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Miscanthus (giganteus) is a relatively new energy crop. Its mechanical properties are important for the design and modification of harvesting and processing machines or equipment. The cutting strength is critical to improve the field performance of mowing and other cutting mechanisms. The effects of the cutting blade type, sample supporting method, and sample locations (upper or lower) where it was taken from a single plant stem on the cutting force and energy were studied. A serrated cutting blade and a flat blade were selected to cut Miscanthus samples at the node and internode using a high-speed impact tester. The cutting force, sample diameter, and cutting speed were recorded. The specific cutting force and energy were then calculated based on the cross-sectional area of the stem sample. The average diameter of the Miscanthus samples used for this study was 9.5 mm. The blade cutting speeds for all treatments were ranged from 8.2 ms−1 to 11.3 ms−1. Overall, the maximum specific cutting force and energy of the flat blade were found at the upper portion of a plant stem, which was 441 N cm−2 and 8.3 J cm−2 at the node when one side of the stem sample was fixed, and 469 N cm−2 and 12.1 J cm−2 at the internode if both sides of the sample were fixed. The cutting strengths at the node and internode were significantly different no matter at the upper portion or lower portion of the plant. When using the serrated blade, the maximum specific cutting force was also found at the upper node section with a value of 511 N cm−2 and 437 N cm−2 for one-side fixed and two-side fixed, respectively. Meanwhile, the corresponding maximum specific cutting energies for these two cases were 10.5 J cm−2 and 10.3 J cm−2, respectively. Statistical analysis showed that the blade types and stem sample locations significantly affected the specific cutting force and energy with a 95% confidence level. The sample support methods did not make significant differences when comparing the specific cutting force and energy; but the actual cutting force and energy were significantly different. [ABSTRACT FROM AUTHOR]

Miscanthus (giganteus) is a relatively new energy crop. Its mechanical properties are important for the design and modification of harvesting and processing machines or equipment. The cutting strength is critical to improve the field performance of mowing and other cutting mechanisms. The effects of the cutting blade type, sample supporting method, and sample locations (upper or lower) where it was taken from a single plant stem on the cutting force and energy were studied. A serrated cutting blade and a flat blade were selected to cut Miscanthus samples at the node and internode using a high-speed impact tester. The cutting force, sample diameter, and cutting speed were recorded. The specific cutting force and energy were then calculated based on the cross-sectional area of the stem sample. The average diameter of the Miscanthus samples used for this study was 9.5 mm. The blade cutting speeds for all treatments were ranged from 8.2 ms−1 to 11.3 ms−1. Overall, the maximum specific cutting force and energy of the flat blade were found at the upper portion of a plant stem, which was 441 N cm−2 and 8.3 J cm−2 at the node when one side of the stem sample was fixed, and 469 N cm−2 and 12.1 J cm−2 at the internode if both sides of the sample were fixed. The cutting strengths at the node and internode were significantly different no matter at the upper portion or lower portion of the plant. When using the serrated blade, the maximum specific cutting force was also found at the upper node section with a value of 511 N cm−2 and 437 N cm−2 for one-side fixed and two-side fixed, respectively. Meanwhile, the corresponding maximum specific cutting energies for these two cases were 10.5 J cm−2 and 10.3 J cm−2, respectively. Statistical analysis showed that the blade types and stem sample locations significantly affected the specific cutting force and energy with a 95% confidence level. The sample support methods did not make significant differences when comparing the specific cutting force and energy; but the actual cutting force and energy were significantly different.

The diaphragm muscle is essential for breathing, and its dysfunctions can be fatal. Many disorders affect the diaphragm, including muscular dystrophies. Despite the clinical relevance of targeting the diaphragm, there have been few studies evaluating diaphragm function following a given experimental treatment, with most of these involving anti-inflammatory drugs or gene therapy. Cell-based therapeutic approaches have shown success promoting muscle regeneration in several mouse models of muscular dystrophy, but these have focused mainly on limb muscles. Here we show that transplantation of as few as 5000 satellite cells directly into the diaphragm results in consistent and robust myofiber engraftment in dystrophin- and fukutin-related protein-mutant dystrophic mice. Transplanted cells also seed the stem cell reservoir, as shown by the presence of donor-derived satellite cells. Force measurements showed enhanced diaphragm strength in engrafted muscles. These findings demonstrate the feasibility of cell transplantation to target the diseased diaphragm and improve its contractility. [ABSTRACT FROM AUTHOR]

Background. Long jump is an athletic event that requires strength and speed abilities, both alone and in combination, and the way they condition each other has effects on athletic performance. The explosive force is decisive in this event and the speed on the run up, as well as obtaining the optimal speed near the takeoff board, creates optimal conditions to perform a successful jump. Aims. The aim of this study was to identify whether and to what extent correlations exist between force speed parameters and competition performance in under 16-18 years old women long jumpers. Methods. The research took place in two stages under similar conditions for all sub jects; the first stage at the Romanian National Indoor Athletic Championship 2023 and the second stage at the Romanian National Outdoor Women’s Athletic Championship 2023. 15 subjects were chosen for this study all long jumpers participating in the National Athletic Championships of Romania aged 16-18 years (x=13.87, SD=0.75). The measurements took place at both competitions under the same conditions and with the same measuring devices and the following parameters were investigated: speed on the last 10 m of momentum, 1 maximal repetition, verti.cal jump and afferent flight time, performance obtained in the competition. Results. The study identified significant correlations in both indoor and outdoor testing between test performance and speed over the last 10 m of the run up, r = -0.547, n = 15, p = 0.035 and r = -0.547, n = 15, p = 0.035, between vertical jump and flight time, r = 1, n = 15, p ˂ 0.001. The correlations between the performance obtained in competition and the other variables are statistically insignificant for both tests, for indoor testing: r = 0.151, p = 0.591 for flight time of the vertical jump, r = 0.209, p = 0.455 for one maximal repetition, r = 0.165, p = 0.556 for vertical jump and for outdoor testing: r = 0.151, p = 0.591 for the flight time of the vertical jump, r = 0.209, p = 0.455 for 1 maximal repetition, r = 0.165, p = 0.556 for vertical jump. Conclusions. Based on the results recorded in this study, strong negative linear correlations were determined between the speed on the last 10 m of the run-up and the performance obtained in the event, reinforcing the claim that a high speed on the run-up can lead to better performance in the long jump event. The lack of correlations between force parameters and long jump performance suggests that physical training at the level of the researched group is precarious and confirms the need to intervene on how to develop its specific strength. [ABSTRACT FROM AUTHOR]

