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5 results on '"Sumbatyan, Nataliya V."'

1. An easy tool to monitor the elemental steps of in vitro translation via gel electrophoresis of fluorescently labeled small peptides

Author

Marina, Valeriya I., primary, Bidzhieva, Medina, additional, Tereshchenkov, Andrey G., additional, Orekhov, Dmitry, additional, Sagitova, Vladislava E., additional, Sumbatyan, Nataliya V., additional, Tashlitsky, Vadim N., additional, Ferberg, Artem S., additional, Maviza, Tinashe P., additional, Kasatsky, Pavel, additional, Tolicheva, Olga, additional, Paleskava, Alena, additional, Polshakov, Vladimir I., additional, Osterman, Ilya A., additional, Dontsova, Olga A., additional, Konevega, Andrey L., additional, and Sergiev, Petr V., additional

Published
2023
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2. An easy tool to monitor the elemental steps of in vitro translation via gel electrophoresis of fluorescently labeled small peptides

Author

Marina, Valeriya I., Bidzhieva, Medina, Tereshchenkov, Andrey G., Orekhov, Dmitry, Sagitova, Vladislava E., Sumbatyan, Nataliya V., Tashlitsky, Vadim N., Ferberg, Artem S., Maviza, Tinashe P., Kasatsky, Pavel, Tolicheva, Olga, Paleskava, Alena, Polshakov, Vladimir I., Osterman, Ilya A., Dontsova, Olga A., Konevega, Andrey L., and Sergiev, Petr V.

Abstract

Several methods are available to visualize and assess the kinetics and efficiency of elemental steps of protein biosynthesis. However, each of these methods has its own limitations. Here, we present a novel, simple and convenient tool for monitoring stepwise in vitro translation initiated by BODIPY-Met-tRNA. Synthesis and release of very short, 1–7 amino acids, BODIPY-labeled peptides, can be monitored using urea–polyacrylamide gel electrophoresis. Very short BODIPY-labeled oligopeptides might be resolved this way, in contrast to widely used Tris-tricine gel electrophoresis, which is suitable to separate peptides larger than 1 kDa. The method described in this manuscript allows one to monitor the steps of translation initiation, peptide transfer, translocation, and termination as well as their inhibition at an unprecedented single amino acid resolution.

Published
2024
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4. Penetrating cation/fatty acid anion pair as a mitochondria-targeted protonophore

Author

Severin, Fedor F., primary, Severina, Inna I., additional, Antonenko, Yury N., additional, Rokitskaya, Tatiana I., additional, Cherepanov, Dmitry A., additional, Mokhova, Elena N., additional, Vyssokikh, Mikhail Yu., additional, Pustovidko, Antonina V., additional, Markova, Olga V., additional, Yaguzhinsky, Lev S., additional, Korshunova, Galina A., additional, Sumbatyan, Nataliya V., additional, Skulachev, Maxim V., additional, and Skulachev, Vladimir P., additional

Published
2009
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