Search

Remote sensing methods enable detection of solar-induced chlorophyll a fluorescence. However, to unleash the full potential of this signal, intensive cross-disciplinary work is required to harmonize biophysical and ecophysiological studies. For decades, the dynamic nature of chlorophyll a fluorescence (ChlaF) has provided insight into the biophysics and ecophysiology of the light reactions of photosynthesis from the subcellular to leaf scales. Recent advances in remote sensing methods enable detection of ChlaF induced by sunlight across a range of larger scales, from using instruments mounted on towers above plant canopies to Earth-orbiting satellites. This signal is referred to as solar-induced fluorescence (SIF) and its application promises to overcome spatial constraints on studies of photosynthesis, opening new research directions and opportunities in ecology, ecophysiology, biogeochemistry, agriculture and forestry. However, to unleash the full potential of SIF, intensive cross-disciplinary work is required to harmonize these new advances with the rich history of biophysical and ecophysiological studies of ChlaF, fostering the development of next-generation plant physiological and Earth-system models. Here, we introduce the scale-dependent link between SIF and photosynthesis, with an emphasis on seven remaining scientific challenges, and present a roadmap to facilitate future collaborative research towards new applications of SIF.

Photosynthetic light-harvesting antennae are pigment-binding proteins that perform one of the most fundamental tasks on Earth, capturing light and transferring energy that enables life in our biosphere. Adaptation to different light environments led to the evolution of an astonishing diversity of light-harvesting systems. At the same time, several strategies have been developed to optimize the light energy input into photosynthetic membranes in response to fluctuating conditions. The basic feature of these prompt responses is the dynamic nature of antenna complexes, whose function readily adapts to the light available. High-resolution microscopy and spectroscopic studies on membrane dynamics demonstrate the crosstalk between antennae and other thylakoid membrane components. With the increased understanding of light-harvesting mechanisms and their regulation, efforts are focusing on the development of sustainable processes for effective conversion of sunlight into functional bio-products. The major challenge in this approach lies in the application of fundamental discoveries in light-harvesting systems for the improvement of plant or algal photosynthesis. Here, we underline some of the latest fundamental discoveries on the molecular mechanisms and regulation of light harvesting that can potentially be exploited for the optimization of photosynthesis.

The connection between solar-induced fluorescence (SIF) and vegetation gross primary productivity is being widely investigated across spatial, temporal, and biological scales, including: a) studies at the leaf [1], [2], plant canopy [2]–[4] or satellite pixel scale [5], [6], b) temporally with studies spanning from diurnal [7] to seasonal scales [1], [3], [5], and b) biologically with studies covering various plant functional types (PFTs), e.g., crops [4], [7], deciduous [8] or evergreen forests [1], [3], in response to different sources of stress.

Photosystem I (PSI) has evolved in anaerobic atmospheric conditions and until today remains susceptible to oxygen. To minimize the probability of damaging side reactions, plants have evolved sophisticated mechanisms to control electron transfer, and PSI becomes inhibited only when malfunctions of these regulatory mechanisms occur. Because of the complicated induction of PSI photoinhibition, a detailed investigation into the process and following reactions are still largely missing. Here, we introduce the theoretical framework and a novel method for an easy and controlled induction of PSI photoinhibition in vivo. The method mimics the PSI damage mechanisms of fluctuating light-sensitive mutant plants (stn7, pgr5) which cannot control electron donation to PSI. Because PSII and PSI have different light absorption properties, electrons accumulate in the intersystem electron transfer chain (ETC), if PSII is preferentially excited. A saturating light pulse given upon an over-reduced ETC leads to the saturation of PSI electron acceptors, ultimately leading to the production of reactive oxygen species and photoinhibition of PSI. By adjusting the time of the light treatment, PSI can be gradually photoinhibited, providing a novel tool to holistically investigate the PSI photoinhibition phenomenon.

Pinaceae are the predominant photosynthetic species in boreal forests, but so far no detailed description of the protein components of the photosynthetic apparatus of these gymnosperms has been available. In this study we report a detailed characterization of the thylakoid photosynthetic machinery of Norway spruce (Picea abies (L.) Karst). We first customized a spruce thylakoid protein database from translated transcript sequences combined with existing protein sequences derived from gene models, which enabled reliable tandem mass spectrometry identification of P. abies thylakoid proteins from two-dimensional large pore blue-native/SDS-PAGE. This allowed a direct comparison of the two-dimensional protein map of thylakoid protein complexes from P. abies with the model angiosperm Arabidopsis thaliana. Although the subunit composition of P. abies core PSI and PSII complexes is largely similar to that of Arabidopsis, there was a high abundance of a smaller PSI subcomplex, closely resembling the assembly intermediate PSI* complex. In addition, the evolutionary distribution of light-harvesting complex (LHC) family members of Pinaceae was compared in silico with other land plants, revealing that P. abies and other Pinaceae (also Gnetaceae and Welwitschiaceae) have lost LHCB4, but retained LHCB8 (formerly called LHCB4.3). The findings reported here show the composition of the photosynthetic apparatus of P. abies and other Pinaceae members to be unique among land plants., The photosynthetic apparatus of Picea abies and other members of Pinaceae has a unique composition among land plants, demonstrated by a loss of individual LHC proteins and high amounts of PSI* complexes.