Search

NiCo2O4 (NCO) films grown on MgAl2O4 (001) substrates have been studied using magnetometry and x-ray magnetic circular dichroism based on x-ray absorption spectroscopy and spin-polarized inverse photoemission spectroscopy with various thicknesses down to 1.6 nm. The magnetic behavior can be understood in terms of a layer of optimal NCO and an interfacial layer (1.2 ± 0.1 nm), with a small canting of magnetization at the surface. The thickness dependence of the optimal layer can be described by the finite-scaling theory with a critical exponent consistent with the high perpendicular magnetic anisotropy. The interfacial layer couples antiferromagnetically to the optimal layer, generating exchange-spring styled magnetic hysteresis in the thinnest films. The non-optimal and measurement-speed-dependent magnetic properties of the interfacial layer suggest substantial interfacial diffusion. [ABSTRACT FROM AUTHOR]

Charcoal rot caused by the fungal pathogen Macrophomina phaseolina is an important disease of soybean and the use of resistant cultivars is recommended to manage the disease. Since symptoms, including leaf wilt, typically occur as soybeans reach maturity, screening varieties for tolerance to charcoal rot can be time-consuming, requiring nearly an entire growing season. In this study, soybean seedlings (V1) were exposed to a culture filtrate of M. phaseolina containing toxin(s) produced by the fungal pathogen. The effect on the seedlings was measured with hyperspectral spectroscopy on leaves. Two spectrometers integrated with a fiber optic light source and a 6.35 mm diameter probe yielding 480–850 nm and 900–2400 nm ranges after preprocessing were used. The spectra of the untreated plants measured at 0 h, 4 h, 8 h, 12 h, and 24 h post-exposure to the fungal extract were nearly indistinguishable. In contrast, the toxin-treated plants had significantly different spectra from the untreated plants at each of the 4 h, 8 h, 12 h, and 24 h measurements. Reflectance increased in the NIR (900–2400 nm) region with extended exposure to the fungal extract. This change was most noticeable in the 1450 nm and 1940 nm wavebands. Across the spectra, the 24 h reflectance was significantly higher than that of 12 h, which was significantly higher than those of 8 h, 4 h, and 0 h. Jeffries-Matusita (JM) distance, quantifying class separability, was used as a feature selection method and the 24 h measurement had the highest JM distance values, which indicated good separability. Based on JM Distance the most sensitive wavebands were in the regions of 1370–2400 nm. A ratio of the reflectance at 0 h to reflectance at the other times for each of the wavebands was calculated. The ratio curves had two noticeable troughs centered on 1450 nm and 1940 nm, with respective ratios of 0.47 and 0.32 for the 24 h measurement. The 1940 nm ratio at 24 h was proposed as a relative measure of charcoal rot susceptibility of soybean varieties. A ratio of 1.0 indicated no susceptibility with lower ratios indicating greater susceptibility to charcoal rot toxin(s). This study has implications in terms of developing tools to screen for soybean varieties tolerant to charcoal rot and potentially for other biotic or abiotic factors that induce foliar wilting. [ABSTRACT FROM AUTHOR]

Fusarium oxysporum,F. graminearum,F. acuminatum,F. equiseti,F. proliferatum,F. solani , and Rhizoctonia solani are soil-borne fungal pathogens that cause substantial yield loss in a widespread list of crops worldwide. The objective of this study was to develop a panel of TaqMan assays for the detection and quantification of these six widespread soil-borne fungal species using real-time polymerase chain reaction (qPCR). The primers and probes were designed based on the intergenic spacer ribosomal RNA and translation elongation factor 1-alpha gene (tef1). These assays, although not multiplexed, can be performed simultaneously as they have similar reaction conditions, allowing more efficiency when targeting multiple pathogens in a sample. The assays presented high efficiency (94.3%–108.9%) and sensitivity, with a limit of detection of 0.05 picograms (50 femtograms) of target DNA. Results from an assay targeting 19 non-target and closely related species confirmed the specificity of the developed assays. The assays were also evaluated to detect the target species in different matrices, such as soil and plant material. This panel of qPCR assays is an additional tool that can be used by plant pathologists, microbiologists, plant breeders, diagnostic clinics, and other researchers interested in these fungal species. [ABSTRACT FROM AUTHOR]