Search

Your search keyword '"Stabellini G."' showing total 324 results
Start Over Author "Stabellini G."
324 results on '"Stabellini G."'

6. Extracellular matrix and growth factors in the pathogenesis of some craniofacial malformations

Author

Carinci, P., Becchetti, E., Baroni, T., Carinci, F., Pezzetti, F., Stabellini, G., Locci, P., Luca Scapoli, Tognon, M., Volinia, S., Bodo, M., Carinci P, Becchetti E, Baroni T, Carinci F, Pezzetti F, Stabellini G, Locci P, Scapoli L, Tognon M, Volinia S, and Bodo M.

Subjects
Craniofacial Abnormalities, Humans, FIBROBLAST GROWTH FACTOR RECEPTOR, Growth Substances, CRANIOSYNOSTOSIS, Extracellular Matrix
Abstract

The normal development of cranial primordia and orofacial structures involves fundamental processes in which growth, morphogenesis, and cell differentiation take place and interactions between extracellular matrix (ECM) components, growth factors and embryonic tissues are involved. Biochemical and molecular aspects of craniofacial development, such as the biological regulation of normal or premature cranial suture fusion, has just begun to be understood, thanks mainly to studies performed in the last decade. Several mutations has been identified in both syndromic and non-syndromic craniosynostosis patients throwing new light onto the etiology, classification and developmental pathology of these diseases. In the more common craniosynostosis syndromes and other skeletal growth disorders, the mutations were identified in the genes encoding fibroblast growth factor receptor types 1-3 (FGFR1, 2 and 3) where they are dominantly acting and affect specific and important protein binding domain. The unregulated FGF signaling during intramembranous ossification is associated to the Apert and Crouzon syndrome. The non syndromic cleft of the lip and/or palate (CLP) has a more complex genetic background if compared to craniosynostosis syndrome because of the number of involved genes and type of inheritance. Moreover, the influence of environmental factor makes difficult to clarify the primary causes of this malformation. ECM represents cell environment and results mainly composed by collagens, fibronectin, proteoglycans (PG) and hyaluronate (HA). Cooperative effects of ECM and growth factors regulate regional matrix production during the morphogenetic events, connective tissue remodelling and pathological states. In the present review we summarize the studies we performed in the last years to better clarify the role of ECM and growth factors in the etiology and pathogenesis of craniosynostosis and CLP diseases.

Published
2007

7. Lung regions differently modulate bronchial branching development and extracellular matrix plays a role in regulating the development of chick embryo whole lung

Author

Stabellini, G., Calvitti, M., Becchetti, E., Carinci, P., Calastrini, C., Lilli, C., ROSSELLA SOLMI, Vizzotto, L., Baroni, T., Stabellini G., Calvitti M., Becchetti E., Carinci P., Calastrini C., Lilli C., Solmi R., Vizzotto L., and Baroni T.

Subjects
Settore BIO/17 - Istologia, Settore BIO/16 - Anatomia Umana, Hyaluronoglucosaminidase, Bronchi, Chick Embryo, respiratory system, Chondroitin ABC Lyase, CHICK EMBRIO, respiratory tract diseases, Extracellular Matrix, DEVELOPMENT, Organ Culture Techniques, lcsh:Biology (General), Acetylglucosaminidase, Animals, lcsh:QH301-705.5, Lung
Abstract

Normal branching development is dependent on the correlation between cells and extracellular matrix. In this interaction glycosaminoglycans, cytokines and growth factors play a fundamental role. In order to verify the distribution and influence of extracellular matrix and related enzymes on chick embryo lung development, 6 day-old whole lungs were maintained in vitro with testicular hyaluronidase, beta-N-acetyl-D-glucosaminidase and chondrotinase ABC or in linkage with apical, medial and caudal lung regions of 6-day development before and after enzyme treatment. In a separate lung region beta-N-acetyl-D-glucosaminidase and hyaluronidase were determined. Our data show that the whole lung cultures increase bronchial branching development when the medial region is admixed separately, while the separate apical or caudal regions or apical combined with caudal region do not affect bronchial branching development. The enzyme treatment of medial region prevents the branching development in associated whole lung. The bronchial branching development of whole lung cultured in medium containing the enzymes related to glycosaminoglycans turnover is significantly altered. In conclusion, these data show that the different influence of separate apical, medial, caudal lung regions on bronchial branching development is related to the extracellular matrix composition.

Published
2007

8. I tessuti del dente

Author

BUSCEMI, Maria, GERBINO, Aldo, STABELLINI G., BUSCEMI M, GERBINO A, and STABELLINI G

Published
2007

9. Bronchial branching correlates with specific glycosidase activity, extracellular glycosaminoglycan accumulation, TGF beta(2), and IL-1 localization during chick embryo lung development

Author

Calvitti, Mario, Baroni, Tiziano, Calastrini, C., Lilli, Cinzia, Caramelli, E., Becchetti, Ennio, Carinci, P., Vizzotto, L., Stabellini, G., CALVITTI M, BARONI T, CALASTRINI C, LILLI C, CARAMELLI E., BECCHETTI E, CARINCI P, VIZZOTTO L, and STABELLINI G.

