Search

Your search keyword '"Sun, Runrun"' showing total 29 results
Start Over Author "Sun, Runrun"
29 results on '"Sun, Runrun"'

3. Genome-Wide Association Study of Lint Percentage in Gossypium hirsutum L. Races

Author

Wang, Yuanyuan, primary, Guo, Xinlei, additional, Cai, Xiaoyan, additional, Xu, Yanchao, additional, Sun, Runrun, additional, Umer, Muhammad Jawad, additional, Wang, Kunbo, additional, Qin, Tengfei, additional, Hou, Yuqing, additional, Wang, Yuhong, additional, Zhang, Pan, additional, Wang, Zihan, additional, Liu, Fang, additional, Wang, Qinglian, additional, and Zhou, Zhongli, additional

Published
2023
Full Text
View/download PDF

15. Verticillium dahliae VdTHI20, Involved in Pyrimidine Biosynthesis, Is Required for DNA Repair Functions and Pathogenicity

Author

Qin, Tengfei, primary, Hao, Wei, additional, Sun, Runrun, additional, Li, Yuqing, additional, Wang, Yuanyuan, additional, Wei, Chunyan, additional, Dong, Tao, additional, Wu, Bingjie, additional, Dong, Na, additional, Wang, Weipeng, additional, Sun, Jialiang, additional, Yang, Qiuyue, additional, Zhang, Yaxin, additional, Yang, Song, additional, and Wang, Qinglian, additional

Published
2020
Full Text
View/download PDF

28. Overexpression of miRNA in Cotton via Agrobacterium-Mediated Transformation.

Author

Wang M, Sun R, Wang Q, and Zhang B

Subjects
Gene Expression Regulation, Plant, RNA Interference, Agrobacterium physiology, Gene Expression, Gossypium genetics, MicroRNAs genetics, Transformation, Genetic
Abstract

microRNAs (miRNAs) are an extensive class of newly identified endogenous small regulatory molecules. Many studies show that miRNAs play a critical role in almost all biological and metabolic progress through targeting protein-coding genes for mRNA cleavage or translation inhibition. Many miRNAs are also identified from cotton using computational and/or experimental approaches, including the next-generation deep sequencing technology. However, their functions are unclear. In this chapter, we describe a detailed method for overexpressing miRNA, miR156 as an example, in cotton using Agrobacterium-mediated genetic transformation. This provides an approach to investigate the function and regulatory mechanism of miRNAs in cotton.

Published
2019
Full Text
View/download PDF

29. Pollen Tube Pathway-Mediated Cotton Transformation.

Author

Wang M, Sun R, Zhang B, and Wang Q

Subjects
Genotype, Microinjections, Plants, Genetically Modified, Polymerase Chain Reaction, Transgenes, Gossypium genetics, Gossypium metabolism, Pollen Tube metabolism, Signal Transduction, Transformation, Genetic
Abstract

Although many gene transfer methods have been employed for successfully obtaining transgenic cotton, the major constraint in cotton improvement is the limitation of genotype because the majority of transgenic methods require plant regeneration from a single transformed cell which is limited by cotton tissue culture. Comparing with other plant species, it is difficult to induce plant regeneration from cotton; currently, only a limited number of cotton cultivars can be cultured for obtaining regenerated plants. Thus, developing a simple and genotype-independent genetic transformation method is particularly interested for cotton. In this chapter, we present a simple, cost-efficient, and genotype-independent cotton transformation method - pollen tube pathway-mediated transformation. This method uses pollen tube pathway to deliver transgene into cotton embryo sacs and then insert foreign genes into cotton genome. There are three major steps for pollen tube pathway-mediated genetic transformation, which include injection of foreign genes into pollen tube, integration of foreign genes into plant genome, and selection of transgenic plants.

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results