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16 results on '"Stojko, Johann"'

7. TCTP contains a BH3-like domain, which instead of inhibiting, activates Bcl-xL

Author

Thébault, Stéphanie, Agez, Morgane, Chi, Xiaoke, Stojko, Johann, Cura, Vincent, Telerman, Stéphanie B., Maillet, Laurent, Gautier, Fabien, Billas-Massobrio, Isabelle, Birck, Catherine, Troffer-Charlier, Nathalie, Karafin, Teele, Honoré, Joane, Senff-Ribeiro, Andrea, Montessuit, Sylvie, Johnson, Christopher M., Juin, Philippe, Cianférani, Sarah, Martinou, Jean-Claude, Andrews, David W., Amson, Robert, Telerman, Adam, and Cavarelli, Jean

Published
2016
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8. Stereotactic biopsies of brainstem lesions: dilemma on the best trajectory

Author

Chabrol, Eric, Fagnen, Charline, Landron, Sophie, Marcheteau, Estelle, Stojko, Johann, Guenin, Sophie‐Pénélope, Antoine, Mathias, Fould, Benjamin, Ferry, Gilles, Boutin, Jean, Vénien‐Bryan, Catherine, Institut de Recherches SERVIER (IRS), Institut de minéralogie, de physique des matériaux et de cosmochimie (IMPMC), Muséum national d'Histoire naturelle (MNHN)-Institut de recherche pour le développement [IRD] : UR206-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Institut de Recherches Internationales Servier [Suresnes] (IRIS)

Subjects
[SDU.STU.MI]Sciences of the Universe [physics]/Earth Sciences/Mineralogy
Abstract

International audience; VHH stands for the variable regions of heavy chain only of camelid IgGs. The VHH family forms a set of interesting proteins derived from antibodies that maintain their capacity to recognize the antigen, despite their relatively small molecular weight (in the 12,000 Da range). Continuing our exploration of the possibilities of those molecules, we chose to design alternative molecules with maintained antigen recognition, but enhanced capacity, by fusing four VHH with one Fc, the fragment crystallizable region of antibodies. In doing so, we aimed at having a molecule with superior quantitative antigen recognition (×4) while maintaining its size below the 110 kDa. In the present paper, we described the building of those molecules that we coined VHH2-Fc-VHH2. The structure of VHH2-Fc-VHH2 in complex with HER2 antigen was determined using electronic microscopy and modeling. The molecule is shown to bind four HER2 proteins at the end of its flexible arms. VHH2-Fc-VHH2 also shows an internalization capacity via HER2 receptor superior to the reference anti-HER2 monoclonal antibody, Herceptin®, and to a simple fusion of two VHH with one Fc (VHH2-Fc). This new type of molecules, VHH2-Fc-VHH2, could be an interesting addition to the therapeutic arsenal with multiple applications, from diagnostic to therapy.

Published
2021
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10. Redox regulation : insights into the interaction mechanism of sulfiredoxin with peroxiredoxin and ATP

Author

Florent, Canonico, Beaussart, Audrey, Stojko, Johann, Kriznik, Alexandre, Cianférani, Sarah, Mazon, Hortense, Rahuel-Clermont, Sophie, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Spectrométrie de Masse BioOrganique [Strasbourg] (LSMBO), Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), and RAHUEL-CLERMONT, Sophie

Subjects
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

11. VHH characterization. Comparison of recombinant with chemically synthesized anti‐HER2 VHH

Author

Hartmann, Lucie, primary, Botzanowski, Thomas, additional, Galibert, Mathieu, additional, Jullian, Magali, additional, Chabrol, Eric, additional, Zeder‐Lutz, Gabrielle, additional, Kugler, Valérie, additional, Stojko, Johann, additional, Strub, Jean‐Marc, additional, Ferry, Gilles, additional, Frankiewicz, Lukasz, additional, Puget, Karine, additional, Wagner, Renaud, additional, Cianférani, Sarah, additional, and Boutin, Jean A., additional