Abstract—Relations are proposed for calculating combined conditions for drawing with heating during metal forming. The workpiece material is taken to be anisotropic and viscoplastic. The extremum upper-boundary plasticity theorem and the damage kinetics equations of a deformed material are used. Calculated data are obtained. [ABSTRACT FROM AUTHOR]

Synergist ablation (SA) is an experimental procedure for the induction of hypertrophy. However, SA causes a decrease in specific force (i.e., force per cross-sectional area), likely due to excessive muscle use. Here, we investigated the mechanisms behind the SA-induced intrinsic contractile dysfunction, especially focusing on the excitation-contraction (EC) coupling. Male Wistar rats had unilateral surgical ablation of gastrocnemius and soleus muscles to induce compensatory hypertrophy in the plantaris muscles. Two weeks after SA, plantaris muscle was dissected from each animal and used for later analyses. SA significantly increased the mean fiber cross-sectional area (+18%). On the other hand, the ratio of depolarization-induced force to the maximum Ca2+-activated specific force, an indicator of sarcoplasmic reticulum (SR) Ca2+ release, was markedly reduced in mechanically skinned fibers from the SA group (-51%). These functional defects were accompanied by an extensive fragmentation of the SR Ca2+ release channel, the ryanodine receptor 1 (RyR1), and a decrease in the amount of other triad proteins (i.e., DHPR, STAC3, and junctophilin1). SA treatment also caused activation of calpain-1 and increased the amount of NADPH oxidase 2, endoplasmic reticulum (ER) stress proteins (i.e., Grp78, Grp94, PDI, and Ero1), and lipid peroxidation [i.e., 4-hydroxynonenal (4-HNE)] in SA-treated muscles. Our findings show that SA causes skeletal muscle weakness due to impaired EC coupling. This is likely to be induced by Ca2+-dependent degradation of triad proteins, which may result from Ca2+ leak from fragmented RyR1 triggered by increased oxidative stress. [ABSTRACT FROM AUTHOR]

Specific force (SF) has been shown to be reduced in some but not all studies of human aging using chemically skinned single muscle fibers. This may be due, in part, not only to the health status/physical activity levels of different older cohorts, but also from methodological differences in studying skinned fibers. The aim of the present study was to compare SF in fibers from older hip fracture patients (HFP), healthy master cyclists (MC), and healthy nontrained young adults (YA) using two different activating solutions. Quadriceps muscle samples and 316 fibers were obtained from HFPs (74.6 ± 4 years, n = 5), MCs (74.8 ± 1, n = 5), and YA (25.5 ± 2, n = 6). Fibers were activated (pCa 4.5, 15°C) in solutions containing either 60 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH buffer (TES) or 20 mM imidazole. SF was determined by normalizing force to fiber cross-sectional area (CSA) assuming either an elliptical or circular shape and to fiber myosin heavy chain content. Activation in TES resulted in significantly higher MHC-I SF in all groups and YA MHC-IIA fibers, irrespective of normalization method. Although there were no differences in SF between the participant groups, the ratio of SF between the TES and imidazole solutions was lower in HFPs compared with YAs (MHC-I P < 0.05; MHC-IIA P = 0.055). Activating solution composition, as opposed to donor characteristics, had a more notable effect on single fiber SF. However, this two-solution approach revealed an age-related difference in sensitivity in HFPs, which was not shown in MCs. This suggests further novel approaches may be required to probe age/activity-related differences in muscle contractile quality. [ABSTRACT FROM AUTHOR]

Age-related alterations of skeletal muscle are numerous and present inconsistently, and the effect of their interaction on contractile performance can be nonintuitive. Hill-type muscle models predict muscle force according to well-characterised contractile phenomena. Coupled with simple, yet reasonably realistic activation dynamics, such models consist of parameters that are meaningfully linked to fundamental aspects of muscle excitation and contraction. We aimed to illustrate the utility of a muscle model for elucidating relevant mechanisms and predicting changes in output by simulating the individual and combined effects on isometric force of several known ageing-related adaptations. Simulating literature-informed reductions in free Ca2+ concentration and Ca2+ sensitivity generated predictions at odds qualitatively with the characteristic slowing of contraction speed. Conversely, incorporating slower Ca2+ removal or a fractional increase in type I fibre area emulated expected changes; the former was required to simulate slowing of the twitch measured experimentally. Slower Ca2+ removal more than compensated for force loss arising from a large reduction in Ca2+ sensitivity or moderate reduction in Ca2+ release, producing realistic age-related shifts in the force-frequency relationship. Consistent with empirical data, reductions in free Ca2+ concentration and Ca2+ sensitivity reduced maximum tetanic force only slightly, even when acting in concert, suggesting a modest contribution to lower specific force. Lower tendon stiffness and slower intrinsic shortening speed slowed and prolonged force development in a compliance-dependent manner without affecting force decay. This work demonstrates the advantages of muscle modelling for exploring sources of variation and identifying mechanisms underpinning the altered contractile properties of aged muscle. [ABSTRACT FROM AUTHOR]

