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Pharmaceuticals and ionic liquids (ILs) are emerging as significant micropollutants with environmental presence and potential ecological impacts. The possible simultaneous occurrence of these two groups of pollutants in aquatic environments raises complex challenges due to their diverse chemical properties and potential for interactive effects. Given the documented widespread presence of pharmaceuticals and the emerging concerns about ILs, the study aims to evaluate the adverse effects of binary mixtures of imidazolium ionic liquid IM1-8C(CN) 3 and two representatives of pharmaceuticals: antibiotic oxytetracycline (OXTC) and metabolite carbamazepine 10,11 epoxide (CBZ-E) on the brackish cyanobacterium Microcystis aeruginosa and the marine diatom Phaeodactylum tricornutum during chronic exposure experiments. A comprehensive approach was employed, incorporating various endpoints including oxidative stress, chlorophyll a fluorescence, detailed photoprotective and photosynthetic pigment profiles of target microorganisms to assess modes of action and identify the mixture effects of the selected substances. The observed alterations in pigment production affecting carotenoids synthesis in both selected species may be attributed to the differential impacts of these substances on the photosynthetic pathways and metabolic processes in the cyanobacterial and diatom cells. Changes in chlorophyll a fluorescence-specific parameters suggest impairment of the photosynthetic activity, particularly affecting the efficiency of photosystem II. The application of Concentration Addition (CA) and Independent Action (IA) mathematical models, complemented by the evaluation of Model Deviation Ratios (MDR), revealed predominantly antagonistic interactions within the studied mixtures. The findings of this study provide important insights into the effects of mixtures of organic micropollutants and their potential impact on environment including brackish and marine waters., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Lilianna Sharma reports financial support was provided by Institute of Oceanology Polish Academy of Sciences. Lilianna Sharma reports a relationship with National Science Centre Poland that includes: funding grants. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

The classic melatonin biosynthesis pathway (Mel; N-acetyl-5-methoxytryptamine) involves two consecutive enzymatic steps that are decisive in hormone production: conversion of serotonin (5-hydroxytryptamine; 5-HT) to N-acetylserotonin (NAS) and the methylation of the last compound to Mel. This pathway requires the activity of the enzymes: the first is of the category of N-acetyltransferases (AANAT, SNAT, or NAT) and the second is N-acetylserotonin O-methyltransferase (ASMT; also known as HIOMT). However, quite recently, new information has been provided on the possibility of an alternative Mel synthesis pathway; it would include a two-step action by these enzymes, but in reverse order, where ASMT (or ASMTL, the enzyme related to ASMT) methylates 5-HT to 5-methoxytryptamine (5-MT), and then the last compound is acetylated by an enzyme of the category of N-acetyltransferases to Mel. In our study on the activity of enzymes in the Mel biosynthesis pathway in flounder skin, we have found an increase in 5-MT level, as a result of the increase in 5-HT concentration, which is followed by a growing concentration of Mel. However, we have not found any increase in Mel concentration, despite an increase in NAS in the samples. Our data strongly suggest an alternative way of Mel production in flounder skin in which 5-HT is first methylated to 5-MT, which is then acetylated to Mel., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

This comprehensive study examines primary production (PP) within the Spitsbergen fjords, Hornsund, and Kongsfjord, over a 25-year period (1994-2019), across 45 stations and 348 incubation levels at various depths. PP and hydrological parameters were measured at 28 sampling stations in Kongsfjorden and 17 in Hornsund, with the locations of "Glacier," "Inner," and "Outer" zones defined to reflect the varying influence of glacial meltwater. Our study revealed spatial and temporal variability in PP, both at the surface and within the water column with very high depth resolution. The highest PP values were observed in the Glacier and Inner zones of Hornsund, particularly in the water layer up to 3 m depth, exceeding 20 mgC m -3  h -1 . A notable decline in PP with increasing depth was observed in both fjords, with the Glacier zones displaying the highest productivity at the surface. The study also highlights the influence of glacial meltwater on surface water conditions, affecting the PP in the upper layers of both fjords. The observed gradient in the depth of maximum PP toward the mouth of the fjord varied between the two fjords, with Kongsjord displaying more dynamic variations. The spatial distribution of integrated primary production (Pi) suggested lower productivity in the glacial regions, likely due to light limitation caused by high concentrations of mineral particulate matter. The values of Pi were considerably higher in Hornsund, approximately twice as high overall, with specific emphasis on the Glacier and Inner zones where Pi values were about 6.5 and 2.5 times higher, respectively, when compared to those observed in Kongsfjord., Competing Interests: The authors declare that they have no conflict of interest., (© 2024 The Author(s). Ecology and Evolution published by John Wiley & Sons Ltd.)

