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16 results on '"Stals, Hilde"'

2. The Cyclin-Dependent Kinase Inhibitor KRP6 Induces Mitosis and Impairs Cytokinesis in Giant Cells Induced by Plant-Parasitic Nematodes in Arabidopsis

Author

Vieira, Paulo, De Clercq, Annelies, Stals, Hilde, Van Leene, Jelle, Van De Slijke, Eveline, Van Isterdael, Gert, Eeckhout, Dominique, Persiau, Geert, Van Damme, Daniël, Verkest, Aurine, de Souza, José Dijair Antonino, Glab, Nathalie, Abad, Pierre, Engler, Gilbert, Inzé, Dirk, De Veylder, Lieven, De Jaeger, Geert, and de Almeida Engler, Janice

Published
2014

5. 2D-CEX–FcRn–MS to Study Structure/Function Relation of mAb Charge Variants

Author

Verscheure, Liesa, Vandenheede, Isabel, De Rore, Eline, Meersseman, Mabelle, Hanssens, Valerie, Meerschaert, Kris, Stals, Hilde, Sandra, Pat, Lynen, Frederic, Borgions, Filip, and Sandra, Koen

Abstract

The automated elucidation of the interplay between monoclonal antibody (mAb) structure and function using two-dimensional liquid chromatography–mass spectrometry (2D-LC–MS) is reported. Charge variants, induced through forced degradation, are resolved by first-dimension (1D) cation-exchange chromatography (CEX) and subsequently collected in loops installed on a multiple heart-cutting valve prior to transfer to second-dimension (2D) neonatal crystallizable fragment receptor (FcRn) affinity chromatography coupled with MS. As such, binding affinity of the latter mAb variants can elegantly be assessed and a first glimpse of identity provided. To maximize MS sensitivity, charge variants are unfolded upon eluting from the 2D affinity column by postcolumn addition of a denaturing solution. Further structural details, i.e., modification sites and chain distribution, are unraveled by a multidimensional LC–MS (mD-LC–MS) setup incorporating 1D CEX and parallel online middle-up and bottom-up LC–MS analysis in the subsequent dimensions. Identified charge variants could be ranked according to their affinity for FcRn. Binding is predominantly impacted by heavy chain (HC) M253oxidation and to a lesser extend, M429oxidation. Oxidation of both HCs more drastically affects FcRn interaction compared to single-chain oxidation, and the more oxidation, the less binding. Other modifications, such as HC glycosylation, HC N385/390, and N326deamidation or HC C-terminal processing, are not shown to affect binding. The streamlined platform is challenged against the established workflow involving offline collection of charge variants and structural and functional assessment by, respectively, LC–MS and enzyme-linked immunosorbent assay (ELISA). A decent correlation is demonstrated between the binding affinity measured with ELISA and 2D FcRn affinity chromatography. In addition, throughput is improved (7-fold), material requirements are substantially reduced (2 orders of magnitude), and sample preparation artifacts and loss are minimized. With the simultaneous determination of mAb structure and function, the current study takes the concept of multiattribute analysis to the next level, thereby contributing to the future development of safer and more effective antibody therapeutics.

Published
2024
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6. Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana

Author

Van Leene, Jelle, primary, Hollunder, Jens, additional, Eeckhout, Dominique, additional, Persiau, Geert, additional, Van De Slijke, Eveline, additional, Stals, Hilde, additional, Van Isterdael, Gert, additional, Verkest, Aurine, additional, Neirynck, Sandy, additional, Buffel, Yelle, additional, De Bodt, Stefanie, additional, Maere, Steven, additional, Laukens, Kris, additional, Pharazyn, Anne, additional, Ferreira, Paulo C G, additional, Eloy, Nubia, additional, Renne, Charlotte, additional, Meyer, Christian, additional, Faure, Jean‐Denis, additional, Steinbrenner, Jens, additional, Beynon, Jim, additional, Larkin, John C, additional, Van de Peer, Yves, additional, Hilson, Pierre, additional, Kuiper, Martin, additional, De Veylder, Lieven, additional, Van Onckelen, Harry, additional, Inzé, Dirk, additional, Witters, Erwin, additional, and De Jaeger, Geert, additional

Published
2010
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7. A bioanalytical method for the proteome wide display and analysis of protein complexes from whole plant cell lysates

Author

Remmerie, Noor, primary, Roef, Luc, additional, Van De Slijke, Eveline, additional, Van Leene, Jelle, additional, Persiau, Geert, additional, Eeckhout, Dominique, additional, Stals, Hilde, additional, Laukens, Kris, additional, Lemière, Filip, additional, Esmans, Eddy, additional, Van Onckelen, Harry, additional, Inzé, Dirk, additional, De Jaeger, Geert, additional, and Witters, Erwin, additional

