Cecilia

Subjects
Yeast fungi -- Genetic aspects, Haploidy -- Analysis, Microbial mutation -- Methods, Spores (Botany) -- Analysis, Biological sciences
Abstract

In the usually diploid zygosaccharomyces bailii, treatment with benomyl and selection on canavanine media yields haploid strains from which recessive mutants can be isolated.

Published
2003

33. Saccharomyces cerevisiae commits to a programmed cell death process in response to acetic acid

Author

Ludovico, Paula, Sousa, Maria Joao, Silva, Manuel T., Leao, Cecilia, and Corte-Real Manuela

Subjects
Saccharomyces -- Physiological aspects, Cell death -- Physiological aspects, Oxidation, Physiological -- Research, Acetic acid -- Physiological aspects, Biological sciences
Abstract

Research demonstrates that an apoptotic phenotype in Saccharomyces cerevisiae is inducible via oxidative stress. Results show that cells exposed to 20-200 millimolar acetic acid for 200 minutes at pH 3.0 experience cell dealth.

Published
2001

35. Mechanism underlying the transport and intracellular metabolism of acetic acid in the presence of glucose in the yeast Zygosaccharomyces bailii

Author

Sousa, Maria Joao, Rodrigues, Fernando, Corte-Real, Manuela, and Leao, Cecilia

Subjects
Yeast -- Physiological aspects, Acetic acid -- Observations, Metabolism -- Observations, Biological sciences
Abstract

Zygosaccharomyces bailii is a food and beverage spoilage yeast species and the mechanism concerned with transport of acetic acid into the cell and its regulation is a peculiar trait of the species. The mechanisms underlying the utilization of acetic acid by Z. bailii ISA 1307 strain, in the presence of glucose is considered, and evidence indicates that the regulation of membrane transport of acetic acid and acetyl-CoA synthetase activity contributes to Z. bailii's high resistance to environments containing sugars and acetic acid mixtures.

Published
1998

37. Hexose transport in Torulaspora delbrueckii: identification of Igt1, a new dual-affinity transporter

Author

European Commission, Fundação para a Ciência e a Tecnologia (Portugal), Pacheco, Andreia, Donzella, Lorena, Hernández-López, M. José, Almeida, Maria Judite, Prieto, Jose Antonio, Rández Gil, Francisca, Morrisey, John P., Sousa, Maria Joao, European Commission, Fundação para a Ciência e a Tecnologia (Portugal), Pacheco, Andreia, Donzella, Lorena, Hernández-López, M. José, Almeida, Maria Judite, Prieto, Jose Antonio, Rández Gil, Francisca, Morrisey, John P., and Sousa, Maria Joao

Abstract

Torulaspora delbrueckii is a yeast species receiving increasing attention from the biotechnology industry, with particular relevance in the wine, beer and baking sectors. However, little is known about its sugar transporters and sugar transport capacity, frequently a rate-limiting step of sugar metabolism and efficient fermentation. Actually, only one glucose transporter, Lgt1, has been characterized so far. Here we report the identification and characterization of a second glucose transporter gene, IGT1, located in a cluster, upstream of LGT1 and downstream of two other putative hexose transporters. Functional characterization of IGT1 in a Saccharomyces cerevisiae hxt-null strain revealed that it encodes a transporter able to mediate uptake of glucose, fructose and mannose and established that its affinity, as measured by Km, could be modulated by glucose concentration in the medium. In fact, IGT1-transformed S. cerevisiae hxt-null cells, grown in 0.1% glucose displayed biphasic glucose uptake kinetics with an intermediate- (Km = 6.5 ± 2.0 mM) and a high-affinity (Km = 0.10 ± 0.01 mM) component, whereas cells grown in 2% glucose displayed monophasic kinetics with an intermediate-affinity (Km of 11.5 ± 1.5 mM). This work contributes to a better characterization of glucose transport in T. delbrueckii, with relevant implications for its exploitation in the food industry.

