21 results on '"Su, Bing-Yin"'
Search Results
2. Morphological changes of cholinergic nerve fibers in the urinary bladder after establishment of artificial somatic-autonomic reflex arc in rats
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Wang, Han-Zhi / 王晗知, Li, Shu-Rong / 李淑蓉, Wen, Can / 文灿, Xiao, Chuan-Guo / 肖传国, and Su, Bing-Yin / 苏炳银
- Published
- 2007
- Full Text
- View/download PDF
3. Preconditioning with recombinant high-mobility group box 1 induces ischemic tolerance in a rat model of focal cerebral ischemia-reperfusion
- Author
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Xiao-Xi Liu, Yafang Hu, Jing-jing Wei, Chen Wang, Kaibin Huang, Yong Gu, Guo-Qing Zheng, and Su-bing Yin
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Male ,0301 basic medicine ,Ischemia ,Brain damage ,Pharmacology ,HMGB1 ,Biochemistry ,Neuroprotection ,Brain Ischemia ,Cell Line ,Brain ischemia ,Mice ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,medicine ,Animals ,HMGB1 Protein ,Rats, Wistar ,Ischemic Preconditioning ,biology ,business.industry ,medicine.disease ,Recombinant Proteins ,Rats ,030104 developmental biology ,Reperfusion Injury ,biology.protein ,TLR4 ,Ischemic preconditioning ,medicine.symptom ,business ,Neuroscience ,Reperfusion injury ,030217 neurology & neurosurgery - Abstract
Preconditioning with ligands of toll-like receptors (TLRs) is a powerful neuroprotective approach whereby a low dose of stimulus confers significant protection against subsequent substantial brain damage by reprogramming the ischemia-activated TLRs signaling. Herein, we aim to explore whether preconditioning with recombinant high-mobility group box 1 (rHMGB1), one of the TLRs ligands, decreases cerebral ischemia-reperfusion injury (IRI). Rats were intracerebroventricularly pretreated with rHMGB1, 1 or 3 days before induction of middle cerebral artery occlusion. Results showed that preconditioning with rHMGB1 1 day, but not 3 days, prior to ischemia dramatically reduced neurological deficits, infarct size, brain swelling, cell apoptosis, and blood-brain barrier permeability. Interleukin-1R-associated kinase-M (IRAK-M), a critical negative regulator of TLRs signaling, was robustly increased in response to brain IRI and was further elevated by rHMGB1 pretreatment, indicating its role associated with the rHMGB1 preconditioning-mediated ischemic tolerance. In vitro and in vivo assays indicated that the induced IRAK-M expression was localized in microglia. In addition, TLR4 specific inhibitor TAK-242 abolished the neuroprotective effects and the induction of IRAK-M offered by rHMGB1 preconditioning. Collectively, our study demonstrates that rHMGB1 preconditioning is neuroprotective during cerebral IRI, which is associated with activated TLR4/IRAK-M signaling in microglia. We found that high-mobility group box 1 (HMGB1) pretreatment conditioned the brain against subsequent ischemia-reperfusion injury. We propose the following mechanism for HMGB1 preconditioning-mediated ischemic tolerance: through toll-like receptor TLR4, HMGB1 preconditioning magnifies the up-regulation of interleukin-1R-associated kinase-M (IRAK-M) induced by ischemia-reperfusion in microglia, resulting in the decreased phosphorylation of IRAK-1. These findings are helpful in understanding the endogenous mechanisms that counteract ischemic insults.
