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Your search keyword '"Streppel, Mirte M"' showing total 7 results
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7 results on '"Streppel, Mirte M"'

4. Pancreatic Cancer and Immunotherapy: A Clinical Overview

Author

Timmer, Florentine E. F., primary, Geboers, Bart, additional, Nieuwenhuizen, Sanne, additional, Dijkstra, Madelon, additional, Schouten, Evelien A. C., additional, Puijk, Robbert S., additional, de Vries, Jan J. J., additional, van den Tol, M. Petrousjka, additional, Bruynzeel, Anna M. E., additional, Streppel, Mirte M., additional, Wilmink, Johanna W., additional, van der Vliet, Hans J., additional, Meijerink, Martijn R., additional, Scheffer, Hester J., additional, and de Gruijl, Tanja D., additional

Published
2021
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5. Exome sequencing identifies frequent inactivating mutations in BAP1, ARID1A and PBRM1 in intrahepatic cholangiocarcinomas

Author

Jiao, Yuchen, primary, Pawlik, Timothy M, additional, Anders, Robert A, additional, Selaru, Florin M, additional, Streppel, Mirte M, additional, Lucas, Donald J, additional, Niknafs, Noushin, additional, Guthrie, Violeta Beleva, additional, Maitra, Anirban, additional, Argani, Pedram, additional, Offerhaus, G Johan A, additional, Roa, Juan Carlos, additional, Roberts, Lewis R, additional, Gores, Gregory J, additional, Popescu, Irinel, additional, Alexandrescu, Sorin T, additional, Dima, Simona, additional, Fassan, Matteo, additional, Simbolo, Michele, additional, Mafficini, Andrea, additional, Capelli, Paola, additional, Lawlor, Rita T, additional, Ruzzenente, Andrea, additional, Guglielmi, Alfredo, additional, Tortora, Giampaolo, additional, de Braud, Filippo, additional, Scarpa, Aldo, additional, Jarnagin, William, additional, Klimstra, David, additional, Karchin, Rachel, additional, Velculescu, Victor E, additional, Hruban, Ralph H, additional, Vogelstein, Bert, additional, Kinzler, Kenneth W, additional, Papadopoulos, Nickolas, additional, and Wood, Laura D, additional

Published
2013
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7. Sialofucosylated podocalyxin is a functional E- and L-selectin ligand expressed by metastatic pancreatic cancer cells.

Author

Dallas MR, Chen SH, Streppel MM, Sharma S, Maitra A, and Konstantopoulos K

Subjects
Animals, CHO Cells, Cell Line, Tumor, Cricetinae, Cricetulus, Gene Knockdown Techniques, Humans, Ligands, Pancreatic Neoplasms secondary, Sialoglycoproteins metabolism, E-Selectin metabolism, Fucose metabolism, Gene Expression Regulation, Neoplastic, L-Selectin metabolism, N-Acetylneuraminic Acid metabolism, Pancreatic Neoplasms metabolism, Sialoglycoproteins physiology
Abstract

Selectin-mediated interactions in the vasculature promote metastatic spread by facilitating circulating tumor cell binding to selectin-expressing host cells. Therefore, identifying the selectin ligand(s) on tumor cells is critical to the prevention of blood-borne metastasis. A current challenge is to distinguish between structures expressed by circulating tumor cells that can bind selectins in vitro from the functional ligands whose depletion suppresses selectin-dependent binding under flow in vivo. Interestingly, podocalyxin (PODXL), which can bind E- and L-selectin, is upregulated in a number of cancers, including those of the breast, colon, and pancreas. In this work, we show that metastatic pancreatic cancer cells overexpress PODXL compared with nonmalignant pancreatic epithelial cells. We further demonstrate via tissue microarray that 69% of pancreatic ductal adenocarcinomas stain positive for PODXL. In cases of focal expression, positive staining is restricted to the invasive front of primary tumors. By combining immunoblot, immunodepletion, short-hairpin RNA-mediated gene silencing, and flow-based adhesion assays, we evaluated the functional role of sialofucosylated PODXL in selectin-mediated adhesion under flow. Our data indicate that sialofucosylated PODXL is a functional E- and L-selectin ligand expressed by metastatic pancreatic cancer cells, as specific depletion of this molecule from the cell surface significantly interferes with selectin-dependent interactions. Cumulatively, these data support a correlation between sialofucosylated PODXL expression and enhanced binding to selectins by metastatic pancreatic cancer cells and offer additional perspective on the upregulation of PODXL in aggressive cancers.

Published
2012
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