Published
2009
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8. A Tandem Affinity Purification-based Technology Platform to Study the Cell Cycle Interactome in Arabidopsis thaliana

Author

Van Leene, Jelle, primary, Stals, Hilde, additional, Eeckhout, Dominique, additional, Persiau, Geert, additional, Van De Slijke, Eveline, additional, Van Isterdael, Gert, additional, De Clercq, Annelies, additional, Bonnet, Eric, additional, Laukens, Kris, additional, Remmerie, Noor, additional, Henderickx, Kim, additional, De Vijlder, Thomas, additional, Abdelkrim, Azmi, additional, Pharazyn, Anne, additional, Van Onckelen, Harry, additional, Inzé, Dirk, additional, Witters, Erwin, additional, and De Jaeger, Geert, additional

Published
2007
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10. A technology platform for the fast production of monoclonal recombinant antibodies against plant proteins and peptides

Author

Eeckhout, Dominique, primary, De Clercq, Annelies, additional, Van De Slijke, Eveline, additional, Van Leene, Jelle, additional, Stals, Hilde, additional, Casteels, Peter, additional, Persiau, Geert, additional, Vercammen, Dominique, additional, Van Breusegem, Frank, additional, Zabeau, Marc, additional, Inzé, Dirk, additional, Jespers, Laurent, additional, Depicker, Ann, additional, and De Jaeger, Geert, additional

Published
2004
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14. Cyclin-Dependent Kinase Inhibitor KRP6 Induces Mitosis and Impairs Cytokinesis in Giant Cells Induced by Plant-Parasitic Nematodes in Arabidopsis.

Author

Vieira, Paulo, Clercq, Annelies De, Stals, Hilde, Leene, Jelle Van, Slijke, Eveline Van De, Isterdael, Gert Van, Eeckhout, Dominique, Persiau, Geert, Damme, Daniël Van, Verkest, Aurine, Souza, José Dijair Antonino de, Júnior, Glab, Nathalie, Abad, Pierre, Engler, Gilbert, Inzé, Dirk, Veylder, Lieven De, Jaeger, Geert De, and Engler, Janice de Almeida

Subjects
CYCLIN-dependent kinase inhibitors, CELL cycle, CYTOKINESIS, NEMATODES, MITOSIS, PLANT nematodes, SOUTHERN root-knot nematode, NUCLEAR membranes
Abstract

In Arabidopsis thaliana , seven cyclin-dependent kinase (CDK) inhibitors have been identified, designated interactors of CDKs or Kip-related proteins (KRPs). Here, the function of KRP6 was investigated during cell cycle progression in roots infected by plant-parasitic root-knot nematodes. Contrary to expectations, analysis of Meloidogyne incognita –induced galls of KRP6 -overexpressing lines revealed a role for this particular KRP as an activator of the mitotic cell cycle. In accordance, KRP6 -overexpressing suspension cultures displayed accelerated entry into mitosis, but delayed mitotic progression. Likewise, phenotypic analysis of cultured cells and nematode-induced giant cells revealed a failure in mitotic exit, with the appearance of multinucleated cells as a consequence. Strong KRP6 expression upon nematode infection and the phenotypic resemblance between KRP6 overexpression cell cultures and root-knot morphology point toward the involvement of KRP6 in the multinucleate and acytokinetic state of giant cells. Along these lines, the parasite might have evolved to manipulate plant KRP6 transcription to the benefit of gall establishment. [ABSTRACT FROM AUTHOR]

Published
2014
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16. Jasmonic acid prevents the accumulation of cyclin B1;1 and CDK-B in synchronized tobacco BY-2 cells

Author

Řwiątek, Agnieszka, Azmi, Abdelkrim, Stals, Hilde, Inzé, Dirk, and Van Onckelen, Harry

Subjects
PROTEIN kinases, PLANT growth, MERISTEM culture, MITOSIS
Abstract

Jasmonic acid (JA) plays a crucial role in plant fertility and defense responses. It exerts an inhibitory effect on plant growth when applied exogenously. This effect seems to be somehow related to a negative regulation of cell cycle progression in the meristematic tissues. In this report, we focus on the molecular events that occur during JA-induced G2 arrest. We demonstrate that JA prevents the accumulation of B-type cyclin-dependent kinases and the expression of cyclin B1;1, which are both essential for the initiation of mitosis. This feature suggests the existence of an early G2 checkpoint that is affected by JA. [Copyright &y& Elsevier]

Published
2004
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