- Published
- 2016
4. Heme activates TLR4-mediated inflammatory injury via MyD88/TRIF signaling pathway in intracerebral hemorrhage
- Author
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Lin Sen, Yin Qing, Zhong Qi, Lv Feng-Lin, Zhou Yu, Li Jing-Qi, Wang Jing-Zhou, Su Bing-yin, and Yang Qing-Wu
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Toll-like receptor 4 ,MyD88 ,TRIF ,Inflammation ,Intracerebral hemorrhage ,Heme ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background Inflammatory injury plays a critical role in intracerebral hemorrhage (ICH)-induced neurological deficits; however, the signaling pathways are not apparent by which the upstream cellular events trigger innate immune and inflammatory responses that contribute to neurological impairments. Toll-like receptor 4 (TLR4) plays a role in inflammatory damage caused by brain disorders. Methods In this study, we investigate the role of TLR4 signaling in ICH-induced inflammation. In the ICH model, a significant upregulation of TLR4 expression in reactive microglia has been demonstrated using real-time RT-PCR. Activation of microglia was detected by immunohistochemistry, cytokines were measured by ELISA, MyD88, TRIF and NF-κB were measured by Western blot and EMSA, animal behavior was evaluated by animal behavioristics. Results Compared to WT mice, TLR4−/− mice had restrained ICH-induced brain damage showing in reduced cerebral edema and lower neurological deficit scores. Quantification of cytokines including IL-6, TNF-α and IL-1β and assessment of macrophage infiltration in perihematoma tissues from TLR4−/−, MyD88−/− and TRIF−/− mice showed attenuated inflammatory damage after ICH. TLR4−/− mice also exhibited reduced MyD88 and TRIF expression which was accompanied by decreased NF-κB activity. This suggests that after ICH both MyD88 and TRIF pathways might be involved in TLR4-mediated inflammatory injury possibly via NF-κB activation. Exogenous hemin administration significantly increased TLR4 expression and microglial activation in cultures and also exacerbated brain injury in WT mice but not in TLR4−/− mice. Anti-TLR4 antibody administration suppressed hemin-induced microglial activation in cultures and in the mice model of ICH. Conclusions Our findings suggest that heme potentiates microglial activation via TLR4, in turn inducing NF-κB activation via the MyD88/TRIF signaling pathway, and ultimately increasing cytokine expression and inflammatory injury in ICH. Targeting TLR4 signaling may be a promising therapeutic strategy for ICH.
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- 2012
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5. Promoting blood circulation for removing blood stasis therapy for acute intracerebral hemorrhage: a systematic review and meta-analysis
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Hui-qin Li, Chen Wang, Jing-jing Wei, Yan Wang, Ji-ping Fan, Liang Song, Ji-huang Li, Wan Xia, Guo-Qing Zheng, Su-bing Yin, and Ai-ju Liu
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medicine.medical_specialty ,Review ,Blood stasis ,Hematoma ,Risk Factors ,Internal medicine ,Odds Ratio ,medicine ,Adjuvant therapy ,Humans ,Pharmacology (medical) ,Medicine, Chinese Traditional ,Adverse effect ,Cerebral Hemorrhage ,Pharmacology ,Intracerebral hemorrhage ,Chi-Square Distribution ,Evidence-Based Medicine ,Traditional medicine ,business.industry ,General Medicine ,Odds ratio ,medicine.disease ,Clinical trial ,Drug Combinations ,Treatment Outcome ,Cerebrovascular Circulation ,Meta-analysis ,business ,Drugs, Chinese Herbal - Abstract
To conduct a systematic review and meta-analysis to assess the current evidence available regarding the promoting blood circulation and removing blood stasis (PBCRBS) therapy for Chinese patients with acute intracerebral hemorrhage (ICH). Six databases were searched from their inception to November 2013. The studies assessed in ≥4 domains with 'yes' were selected for detailed assessment and meta-analysis. The herbal compositions for PBCRBS therapy for acute ICH patients were also assessed. From the 6 databases, 292 studies claimed randomized-controlled clinical trials (RCTs). Nine studies with 798 individuals were assessed in ≥4 domains with 'yes' by using the Cochrane RoB tool. Meta-analysis showed that PBCRBS monotherapy and adjuvant therapy for acute ICH could improve the neurological function deficit, reduce the volume of hematoma and perihematomal edema, and lower the mortality rate and dependency. Moreover, there were fewer adverse effects when compared with Western conventional medication controls. Xueshuantong Injection and Fufang Danshen Injection, Buyang Huanwu Decoction and Liangxue Tongyu formula, and three herbs (danshen root, sanqi and leech) were the most commonly used Chinese herbal patent injections, herbal prescriptions and single herbs, respectively. Despite the apparently positive findings, it is premature to conclude that there is sufficient efficacy and safety of PBCRBS for ICH because of the high clinical heterogeneity of the included studies and small number of trials in the meta-analysis. Further large sample-sizes and rigorously designed RCTs are needed.