Using the finite element method, mathematical models were created and studies were carried out of direct and reverse methods of hot crimping in a matrix of a special profile of hollow workpieces made of high-carbon steel with a variable wall thickness along the height. A plastic model of metal and cylindrical coordinates are used. By calculation, the shape and dimensions of the initial workpiece for crimping were established, which provided the required dimensions of the product. Before crimping, a part of the billet was heated along the height, which was subject to deformation in the matrix. The use of a matrix of a special profile with a deforming surface made with annular protrusions made it possible to obtain a product in one step due to a decrease in the influence of friction forces during shaping. The deformation rate is determined to comply with the hot deformation mode during the crimping. The dependences of the axial force on the deforming tool on the movement of punches and the distribution of specific forces on the contact surfaces are established. The use of direct crimping leads to a decrease in forces modes and specific forces on the tool. The final stress-strain state of the metal, the shape and dimensions of the product are determined. According to the distribution of strain intensity, an assessment was made of the elaboration of the metal structure by plastic deformation. Based on the simulation data, a design of the general view of the die tooling for direct crimping has been developed. [ABSTRACT FROM AUTHOR]

Background: Specific force (maximal muscle force normalized to muscle size) is the single most valuable measure of the intrinsic health of a muscle. However, obtaining this measure accurately in humans is challenging since it requires painful high frequency nerve stimulation and time-consuming muscle imaging. Methods: Here, we evaluated the potential for predicting muscle specific force with needle electrical impedance myography (EIM) in healthy and muscular dystrophy mice. Results: Similar to the force-stimuli relationship, our results show the impedance increases with isometric contraction and that the magnitude of change depends on muscle activation. This impedance-force relationship was found to be different in healthy and diseased muscle supporting the idea that EIM can provide additional functional information currently unavailable with standard electrodiagnostic techniques. Conclusions: Needle EIM could greatly simplify the approach for obtaining an accurate measure of muscle specific force, leading to its wide application in future clinical neuromuscular disease research and care. [ABSTRACT FROM AUTHOR]

Abstract Background The extent of muscle deterioration associated with ageing or disease can be quantified by comparison with appropriate reference data. The objective of this study is to present normative data for lower-limb muscle strength and quality for 573 males and 923 females aged 20-97 yr participating in the Geelong Osteoporosis Study in southeastern Australia. Methods In this cross-sectional study, measures of muscle strength for hip flexors and hip abductors were obtained using a Nicholas manual muscle tester, a hand-held dynamometer (HHD; kg). Leg lean mass was measured by dual energy x-ray absorptiometry (DXA; kg), and muscle quality calculated as strength/mass (N/kg). Results For both sexes, muscle strength and quality decreased with advancing age. Age explained 12.9–25.3% of the variance in muscle strength in males, and 20.8–24.6% in females; age explained less of the variance in muscle quality. Means and standard deviations for muscle strength and quality for each muscle group are reported by age-decade for each sex, and cutpoints equivalent to T-scores of − 2.0 and − 1.0 were derived using data from young males (n = 89) and females (n = 148) aged 20–39 years. Conclusions These data will be useful for quantifying the extent of dynapenia and poor muscle quality among adults in the general population in the face of frailty, sarcopenia and other age-related muscle dysfunction.

Chemically skinned fibres allow the study of human muscle contractile function in vitro. A particularly important parameter is specific force (SF), that is, maximal isometric force divided by cross‐sectional area, representing contractile quality. Although SF varies substantially between studies, the magnitude and cause of this variability remains puzzling. Here, we aimed to summarize and explore the cause of variability in SF between studies. A systematic search was conducted in Medline, Embase and Web of Science databases in June 2020, yielding 137 data sets from 61 publications which studied healthy, young adults. Five‐fold differences in mean SF data were observed. Adjustments to the reported data for key methodological differences allowed between‐study comparisons to be made. However, adjustment for fibre shape, swelling and sarcomere length failed to significantly reduce SF variance (I2 = 96%). Interestingly, grouping papers based on shared authorship did reveal consistency within research groups. In addition, lower SF was found to be associated with higher phosphocreatine concentrations in the fibre activating solution and with Triton X‐100 being used as a skinning agent. Although the analysis showed variance across the literature, the ratio of SF in single fibres containing myosin heavy chain isoforms IIA or I was found to be consistent across research groups. In conclusion, whilst the skinned fibre technique is reliable for studying in vitro force generation of single fibres, the composition of the solution used to activate fibres, which differs between research groups, is likely to heavily influence SF values. [ABSTRACT FROM AUTHOR]

Partially filled pipes have many applications in hydraulic and environmental engineering; such as sewer and storm water collection pipes and circular culverts. Hydraulic jump can lead to scouring at hydraulic structures and thus excess energy should be dissipated to prevent scour and erosion. Calculation of conjugate depths in circular pipes and culverts is important in hydraulic engineering. The conjugate flow depths are commonly used to estimate the length of the hydraulic jump and the energy loss in the jump. Available relationships to estimate the conjugate depths in circular pipes are not accurate and wide application range. In comparison to easily available numerical solutions, a closed-form solution is a function of all input independent variables; and thus the dependence of the conjugate depth on each input independent variable can be easily interpreted. This study presents explicit solutions for the conjugate depths (pre- and post-jump depths) in partially filled pipes. The proposed solutions are examined over a wide range of the Froude number and geometric conditions, providing satisfactory estimations when compared with the exact solution obtained from the specific force relation. If the prejump flow depth of the hydraulic jump is known, the postjump flow depth can be determined using the proposed solution with a maximum relative error less than 1.4%. Inversely, if postjump flow depth is known, the prejump flow depth can be determined with a maximum relative error less than 2.3%. The proposed direct solutions are general, accurate, simple, and useful for studying the partially filled circular open channels. [ABSTRACT FROM AUTHOR]

