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Cuproptosis is the latest novel form of cell death. However, the relationship between asthma and cuproptosis is not fully understood. In this study, we screened differentially expressed cuproptosis-related genes from the Gene Expression Omnibus (GEO) database and performed immune infiltration analysis. Subsequently, patients with asthma were typed and analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG). Weighted gene co-expression network analysis (WGCNA) was performed to calculate the module-trait correlations, and the hub genes of the intersection were taken to construct machine learning (XGB, SVM, RF, GLM). Finally, we used TGF-β to establish a BEAS-2B asthma model to observe the expression levels of hub genes. Six cuproptosis-related genes were obtained. Immune-infiltration analysis shows that cuproptosis-related genes are associated with a variety of biological functions. We classified asthma patients into two subtypes based on the expression of cuproptosis-related genes and found significant Gene Ontology (GO) and immune function differences between the different subtypes. WGCNA selected 2 significant modules associated with disease features and typing. Finally, we identified TRIM25, DYSF, NCF4, ABTB1, CXCR1 as asthma biomarkers by taking the intersection of the hub genes of the 2 modules and constructing a 5-genes signature, which nomograph, decision curve analysis (DCA) and calibration curves, receiver operating characteristic curve (ROC) showed high efficiency in diagnosing the probability of survival of asthma patients. Finally, in vitro experiments have shown that DYSF and CXCR1 expression is up expressed in asthma. Our study provides further directions for studying the molecular mechanism of asthma. [ABSTRACT FROM AUTHOR]

Polylactic acid (PLA) has become a popular polymer material due to its superior biocompatibility. At present, there are a few relevant research on heterogeneous bone powder. Besides, the poor dispersibility and adhesivity of inorganic particles in the organic phase remains a problem. In this study, the pork bone powders were modified with N‐butanol to improve its dispersibility and compatibility in the PLA matrix. In addition, polybutylene succinate‐co‐terephthalates (PBSA) was applied as a flexibilizer to further reinforce the mechanical properties of materials. The composite filaments with a diameter of 1.75 ± 0.05 mm containing 10 wt% of modified bone powder, 10 wt% PBSA and 80 wt% PLA were prepared by a melt blending method. The obtained results showed that modified particles were uniformly dispersed within the PLA matrix and improved the mechanical properties of the composite filaments with a tensile strength of 48.5 ± 0.2 MPa and a bending strength of 79.1 ± 0.1 MPa and a notch impact strength of 15.8 ± 0.3 kJ/m2. And the prepared composite materials contained low cytotoxicity, high biocompatibility and printability, which verified the feasibility of it in 3D printing personalized bone repair applications. This provides a theoretical basis for further research on the effect of bone repair in vivo. Therefore, the composite material will have potential applications such as making customized bones and bone scaffolds by three dimensional printing technology. [ABSTRACT FROM AUTHOR]