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20 results on '"Sugawara, Tae"'

3. Shape of the first mitotic spindles impacts nucleation in human embryos

Author

Ono, Yuki, primary, Shirasawa, Hiromitsu, additional, Kazumasa, Kazumasa, additional, Goto, Mayumi, additional, Ono, Takahiro, additional, Sakaguchi, Taichi, additional, Okabe, Motonari, additional, Hirakawa, Takeo, additional, Iwasawa, Takuya, additional, Fujishima, Akiko, additional, Onodera, Yohei, additional, Sugawara, Tae, additional, Makino, Kenichi, additional, Miura, Hiroshi, additional, Fukunaga, Noritaka, additional, Asada, Yoshimasa, additional, Kumazawa, Yukiyo, additional, and Terada, Yukihiro, additional

Published
2024
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11. Isolated loss of PMS2 immunohistochemical expression is frequently caused by heterogeneous MLH1 promoter hypermethylation in Lynch syndrome screening for endometrial cancer patients

Author

Kato, Aya, Sato, Naoki, Sugawara, Tae, Takahashi, Kazue, Kito, Masahiko, Makino, Kenichi, Sato, Toshiharu, Shimizu, Dai, Shirasawa, Hiromitu, Miura, Hiroshi, Sato, Wataru, Kumazawa, Yukiyo, Sato, Akira, Kumagai, Jin, Terada, Yukihiro, Kato, Aya, Sato, Naoki, Sugawara, Tae, Takahashi, Kazue, Kito, Masahiko, Makino, Kenichi, Sato, Toshiharu, Shimizu, Dai, Shirasawa, Hiromitu, Miura, Hiroshi, Sato, Wataru, Kumazawa, Yukiyo, Sato, Akira, Kumagai, Jin, and Terada, Yukihiro

Abstract

Lynch syndrome (LS) is an autosomal dominant inherited disorder mainly caused by a germline mutation in the DNA mismatch repair (MMR) genes (MLH1, MSH2, MSH6, PMS2) and is associated with increased risk of various cancers, particularly colorectal cancer and endometrial cancer (EC). Women with LS account for 2–6% of EC patients; it is clinically important to identify LS in such individuals for predicting and/or preventing additional LS-associated cancers. PMS2 germline mutation (PMS2-LS) is the rarest contribution to LS etiology among the four LS- associated MMR germline mutations, and its detection is complicated. Therefore, prudent screening for PMS2-LS is important as it leads to an efficient LS-identification strategy. Immunohistochemistry (IHC) is recommended as a screening method for LS in EC. Isolated loss of PMS2 expression (IL- PMS2) is caused not only by PMS2-LS but also by MLH1 germline mutation or MLH1 promoter hypermethylation (MLH-PHM). This study aimed to determine the association between MLH1-PHM and IL-PMS2 to avoid inappropriate genetic analysis. We performed MLH1 methylation analysis and MLH1/PMS2 germline mutation testing for the IL-PMS2 cases. By performing MMR-IHC for 360 unselected ECs, we selected eight (2.2%) cases as IL-PMS2. Heterogeneous MLH1 staining and MLH1-PHM were detected in 4/8 (50%) IL-PMS2 tumors. Out of five IL-PMS2 patients who underwent genetic analysis, one had PMS2 germline mutation with normal MLH1 expression (without MLH1-PHM) and no MLH1 germline mutation was detected. We suggest that MLH1 promoter methylation analysis for IL-PMS2 EC should be performed to exclude sporadic cases prior to further PMS2 genetic testing.