A hydraulic jump is a well-known transitionalphenomenon from supercritical to subcritical flows withundulations of water-surface. In this transition, the water flowhas a high-velocity, water surface rises abruptly, surface rollersare formed, intense mixing occurs, the air is entrained, and alarge amount of energy is dissipated. In the present studycharacteristics of the hydraulic jumps in circular open channelswith a mild sloped have been discussed under a wide range ofexperimental conditions. The study aims to determine the effectof the channel’s mild slope on the characteristics of thehydraulic jumps with circular channels. A theoretical study hasbeen done and led to obtaining equations that can be used to getspecific force for the hydraulic jump that occurred in circularopen channels. A theoretical study is based on usingmomentum, and Froude’s number equations. An experimentalstudy has been investigated using Laboratory 40 experimentalruns in circular section flume for eight different dischargesranged from 3.92 to 12.07 l/sec were given with mild slopeequals 0.000833. In total, the experiments were conducted overa range of Froude numbers from 1.78 to 8.87. The experimentalworks concluded some dimensionless curves and new usefulformulas to get the length of the jump, energy dissipated andefficiency. The conjugated depth ratio is determined and plottedversus the upstream Froude number, downstream Froudenumber, head loss, and efficiency for different values of criticaldepths. The resulting graphs and equations of the present studyare readily applicable for a design for the hydraulic jumps inthe circular open channels with mild slope.

Background: Specific force, that is the amount of force generated per unit of muscle tissue, is reduced in patients with facioscapulohumeral muscular dystrophy (FSHD). The causes of reduced specific force and its relation with FSHD disease severity are unknown.Methods: Quantitative muscle magnetic resonance imaging (MRI), measurement of voluntary maximum force generation and quadriceps force-frequency relationship, and vastus lateralis muscle biopsies were performed in 12 genetically confirmed patients with FSHD and 12 controls.Results: Specific force was reduced by ~33% in all FSHD patients independent of disease severity. Quadriceps force-frequency relationship shifted to the right in severe FSHD compared to controls. Fiber type distribution in vastus lateralis muscle biopsies did not differ between groups.Conclusions: Reduced quadriceps specific force is present in all FSHD patients regardless of disease severity or fatty infiltration. Early myopathic changes, including fibrosis, and non-muscle factors, such as physical fatigue and musculoskeletal pain, may contribute to reduced specific force. [ABSTRACT FROM AUTHOR]

The kinetics and dynamics of the stiff and flexible tines with the duckfoot and the coulter after impact with stones embedded in compacted soil were examined. The beak of the duckfoot was positioned in the axis of the row of stones embedded in the soil at the depth of stones thickness. The coulter covered the stone or impact the edge of the stone halfway along its length. The tools worked at a speed of 0.83–2.22 m·s−1 and a working depth of 0.05–0.10 m. The results of specific parameters were compared to the response of the tools to loads in soil without stones. For both soil conditions, the kinetics of the flexible tine was 24 times more reactive, and the dynamic loads were two times lower than for the stiff tine. The responses of both tines were suppressed along with the working depth because of the more favorable place of impact of the duckfoot beak with the stone. Along with the working speed, for a stiff tine, the specific accelerations decreased significantly, by ten times, and the specific forces increased slightly, by 1.6 times. Among the two systems of setting the coulter, the impact of the cutting edge of the coulter with the stone in the middle of its length was more unfavorable than the work of the coulter covering the stone.

Background: The extent of muscle deterioration associated with ageing or disease can be quantified by comparison with appropriate reference data. The objective of this study is to present normative data for lower-limb muscle strength and quality for 573 males and 923 females aged 20-97 yr participating in the Geelong Osteoporosis Study in southeastern Australia.Methods: In this cross-sectional study, measures of muscle strength for hip flexors and hip abductors were obtained using a Nicholas manual muscle tester, a hand-held dynamometer (HHD; kg). Leg lean mass was measured by dual energy x-ray absorptiometry (DXA; kg), and muscle quality calculated as strength/mass (N/kg).Results: For both sexes, muscle strength and quality decreased with advancing age. Age explained 12.9-25.3% of the variance in muscle strength in males, and 20.8-24.6% in females; age explained less of the variance in muscle quality. Means and standard deviations for muscle strength and quality for each muscle group are reported by age-decade for each sex, and cutpoints equivalent to T-scores of - 2.0 and - 1.0 were derived using data from young males (n = 89) and females (n = 148) aged 20-39 years.Conclusions: These data will be useful for quantifying the extent of dynapenia and poor muscle quality among adults in the general population in the face of frailty, sarcopenia and other age-related muscle dysfunction. [ABSTRACT FROM AUTHOR]

Background. Both fasting and ageing processes lead to muscle wasting. We studied the effect of single 48 h fasting on soleus skeletal muscle mass and force in young and old mice. Methods. The study involved 18 young and 13 old mice. Body and soleus (SOL) muscle mass were measured followed by assessment of peak and specific SOL force under ex vivo conditions. SOL muscle half-relaxation time and 20 Hz to 100 Hz force ratio were also measured. Results. After fasting, weight loss was greater (p < .05) in young mice compared to old mice (17.0 ± 2.6 versus 12.0 ± 1.7%, respectively). Fasted young, but not old mice showed reduction (p < .05) in SOL mass compared to the control values. On the other hand, specific SOL force was reduced (p < .05) only in old mice, while 20 Hz to 100 Hz force ratio decreased (p < .05) only in young mice after fasting. Conclusions. Our results showed that 48 h fasting caused lower SOL muscle peak and specific force in old but not young mice. [ABSTRACT FROM AUTHOR]

