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7 results on '"Souza, Ariana B."'

5. Antimicrobial Evaluation of Diterpenes from Copaifera langsdorffii Oleoresin Against Periodontal Anaerobic Bacteria

Author

Souza, Ariana B., primary, Souza, Maria G. M. de, additional, Moreira, Maísa A., additional, Moreira, Monique R., additional, Furtado, Niege A. J. C., additional, Martins, Carlos H. G., additional, Bastos, Jairo K., additional, Santos, Raquel A. dos, additional, Heleno, Vladimir C. G., additional, Ambrosio, Sergio Ricardo, additional, and Veneziani, Rodrigo C. S., additional

Published
2011
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6. RP-HPLC analysis of manool-rich Salvia officinalisextract and its antimicrobial activity against bacteria associated with dental caries

Author

Moreira, Monique R., Souza, Ariana B., Moreira, Maísa A., Bianchi, Thamires C., Carneiro, Luiza J., Estrela, Fernanda T., dos Santos, Raquel A., Januário, Ana H., Martins, Carlos H.G., Ambrosio, Sérgio R., and Veneziani, Rodrigo C.S.

Abstract

In this paper we screened the dichloromethane extract from the aerial parts of Salvia officinalisL., Lamiaceae, against a representative panel of microorganisms that cause caries, conducted a bioassay-guided fractionation to establish themselves the most active metabolite (manool) and determined the Salvia officinalisfraction with the manool highest concentration to be used to activate an ingredient in oral care products such as toothpastes and mouthwashes. Both manool and S. officinalisextract showed very promising minimal inhibitory concentration values (between 6.24 and 31.36μg.ml−1) and time kill curves against the primary causative agents of dental caries (Streptococcus mutans) revealed that, at twice its minimal bactericidal concentration (12.48μg.ml−1), manool required 6h to completely kill the bacteria. Salvia officinalisextract at twice its minimal bactericidal concentration (31.36μg.ml−1) needed 12h. The results achieved with Salvia officinalisextract motivated us to develop and validate an analytical RP-HPLC method to detect and determine manool in this extract. The validation parameters were satisfactorily met and evaluated allows us to consider the developed method suitable for use in different labs. In conclusion, our results evidenced that the manool-rich S. officinalisextract can be considered an analytically validated alternative to develop novel and effective antimicrobial agents against the main bacteria responsible for dental caries.

Published
2013
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7. In vitro Evaluation of Copaifera oblongifolia Oleoresin Against Bacteria Causing Oral Infections and Assessment of Its Cytotoxic Potential.

Author

da S Moraes T, Leandro LF, de O Silva L, Santiago MB, Souza AB, Furtado RA, Tavares DC, Veneziani RC, Ambrósio SR, Bastos JK, and Martins CH

Subjects
Chlorhexidine administration & dosage, Chlorhexidine pharmacology, Dental Caries microbiology, Dental Caries prevention & control, Humans, Microbial Sensitivity Tests, Mouth Diseases microbiology, Mouth Diseases prevention & control, Mouthwashes administration & dosage, Mouthwashes pharmacology, Anti-Bacterial Agents pharmacology, Bacteria drug effects, Biofilms drug effects, Fabaceae physiology, Plant Extracts chemistry
Abstract

The oral cavity, which harbors more than 750 bacterial species, is one of the most diverse sites of the human body. Some of these bacteria have been associated with oral diseases, such as dental caries and endodontic infections. We report on the antimicrobial and cytotoxic activities of Copaifera oblongifolia oleoresin against bacteria that cause caries and endodontic infections. The aim of this study is to determine the minimum (MIC) and the bactericidal (MBC) inhibitory concentrations as well as the biofilm inhibition ability (through determination of MBIC50) of the C. oblongifolia oleoresin. This study also investigated the bactericidal kinetics (time-kill curves) and the synergistic effect of the C. oblongifolia oleoresin. Additionally, this study evaluated the cytotoxic activity of the oleoresin toward V79 cells by means of the colony-forming assay. The C. oblongifolia oleoresin gave promising MIC and MBC values, which ranged from 25 to 200 μg/mL. Analysis of the MBIC50values of the oleoresin revealed it displayed biofilm inhibitory activity against all the assayed bacteria. Analysis of the bactericidal kinetics showed different behaviors of the oleoresin against the tested bacteria at the different time intervals and concentrations assayed in this study. An additive effect of the oleoresin with chlorhexidine dihydrochloride occurred only for S. mitis and A. actinomycetemcomitans. The C. oblongifolia oleoresin showed cytotoxic activity at concentrations ≥ 625 μg/mL.

Published
2016
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