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- 2015
6. Adenosine A2A Receptor Gene Knockout Prevents l-3,4-Dihydroxyphenylalanine-Induced Dyskinesia by Downregulation of Striatal GAD67 in 6-OHDA-Lesioned Parkinson’s Mice
- Author
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Su-bing Yin, Xia-wei Zheng, Xiao-guang Zhang, Wen-ting Yang, Shuang Chen, and Guo-Qing Zheng
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0301 basic medicine ,medicine.medical_specialty ,Parkinson's disease ,striatum ,Adenosine A2A receptor ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Dopamine ,Internal medicine ,medicine ,Gene knockout ,adenosine A2A receptor ,glutamic acid decarboxylase ,medicine.disease ,Dihydroxyphenylalanine ,Adenosine ,Abnormal involuntary movement ,dyskinesia ,030104 developmental biology ,Endocrinology ,chemistry ,Dyskinesia ,Neurology ,Parkinson’s disease ,Neurology (clinical) ,medicine.symptom ,030217 neurology & neurosurgery ,medicine.drug ,Neuroscience - Abstract
l-3,4-Dihydroxyphenylalanine (l-DOPA) remains the primary pharmacological agent for the symptomatic treatment of Parkinson’s disease (PD). However, the development of l-DOPA-induced dyskinesia (LID) limits the long-term use of l-DOPA for PD patients. Some data have reported that adenosine A2A receptor (A2AR) antagonists prevented LID in animal model of PD. However, the mechanism in which adenosine A2AR blockade alleviates the symptoms of LID has not been fully clarified. Here, we determined to knock out (KO) the gene of A2AR and explored the possible underlying mechanisms implicated in development of LID in a mouse model of PD. A2AR gene KO mice were unilaterally injected into the striatum with 6-hydroxydopamine (6-OHDA) in order to damage dopamine neurons on one side of the brain. 6-OHDA-lesioned mice were then injected once daily for 21 days with l-DOPA. Abnormal involuntary movements (AIMs) were evaluated on days 3, 8, 13, and 18 after l-DOPA administration, and real-time polymerase chain reaction and immunohistochemistry for glutamic acid decarboxylase (GAD) 65 and GAD67 were performed. We found that A2AR gene KO was effective in reducing AIM scores and accompanied with decrease of striatal GAD67, rather than GAD65. These results demonstrated that the possible mechanism involved in alleviation of AIM symptoms by A2AR gene KO might be through reducing the expression of striatal GAD67.
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- 2017
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7. Adenosine A
- Author
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Su-Bing, Yin, Xiao-Guang, Zhang, Shuang, Chen, Wen-Ting, Yang, Xia-Wei, Zheng, and Guo-Qing, Zheng
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dyskinesia ,adenosine A2A receptor ,striatum ,Parkinson’s disease ,glutamic acid decarboxylase ,Neuroscience ,Original Research - Abstract
l-3,4-Dihydroxyphenylalanine (l-DOPA) remains the primary pharmacological agent for the symptomatic treatment of Parkinson’s disease (PD). However, the development of l-DOPA-induced dyskinesia (LID) limits the long-term use of l-DOPA for PD patients. Some data have reported that adenosine A2A receptor (A2AR) antagonists prevented LID in animal model of PD. However, the mechanism in which adenosine A2AR blockade alleviates the symptoms of LID has not been fully clarified. Here, we determined to knock out (KO) the gene of A2AR and explored the possible underlying mechanisms implicated in development of LID in a mouse model of PD. A2AR gene KO mice were unilaterally injected into the striatum with 6-hydroxydopamine (6-OHDA) in order to damage dopamine neurons on one side of the brain. 6-OHDA-lesioned mice were then injected once daily for 21 days with l-DOPA. Abnormal involuntary movements (AIMs) were evaluated on days 3, 8, 13, and 18 after l-DOPA administration, and real-time polymerase chain reaction and immunohistochemistry for glutamic acid decarboxylase (GAD) 65 and GAD67 were performed. We found that A2AR gene KO was effective in reducing AIM scores and accompanied with decrease of striatal GAD67, rather than GAD65. These results demonstrated that the possible mechanism involved in alleviation of AIM symptoms by A2AR gene KO might be through reducing the expression of striatal GAD67.
