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1. High throughput quantification of capsular polysaccharides for multivalent vaccines using precipitation with a cationic surfactant.

Author

Noyes, Aaron, Boesch, Austin, Godavarti, Ranga, Titchener-Hooker, Nigel, Coffman, Jonathan, and Mukhopadhyay, Tarit

Subjects
*POLYSACCHARIDES, *CATIONIC surfactants, *VACCINE manufacturing, *ELECTROSTATIC precipitation, *TURBIDITY, *TITERS, *STANDARD deviations
Abstract

Highlights: [•] We describe titer assay for diverse set of anionic capsular polysaccharides. [•] Mechanism is electrostatic precipitation wherein turbidity is proportional to titer. [•] Characterized by two-log dynamic range and mean relative standard deviation <10%. [•] 96 samples can be measured in <30min with routine laboratory equipment. [•] Compatible with common buffers from pH 3.0 to 8.8 and with ≤400mM NaCl. [Copyright &y& Elsevier]

Published
2013
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