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NASA's Perseverance and ESA's Rosalind Franklin rovers have the scientific goal of searching for evidence of ancient life on Mars. Geochemical biosignatures that form because of microbe-mineral interactions could play a key role in achieving this, as they can be preserved for millions of years on Earth, and the same could be true for Mars. Previous laboratory experiments have explored the formation of biosignatures under closed systems, but these do not represent the open systems that are found in natural martian environments, such as channels and lakes. In this study, we have conducted environmental simulation experiments using a global regolith simulant (OUCM-1), a thermochemically modelled groundwater, and an anaerobic microbial community to explore the formation of geochemical biosignatures within plausible open and closed systems on Mars. This initial investigation showed differences in the diversity of the microbial community developed after 28 days. In an open-system simulation (flow-through experiment), the acetogenic Acetobacterium (49% relative abundance) and the sulfate reducer Desulfosporomusa (43% relative abundance) were the dominant genera. Whereas in the batch experiment, the sulfate reducers Desulfovibrio, Desulfomicrobium, and Desulfuromonas (95% relative abundance in total) were dominant. We also found evidence of enhanced mineral dissolution within the flow-through experiment, but there was little evidence of secondary deposits in the presence of biota. In contrast, SiO2 and Fe deposits formed within the batch experiment with biota but not under abiotic conditions. The results from these initial experiments indicate that different geochemical biosignatures can be generated between open and closed systems, and therefore, biosignature formation in open systems warrants further investigation.

Here, we report the draft genome sequences of strains of Bacillus and Salarachaeum that were isolated from hypersaline water samples collected from Lake Karum, Danakil Depression, Ethiopia. The sequences pave the way for more targeted studies into the potential biological activities and secondary metabolite synthesis of these organisms.

The waters that were present on early Mars may have been habitable. Characterising environments analogous to these waters and investigating the viability of their microbes under simulated martian chemical conditions is key to developing hypotheses on this habitability and potential biosignature formation. In this study, we examined the viability of microbes from the Anderton Brine Springs (United Kingdom) under simulated martian chemistries designed to simulate the chemical conditions of water that may have existed during the Hesperian. Associated changes in the fluid chemistries were also tested using inductively coupled plasma-optical emission spectroscopy (ICP-OES). The tested Hesperian fluid chemistries were shown to be habitable, supporting the growth of all of the Anderton Brine Spring isolates. However, inter and intra-generic variation was observed both in the ability of the isolates to tolerate more concentrated fluids and in their impact on the fluid chemistry. Therefore, whilst this study shows microbes from fluctuating brines can survive and grow in simulated martian water chemistry, further investigations are required to further define the potential habitability under past martian conditions.

Water present on the surface of early Mars (>3.0 Ga) may have been habitable. Characterising analogue environments and investigating the aspects of their microbiome best suited for growth under simulated martian chemical conditions is key to understanding potential habitability. Experiments were conducted to investigate the viability of microbes from a Mars analogue environment, Colour Peak Springs (Axel Heiberg Island, Canadian High Arctic), under simulated martian chemistries. The fluid was designed to emulate waters thought to be typical of the late Noachian, in combination with regolith simulant material based on two distinct martian geologies. These experiments were performed with a microbial community from Colour Peak Springs sediment. The impact on the microbes was assessed by cell counting and 16S rRNA gene amplicon sequencing. Changes in fluid chemistries were tested using ICP-OES. Both chemistries were shown to be habitable, with growth in both chemistries. Microbial communities exhibited distinct growth dynamics and taxonomic composition, comprised of sulfur-cycling bacteria, represented by either sulfate-reducing or sulfur-oxidising bacteria, and additional heterotrophic halophiles. Our data support the identification of Colour Peak Springs as an analogue for former martian environments, with a specific subsection of the biota able to survive under more accurate proxies for martian chemistries.