- Published
- 2016
8. Differentiation of rat adipose‑derived mesenchymal stem cells into corneal‑like epithelial cells driven by PAX6
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Sun, Jing, primary, Liu, Wei‑Hua, additional, Deng, Feng‑Mei, additional, Luo, Yong‑Hui, additional, Wen, Ke, additional, Zhang, Hong, additional, Liu, Hai‑Rong, additional, Wu, Jiang, additional, Su, Bing‑Yin, additional, and Liu, Yi‑Lun, additional
- Published
- 2017
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9. ProBDNF inhibits collective migration and chemotaxis of rat Schwann cells
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Ding, You-Quan, primary, Li, Xuan-Yang, additional, Xia, Guan-Nan, additional, Ren, Hong-Yi, additional, Zhou, Xin-Fu, additional, Su, Bing-Yin, additional, and Qi, Jian-Guo, additional
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- 2016
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10. Differentiation of rat adipose-derived mesenchymal stem cells into corneal-like epithelial cells driven by PAX6.
- Author
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Sun, Jing, Liu, Wei-Hua, Deng, Feng-Mei, Luo, Yong-Hui, Wen, Ke, Zhang, Hong, Liu, Hai-Rong, Wu, Jiang, Su, Bing-Yin, and Liu, Yi-Lun
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GENETICS of eye diseases ,MESENCHYMAL stem cells ,CORNEAL transplantation ,VISUAL acuity ,LABORATORY rats ,ANTISENSE DNA - Abstract
Corneal integrity, transparency and vision acuity are maintained by corneal epithelial cells (CECs), which are continuously renewed by corneal limbal stem cells (LSCs). Deficiency of CECs and/or LSCs is associated with numerous ocular diseases. Paired box (PAX)6 is an eye development-associated transcription factor that is necessary for cell fate determination and differentiation of LSCs and CECs. In the present study, the PAX6 gene was introduced into adipose-derived rat mesenchymal stem cells (ADMSCs) to investigate whether PAX6-transfected cells were able to transdifferentiate into corneal-like epithelial cells and to further verify whether the cells were suitable as a cell source for corneal transplantation. The ADMSCs were isolated from the bilateral inguinal region of healthy Sprague Dawley rats. The characteristics of ADMSCs were identified using flow cytometric analysis. After subculture, ADMSCs underwent transfection with recombinant plasmid containing either PAX6-enhanced green fluorescent protein (EGFP) complementary (c)DNA or EGFP cDNA (blank plasmid group), followed by selection with G418 and determination of the transfection efficiency. Subsequently, the morphology of the ADMSCs and the expression profiles of corneal-specific markers CK3/12 and epithelial-specific adhesion protein were determined. E-cadherin was detected using immunofluorescence staining and western blot analysis at 21 days following transfection. An MTT cell proliferation and a colony formation assay were performed to assess the proliferative activity and clonogenicity of PAX6-transfected ADMSCs. Finally, the PAX6-expressing ADMSCs were transplanted onto the cornea of a rabbits with limbal stem cell deficiency (LSCD). At 21 days after transfection, the ADMSCs with PAX6 transfection exhibited a characteristic flagstone-like appearance with assembled corneal-like epithelial cells, and concomitant prominent expression of the corneal-specific markers cytokeratin 3/12 and E-cadherin. Furthermore, the proliferation and colony formation ability of PAX6-overexpressing ADMSCs was significantly retarded. The transplantation experiment indicated that PAX6-reprogramed ADMSCs attached to and replenished the damaged cornea via formation of stratified corneal epithelium. Taken together, these results suggested that conversion of ADMSCs into corneal-like epithelium may be driven by PAX6 transfection, which makes ADMSCs a promising cell candidate for the treatment of LSCD. [ABSTRACT FROM AUTHOR]
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- 2018
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11. Dihuang Yinzi, a Classical Chinese Herbal Prescription, for Amyotrophic Lateral Sclerosis
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Hui Qiu, Su-bing Yin, Jiang-Qiong Ke, Ji-huang Li, Chang-lin Qiu, and Guo-Qing Zheng