Subjects
carbohydrates (lipids), INTERLEUKIN-1 (IL-1), IL-1, Bronchial branching. Glycosidase. Glycosaminoglycans. TGF beta(2), BRONCHIAL DEVELOPMENT, chick embryo, lung development, TRANSFORMING GROWTH FACTOR BETA 2 (TGF BETA 2), CELL-EXTRACELLULAR MATRIX (ECM), PROTEOGLYCANS
Abstract

During organ differentiation, cell-extracellular matrix (ECM) interactions are required. The components of the ECM, such as glycosaminoglycans, fibronectin, laminin, and collagens, change in relation to cytokine and enzyme activity. Moreover, glycosaminoglycans (GAGs) are components of the ECM that play an important role in both cytokine regulation and cell activities. In this work we studied the accumulation of hyaluronic acid and chondroitin sulfate and heparan sulfate proteoglycans (PGs), beta-N-acetyl-D-glucosaminidase activity, the presence of transforming growth factor beta(2) (TGF beta(2)), and interleukin-1 (IL-1), and the localization of fibronectin, laminin, and collagen I and IV during the early stages of chick embryo lung development. We also determined the levels of hyaluronic acid, chondroitin sulfate, dermatan sulfate, and heparan sulfate GAGs and the activity of beta-N-acetyl-D-glucosaminidase with biochemical methods. Our data show that beta-N-acetyl-D-glucosaminidase activity increases in each cell, especially in the epithelial growth front at the emergence of each bronchial bud, where hyaluronic acid and IL-1 are located in the surrounding mesenchymal areas. Chondroitin sulfate and heparan sulfate PGs, fibronectin, laminin, and collagen I and IV are evident in the area near the basal membrane along the sides where the forming structures are stabilized. Biochemical data show that beta-N-acetyl-D-glucosaminidase activity increases in cells during lung development and is related to GAG decrease and to modifications of the nonsulfated/sulfated GAG ratio. These modifications could change cytokine activity and play an important role in bronchial

Published
2004

13. Corrigendum to 'Patterns of some extracellular matrix gene expression are similar in cells from cleft lip-palate patients and in human palatal fibroblasts exposed to diazepam in culture' [Toxicology 257 (2009) 10-16] (DOI:10.1016/j.tox.2008.12.002)

Author

Marinucci, L., Balloni, S., Bodo, M., Carinci, F., Pezzetti, F., Stabellini, G., Conte, C., and Lumare, E.

Subjects
Diazepam, Cleft palate fibroblasts, ECM proteins, Integrins, Metalloproteases, FGF2, TGF-beta, GABRB3, Integrins, Diazepam, ECM proteins, FGF2, GABRB3, Metalloproteases, Cleft palate fibroblasts, TGF-beta
Published
2009

40. DEHP-induced alterations in the lining tissue of the rat air pouch

Author

Stabellini, G., Bedani, P. L., Fiocchi, O., Calastrini, C., Pagliarini, A., Lunghi, M., Carinci, F., Pellati, Agnese, Giuliani, Anna Lisa, and Berti, G.

Subjects
Cytoplasm, analysis, Wistar, Apoptosis, Fibroblasts, Administration, Cutaneous, Animals, Apoptosis, drug effects, Coloring Agents, Culture Techniques, methods, Cytoplasm, drug effects/ultrastructure, Diethylhexyl Phthalate, administration /&/ dosage/toxicity, Fibroblasts, drug effects/ultrastructure, Glycosaminoglycans, analysis, Peritoneal Dialysis, Continuous Ambulatory, methods, Rats, Rats, Wistar, Skin, drug effects/ultrastructure, methods, Rats, administration /&/ dosage/toxicity, Cutaneous, drug effects, Culture Techniques, Diethylhexyl Phthalate, Animals, Coloring Agents, Peritoneal Dialysis, Glycosaminoglycans, Skin
Published
1998

47. Effects of spermidine synthase inhibition on cytoskeletal organization in cultured chick embryo fibroblasts

Author

Caruso, A., Pellati, A., Giampaolo Bosi, Arena, N., and Stabellini, G.

Subjects
antagonists /&/ inhibitors, Cyclohexylamines, Cultured, Cells, Actin Cytoskeleton, ultrastructure, Actins, ultrastructure, Animals, Cells, Cultured, Chick Embryo, Colchicine, pharmacology, Cyclohexylamines, pharmacology, Cytoskeleton, ultrastructure, Fibroblasts, drug effects/ultrastructure, Microtubules, ultrastructure, Spermidine Synthase, antagonists /&/ inhibitors, Tubulin, ultrastructure, Chick Embryo, Fibroblasts, Microtubules, Spermidine Synthase, Actins, Tubulin, drug effects/ultrastructure, Animals, pharmacology, Colchicine, Cytoskeleton
Published
1994

Catalog

Books, media, physical & digital resources
See catalog results