Introduction: The surface of Mars cannot sus-tain liquid water today, but there is evidence for the extended presence of liquid water during the Noa-chian era [1-2]. The transition of the martian cli-mate from the wet Noachian to the dry, late Hespe-rian would have resulted in saline and sulfur-rich surface waters [1-4]. Terrestrial analogue environ-ments that possess a similar chemistry to these pro-posed waters can be used to develop an understand-ing of the diversity of organisms that could have persisted under such conditions. Combining this with laboratory simulation experiments, which ena-ble a greater level of accuracy regarding the chemi-cal environment, allows for concepts regarding di-versity and function to be developed. Here we present the chemistry and microbial community of the highly reducing sediment of the springs of Colour Peak, a sulfidic and saline spring system located within the Canadian High Arctic [2]. We also present details of the viability of this mi-crobial community when grown in defined, simulat-ed martian fluid chemistries based on the chemistry of Rocknest at Gale crater in combination with ba-saltic and iron enriched martian simulants. Methodology: In this study, the elemental com-position of the fluids and sediment porewater of Colour Peak was determined by ICP-OES. This data was compared with a range of fluid chemistries, including those from other analogue environments and martian brines, the composition of which were determined based on the chemistry of the “Rock-nest” sand sample at Yellowknife Bay, Gale crater (Mars) by thermochemical modelling [5]. The fluid chemistry derived from the thermochemical model-ling was used to calculate Gibbs energy values to identify metabolic pathways that could be energeti-cally feasible. Molecular techniques were also used to investigate the microbial community of the sedi-ment of the Colour Peak Springs. Both DNA and RNA were extracted from the microbes in t

The martian surface cannot sustain liquid water today, but there is evidence water was present during the Noachian era. The transition of the martian climate into the Hesperian would have resulted in saline and sulfuric waters. Terrestrial analogue environments that possess a chemistry like these proposed waters can be used to develop an understanding of organisms that could have persisted. Here we present the chemistry and microbiome of Colour Peak, a sulfidic and saline spring system located within the Canadian High Arctic. Nucleic acids were extracted from the microbes in the sediments and the microbiome was characterised by the amplification and sequencing of 16S rRNA gene amplicons. The elemental composition of the fluids and sediment was determined by ICP-OES and compared with brines determined from the chemistry of the “Rocknest” sample at Yellowknife Bay, Gale Crater (Mars) by thermochemical modelling. Gibbs energy values were calculated from this fluid chemistry to identify potentially viable metabolisms. Analysis of the chemistries of the Colour Peak fluids confirmed a composition like the thermochemically modelled fluid, providing justification for the classification of Colour Peak as an appropriate analogue environment to investigate the habitability of former martian waters. Profiling of the Colour Peak microbial community revealed domination by bacteria associated with oxidation of reduced sulfur species and carbon dioxide fixation. Gibbs energy values calculated using the modelled martian fluid chemistry demonstrated that oxidation of reduced sulfur species was also viable in this chemical environment under aerobic and anaerobic conditions. These results demonstrate microbial sulfide oxidation is thermodynamically viable using both modelled and environmental proxies for former martian aqueous environments. This study highlights that metabolisms utilising the oxidation of reduced sulfur species cou

The surface of Mars cannot sustain liquid water today, but there is evidence water was present during the Noachian era. The transition of the martian climate from the wet Noachian to the dry Hesperian would have resulted in saline and sulfur rich surface waters . Terrestrial analogue environments that possess a chemistry like these proposed waters can be used to develop an understanding of organisms that could have persisted under such conditions. Here we present the chemistry and microbiome of the analogue environment Colour Peak, a sulfidic and saline spring system located within the Canadian High Arctic. In this study, molecular and geochemical techniques were used to investigate the sediment of the Colour Peak springs. Nucleic acids were extracted from the microbes in the sediments and the microbiome was characterised by the amplification and sequencing of 16S rRNA gene amplicons. The elemental composition of the fluids and sediment was determined by ICP-OES and compared with brines determined from the chemistry of the “Rocknest” sand sample at Yellowknife Bay, Gale Crater (Mars) by thermochemical modelling. Gibbs energy values were calculated from this fluid chemistry to identify potentially viable metabolisms. Analysis of the chemistries of the Colour Peak fluids confirmed a chemical composition like the thermochemically modelled fluid, with this justifying the classification of Colour Peak as an appropriate analogue environment to investigate the habitability of former martian aqueous environments. 16S rRNA gene profiling of the Colour Peak microbial community revealed it was dominated by bacteria associated with oxidation of reduced sulfur species and carbon dioxide fixation. Gibbs energy values calculated using the chemistry of the modelled martian fluid demonstrated that the oxidation of reduced sulfur species was also viable in this chemical environment under aerobic and anaerobic conditions. These results demonstrate that microbial sulfide oxidation is t