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Adult ,medicine.medical_specialty ,Pediatrics ,Electromyography ,Traditional Chinese medicine ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,Tongue ,law ,medicine ,Humans ,Clinical Case Report ,Survivors ,Medical prescription ,Amyotrophic lateral sclerosis ,medicine.diagnostic_test ,business.industry ,Amyotrophic Lateral Sclerosis ,General Medicine ,medicine.disease ,Surgery ,Riluzole ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Female ,Choking ,business ,030217 neurology & neurosurgery ,Research Article ,Drugs, Chinese Herbal ,Follow-Up Studies ,Phytotherapy ,medicine.drug - Abstract
Amyotrophic lateral sclerosis (ALS) is a devastating progressive neurodegenerative disease with no effective treatment and death within 2 to 5 years after symptom onset. Here, we reported a case of ALS patient using modified Dihuang Yinzi (DHYZ), a classical traditional Chinese medicine (TCM) prescription, who has survived 12 years with significant improvement in bulbar paralysis. A 41-year-old Chinese Han nationality woman was admitted to the hospital with complaints of weakened bilateral grip, slurred speech, stumbling, and muscle twitching for 3 years. The electromyography showed neurogenic injury in bilateral upper limbs and tongue. She was diagnosed with ALS according to the revised El escorial criteria. The patient was orally administrated with Riluzole 100 mg daily for 10 months and then stopped. Subsequently, she resorted to TCM. Based on the TCM theory, the patient was diagnosed with Yinfei syndrome because of kidney deficiency. DHYZ was chosen because it has the function of replenishing kidney essence to treat Yinfei syndrome. Up to now, she has been using modified DHYZ continuously for 12 years. The patient survived with ALS and did not require permanent continuous ventilator. In addition, the symptoms of choking on liquids are improved, and the utility of 30 mL water swallow test was improved with grade 2. The symptoms of muscle fibrillations of limbs are also reduced. However, muscle strength worsened slowly. The repeated electromyography showed motor conduction amplitude reducing gradually and velocity not changing more when compared with the initial electromyography. Our findings suggested that DHYZ can be potentially used in ALS patients because of its multi-targeted neuroprotection and general safety, although ALS does not have a cure. In addition, we identified the area that is worthy of further study and DHYZ as a promising candidate for further clinical application and ALS trials. Rigorous randomized controlled trials are needed in the future.
- Published
- 2016
12. Adenosine A2A Receptor Gene Knockout Prevents L-3,4-Dihydroxyphenylalanine-Induced Dyskinesia by Downregulation of Striatal GAD67 in 6-OHDA-Lesioned Parkinson's Mice.
- Author
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Su-bing Yin, Xiao-guang Zhang, Shuang Chen, Wen-ting Yang, Xia-wei Zheng, and Guo-qing Zheng
- Subjects
ADENOSINES ,GENE knockout ,DYSKINESIAS - Abstract
L-3,4-Dihydroxyphenylalanine (L-DOPA) remains the primary pharmacological agent for the symptomatic treatment of Parkinson's disease (PD). However, the development of L-DOPA-induced dyskinesia (LID) limits the long-term use of L-DOPA for PD patients. Some data have reported that adenosine A
2A receptor (A2A R) antagonists prevented LID in animal model of PD. However, the mechanism in which adenosine A2A R blockade alleviates the symptoms of LID has not been fully clarified. Here, we determined to knock out (KO) the gene of A2A R and explored the possible underlying mechanisms implicated in development of LID in a mouse model of PD. A2A R gene KO mice were unilaterally injected into the striatum with 6-hydroxydopamine (6-OHDA) in order to damage dopamine neurons on one side of the brain. 6-OHDA-lesioned mice were then injected once daily for 21 days with L-DOPA. Abnormal involuntary movements (AIMs) were evaluated on days 3, 8, 13, and 18 after L-DOPA administration, and real-time polymerase chain reaction and immunohistochemistry for glutamic acid decarboxylase (GAD) 65 and GAD67 were performed. We found that A2A R gene KO was effective in reducing AIM scores and accompanied with decrease of striatal GAD67, rather than GAD65. These results demonstrated that the possible mechanism involved in alleviation of AIM symptoms by A2A R gene KO might be through reducing the expression of striatal GAD67. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