Copepods of the genus Calanus are the key components of zooplankton. Understanding their response to a changing climate is crucial to predict the functioning of future warmer high-latitude ecosystems. Although specific Calanus species are morphologically very similar, they have different life strategies and roles in ecosystems. In this study, C. finmarchicus and C. glacialis were thoroughly studied with regard to their plasticity in morphology and ecology both in their preferred original water mass (Atlantic vs. Arctic side of the Polar Front) and in suboptimal conditions (due to, e.g., temperature, turbidity, and competition in Hornsund fjord). Our observations show that "at the same place and time," both species can reach different sizes, take on different pigmentation, be in different states of population development, utilize different reproductive versus lipid accumulation strategies, and thrive on different foods. Size was proven to be a very mutable morphological trait, especially with regard to reduced length of C. glacialis . Both species exhibited pronounced red pigmentation when inhabiting their preferred water mass. In other domains, C. finmarchicus individuals tended to be paler than C. glacialis individuals. Gonad maturation and population development indicated mixed reproductive strategies, although a surprisingly similar population age structure of the two co-occurring species in the fjord was observed. Lipid accumulation was high and not species-specific, and its variability was due to diet differences of the populations. According to the stable isotope composition, both species had a more herbivorous diatom-based diet in their original water masses. While the diet of C. glacialis was rather consistent among the domains studied, C. finmarchicus exhibited much higher variability in its feeding history (based on lipid composition). Our results show that the plasticity of both Calanus species is indeed impressive and may be regulated differently, depending on whether they live in their "comfort zone" or beyond it., Competing Interests: None declared., (© 2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.)

The optical properties, i.e., absorption and scattering spectra of ten strains of cyanobacteria from the Baltic Sea and Pomeranian lakes ( Aphanizomenon flos-aquae KAC 15, Microcystis aeruginosa CCNP 1101, Anabaena sp. CCNP 1406, Synechocystis salina CCNP 1104, Phormidium sp. CCNP 1317, Nodularia spumigena CCNP 1401, Synechococcus sp. CCNP 1108, Nostoc sp. CCNP 1411, Cyanobacterium sp. CCNP 1105, Pseudanabaena cf. galeata CCNP 1312) grown under low light conditions were investigated. Moreover, the chlorophylls, carotenoids, and phycobilin composition as well as the size structure of chosen cyanobacteria were measured. Studied species revealed high diversity both in optical properties with the absorption spectra similarity index ranging from 0.67 to 0.94 and the pigment composition. The chlorophyll-specific absorption coefficient at 440 nm a ph *(440) varied between 0.017 and 0.065 m 2  mg -1 . The influence of the package effect was only observed in the case of large filamentous cyanobacteria like N. spumigena or Nostoc sp. Interestingly, the package effect factor Q a *(675) for large-celled Anabaena sp. was 0.92. Besides chlorophyll a , only echinenone, β -carotene, and phycocyanin were present in all analyzed cyanobacteria strains. Zeaxanthin, which is widely used as a marker pigment for cyanobacteria, was absent in the toxic N. spumigena and Anabaena sp., which are the species that occur in the Baltic Sea most frequently causing summer cyanobacterial blooms. The investigation also showed that the sample preservation technique can introduce some major errors within the absorption band affected by the phycocyanin absorption.

Phycobilins are an important group of pigments that through complementary chromatic adaptation optimize the light-harvesting process in phytoplankton cells, exhibiting great potential as cyanobacteria species biomarkers. In their extracted form, concentrations of these water-soluble molecules are not easily determined using the chromatographic methods well suited to solvent-soluble pigments. Insights regarding the quantitative spectroscopic analysis of extracted phycobilins also remain limited. Here, we present an in-depth study of two methods that utilize the spectral properties of phycobilins in aqueous extracts. The technical work was carried out using high-purity standards of phycocyanin, phycoerythrin, and allophycocyanin. Calibration parameters for the spectrofluorometer and spectrophotometer were established. This analysis indicated the possibility of detecting pigments in concentrations ranging from 0.001 to 10 μg cm -3 . Fluorescence data revealed a reproducibility of 95 %. The differences in detection limits between the two methods enable the presence of phycobilins to be investigated and their amounts to be monitored from oligotrophic to eutrophic aquatic environments.

Aerobic anoxygenic phototrophs were recently found to constitute a significant portion of the marine microbial community. These bacteria use bacteriochlorophyll-containing reaction centers to perform photoheterotrophic metabolism. A new instrument for routine measurements of both chlorophyll a and bacteriochlorophyll a was used for monitoring anoxygenic phototrophs in the Baltic Sea in late summer 2003. Bacteriochlorophyll a concentration ranged from 8 to 50 ngl(-1), with an average bacteriochlorophyll/chlorophyll ratio of 4.2 x 10(-3). Moreover, diel trends in bacteriochlorophyll a signals were observed, with a distinct decline occurring during daylight hours. Based on laboratory measurements this phenomenon was ascribed to the complete inhibition of bacteriochlorophyll synthesis by light, which, in combination with a concurrent turnover of the cells, resulted in a pigment decline. Following this explanation, we postulate that bacteriochlorophyll a can serve as a natural 'pulse-and-chase' marker, allowing estimation of the mortality rates of anoxygenic phototrophs from the rates of pigment decline. Based on this assumption, we suggest that the Baltic photoheterotrophic community was characterized by high turnover rates, in a range of 0.7-2 d(-1).