Purpose. Parameters of the nominal mode are related to the most important performance indicators of traction means, therefore, the problems of choosing their optimal values always inevitably arise when forming technical requirements for a new rolling stock. The paper describes the features of solving the above-mentioned problems for electric trains with an asynchronous traction drive in the case of two-zone and three-zone frequency control of power. Methodology. Power of nominal mode of the rolling stock should be chosen in such a way that it would be possible to realize a predetermined travel time along in the section or the movement speed. On that basis, and also taking into account the fact that the important operational characteristics of electric trains include the acceleration value during the start-up and acceleration at the design speed, we will formulate the problem of determining the nominal power. In the task for a given range of traction, it is necessary to find such a value of the nominal mode power and the corresponding force value to ensure the ability to carry out transportations with the given level of average speed with minimal energy consumption for traction. At the same time, it is necessary to fulfill the following conditions: a) the speed of the electric train on the section does not exceed the established limits; b) it is possible to realize the given values of accelerations. A more detailed consideration of the problem shows that in real conditions, when the starting acceleration and the mass of the train are given, the problem of determining electric train power is practically reduced to determining the optimal value of the nominal mode speed. Findings. The task of choosing the optimal values of the nominal mode speed is solved by determining the electric power consumption with the variation of the possible values of starting speed. Therefore, only those values that ensure the implementation of the given starting and residual accelerations should be taken into account. The work shows that the traction force value increases with the design speed increase and other equal conditions, if the starting speed is increased. Originality. Authors developed the methodology for determining the optimal values of the nominal mode parameters of electric trains with an asynchronous traction drive, with two-zone and three-zone frequency power regulation. Practical value. The above mentioned methodology can be the basis when forming technical requirements for new rolling stock for Ukraine’s railways.

IntroductionTumor necrosis factor alpha (TNFα) is a pro-inflammatory cytokine that has been shown to modulate muscle mass, and is responsive to exercise training. The effects of resistance training (RT) followed by a short period of detraining on muscle size, architecture and function in combination with circulating TNFα levels have not been previously investigated in a young, healthy population.MethodsSixteen participants (8 males and 8 females) were randomly assigned to a training group (TRA; age 20 ± 3 years, mass 76 ± 7 kg), whilst fourteen participants (7 males and 7 females) age 22 ± 2 years, mass 77 ± 6 kg were assigned to a control group (CON). Measures of vastus lateralis (VL) muscle size (normalized physiological cross-sectional area allometrically scaled to body mass; npCSA), architecture (fascicle length; LF, pennation angle Pθ), strength (knee extensor maximal voluntary contraction; KE MVC), specific force, subcutaneous fat (SF) and circulating TNFα were assessed at baseline (BL), post 8 weeks RT (PT), and at two (DT1) and four (DT2) weeks of detraining.ResultsPooled BL TNFα was 0.87 ± 0.28 pg/mL with no differences between groups. BL TNFα tended to be correlated with npCSA (p = 0.055) and KEMVC (p = 0.085) but not specific force (p = 0.671) or SF (p = 0.995). There were significant (p < 0.05) increases in npCSA compared to BL and CON in TRA at PT, DT1, and DT2, despite significant (p < 0.05) decreases in npCSA compared to PT at DT1 and DT2. There were significant (p < 0.05) increases in LF, Pθ and KE MVC at PT but only LF and torque at DT1. There were no significant (p > 0.05) changes in SF, specific force or TNFα at any time points. There was a significant correlation (p = 0.022, r = 0.57) between the relative changes in TNFα and npCSA at DT2 compared to PT.DiscussionNeither RT nor a period of short term detraining altered the quality of muscle (i.e., specific force) despite changes in morphology and function. TNFα does not appear to have any impact on RT-induced gains in muscle size or function, however, TNFα may play a role in inflammatory-status mediated muscle mass loss during subsequent detraining in healthy adults.

New Findings: What is the central question of this study?Is the residual force generated by the diaphragm muscle after repeated activation reduced with sarcopenia, and is the residual force generated after fatiguing activation sufficient to sustain ventilatory behaviours of diaphragm muscle in young and old rats?What is the main finding and its importance?After diaphragm muscle fatigue, the residual specific force after 120 s of repeated stimulation was unaffected by ageing and was sufficient to accomplish ventilatory behaviours, but not expulsive manoeuvres (e.g. coughing). The inability to perform expulsive behaviours might underlie the increased susceptibility of older individuals to respiratory tract infections. Type IIx and/or IIb diaphragm muscle (DIAm) fibres make up more fatigable motor units that are more vulnerable to sarcopenia, i.e. age‐associated reductions of specific force and cross‐sectional area. In contrast, type I and IIa DIAm fibres form fatigue‐resistant motor units that are relatively unchanged with age. The fatigue resistance of the DIAm is assessed by normalizing the residual force generated after a period of repeated supramaximal stimulation (e.g. 120 s) to the initial maximal force. Given that sarcopenia primarily affects more fatigable DIAm motor units, apparent fatigue resistance improves with ageing. However, the central question is whether there is an ageing‐related difference in the residual force generated by the DIAm after repeated stimulation and whether this force is sufficient to sustain ventilatory behaviours of DIAm. In 6‐ and 24‐month‐old Fischer 344 rats, we assessed the loss of ex vivo DIAm force throughout 120 s of repeated supramaximal stimulation at 10, 40 and 75 Hz. We found that relative fatigue resistance improved in older rats at 40 and 75 Hz stimulation. Across all stimulation frequencies, DIAm residual force was unchanged with age (∼5 N cm−2). We conclude that ageing increases the relative contribution of type I and IIa fibres to DIAm force, with decreased contributions of type IIx and/or IIb fibres. The residual force generated by the DIAm after repeated stimulation is sufficient to accomplish ventilatory behaviours, regardless of age. [ABSTRACT FROM AUTHOR]