Water present on early Mars is often assumed to have been habitable. In this study, experiments were performed to investigate the habitability of well-defined putative martian fluids and to identify the accompanying potential formation of biosignatures. Simulated martian environments were developed by combining martian fluid and regolith simulants based on the chemistry of the Rocknest sand shadow at Gale Crater. The simulated chemical environment was inoculated with terrestrial anoxic sediment from the Pyefleet mudflats (United Kingdom). These enrichments were cultured for 28 days and subsequently subcultured seven times to ensure that the microbial community was solely grown on the defined, simulated chemistry. The impact of the simulated chemistries on the microbial community was assessed by cell counts and sequencing of 16S rRNA gene profiles. Associated changes to the fluid and precipitate chemistries were established by using ICP-OES, IC, FTIR, and NIR. The fluids were confirmed as habitable, with the enriched microbial community showing a reduction in abundance and diversity over multiple subcultures relating to the selection of specific metabolic groups. The final community comprised sulfate-reducing, acetogenic, and other anaerobic and fermentative bacteria. Geochemical characterization and modeling of the simulant and fluid chemistries identified clear differences between the biotic and abiotic experiments. These differences included the elimination of sulfur owing to the presence of sulfate-reducing bacteria and more general changes in pH associated with actively respiring cells that impacted the mineral assemblages formed. This study confirmed that a system simulating the fluid chemistry of Gale Crater could support a microbial community and that variation in chemistries under biotic and abiotic conditions can be used to inform future life-detection missions.

Introduction: The surface of Mars cannot sus-tain liquid water today, but there is evidence for the extended presence of liquid water during the Noa-chian era [1-2]. The transition of the martian cli-mate from the wet Noachian to the dry, late Hespe-rian would have resulted in saline and sulfur-rich surface waters [1-4]. Terrestrial analogue environ-ments that possess a similar chemistry to these pro-posed waters can be used to develop an understand-ing of the diversity of organisms that could have persisted under such conditions. Combining this with laboratory simulation experiments, which ena-ble a greater level of accuracy regarding the chemi-cal environment, allows for concepts regarding di-versity and function to be developed. Here we present the chemistry and microbial community of the highly reducing sediment of the springs of Colour Peak, a sulfidic and saline spring system located within the Canadian High Arctic [2]. We also present details of the viability of this mi-crobial community when grown in defined, simulat-ed martian fluid chemistries based on the chemistry of Rocknest at Gale crater in combination with ba-saltic and iron enriched martian simulants. Methodology: In this study, the elemental com-position of the fluids and sediment porewater of Colour Peak was determined by ICP-OES. This data was compared with a range of fluid chemistries, including those from other analogue environments and martian brines, the composition of which were determined based on the chemistry of the “Rock-nest” sand sample at Yellowknife Bay, Gale crater (Mars) by thermochemical modelling [5]. The fluid chemistry derived from the thermochemical model-ling was used to calculate Gibbs energy values to identify metabolic pathways that could be energeti-cally feasible. Molecular techniques were also used to investigate the microbial community of the sedi-ment of the Colour Peak Springs. Both DNA and RNA were extracted from the microbes in t

The waters that were present on early Mars may have been habitable. Characterising environments analogous to these waters and investigating the viability of their microbes under simulated martian chemical conditions is key to developing hypotheses on this habitability and potential biosignature formation. In this study, we examined the viability of microbes from the Anderton Brine Springs (United Kingdom) under simulated martian chemistries designed to simulate the chemical conditions of water that may have existed during the Hesperian. Associated changes in the fluid chemistries were also tested using inductively coupled plasma-optical emission spectroscopy (ICP-OES). The tested Hesperian fluid chemistries were shown to be habitable, supporting the growth of all of the Anderton Brine Spring isolates. However, inter and intra-generic variation was observed both in the ability of the isolates to tolerate more concentrated fluids and in their impact on the fluid chemistry. Therefore, whilst this study shows microbes from fluctuating brines can survive and grow in simulated martian water chemistry, further investigations are required to further define the potential habitability under past martian conditions.

