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Human induced pluripotent stem cell (iPSC) lines were generated from peripheral blood mononuclear cells (PBMCs) isolated from two related patients diagnosed with either idiopathic ventricular fibrillation or catecholaminergic polymorphic ventricular tachycardia, carrying an unknown variant in the RYR2 gene, c.14201A>G (p.Y4734C) and one healthy related individual. Reprogramming was done using a commercially available Epi5 Reprogramming Kit. The pluripotency of the iPSC lines was verified by the expression of pluripotency markers and by their capacity to differentiate into all three embryonic germ layers in vitro. These iPSC lines are available for functional analysis and in vitro studies of RYR2 channelopathy.

Countries of the European Union have stated transition to carbon-neutral economy until the year of 2050. Countries with a higher share of coal-fired power generation currently have no solution to end their combustion and use clean, emission-free energy immediately. The solution to this problem in the energy industry appears to be the increased use of natural gas, which significantly reduces CO2 emissions. In this article, we investigated the possibility of using coal in situ, using UCG (underground coal gasification) technology. We focused on verified geological, hydrogeological, and tectonic information about the selected brown coal deposit in Slovakia. This information has been assessed in research projects in recent years at the Technical University. From the abovementioned information, possible adverse factors were evaluated. These factors affect the rock environment around the underground generator by UCG activity. As part of the process management, measures were proposed to eliminate the occurrence of pollution and adverse effects on the environment. In the final phase of the UCG technology, we proposed to carry out, in the boreholes and in the generator cavity, water flushing and subsequent grouting. The proposed are suitable materials for solidification and stabilization. Results of this article´s solutions are crucial in the case of usage of this so-called clean technology, not only in Slovakia but also worldwide.

A main measure of the appraisal of the mining- building impact to the environment is the appraisal of the harmful materials extracted from the fill mixture into mining water. The presented article deals with geochemical measures of physical and chemical properties of the fill mixture, and the hydrogeological and geochemical characteristics of filling. Presently, a more increasing interest is in the exploitation of the utilization of high- capacity wastes in the mining environment.