13. The quality of reporting of randomized controlled trials of electroacupuncture for stroke.
- Author
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Jing-jing Wei, Wen-ting Yang, Su-bing Yin, Chen Wang, Yan Wang, and Guo-qing Zheng
- Subjects
PUBLISHING ,CONFIDENCE intervals ,ELECTROACUPUNCTURE ,INFORMATION storage & retrieval systems ,MEDICAL databases ,MEDICAL information storage & retrieval systems ,MEDLINE ,ONLINE information services ,PROBABILITY theory ,RESEARCH funding ,T-test (Statistics) ,SYSTEMATIC reviews ,RANDOMIZED controlled trials ,DATA analysis software ,STROKE rehabilitation ,DESCRIPTIVE statistics ,STANDARDS - Abstract
Background: Electroacupuncture (EA), as an extension technique of acupuncture based on traditional acupuncture combined with modern electrotherapy, is commonly used for stroke in clinical treatment and researches. However, there is still a lack of enough evidence to recommend the routine use of EA for stroke. This study is aimed at evaluating the quality of reporting of randomized controlled trials (RCTs) on EA for stroke. Methods: RCTs on EA for stroke were evaluated by using CONSORT guidelines and STRICTA guidelines. Microsoft Excel 2010 and the R software were used for descriptive statistics analyses. Results: Seventy studies involving 5468 stroke patients were identified. The CONSORT scores ranged from 16.2 to 67.6% and STRICTA scores from 29.4 to 82.4%. The central items in CONSORT as eligibility criterion, sample size calculation, primary outcome, method of randomization sequence generation, allocation concealment, implementation of randomization, description of blinding, and detailed statistical methods were reported in 100, 6, 68, 37, 14, 10, 16, and 97% of trials, respectively. The reporting of items in STRICTA as acupuncture rationale was 1a (91%), 1b (86%) and 1c 0%; needling details 2a (33%), 2b (97%), 2c (29%), 2d (64%), 2e (100%), 2f (55%) and 2 g (66%); treatment regimen 3a (69%) and 3b (100%); other components of treatment 4a (86%) and 4b (13%); practitioner background item 5 (16%); control intervention(s) 6a (93%) and 6b (10%). Conclusions: The quality of reporting of RCTs on EA for stroke was generally moderate. The reporting quality needs further improvement. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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14. Immunolocalization of estrogen receptor beta in the hypothalamic paraventricular nucleus of female mice during pregnancy, lactation and postnatal development
- Author
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Zhang, Ji-Qiang, primary, Su, Bing-yin, additional, and Cai, Wen-Qin, additional
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- 2004
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15. Distribution and differences of estrogen receptor beta immunoreactivity in the brain of adult male and female rats
- Author
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Zhang, Ji-Qiang, primary, Cai, Wen-Qin, additional, Zhou, De-Shan, additional, and Su, Bing-Yin, additional
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- 2002
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16. A developmental study of novH gene expression in human central nervous system
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Su, Bing-yin, primary, Cai, Ween-qin, additional, Zhang, Cheng-gang, additional, Su, Hui-ci, additional, and Perbal, Bernard, additional
- Published
- 1998
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17. The quality of reporting of randomized controlled trials of electroacupuncture for stroke
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Jing-jing Wei, Wen-ting Yang, Yan Wang, Guo-Qing Zheng, Chen Wang, and Su-bing Yin
- Subjects
medicine.medical_specialty ,Randomization ,Blinding ,Electroacupuncture ,medicine.medical_treatment ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,law ,Acupuncture ,Medicine ,Humans ,030212 general & internal medicine ,Stroke ,Randomized Controlled Trials as Topic ,Dry needling ,business.industry ,Methodology ,Consolidated Standards of Reporting Trials ,General Medicine ,medicine.disease ,Complementary and alternative medicine ,Physical therapy ,business ,030217 neurology & neurosurgery ,Research Article - Abstract
