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A total of 99 dual soundings with Meisei iMS-100 radiosonde and Vaisala RS92 radiosondes were carried out at the Aerological Observatory of the Japan Meteorological Agency, known as Tateno (36.06∘ N, 140.13∘ E, 25.2 m; the World Meteorological Organization, WMO, station number 47646), from September 2017 to January 2020. Global Climate Observing System (GCOS) Reference Upper-Air Network (GRUAN) data products (GDPs) from both sets of radiosonde data for 59 flights were subsequently created using a documented processing programme along with the provision of optimal estimates for measurement uncertainty. Differences in radiosonde performance were then quantified using these GDPs. For daytime observations, the iMS-100 temperature is around 0.5 K cooler than RS92-GDP in the stratosphere, with significant differences in the upper troposphere and lower stratosphere in consideration of combined uncertainties. For nighttime observations, the difference is around −0.1 K, and data are mostly in agreement. For relative humidity (RH), iMS-100 is around 1 % RH–2 % RH higher in the troposphere and 1 % RH smaller in the stratosphere than RS92, but both GDPs are in agreement for most of the profile. The mean pressure difference is ≤0.1 hPa, the wind speed difference is from −0.04 to +0.14 m s−1, the wind direction difference is ≤6.4∘, and the root mean square vector difference (RMSVD) for wind is ≤1.04 m s−1.

A total of 87 dual flights of Meisei RS-11G radiosondes and Vaisala RS92-SGP radiosondes were carried out at the Aerological Observatory of the Japan Meteorological Agency (36.06∘ N, 140.13∘ E, 25.2 m) from April 2015 to June 2017. Global Climate Observing System (GCOS) Reference Upper-Air Network (GRUAN) data products from both sets of radiosonde data for 52 flights were subsequently created using a documented processing program along with the provision of optimal estimates for measurement uncertainty. Differences in the performance of the radiosondes were then quantified using the GRUAN data products. The temperature measurements of RS-11G were, on average, 0.4 K lower than those of RS92-SGP in the stratosphere for daytime observations. The relative humidity measurements of RS-11G were, on average, 2 % RH (relative humidity) lower than those of RS92-SGP under 90 % RH–100 % RH conditions, while RS-11G gave on average 5 % RH higher values than RS92-SGP under ≤50 % RH conditions. The results from a dual flight of RS-11G and a cryogenic frost point hygrometer (CFH) also showed that RS-11G gave 1 % RH–10 % RH higher values than the CFH in the troposphere. Differences between the RS-11G and RS92-SGP temperature and relative humidity measurements, based on combined uncertainties, were also investigated to clarify major influences behind the differences. It was found that temperature differences in the stratosphere during daytime observation were within the range of uncertainty (k=2), and that sensor orientation is the major source of uncertainty in the RS92-SGP temperature measurement, while sensor albedo is the major source of uncertainty for RS-11G. The relative humidity difference in the troposphere was larger than the uncertainty (k=2) after the radiosondes had passed through the cloud layer, and the temperature–humidity dependence correction was the major source of uncertainty in RS-11G relative humidity measurement. Uncertainties for all soundings were also statistically investigated. Most nighttime temperature measurements for pressures of >10 hPa were in agreement, while relative humidity in the middle troposphere exhibited significant differences. Around half of all daytime temperature measurements at pressures of ≤150 hPa and relative humidity measurements around the 500 hPa level were not in agreement.

A meteorological balloon-borne cloud sensor called the cloud particle sensor (CPS) has been developed. The CPS is equipped with a diode laser at ∼ 790 nm and two photodetectors, with a polarization plate in front of one of the detectors, to count the number of particles per second and to obtain the cloud-phase information (i.e. liquid, ice, or mixed). The lower detection limit for particle size was evaluated in laboratory experiments as ∼ 2 µm diameter for water droplets. For the current model the output voltage often saturates for water droplets with diameter equal to or greater than ∼ 80 µm. The upper limit of the directly measured particle number concentration is ∼ 2 cm−3 (2 × 103 L−1), which is determined by the volume of the detection area of the instrument. In a cloud layer with a number concentration higher than this value, particle signal overlap and multiple scattering of light occur within the detection area, resulting in a counting loss, though a partial correction may be possible using the particle signal width data. The CPS is currently interfaced with either a Meisei RS-06G radiosonde or a Meisei RS-11G radiosonde that measures vertical profiles of temperature, relative humidity, height, pressure, and horizontal winds. Twenty-five test flights have been made between 2012 and 2015 at midlatitude and tropical sites. In this paper, results from four flights are discussed in detail. A simultaneous flight of two CPSs with different instrumental configurations confirmed the robustness of the technique. At a midlatitude site, a profile containing, from low to high altitude, water clouds, mixed-phase clouds, and ice clouds was successfully obtained. In the tropics, vertically thick cloud layers in the middle to upper troposphere and vertically thin cirrus layers in the upper troposphere were successfully detected in two separate flights. The data quality is much better at night, dusk, and dawn than during the daytime because strong sunlight affects the measurements of scattered light.