Introduction: Tumor necrosis factor alpha (TNFα) is a pro-inflammatory cytokine that has been shown to modulate muscle mass, and is responsive to exercise training. The effects of resistance training (RT) followed by a short period of detraining on muscle size, architecture and function in combination with circulating TNFα levels have not been previously investigated in a young, healthy population. Methods: Sixteen participants (8 males and 8 females) were randomly assigned to a training group (TRA; age 20 ± 3 years, mass 76 ± 7 kg), whilst fourteen participants (7 males and 7 females) age 22 ± 2 years, mass 77 ± 6 kg were assigned to a control group (CON). Measures of vastus lateralis (VL) muscle size (normalized physiological cross-sectional area allometrically scaled to body mass; npCSA), architecture (fascicle length; LF, pennation angle Pθ), strength (knee extensor maximal voluntary contraction; KE MVC), specific force, subcutaneous fat (SF) and circulating TNFα were assessed at baseline (BL), post 8 weeks RT (PT), and at two (DT1) and four (DT2) weeks of detraining. Results: Pooled BL TNFα was 0.87 ± 0.28 pg/mL with no differences between groups. BL TNFα tended to be correlated with npCSA (p = 0.055) and KEMVC (p = 0.085) but not specific force (p = 0.671) or SF (p = 0.995). There were significant (p < 0.05) increases in npCSA compared to BL and CON in TRA at PT, DT1, and DT2, despite significant (p < 0.05) decreases in npCSA compared to PT at DT1 and DT2. There were significant (p < 0.05) increases in LF, Pθ and KE MVC at PT but only LF and torque at DT1. There were no significant (p > 0.05) changes in SF, specific force or TNFα at any time points. There was a significant correlation (p = 0.022, r = 0.57) between the relative changes in TNFα and npCSA at DT2 compared to PT. Discussion: Neither RT nor a period of short term detraining altered the quality of muscle (i.e., specific force) despite changes in morphology and function. TNFα does not appear to have any impact on RT-induced gains in muscle size or function, however, TNFα may play a role in inflammatory-status mediated muscle mass loss during subsequent detraining in healthy adults. [ABSTRACT FROM AUTHOR]

Abstract Chronic kidney disease patients present with metabolic and functional muscle abnormalities, called uremic myopathy, whose mechanisms have not yet been fully elucidated. We investigated whether chronic renal insufficiency (CRI) affects skeletal muscle contractile properties at the cellular level. CRI was induced surgically in New Zealand rabbits (UREM), with sham-operation for controls (CON), and samples were collected at 3 months post-surgery, following euthanasia. All protocols had University Ethics approval following national and European guidelines. Sample treatments and evaluations were blinded. Maximal isometric force was assessed in 382 permeabilized psoas fibers (CON, n = 142, UREM, n = 240) initially at pH7, 10 °C ('standard' conditions), in subsets of fibers in acidic conditions (pH6.2, 10 °C) but also at near physiological temperature (pH7, 30 °C and pH6.2, 30 °C). CRI resulted in significant smaller average cross sectional areas (CSAs) by ∼11% for UREM muscle fibers (vs CON, P < 0.01). At standard conditions, UREM fibers produced lower absolute and specific forces (i.e. normalized force per fiber CSA) (vs CON, P < 0.01); force increased in 30 °C for both groups (P < 0.01), but the disparity between UREM and CON remained significant. Acidosis significantly reduced force (vs pH7, 10 °C P < 0.01), similarly in both groups (in UREM by −48% and in CON by −43%, P > 0.05). For the first time, we give evidence that CRI can induce significant impairments in single psoas muscle fibers force generation, only partly explained by fiber atrophy, thus affecting muscle mechanics at the cellular level. [ABSTRACT FROM AUTHOR]

Synergist ablation (SA) is an experimental procedure for the induction of hypertrophy. However, SA causes a decrease in specific force (i.e., force per cross-sectional area), likely due to excessive muscle use. Here, we investigated the mechanisms behind the SA-induced intrinsic contractile dysfunction, especially focusing on the excitation-contraction (EC) coupling. Male Wistar rats had unilateral surgical ablation of gastrocnemius and soleus muscles to induce compensatory hypertrophy in the plantaris muscles. Two weeks after SA, plantaris muscle was dissected from each animal and used for later analyses. SA significantly increased the mean fiber cross-sectional area (+18%). On the other hand, the ratio of depolarization-induced force to the maximum Ca 2+ -activated specific force, an indicator of sarcoplasmic reticulum (SR) Ca 2+ release, was markedly reduced in mechanically skinned fibers from the SA group (-51%). These functional defects were accompanied by an extensive fragmentation of the SR Ca 2+ release channel, the ryanodine receptor 1 (RyR1), and a decrease in the amount of other triad proteins (i.e., DHPR, STAC3, and junctophilin1). SA treatment also caused activation of calpain-1 and increased the amount of NADPH oxidase 2, endoplasmic reticulum (ER) stress proteins (i.e., Grp78, Grp94, PDI, and Ero1), and lipid peroxidation [i.e., 4-hydroxynonenal (4-HNE)] in SA-treated muscles. Our findings show that SA causes skeletal muscle weakness due to impaired EC coupling. This is likely to be induced by Ca 2+ -dependent degradation of triad proteins, which may result from Ca 2+ leak from fragmented RyR1 triggered by increased oxidative stress. NEW & NOTEWORTHY Synergist ablation (SA) has widely been used to understand the mechanisms behind skeletal muscle hypertrophy. However, compensatory hypertrophied muscles display intrinsic contractile dysfunction, i.e., a hallmark of overuse. Here, we demonstrate that SA-induced compensatory hypertrophy is accompanied by muscle weakness due to impaired sarcoplasmic reticulum Ca 2+ release. This dysfunction may be caused by the degradation of triad proteins due to the reciprocal amplification of reactive oxygen species and Ca 2+ signaling at the junctional space microdomain.