Background Electroacupuncture (EA), as an extension technique of acupuncture based on traditional acupuncture combined with modern electrotherapy, is commonly used for stroke in clinical treatment and researches. However, there is still a lack of enough evidence to recommend the routine use of EA for stroke. This study is aimed at evaluating the quality of reporting of randomized controlled trials (RCTs) on EA for stroke. Methods RCTs on EA for stroke were evaluated by using CONSORT guidelines and STRICTA guidelines. Microsoft Excel 2010 and the R software were used for descriptive statistics analyses. Results Seventy studies involving 5468 stroke patients were identified. The CONSORT scores ranged from 16.2 to 67.6% and STRICTA scores from 29.4 to 82.4%. The central items in CONSORT as eligibility criterion, sample size calculation, primary outcome, method of randomization sequence generation, allocation concealment, implementation of randomization, description of blinding, and detailed statistical methods were reported in 100, 6, 68, 37, 14, 10, 16, and 97% of trials, respectively. The reporting of items in STRICTA as acupuncture rationale was 1a (91%), 1b (86%) and 1c 0%; needling details 2a (33%), 2b (97%), 2c (29%), 2d (64%), 2e (100%), 2f (55%) and 2 g (66%); treatment regimen 3a (69%) and 3b (100%); other components of treatment 4a (86%) and 4b (13%); practitioner background item 5 (16%); control intervention(s) 6a (93%) and 6b (10%). Conclusions The quality of reporting of RCTs on EA for stroke was generally moderate. The reporting quality needs further improvement.
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18. [Effect of Human Adipose Mesenchymal Stem Cells on Phenotype Polarization of Mice Microglia via TLR3/TRIF Signal Pathway].
- Author
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Sun J, Liu YL, Li C, Ma Y, Yan WH, and Su BY
- Subjects
- Adipose Tissue cytology, Animals, Cells, Cultured, Humans, Lipopolysaccharides, Mice, Mice, Inbred C57BL, Phenotype, Adaptor Proteins, Vesicular Transport metabolism, Cell Polarity, Mesenchymal Stem Cells cytology, Microglia cytology, Signal Transduction, Toll-Like Receptor 3 metabolism
- Abstract
Objective: To explore the effect and mechanism of human adipose-derived mesenchymal stem cells (hADMSCs) on phenotypic polarization of microglia., Methods: BV-2 microglia of C57/BL6 mice were co-cultured with hADMSCs+lipopolysaccharide (LPS), or cultured with LPS alone. Cell morphology was observed under an inverted microscope. The effect of hADMSCs on microglial proliferation was evaluated by CCK-8 assay. The impact of hADMSCs on microglia M1/M2 phenotype markers were detected using quantitative real-time PCR (RT-qPCR). The affect of hADMSCs on the proteins expression levels of Toll-like receptor 4 (TLR4)-TIR domain containing adaptor protein inducing interferon β (TRIF) signaling pathway in BV-2 microglia was detected by using Western blot analysis., Results: As compared with the LPS treatment, hADMSCs treatment had no obvious effect on microglia morphology, whereas showed significant inhibition on microglial proliferation activity ( P <0.05). Simultaneously, hADMSCs treatment reduced expression of microglia M1 phenotype markers ( P <0.05), and increased microglia M1 phenotype markers in gene levels ( P <0.05). At the same time, protein expression levels of TRIF, TLR4, phosphorylated interferon regulatory factor 3 (P-IRF3) and interferon regulatory factor 3 (IRF3) in BV-2 microglia were decreased after hADMSCs treatment., Conclusion: hADMSCs can blockade the LPS-induced pro-inflammatory microglia M1 phenotype, whereas induces protective microglial M2 phenotype, which may be related to inhibition of the TLR4-TRIF signaling pathway., (Copyright© by Editorial Board of Journal of Sichuan University (Medical Science Edition).)