Abstract Patients with acute coronary syndrome with diabetes mellitus (DM) exhibit an impaired platelet inhibitory response to clopidogrel which is only partially understood. DM was induced by the administration of streptozotocin (STZ) to 9-week-old mice. The antithrombotic effects of clopidogrel (10 mg/kg/d, orally × 5 days) were determined using a FeCl3-induced thrombosis model employing wild-type (WT), apolipoprotein E (apoE)-deficient, and diabetic apoE-deficient mice at 21 weeks. Antiplatelet effects were determined using flow cytometry. The antithrombotic effects of clopidogrel were similar in WT and apoE-deficient mice but were attenuated in diabetic apoE-deficient mice with the percent inhibition of thrombus area (µm2) by clopidogrel being 85.5% (WT mice), 75.0% (apoE-deficient mice), and 1.9% (diabetic apoE-deficient mice). The time to first occlusion and lumen stenosis also reflected a significant loss of the antithrombotic effects of clopidogrel in diabetic apoE-deficient mice. Ex vivo platelet activation, which was assessed using ADP-induced expression of activated glycoprotein IIb/IIIa, was completely inhibited by clopidogrel in these three groups of mice. In contrast, the effect of clopidogrel on the ex vivo expression of platelet P-selectin induced by protease-activated receptor 4–activating peptide was diminished in diabetic apoE-deficient mice compared with that in WT and apoE-deficient mice. These data suggest that diabetic apoE-deficient mice may serve as a useful model to better understand the impaired responses to clopidogrel in patients with DM, which may partially reflect a reduction of the effect of clopidogrel on thrombin-induced platelet activation.

BackgroundThe efficacy of P2Y12 inhibition for the prevention of cardiovascular events in patients with peripheral arterial disease (PAD) has been established. However, the therapeutic effects on ischemic limb complications are less clear. Accordingly, we aimed to develop a novel murine model of thrombotic hindlimb ischemia to reflect that found in patients with PAD exhibiting ischemic limb symptoms. We further investigated the effects of P2Y12 deficiency and P2Y12 inhibition by prasugrel in this model. Methods and ResultsThrombus formation induced by application of ferric chloride to the femoral artery resulted in a significant reduction in blood flow in the injured limb. In gait analysis using the CatWalk system, moderate difficulties in grounding and weight bearing of the ischemic limb, including reduction of maximum contact area and stance phase duration and increasing in swing phase duration in the ischemic limb, were observed in this model. Blood flow reduction and gait abnormalities gradually recovered over 21 days to levels present before arterial injury. Compared to wild‐type (WT) mice, significant increases in blood flow and improvement in gait were observed in P2Y12‐deficient mice. In addition, daily oral administration of prasugrel (3 mg/kg per day) to WT mice resulted in significant inhibition of blood flow reduction and gait abnormalities to levels found in P2Y12 deficient mice. ConclusionsAcute femoral artery thrombosis resulted in hindlimb ischemia and moderate gait abnormalities in mice. In addition, the present study suggests a possible role of P2Y12 in the complications with thrombotic limb ischemia.

Platelets play pivotal roles in both hemostasis and thrombosis. Although models of intravital platelet imaging are available for thrombosis studies in mice, few are available for rat studies. The present effort aimed to generate fluorescent platelets in rats and assess their dynamics in a rat model of arterial injury. We generated CD41-ZsGreen1 transgenic rats, in which green fluorescence protein ZsGreen1 was expressed specifically in megakaryocytes and thus platelets. The transgenic rats exhibited normal hematological and biochemical values with the exception of body weight and erythroid parameters, which were slightly lower than those of wild-type rats. Platelet aggregation, induced by 20 μM ADP and 10 μg/ml collagen, and blood clotting times were not significantly different between transgenic and wild-type rats. Saphenous arteries of transgenic rats were injured with 10% FeCl3, and the formation of fluorescent thrombi was evaluated using confocal microscopy. FeCl3 caused time-dependent increases in the mean fluorescence intensity of injured arteries of vehicle-treated rats. Prasugrel (3 mg/kg, p.o.), administered 2 h before FeCl3, significantly inhibited fluorescence compared with vehicle-treated rats (4.5 ± 0.4 vs. 14.9 ± 2.4 arbitrary fluorescence units at 30 min, respectively, n = 8, P = 0.0037). These data indicate that CD41-ZsGreen1 transgenic rats represent a useful model for intravital imaging of platelet-mediated thrombus formation and the evaluation of antithrombotic agents.

A new particle imaging radiosonde "Rainscope" has been developed, and for the first time, particle fall velocity measurement functionality was added to a balloon-borne device. Rainscope can capture a clear still image of precipitation particles in a cloud when they interrupt an infrared beam, using a CMOS camera equipped with an electronic shutter. It can also record the time when a particle passes the upper and lower built-in infrared sensors, enabling measurement of the velocity of falling precipitation particles. For ground testing in rain and snow, a ground-based Rainscope showed raindrop/snowflake size-fall velocity distributions similar to those obtained in previous studies. In a comparison with a Parsivel2 disdrometer in rain, the Rainscope results were in good agreement with the distributions obtained by an adjacent Parsivel2. In a test flight of Rainscope into a stratiform cloud, raindrops, mostly melted particles, snowflakes in the process of melting, graupel, and snowflakes were observed. It was observed that the fall velocity varied depending on the type of solid precipitation particles. [ABSTRACT FROM AUTHOR]