Achondroplasia is a clinical condition defined by shorter stature and disproportionate limb length. Force production in able-bodied individuals (controls) is proportional to muscle size, but given the disproportionate nature of achondroplasia, normalizing to anatomical cross-sectional area (ACSA) is inappropriate. The aim of this study was to assess specific force of the vastus lateralis (VL) in 10 adults with achondroplasia (22 ± 3 yr) and 18 sex-matched controls (22 ± 2 yr). Isometric torque (iMVCτ) of the dominant knee extensors (KE) and in vivo measures of VL muscle architecture, volume, activation, and patella tendon moment arm were used to calculate VL physiological CSA (PCSA), fascicle force, and specific force in both groups. Achondroplasic muscle volume was 53% smaller than controls (284 ± 36 vs. 604 ± 102 cm3, P < 0.001). KE iMVCτ was 63% lower in achondroplasia compared with controls (95 ± 24 vs. 256 ± 47 N⋅m, P < 0.001). Activation and moment arm length were similar between groups ( P > 0.05), but coactivation of bicep femoris of achondroplasic subjects was 70% more than controls (43 ± 20 vs. 13 ± 5%, P < 0.001). Achondroplasic subjects had 58% less PCSA (43 ± 10 vs. 74.7 ± 14 cm2, P < 0.001), 29% lower fascicle force (702 ± 235 vs. 1704 ± 303 N, P < 0.001), and 29% lower specific force than control subjects (17 ± 6 vs. 24 ± 6 N⋅cm-2, P = 0.012). The smaller VL specific force in achondroplasia may be attributed to infiltration of fat and connective tissue, rather than to any difference in myofilament function. NEW & NOTEWORTHY The novel observation of this study was the measurement of normalized force production in a group of individuals with disproportionate limb length-to-torso ratios. [ABSTRACT FROM AUTHOR]

Age-related declines in human skeletal muscle performance may be caused, in part, by decreased responsivity of muscle fibers to calcium (Ca 2+ ). This study examined the contractile properties of single vastus lateralis muscle fibers with various myosin heavy chain (MHC) isoforms (I, I/IIA, IIA and IIAX) across a range of Ca 2+ concentrations in 11 young (24.1 ± 1.1 years) and 10 older (68.8 ± 0.8 years) men and women. The normalized pCa-force curve shifted rightward with age, leading to decreased activation threshold (pCa 10 ) and/or Ca 2+ sensitivity (pCa 50 ) for all MHC isoforms examined. In older adults, the slope of the pCa-force curve was unchanged in MHC I-containing fibers (I, I/IIA), but was steeper in MHC II-containing fibers (IIA, IIAX), indicating greater cooperativity compared to young adults. At sub-maximal [Ca 2+ ], specific force was reduced in MHC I-containing fibers, but was minimally decreased in MHC IIA fibers as older adults produced greater specific forces at high [Ca 2+ ] in these fibers. Lessor pCa 50 in MHC I fibers independently predicted reduced isokinetic knee extensor power across a range of contractile velocities, suggesting that the Ca 2+ response of slow-twitch fibers contributes to whole muscle dysfunction. Our findings show that aging attenuates Ca 2+ responsiveness across fiber types and that these cellular alterations may lead to age-related reductions in whole muscle power output. [ABSTRACT FROM AUTHOR]

Laboratory rats are considered mature at 3 months despite that musculoskeletal growth is still occurring. Changes in muscle physiological and biochemical characteristics during development from 3 months, however, are not well understood. Whole muscles and single skinned fibres from fast-twitch extensor digitorum longus (EDL) and predominantly slow-twitch soleus (SOL) muscles were examined from male Sprague-Dawley rats (3, 6, 9, 12 months). Ca2+ sensitivity of contractile apparatus decreased with age in both fast- (~ 0.04 pCa units) and slow-twitch (~ 0.07 pCa units) muscle fibres, and specific force increased (by ~ 50% and ~ 25%, respectively). Myosin heavy chain composition of EDL and SOL muscles altered to a small extent with age (decrease in MHCIIa proportion after 3 months). Glycogen content increased with age (~ 80% in EDL and 25% in SOL) and GLUT4 protein density decreased (~ 35 and 20%, respectively), whereas the glycogen-related enzymes were little changed. GAPDH protein content was relatively constant in both muscle types, but COXIV protein decreased ~ 40% in SOL muscle. Calsequestrin (CSQ) and SERCA densities remained relatively constant with age, whereas there was a progressive ~ 2-3 fold increase in CSQ-like proteins, though their role and importance remain unclear. There was also ~ 40% decrease in the density of the Na+, K+-ATPase (NKA) α1 subunit in EDL and the α2 subunit in SOL. These findings emphasise there are substantial changes in skeletal muscle function and the density of key proteins during early to mid-adulthood in rats, which need to be considered in the design and interpretation of experiments. [ABSTRACT FROM AUTHOR]