Published
2020

38. Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and their implications on the resistance of the yeast to acidic environments

Author

Sousa, Maria Joao, Miranda, Leticia, Corte-Real, Manuela, and Leao, Cecilia

Subjects
Acetic acid -- Observations, Biological transport -- Research, Yeast fungi -- Biotechnology, Alcohol -- Physiological aspects, Biological sciences
Abstract

Ethanol suppresses the transport and accumulation of acetic acid in cells of Zygosaccharomyces bailii. This protects the cells from the possible negative effects of acetic acid. Ethanol controls the acid concentration inside the cell and keeps it below toxic levels. The acid enters the cell through a transporter protein by diffusion. The mechanism of ethanol-induced transport suppression enables Z. bailii to tolerate environments containing fermentation end products.

Published
1996

40. Catheter ablation of atrial fibrillation in patients with heart failure with reduced ejection fraction: Real world experience from six European centers

Author

Providência, Rui, primary, Asmundis, Carlo, additional, Chun, Julian, additional, Chierchia, Gianbattista, additional, Defaye, Pascal, additional, Anselme, Frédéric, additional, Creta, Antonio, additional, Lambiase, Pier D., additional, Schmidt, Boris, additional, Chen, Shaojie, additional, Hunter, Ross J., additional, Combes, Stephane, additional, Honarbakhsh, Shohreh, additional, Combes, Nicolas, additional, Sousa, Maria Joao, additional, Jebberi, Zeynab, additional, Albenque, Jean‐Paul, additional, and Boveda, Serge, additional

Published
2019
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45. Guidelines and recommendations on yeast cell death nomenclature

Author

Carmona-Gutierrez, Didac, Bauer, Maria Anna, Zimmermann, Andreas, Aguilera, Andres, Austriaco, Nicanor, Ayscough, Kathryn, Balzan, Rena, Bar-Nun, Shoshana, Barrientos, Antonio, Belenky, Peter, Blondel, Marc, Braun, Ralf J., Breitenbach, Michael, Burhans, William C., Büttner, Sabrina, Cavalieri, Duccio, Chang, Michael, Cooper, Katrina F., Corte-Real, Manuela, Costa, Vitor, Cullin, Christophe, Dawes, Ian, Dengjel, Jorn, Dickman, Martin B., Eisenberg, Tobias, Fahrenkrog, Birthe, Fasel, Nicolas, Frohlich, Kai-Uwe, Gargouri, Ali, Giannattasio, Sergio, Goffrini, Paola, Gourlay, Campbell W., Grant, Chris M., Greenwood, Michael T., Guaragnella, Nicoletta, Heger, Thomas, Heinisch, Juergen, Herker, Eva, Herrmann, Johannes M., Hofer, Sebastian, Jimenez-Ruiz, Antonio, Jungwirth, Helmut, Kainz, Katharina, Kontoyiannis, Dimitrios P., Ludovico, Paula, Manon, Stephen, Martegani, Enzo, Mazzoni, Cristina, Megeney, Lynn A., Meisinger, Chris, Nielsen, Jens, Nystrom, Thomas, Osiewacz, Heinz D., Outeiro, Tiago F., Park, Hay-Oak, Pendl, Tobias, Petranovic, Dina, Picot, Stephane, Polcic, Peter, Powers, Ted, Ramsdale, Mark, Rinnerthaler, Mark, Rockenfeller, Patrick, Ruckenstuhl, Christoph, Schaffrath, Raffael, Segovia, Maria, Severin, Fedor F., Sharon, Amir, Sigrist, Stephan J., Sommer-Ruck, Cornelia, Sousa, Maria Joao, Thevelein, Johan M., Thevissen, Karin, Titorenko, Vladimir, Toledano, Michel B., Tuite, Mick, Voegtle, F. -Nora, Westermann, Benedikt, Winderickx, Joris, Wissing, Silke, Woelfl, Stefan, Zhang, Zhaojie J., Zhao, Richard Y., Zhou, Bing, Galluzzi, Lorenzo, Kroemer, Guido, Madeo, Frank, Carmona-Gutierrez, Didac, Bauer, Maria Anna, Zimmermann, Andreas, Aguilera, Andres, Austriaco, Nicanor, Ayscough, Kathryn, Balzan, Rena, Bar-Nun, Shoshana, Barrientos, Antonio, Belenky, Peter, Blondel, Marc, Braun, Ralf J., Breitenbach, Michael, Burhans, William C., Büttner, Sabrina, Cavalieri, Duccio, Chang, Michael, Cooper, Katrina F., Corte-Real, Manuela, Costa, Vitor, Cullin, Christophe, Dawes, Ian, Dengjel, Jorn, Dickman, Martin B., Eisenberg, Tobias, Fahrenkrog, Birthe, Fasel, Nicolas, Frohlich, Kai-Uwe, Gargouri, Ali, Giannattasio, Sergio, Goffrini, Paola, Gourlay, Campbell W., Grant, Chris M., Greenwood, Michael T., Guaragnella, Nicoletta, Heger, Thomas, Heinisch, Juergen, Herker, Eva, Herrmann, Johannes M., Hofer, Sebastian, Jimenez-Ruiz, Antonio, Jungwirth, Helmut, Kainz, Katharina, Kontoyiannis, Dimitrios P., Ludovico, Paula, Manon, Stephen, Martegani, Enzo, Mazzoni, Cristina, Megeney, Lynn A., Meisinger, Chris, Nielsen, Jens, Nystrom, Thomas, Osiewacz, Heinz D., Outeiro, Tiago F., Park, Hay-Oak, Pendl, Tobias, Petranovic, Dina, Picot, Stephane, Polcic, Peter, Powers, Ted, Ramsdale, Mark, Rinnerthaler, Mark, Rockenfeller, Patrick, Ruckenstuhl, Christoph, Schaffrath, Raffael, Segovia, Maria, Severin, Fedor F., Sharon, Amir, Sigrist, Stephan J., Sommer-Ruck, Cornelia, Sousa, Maria Joao, Thevelein, Johan M., Thevissen, Karin, Titorenko, Vladimir, Toledano, Michel B., Tuite, Mick, Voegtle, F. -Nora, Westermann, Benedikt, Winderickx, Joris, Wissing, Silke, Woelfl, Stefan, Zhang, Zhaojie J., Zhao, Richard Y., Zhou, Bing, Galluzzi, Lorenzo, Kroemer, Guido, and Madeo, Frank