Published
2019
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12. S29434, a Quinone Reductase 2 Inhibitor: Main Biochemical and Cellular Characterization

Author

Boutin, Jean A., primary, Bouillaud, Frederic, additional, Janda, Elzbieta, additional, Gacsalyi, István, additional, Guillaumet, Gérald, additional, Hirsch, Etienne C., additional, Kane, Daniel A., additional, Nepveu, Françoise, additional, Reybier, Karine, additional, Dupuis, Philippe, additional, Bertrand, Marc, additional, Chhour, Monivan, additional, Le Diguarher, Thierry, additional, Antoine, Mathias, additional, Brebner, Karen, additional, Da Costa, Hervé, additional, Ducrot, Pierre, additional, Giganti, Adeline, additional, Goswami, Vishalgiri, additional, Guedouari, Hala, additional, Michel, Patrick P., additional, Patel, Aakash, additional, Paysant, Jérôme, additional, Stojko, Johann, additional, Viaud-Massuard, Marie-Claude, additional, and Ferry, Gilles, additional

Published
2018
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13. Soaking suggests “alternative facts”: Only co-crystallization discloses major ligand-induced interface rearrangements of a homodimeric tRNA-binding protein indicating a novel mode-of-inhibition

Author

Ehrmann, Frederik Rainer, primary, Stojko, Johann, additional, Metz, Alexander, additional, Debaene, François, additional, Barandun, Luzi Jakob, additional, Heine, Andreas, additional, Diederich, François, additional, Cianférani, Sarah, additional, Reuter, Klaus, additional, and Klebe, Gerhard, additional

Published
2017
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16. S29434, a Quinone Reductase 2 Inhibitor: Main Biochemical and Cellular Characterization

Author

Boutin, Jean A., Bouillaud, Frederic, Janda, Elzbieta, Gacsalyi, István, Guillaumet, Gérald, Hirsch, Etienne C., Kane, Daniel A., Nepveu, Francoise, Reybier, Karine, Dupuis, Philippe, Bertrand, Marc, Chhour, Monivan, Le Diguarher, Thierry, Antoine, Mathias, Brebner, Karen, Da Costa, Hervé, Ducrot, Pierre, Giganti, Adeline, Goswami, Vishalgiri, Guedouari, Hala, Michel, Patrick P., Patel, Aakash, Paysant, Jérôme, Stojko, Johann, Viaud-Massuard, Marie-Claude, and Ferry, Gilles

Abstract

Quinone reductase 2 (QR2, E.C. 1.10.5.1) is an enzyme with a feature that has attracted attention for several decades: in standard conditions, instead of recognizing NAD(P)H as an electron donor, it recognizes putative metabolites of NADH, such as N-methyl- and N-ribosyl-dihydronicotinamide. QR2 has been particularly associated with reactive oxygen species and memory, strongly suggesting a link among QR2 (as a possible key element in pro-oxidation), autophagy, and neurodegeneration. In molecular and cellular pharmacology, understanding physiopathological associations can be difficult because of a lack of specific and powerful tools. Here, we present a thorough description of the potent, nanomolar inhibitor [2-(2-methoxy-5H-1,4b,9-triaza(indeno[2,1-a]inden-10-yl)ethyl]-2-furamide (S29434 or NMDPEF; IC50= 5–16 nM) of QR2 at different organizational levels. We provide full detailed syntheses, describe its cocrystallization with and behavior at QR2 on a millisecond timeline, show that it penetrates cell membranes and inhibits QR2-mediated reactive oxygen species (ROS) production within the 100 nM range, and describe its actions in several in vivo models and lack of actions in various ROS-producing systems. The inhibitor is fairly stable in vivo, penetrates cells, specifically inhibits QR2, and shows activities that suggest a key role for this enzyme in different pathologic conditions, including neurodegenerative diseases.

Published
2019
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