Water present on the surface of early Mars (>3.0 Ga) may have been habitable. Characterising analogue environments and investigating the aspects of their microbiome best suited for growth under simulated martian chemical conditions is key to understanding potential habitability. Experiments were conducted to investigate the viability of microbes from a Mars analogue environment, Colour Peak Springs (Axel Heiberg Island, Canadian High Arctic), under simulated martian chemistries. The fluid was designed to emulate waters thought to be typical of the late Noachian, in combination with regolith simulant material based on two distinct martian geologies. These experiments were performed with a microbial community from Colour Peak Springs sediment. The impact on the microbes was assessed by cell counting and 16S rRNA gene amplicon sequencing. Changes in fluid chemistries were tested using ICP-OES. Both chemistries were shown to be habitable, with growth in both chemistries. Microbial communities exhibited distinct growth dynamics and taxonomic composition, comprised of sulfur-cycling bacteria, represented by either sulfate-reducing or sulfur-oxidising bacteria, and additional heterotrophic halophiles. Our data support the identification of Colour Peak Springs as an analogue for former martian environments, with a specific subsection of the biota able to survive under more accurate proxies for martian chemistries.

The martian surface cannot sustain liquid water today, but there is evidence water was present during the Noachian era. The transition of the martian climate into the Hesperian would have resulted in saline and sulfuric waters. Terrestrial analogue environments that possess a chemistry like these proposed waters can be used to develop an understanding of organisms that could have persisted. Here we present the chemistry and microbiome of Colour Peak, a sulfidic and saline spring system located within the Canadian High Arctic. Nucleic acids were extracted from the microbes in the sediments and the microbiome was characterised by the amplification and sequencing of 16S rRNA gene amplicons. The elemental composition of the fluids and sediment was determined by ICP-OES and compared with brines determined from the chemistry of the “Rocknest” sample at Yellowknife Bay, Gale Crater (Mars) by thermochemical modelling. Gibbs energy values were calculated from this fluid chemistry to identify potentially viable metabolisms. Analysis of the chemistries of the Colour Peak fluids confirmed a composition like the thermochemically modelled fluid, providing justification for the classification of Colour Peak as an appropriate analogue environment to investigate the habitability of former martian waters. Profiling of the Colour Peak microbial community revealed domination by bacteria associated with oxidation of reduced sulfur species and carbon dioxide fixation. Gibbs energy values calculated using the modelled martian fluid chemistry demonstrated that oxidation of reduced sulfur species was also viable in this chemical environment under aerobic and anaerobic conditions. These results demonstrate microbial sulfide oxidation is thermodynamically viable using both modelled and environmental proxies for former martian aqueous environments. This study highlights that metabolisms utilising the oxidation of reduced sulfur species cou

The surface of Mars cannot sustain liquid water today, but there is evidence water was present during the Noachian era. The transition of the martian climate from the wet Noachian to the dry Hesperian would have resulted in saline and sulfur rich surface waters . Terrestrial analogue environments that possess a chemistry like these proposed waters can be used to develop an understanding of organisms that could have persisted under such conditions. Here we present the chemistry and microbiome of the analogue environment Colour Peak, a sulfidic and saline spring system located within the Canadian High Arctic. In this study, molecular and geochemical techniques were used to investigate the sediment of the Colour Peak springs. Nucleic acids were extracted from the microbes in the sediments and the microbiome was characterised by the amplification and sequencing of 16S rRNA gene amplicons. The elemental composition of the fluids and sediment was determined by ICP-OES and compared with brines determined from the chemistry of the “Rocknest” sand sample at Yellowknife Bay, Gale Crater (Mars) by thermochemical modelling. Gibbs energy values were calculated from this fluid chemistry to identify potentially viable metabolisms. Analysis of the chemistries of the Colour Peak fluids confirmed a chemical composition like the thermochemically modelled fluid, with this justifying the classification of Colour Peak as an appropriate analogue environment to investigate the habitability of former martian aqueous environments. 16S rRNA gene profiling of the Colour Peak microbial community revealed it was dominated by bacteria associated with oxidation of reduced sulfur species and carbon dioxide fixation. Gibbs energy values calculated using the chemistry of the modelled martian fluid demonstrated that the oxidation of reduced sulfur species was also viable in this chemical environment under aerobic and anaerobic conditions. These results demonstrate that microbial sulfide oxidation is t

