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27. Energy metabolism and spermatogenesis

Author

Rotimi, Damilare Emmanuel, Iyobhebhe, Matthew, Oluwayemi, Elizabeth Temidayo, Olajide, Olasunkanmi Peter, Akinsanola, Bolanle Adenike, Evbuomwan, Ikponmwosa Owen, Asaleye, Rotdelmwa Maimako, and Ojo, Oluwafemi Adeleke

Published
2024
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46. Epigenetic aging of semen is associated with inflammation.

Author

Feng, Junxi, Rubbi, Liudmilla, Kianian, Reza, Mills, Jesse, Osadchiy, Vadim, Sigalos, John, Eleswarapu, Sriram, and Pellegrini, Matteo

Subjects
Epigenetics, aging, male infertility, semen, sperm, Humans, Male, Epigenesis, Genetic, Inflammation, Aging, Adult, DNA Methylation, Semen, Middle Aged, Infertility, Male, Epigenome
Abstract

Male infertility has been a primary cause of global infertility, affecting 8-12% of couples worldwide. Previous studies have shown that semen quality decreases with advanced aging with an increased presence of inflammatory cells. In this study, we examined changes in the epigenome across a diverse cohort that includes both fertile and infertile men. We also compare the age-associated changes in semen to those observed in buccal swabs in order to characterize differences in epigenetic aging across diverse tissues. We found that variations in the semen methylome associated with aging are linked to inflammatory genes. Many age-associated sites are demethylated with advanced aging and are associated with the activation of inflammatory pathways. By contrast, we do not observe age-associated changes in inflammatory genes in buccal swab methylomes, which instead are characterized by changes to bivalent promoters. Our findings highlight the potential of epigenetic markers as indicators of male reproductive health.

Published
2024

49. Which is more effective in thawing frozen rooster sperm: varying temperature or duration?

Author

Nizam, Mustafa Yiğit, Selçuk, Murat, and Esin, Burcu

Subjects
FROZEN semen, POULTRY breeding, UNSATURATED fatty acids, SPERM motility, THAWING
Abstract

Introduction: Cryopreservation of poultry sperm is crucial for preserving genetic diversity and protecting endangered breeds. Rooster sperm is highly sensitive to cryopreservation due to its high polyunsaturated fatty acid content, making it prone to damage during freezing and thawing. This study evaluated the effects of thawing temperatures and storage conditions on sperm quality, including motility, morphology, and viability. Methods: Frozen rooster semen samples were thawed at 37°C for 30 seconds, 60°C for 5 seconds, or 72°C for 5 seconds and stored at 4°C for up to 48 hours. Sperm quality parameters, including motility, kinematic characteristics, abnormal morphology, and viability, were assessed at 0, 3, 6, 9, 12, 24, and 48 hours using a Computer-Assisted Semen Analyzer (CASA). Results: Post-thaw motility varied significantly between thawing temperatures at 24 and 48 hours (p < 0.05). Progressive and rapid progressive motility also differed significantly at 24 hours (p < 0.05). Sperm viability showed statistical differences across thawing groups at 24 and 48 hours (p < 0.05), while morphological abnormalities were significant at 12 and 48 hours (p < 0.05). Across all groups, sperm quality parameters varied significantly at each time point (p < 0.05). Discussion: Thawing at 37°C and storing at 4°C for up to 24 hours optimizes sperm motility and viability, minimizing cryodamage and ensuring functional preservation. This approach is effective for short-term storage and crucial for sustaining genetic diversity and fertility in poultry breeding programs. [ABSTRACT FROM AUTHOR]

Published
2025
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50. Comparison of Methods for Rapid Determination of Cholesterol Concentration in Human Sperm Membrane in Clinical Laboratory Practice.

Author

Mironova, A. G., Afanasyeva, S. I., Sybachin, A. V., Spiridonov, V. V., Bolshakov, M. A., and Simonenko, E. Yu.

Abstract

Objective: Cholesterol is an important structural component of the plasma membrane of mammalian cells. Cholesterol, among other important roles, plays a special role in sperm membranes. Change in the lipid composition of the sperm membrane, particularly the outflow of cholesterol, is an integral part of the process of capacitation and subsequent acrosomal reaction necessary for the sperm to fertilize an egg. Deviations in cholesterol concentration in sperm membrane may indicate a decrease in the fertilizing potential of sperm. To determine the optimal method for rapid analysis of the cholesterol content in human sperm membranes in the IVF laboratory, four methods of quantitative determination of cholesterol were compared in terms of practicality and effectiveness of their use to assess the concentration of cholesterol in human sperm membranes: the method of enzymatic colorimetric detection, the Lieberman–Burchard method, infrared spectroscopy and high-performance liquid chromatography. Methods: 101 ejaculates of patients with established normozoospermia (according to WHO criteria) were used in the work. Spermatozoa were separated from the semen by double centrifugation with the addition of DPBS medium. The resulting cellular pellet was used to determine the concentration of cholesterol in a sample by one of four methods: enzymatic colorimetric detection (FCD), HPLC, Lieberman-Burchard method, infrared spectroscopy. Results and Discussion: The following cholesterol concentrations were obtained by enzymatic colorimetric detection, Lieberman–Burchard, infrared spectroscopy and high-performance liquid chromatography: 1.0 ± 0.3, 1.32 ± 0.15, 5.1 ± 1.8 and 1.53 ± 0.18 nmol/106 cells, respectively. The Lieberman–Burchard method, enzymatic colorimetric detection and HPLC showed similar results, the obtained average cholesterol concentrations coincide within the error. The mean cholesterol concentration in sperm membranes obtained using infrared spectroscopy method significantly exceeds the values presented in the literature and the values obtained using other methods. In addition, this method requires an amount of analyzed material that significantly exceeds the volume of one ejaculate. Conclusions: As a result of comparing four methods of quantitative cholesterol analysis, the method of enzymatic colorimetric detection is proposed as a method of rapid analysis of cholesterol in human sperm membranes suitable for routine use in a clinical laboratory. The advantages of this method include the low toxicity of the method, it's cost-effectiveness and a significant reduction in the time of complete analysis: from sample preparation to obtaining the result. [ABSTRACT FROM AUTHOR]

Published
2025
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