Abstract

Elucidating the biology of yeast in its full complexity has major implications for science, medicine and industry. One of the most critical processes determining yeast life and physiology is cellular demise. However, the investigation of yeast cell death is a relatively young field, and a widely accepted set of concepts and terms is still missing. Here, we propose unified criteria for the definition of accidental, regulated, and programmed forms of cell death in yeast based on a series of morphological and biochemical criteria. Specifically, we provide consensus guidelines on the differential definition of terms including apoptosis, regulated necrosis, and autophagic cell death, as we refer to additional cell death routines that are relevant for the biology of (at least some species of) yeast. As this area of investigation advances rapidly, changes and extensions to this set of recommendations will be implemented in the years to come. Nonetheless, we strongly encourage the authors, reviewers and editors of scientific articles to adopt these collective standards in order to establish an accurate framework for yeast cell death research and, ultimately, to accelerate the progress of this vibrant field of research.

Published
2018
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46. Impact of Body Mass Index on the Outcomes of Catheter Ablation of Atrial Fibrillation: A European Observational Multicenter Study.

Author

Providência, Rui, Adragão, Pedro, de Asmundis, Carlo, Chun, Julian, Chierchia, Gianbattista, Defaye, Pascal, Anselme, Fréderic, Creta, Antonio, Lambiase, Pier D., Schmidt, Boris, Chen, Shaojie, Cavaco, Diogo, Hunter, Ross J., Carmo, João, Combes, Stephane, Honarbakhsh, Shohreh, Combes, Nicolas, Sousa, Maria Joao, Jebberi, Zeynab, and Albenque, Jean-Paul

Published
2019
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