The identification of novel terrestrial sites that are analogous for other planetary bodies is an active area of research within astrobiology, because of the logistical and financial difficulties in obtaining extraterrestrial samples for analysis. Characterisation of potential analogue sites is undertaken to assess how accurately they represent a specific extraterrestrial environment. Analysing their physicochemical conditions and microbial communities are key components of these studies to understand what metabolisms would be viable in such environments. One such novel analogue environment is the salt plains of Western Sahara. Western Sahara is one of the driest regions on Earth. It is located on the northwest coast of West Africa and is characterised by high UV exposure, low annual precipitation and water activity, subsurface water and high annual temperatures. These features make Western Sahara a potential analogue site for Mars during the Noachian-Hesperian transition period (3.5 – 3.8 Ga), when the atmosphere began to thin and surface water started evaporating (Warner et al., 2010), similar to other terrestrial deserts, such as the Atacama Desert and the McMurdo Dry Valleys. The hypersalinity, aridity and high UV radiation levels of the Western Sahara salt plains would also be appropriate to study whether dissimilatory sulfur metabolisms would be viable in a Noachian-Hesperian Mars analogue environment. Dissimilatory sulfur cycling refers to the use of inorganic sulfur compounds for energy conservation and it has been recognised as a metabolic strategy of interest for putative martian life (Macey et al., 2020). On Earth, evidence from stable sulfur isotope fractionation has suggested this metabolism emerged early in the history of life (~3.5 Ga). During this period, the conditions on Mars were predicted as being more habitable than present-day, with an active magnetic field, thicker atmosphere and liquid water on the surf

The Cassini Mission confirmed Enceladus, a moon of Saturn, has a sub-surface ocean that is hydrothermally active and contains the ingredients for life. The moon also produces supersonic plumes that eject aerosolised droplets of ocean water into space, likely formed by vigorous bubbling of hydrothermal gases. Dependent on these bubbling mechanics, if life is present in the ocean, long distance microbial dispersion may occur via the plumes allowing for sample by spacecraft. Despite this theory, it is unknown what evidence of microbial life might be transferred into the plumes by this bubbling. This presentation discusses preliminary results of a field campaign that used Iceland’s geothermal springs as analogue sites for Enceladus plume formation. Iceland was selected as its geothermal springs and Enceladus’s ocean share aerosolization driven by bubbling of hydrothermal gases and both host niches for chemotrophic microbial communities. In situ sampling and aerosol monitoring was undertaken at Ölkelduháls, within the Hengill volcanic massif, at bubbling hot springs of varying temperatures (65oC – 86oC), pH (2.5 – 6.5) and size. Total aerosol particle count decreased with downwind distance from the springs. Despite this, particle size distribution was dominated by larger particles (≤10 µm in diameter) at all distances from the aerosol source. This observation is likely due to present bubbling mechanisms, as background samples contained proportionally fewer particles ≤ 10 µm in diameter. Future analysis will quantify aerosol microbial abundance and size distributions using Flow Cytometry. Microbial community structure in the aerosol will also be investigated using DNA sequence analysis techniques. Combined, these analyses will provide detailed information regarding whether the captured aerosol microbes represent the entire spring’s community, or just a snapshot. Overall, this work can make predictions about microbial dispersion dynamics in the plumes of Enceladus if life