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15 results on '"Speight, Lee"'

1. Sustainable sustainability claims

Author

Speight, Lee Colyer, Lanci, Christopher J., and Mazeffa, Douglas

Subjects
Sustainable development, Raw materials, Green technology, Business, Chemicals, plastics and rubber industries
Abstract

Greenwashing, or the practice of misrepresenting or over representing the environmental benefits of a product or practice, is common in consumer marketing. The term was coined in 1986 by Jay [...]

Published
2015

2. Everything old is new again

Author

Speight, Lee Colyer

Subjects
Raw materials, Adhesives and sealants industry, Petroleum chemicals, Business, Chemicals, plastics and rubber industries
Abstract

This is a call for innovation and collaboration. The ascent--or, more appropriately--re-ascent of renewable chemistry provides great promise for the adhesives and sealants industry. However, the full promise of these [...]

Published
2015

5. Improving target amino acid selectivity in a permissive aminoacyl tRNA synthetase through counter-selection

Author

Sungwienwong, Itthipol, primary, Hostetler, Zachary M., additional, Blizzard, Robert J., additional, Porter, Joseph J., additional, Driggers, Camden M., additional, Mbengi, Lea Z., additional, Villegas, José A., additional, Speight, Lee C., additional, Saven, Jeffery G., additional, Perona, John J., additional, Kohli, Rahul M., additional, Mehl, Ryan A., additional, and Petersson, E. James, additional

Published
2017
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6. Efficient Synthesis and In Vivo Incorporation of Acridon-2-ylalanine, a Fluorescent Amino Acid for Lifetime and FRET/LRET Studies

Author

Speight, Lee C., Muthusamy, Anand K., Goldberg, Jacob M., Warner, John B., Wissner, Rebecca F., Willi, Taylor S., Woodman, Bradley F., Mehl, Ryan A., and Petersson, E. James

Subjects
Models, Molecular, Alanine, Luminescence, Fluorescence Resonance Energy Transfer, Acridines, Article
Abstract

The amino acid acridon-2-ylalanine (Acd) can be a valuable probe of protein conformational change because it is a long lifetime, visible wavelength fluorophore that is small enough to be incorporated during ribosomal biosynthesis. Incorporation of Acd into proteins expressed in Escherichia coli requires efficient chemical synthesis to produce large quantities of the amino acid and the generation of a mutant aminoacyl tRNA synthetase that can selectively charge the amino acid onto a tRNA. Here, we report the synthesis of Acd in 87% yield over five steps from Tyr and the identification of an Acd synthetase by screening candidate enzymes previously evolved from Methanococcus janaschii Tyr synthetase for unnatural amino acid incorporation. Furthermore, we characterize the photophysical properties of Acd, including quenching interactions with select natural amino acids and Förster resonance energy transfer (FRET) interactions with common fluorophores such as methoxycoumarin (Mcm). Finally, we demonstrate the value of incorporation of Acd into proteins, using changes in Acd fluorescence lifetimes, Mcm/Acd FRET, or energy transfer to Eu(3+) to monitor protein folding and binding interactions.

Published
2013

10. Efficient Synthesis and In Vivo Incorporation of Acridon-2-ylalanine, a Fluorescent Amino Acid for Lifetime and Förster Resonance Energy Transfer/Luminescence Resonance Energy Transfer Studies

Author

Speight, Lee C., primary, Muthusamy, Anand K., additional, Goldberg, Jacob M., additional, Warner, John B., additional, Wissner, Rebecca F., additional, Willi, Taylor S., additional, Woodman, Bradley F., additional, Mehl, Ryan A., additional, and Petersson, E. James, additional

Published
2013
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14. Sustainable Sustainability Claims.

Author

Colyer Speight, Lee, Lanci, Christopher J., and Mazeffa, Douglas

Subjects
GREENWASHING (Marketing), COATINGS industry, SURFACE coatings, RAW materials, MATERIALS
Abstract

The article focuses on the controversy over greenwashing of coatings and its impact on the industry. Topics discussed include greenwashing refers to misrepresenting the environmental advantages of a product or habit, a standard rule for assessing environmental effects of coatings applies on 10 percent for formulation, 40 percent for downstream usage, and 50 percent for raw material production, and sustainable raw material sourcing methods like book and claim, and mass balance.

Published
2015

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