- Published
- 2019
19. Poly-lactic acid and agarose gelatin play an active role in the recovery of spinal cord injury.
- Author
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Deng QY, Li SR, Cai WQ, and Su BY
- Abstract
Objective To investigate the role of poly-lactic acid and agarose gelatin in promoting the functional recovery of the injured spinal cord. Methods Poly-lactic acid (PLA) or agarose was embedded in the space between two stumps of the hemisectioned spinal cord. Immunohistochemistry was used to show astroglia proliferation and the infiltration of RhoA-positive cells. Locomotor activity recovery was evaluated by testing the function of hindlimbs. Results Astroglias and RhoA labeled non-neuronal cells accumulated in the area adjacent to the implant, while the number of RhoA-positive cells was decreased dramatically in the absence of implant. Animals implanted with agarose gelatin recovered more quickly than those with PLA, concomitant with a higher survival rate of the neurons. Conclusion Both PLA and agarose gelatin benefited the recovery of spinal cord after injury by providing a scaffold for astroglia processes. Modulation of the rigidity, pore size and inner structure of PLA and agarose gelatin might make these biodegradable materials more effective in the regeneration of the central nervous system (CNS).
- Published
- 2006
20. Immunocytochemical localization of estrogen receptor beta in the rat brain.
- Author
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Zhang JQ, Cai WQ, Su BY, and Zhou de S
- Subjects
- Animals, Female, Male, Rats, Rats, Wistar, Sex Factors, Brain metabolism, Estrogen Receptor beta metabolism
- Abstract
The localization of the new estrogen receptor, ER-beta, in the rat brain was studied by immunocytochemical technique, and the results revealed that ER-beta immunoactive material was predominantly localized in the neuronal nucleus, but it was also detectable in the cytoplasm and neuronal processes. High levels of ER-beta immunopositive signals were detected in the cerebral cortex, vertical limb of the diagonal band, Purkinje cells, locus ceruleus, and motor trigeminal nucleus. Moderate levels were found in the medial septum, lateral amygdaloid nucleus, substantia nigra, and central gray. Weak signals were localized in some subregions of the hypothalamus and amygdaloid complex. Some differences of the expression of ER-beta immunoreactivity between male and female rats were also noticed. The above results provide the first detailed evidence that ER-beta protein is widely distributed in the rat brain, and ER-beta may be involved in some important brain function such as learning and memory.
- Published
- 2002
21. [Progress in the study of CCN family].
- Author
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Su BY and Cai WQ
- Subjects
- Animals, CCN Intercellular Signaling Proteins, Cattle, Cell Differentiation physiology, Connective Tissue Growth Factor, Cysteine-Rich Protein 61, Forecasting, Humans, Immediate-Early Proteins chemistry, Insulin-Like Growth Factor Binding Proteins, Intercellular Signaling Peptides and Proteins chemistry, Intracellular Signaling Peptides and Proteins, Mice, Neoplasm Proteins chemistry, Neoplasm Proteins physiology, Neovascularization, Physiologic physiology, Nephroblastoma Overexpressed Protein, Proto-Oncogene Proteins, Rats, Repressor Proteins chemistry, Repressor Proteins physiology, Swine, Wound Healing physiology, Immediate-Early Proteins physiology, Intercellular Signaling Peptides and Proteins physiology, Oncogene Proteins
- Abstract
The last 5-6 years have seen the emergence of a new gene family that currently comprises CTGF, Cyr61, nov, elm1, HICP and WISP-3. The translational products of most CCN family members are secreted proteins that contain 343 to 381 amino-acid residues to compose 4 distinct structural modules, each having 38 conserved cysteine residues. These proteins have a variety of properties to affect the cellular behaviors such as growth, differentiation, adhesion and locomotion. They may play important roles in pregnancy, development and differentiation, angiogenesis, wound repair, fibrotic disorders, inflammation and tumor genesis.
- Published
- 2002
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