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Marine sponges are important sources of chemical variety and repository of biodiversity. In this study, the microbial communities found in the Spongia officinalis that was taken from the Kanyakumari coast in India were explored. We identified, characterised, and evaluated the bioactive potential of the sponge-associated bacteria. A total of 12 bacterial isolates were obtained, primarily consisting of gram-positive rods (7 isolates) and some gram-negative rods (2 isolates), and cocci (1 isolate). Among these KKS6 showed tremendous radical scavenging activity (85.16 ±1%) with a minimum inhibitory concentration as 167.26±0.1 µg/mL at the highest concentration when compared to other extracts. With an IC50 value of 55.32 g/mL, this isolate also displayed impressive anticancer activity against HeLa cells. The screened isolate was identified as Pseudomonas fluorescens strain using 16S rRNA sequencing. This discovery emphasises the importance of bacteria associated with Spongia officinalis as a source of bioactive compounds with medicinal potential. This study highlights the novel findings of diverse microbial communities found in Spongia officinalis and their potential for use in biotechnology and medication development. Pseudomonas fluorescens was found to be a prolific generator of bioactive byproducts, including strong antioxidants and anticancer agents, which could be a potent drug molecule in future anticancer research. [ABSTRACT FROM AUTHOR]