Published
2017

13. Isolated Loss of PMS2 Immunohistochemical Expression is Frequently Caused by Heterogenous MLH1 Promoter Hypermethylation in Lynch Syndrome Screening for Endometrial Cancer Patients

Author

Kato, Aya, primary, Sato, Naoki, additional, Sugawara, Tae, additional, Takahashi, Kazue, additional, Kito, Masahiko, additional, Makino, Kenichi, additional, Sato, Toshiharu, additional, Shimizu, Dai, additional, Shirasawa, Hiromistu, additional, Miura, Hiroshi, additional, Sato, Wataru, additional, Kumazawa, Yukiyo, additional, Sato, Akira, additional, Kumagai, Jin, additional, and Terada, Yukihiro, additional

Published
2016
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15. CRP 1846C>T Genetic Polymorphism Is Associated with Lymph Node Metastasis and/or Severe Lymphatic Invasion in Endometrial Cancer

Author

Kito, Masahiko, primary, Motoyama, Satoru, additional, Fujita, Kazuma, additional, Miura, Masatomo, additional, Nanjo, Hiroshi, additional, Sato, Naoki, additional, Shimizu, Dai, additional, Sato, Toshiharu, additional, Makino, Kenichi, additional, Sugawara, Tae, additional, Kato, Aya, additional, Tamura, Daisuke, additional, Takahashi, Kazue, additional, Kumazawa, Yukiyo, additional, Sato, Wataru, additional, Miura, Hiroshi, additional, Shirasawa, Hiromitsu, additional, Sato, Akira, additional, Kumagai, Jin, additional, and Terada, Yukihiro, additional

Published
2015
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18. Isolated Loss of PMS2 Immunohistochemical Expression is Frequently Caused by Heterogenous MLH1Promoter Hypermethylation in Lynch Syndrome Screening for Endometrial Cancer Patients

Author

Kato, Aya, Sato, Naoki, Sugawara, Tae, Takahashi, Kazue, Kito, Masahiko, Makino, Kenichi, Sato, Toshiharu, Shimizu, Dai, Shirasawa, Hiromistu, Miura, Hiroshi, Sato, Wataru, Kumazawa, Yukiyo, Sato, Akira, Kumagai, Jin, and Terada, Yukihiro

Abstract

Lynch syndrome (LS) is an autosomal-dominant inherited disorder mainly caused by a germline mutation in the DNA mismatch repair (MMR) genes (MLH1, MSH2, MSH6, and PMS2) and is associated with increased risk for various cancers, particularly colorectal cancer and endometrial cancer (EC). Women with LS account for 2% to 6% of EC patients; it is clinically important to identify LS in such individuals for predicting and/or preventing additional LS-associated cancers. PMS2germline mutation (PMS2-LS) is the rarest contribution to LS etiology among the 4 LS-associated MMR germline mutations, and its detection is complicated. Therefore, prudent screening for PMS2-LS is important as it leads to an efficient LS identification strategy. Immunohistochemistry is recommended as a screening method for LS in EC. Isolated loss of PMS2 (IL-PMS2) expression is caused not only by PMS2-LS but also by MLH1germline mutation or MLH1promoter hypermethylation (MLH-PHM). This study aimed to determine the association between MLH1-PHM and IL-PMS2 to avoid inappropriate genetic analysis. We performed MLH1methylation analysis and MLH1/PMS2germline mutation testing on the IL-PMS2 cases. By performing MMR-immunohistochemistry on 360 unselected ECs, we could select 8 (2.2%) cases as IL-PMS2. Heterogenous MLH1 staining and MLH1-PHM were detected in 4 of 8 (50%) IL-PMS2 tumors. Of the 5 IL-PMS2 patients who underwent genetic analysis, 1 had PMS2germline mutation with normal MLH1 expression (without MLH1-PHM), and no MLH1germline mutation was detected. We suggest that MLH1promoter methylation analysis for IL-PMS2 EC should be performed to exclude sporadic cases before further PMS2genetic testing.

Published
2016
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20. Repair of Uterine Rupture during Second Trimester Leading to Successful Pregnancy Outcome: Case Study and Literature's Review.

Author

Sugawara T, Ogawa M, and Tanaka T

Abstract

It was thought to be impossible to prolong the pregnancy in a case of uterine rupture in the second trimester. We encountered a case of rupture of the pregnant uterus in early mid-trimester, which we repaired with suture and overlapping of collagen fleece coated with fibrin glue, resulting in pregnancy prolongation until the 34th week. Our case and five previously reported cases were reviewed. Pregnant uterine rupture in mid-trimester could be repaired with suture and overlapping of collagen fleece in the absence of placenta percreta.

Published
2014
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