Introduction: Muscle weakness determines functional impairment in spastic cerebral palsy (SCP). Measurement of specific force (SF) allows for strength comparison with unimpaired populations (controls) accounting for neural (activation and coactivation), architectural (fascicle length and pennation angle), and structural differences (moment arm length).Methods: Medial gastrocnemius (MG) SF (and its determinants) was assessed in both paretic and non-paretic legs of 11 men with SCP and 11 age-matched controls during plantarflexion maximal voluntary isometric contraction (MVIC).Results: SCP fascicles were 28% longer than control fascicles (P < 0.05). Pennation angle of SCP patients was 41% smaller than in controls. The physiological cross-sectional area of SCP MG patients was 47% smaller than in controls (P < 0.05). There was no difference in SF between controls and SCP patients.Conclusions: Weakness in SCP is primarily attributable to deficits in agonist activation and muscle size; consequently, SF measured in the MG is similar between SCP and controls. Muscle Nerve 56: 298-306, 2017. [ABSTRACT FROM AUTHOR]

Key points Loss of muscle mass and strength in the growing population of elderly people is a major health concern for modern societies. This condition, termed sarcopenia, is a major cause of falls and of the subsequent increase in morbidity and mortality., Despite numerous studies on the impact of ageing on individual muscle fibres, the contribution of single muscle fibre adaptations to ageing-induced atrophy and functional impairment is still unsettled., The level of physical function and disuse is often associated with ageing., We studied relatively healthy older adults in order to understand the effects of ageing per se without the confounding impact of impaired physical function., We found that in healthy ageing, structural and functional alterations of muscle fibres occur. Protein post-translational modifications, oxidation and phosphorylation contribute to such alterations more than loss of myosin and other muscle protein content., Abstract Contradictory results have been reported on the impact of ageing on structure and functions of skeletal muscle fibres, likely to be due to a complex interplay between ageing and other phenomena such as disuse and diseases. Here we recruited healthy, physically and socially active young (YO) and elderly (EL) men in order to study ageing per se without the confounding effects of impaired physical function. In vivo analyses of quadriceps and in vitro analyses of vastus lateralis muscle biopsies were performed. In EL subjects, our results show that (i) quadriceps volume, maximum voluntary contraction isometric torque and patellar tendon force were significantly lower; (ii) muscle fibres went through significant atrophy and impairment of specific force (isometric force/cross-sectional area) and unloaded shortening velocity; (iii) myosin/actin ratio and myosin content in individual muscle fibres were not altered; (iv) the muscle proteome went through quantitative adaptations, namely an up-regulation of the content of several groups of proteins among which were myofibrillar proteins and antioxidant defence systems; (v) the muscle proteome went through qualitative adaptations, namely phosphorylation of several proteins, including myosin light chain-2 slow and troponin T and carbonylation of myosin heavy chains. The present results indicate that impairment of individual muscle fibre structure and function is a major feature of ageing per se and that qualitative adaptations of muscle proteome are likely to be more involved than quantitative adaptations in determining such a phenomenon. [ABSTRACT FROM AUTHOR]

Background: Obesity alters whole body kinematics and joint kinetics during activities of daily living which are thought to contribute to increased risk of musculoskeletal injury, development of lower extremity joint osteoarthritis (OA), and physical disability. To date, it has widely been accepted that excess adipose tissue mass is the major driver of biomechanical alterations in obesity. However, it is well established that obesity is a systemic disease affecting numerous, if not all, organ systems of the body. Indeed, obesity elicits numerous adaptations within skeletal muscle, including alterations in muscle structure (ex. myofiber size, architecture, lipid accumulation, and fiber type), recruitment patterns, and contractile function (ex. force production, power production, and fatigue) which may influence kinematics and joint kinetics. This review discusses the specific adaptations of skeletal muscle to obesity, potential mechanisms underlying these adaptations, and how these adaptations may affect biomechanics. [ABSTRACT FROM AUTHOR]

Phenotypic diversity between laboratory mouse strains provides a model for studying the underlying genetic mechanisms. The A/J strain performs poorly in various endurance exercise models. The aim of the study was to test if endurance capacity and contractility of the fast- and slow-twitch muscles are affected by the genes on mouse chromosome 10. The C57BL/6J (B6) strain and C57BL/6J-Chr 10A/J/NaJ (B6.A10) consomic strain which carries the A/J chromosome 10 on a B6 strain background were compared. The B6.A10 mice compared to B6 were larger in body weight (p < 0.02): 27.2 ± 1.9 vs. 23.8 ± 2.7 and 23.4 ± 1.9 vs. 22.9 ± 2.3 g, for males and females, respectively, and in male soleus weight (p < 0.02): 9.7 ± 0.4 vs. 8.6 ± 0.9 mg. In the forced running test the B6.A10 mice completed only 64% of the B6 covered distance (p < 0.0001). However, there was no difference in voluntary wheel running (p = 0.6) or in fatigability of isolated soleus (p = 0.24) or extensor digitorum longus (EDL, p = 0.7) muscles. We conclude that chromosome 10 of the A/J strain contributes to reduced endurance performance. We also discuss physiological mechanisms and methodological aspects relevant to interpretation of these findings. [ABSTRACT FROM AUTHOR]

In this paper, a novel configuration of strategically distributed accelerometer sensors with the aid of one gyro to infer a flight vehicle’s angular motion is presented. The MEMS accelerometer and gyro sensors are integrated to form a gyroscope-reduced inertial measurement unit (GR-IMU). The motivation for gyro aided accelerometers array is to have direct measurements of angular rates, which is an improvement to the traditional gyroscope-free inertial system that employs only direct measurements of specific force. Some technical issues regarding error calibration in accelerometers and gyro in GR-IMU are put forward. The GR-IMU based inertial navigation system can be used to find a complete attitude solution for flight vehicle motion estimation. Results of numerical simulation are given to illustrate the effectiveness of the proposed configuration. The gyroscope-reduced inertial navigation system based on distributed accelerometer sensors can be developed into a cost effective solution for a fast reaction, MEMS based motion capture system. Future work will include the aid from external navigation references (e.g. GPS) to improve long time mission performance. [ABSTRACT FROM AUTHOR]