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11 results on '"Sun, Chaoqin"'

5. In vitro Antifungal Activity of a Novel Antimicrobial Peptide AMP-17 Against Planktonic Cells and Biofilms of Cryptococcus neoformans

Author

Yang,Longbing, Tian,Zhuqing, Zhou,Luoxiong, Zhu,Lijuan, Sun,Chaoqin, Huang,Mingjiao, Peng,Jian, Guo,Guo, Yang,Longbing, Tian,Zhuqing, Zhou,Luoxiong, Zhu,Lijuan, Sun,Chaoqin, Huang,Mingjiao, Peng,Jian, and Guo,Guo

Abstract

Longbing Yang,1 Zhuqing Tian,1 Luoxiong Zhou,1,2 Lijuan Zhu,1 Chaoqin Sun,1 Mingjiao Huang,1 Jian Peng,1,3 Guo Guo1,3,4 1School of Basic Medical Sciences, The Key and Characteristic Laboratory of Modern Pathogen Biology, Guizhou Medical University, Guiyang, 550025, People’s Republic of China; 2School of Public Health, Guizhou Medical University, Guiyang, 550025, People’s Republic of China; 3Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, 550025, People’s Republic of China; 4Translational Medicine Research Center, Guizhou Medical University, Guiyang, 550025, People’s Republic of ChinaCorrespondence: Guo Guo, Building Wuben, School of Basic Medical Sciences, Guizhou Medical University, College Town, Gui’an New District, Guiyang, 550025, People’s Republic of China, Tel/fax +86 851 882 59268, Email guoguojsc@163.comBackground: Cryptococcus neoformans is a common human fungal pathogen in immunocompromised people, as well as a prevalent cause of meningitis in HIV-infected individuals. With the emergence of clinical fungal resistance and the shortage of antifungal drugs, it is urgent to discover novel antifungal agents. AMP-17, a novel antimicrobial peptide from Musca domestica, has antifungal activity against C. neoformans. However, its antifungal and anti-biofilm activities remain unclear. Thus, this study aimed to evaluate the antifungal activity of AMP-17 against planktonic cells and biofilms of C. neoformans.Methods: The minimum inhibitory concentration (MIC), the biofilm inhibitory and eradicating concentration (BIC and BEC) were determined by the broth microdilution assay or the 2, 3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay, respectively. The inhibitory and killing activities of AMP-17 against C. neoformans were investigated through the time-inhibition/killing kine

Published
2022

9. Flavonoid Glycosides of Polygonum capitatum Protect against Inflammation Associated with Helicobacter pylori Infection

Author

Jian Huang, Zhang Ran, Shu Zhang, Sun Chaoqin, Fei Mo, and Luo Zhaoxun

Subjects
Male, Flavonoid, lcsh:Medicine, Pharmacology, Helicobacter Infections, Interferon-gamma, Mice, Clarithromycin, Gastrins, medicine, Animals, Glycosides, lcsh:Science, Omeprazole, Gastrin, Flavonoids, Inflammation, chemistry.chemical_classification, Multidisciplinary, Helicobacter pylori, biology, lcsh:R, Glycoside, Amoxicillin, biology.organism_classification, Anti-Bacterial Agents, Mice, Inbred C57BL, chemistry, Gastritis, Immunology, Female, lcsh:Q, Polygonum, medicine.symptom, Phytotherapy, Research Article, medicine.drug
Abstract

The antibacterial and anti-inflammatory activities, and protective effects of extracts (flavonoid glycosides) of Polygonum capitatum were investigated to detect the evidence for the utilization of the herb in the clinical therapy of gastritis caused by H. pylori. A mouse gastritis model was established using H. pylori. According to treating methods, model mice were random assigned into a model group (MG group), a triple antibiotics group (TG group, clarithromycin, omeprazole and amoxicillin), low/middle/high concentrations of flavonoid glycosides groups (LF, MF and HF groups) and low/middle/high concentrations of flavonoid glycosides and amoxicillin groups (LFA, MFA and HFA groups). A group with pathogen-free mice was regarded as a control group (CG group). The eradicate rates of H. pylori were 100%, 93%, 89% in TG, MFA and HF groups. The serum levels of IFN-gamma and gastrin were higher in a MG group than those from all other groups (P < 0.05). The serum levels of IFN-gamma and gastrin were reduced significantly in LF, MF and HF groups (P < 0.05) while little changes were observed in LFA, MFA and HFA groups. In contrast, the serum levels of IL-4 were lower and higher in MG and CG groups compared with other groups (P

Published
2015

11. [Identification of the target site of antimicrobial peptide AMP-17 against Candida albicans ].

Author

Yang L, Tian Z, Zhou L, Sun C, Huang M, Tian C, Peng J, and Guo G

Subjects
Humans, Proteomics, Peptides pharmacology, Transcription Factors metabolism, Antifungal Agents pharmacology, Candida albicans, Antimicrobial Peptides
Abstract

Candida albicans is one of the major causes of invasive fungal infections and a serious opportunistic pathogen in immunocompromised individuals. The antimicrobial peptide AMP-17 has prominent anti- Candida activity, and proteomic analysis revealed significant differences in the expression of cell wall ( XOG1 ) and oxidative stress ( SRR1 ) genes upon the action of AMP-17 on C . albicans , suggesting that AMP-17 may exert anti- C . albicans effects by affecting the expression of XOG1 and SRR1 genes. To further investigate whether XOG1 and SRR1 genes were the targets of AMP-17, C . albicans xog1Δ/Δ and srr1Δ/Δ mutants were constructed using the clustered regulatory interspaced short palindromic repeats-associated protein 9 (CRISPR/Cas9) system. Phenotypic observations revealed that deletion of two genes had no significant effect on C . albicans growth and biofilm formation, whereas XOG1 gene deletion affected in vitro stress response and mycelium formation of C . albicans . Drug sensitivity assay showed that the MIC 80 values of AMP-17 against xog1Δ/Δ and srr1Δ/Δ mutants increased from 8 μg/mL (for the wild type C . albicans SC5314) to 16 μg/mL, while the MIC 80 values against srr1Δ/Δ : : srr1 revertants decreased to the level of the wild type SC5314. In addition, the ability of AMP-17 to inhibit biofilm formation of both deletion strains was significantly reduced compared to that of wild type SC5314, indicating that the susceptibility of the deletion mutants to AMP-17 was reduced in both the yeast state and during biofilm formation. These results suggest that XOG1 and SRR1 genes are likely two of the potential targets for AMP-17 to exert anti -C . albicans effects, which may facilitate further exploration of the antibacterial mechanism of novel peptide antifungal drugs.

Published
2023
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