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To study the prevalence and isoforms of the pathogenicity island ETT2 among pathogenic Escherichia coli, as well as to determine the relationship between the ETT2 locus and other virulence factors, PCR amplifications target to the 35 ETT2-associated genes were established and used to investigate the presence of the ETT2 locus in 168 E. coli isolates from weaned piglets with edema and/or diarrhea or dairy cows with mastitis. The results showed that the ETT2 locus could be identified in the pathogenic E. coli isolates from colibacillosis in pigs and in the ones from mastitis in cows, but the presence of ETT2 among the isolates of porcine origin were significantly higher (85.87%) than that (47.37%) of bovine origin. Furthermore, 11 ETT2 isoforms were found in this research, including an intact form and 10 deletion types. The intact ETT2 was the prevalent form among the pathogenic E. coli isolates of porcine origin, and highly associated with the presence of shigatoxin type 2e (Stx2e), while the great majority isolates of bovine origin just carried various deletion types, and no distinct association with other virulence factors, e.g., the presence/absence of LT1, ST2, Cnf2, Tra, HPI, Hly, and F17a fimbriae. [ABSTRACT FROM AUTHOR]

Multi-wavelength distributed feedback laser array (DFB-MLA) based tunable semiconductor lasers have been widely used owing to the high mode stability and simple wavelength tuning scheme. In this paper, we proposed and experimentally demonstrated a matrix-grating tunable DFB laser array. And the reconstruction-equivalent-chirp (REC) technique is utilized to simplify grating fabrication and realize precise control of the grating phase. Traditionally, the laser is tuned by thermal-electric cooler (TEC), with large power consumption, slow wavelength tuning capability, and added device. To improve the wavelength tuning speed of laser, we varied laser’s injection current instead of adjusting TEC’s temperature. In addition, we analyzed the thermal influence of submount on the laser and then chose the SiO2 as the submount material for its low thermal conductivity and poor heat dissipation, resulting in wide wavelength tuning range via injection current. The DFB-MLA chip achieves a better tunable effect as the lasers can be tuned at 3.5–3.7 nm per channel from 50 to 120 mA with stable output power of over 3 mw as contrasted with the laser on the AlN submount being tuned at 1.4–1.5 nm per channel. And the chip has a side-mode suppressing ratio (SMSR) of over 35 dB and strong stability of wavelength in the repeated experiments. The proposed method may give a new way to realize the wide tuning range tunable DFB laser with a simple wavelength tuning mechanism in the future, especially for the applications of uncooled low-cost tunable lasers. [ABSTRACT FROM AUTHOR]