Search

Your search keyword '"Sun, Xianbao"' showing total 211 results
Start Over Author "Sun, Xianbao"
211 results on '"Sun, Xianbao"'

32. CBT‐Cys click reaction for optical bioimaging in vivo.

Author

Hu, Xinyi, Tang, Runqun, Bai, Lin, Liu, Songqin, Liang, Gaolin, and Sun, Xianbao

Subjects
LINEAR polymers, OPTICAL images, RATE coefficients (Chemistry), CONDENSATION reactions, OLIGOMERS, DNA nanotechnology
Abstract

Derived from the D‐luciferin regeneration pathway in firefly body, the click condensation reaction between 2‐cyanobenzothiazole (CBT) and D‐cysteine (Cys) (CBT‐Cys click reaction) possesses unique advantages, including superior biocompatibility, high second order reaction rate, and metal‐free mild conditions, emerging as a powerful bioorthogonal tool for a variety of chemical biological applications. Moreover, owing to its programmable controllability (e.g., pH, reduction, or enzyme), CBT‐Cys click reaction is exploited to fabricate stimuli‐activatable imaging probes with self‐assembling behaviors in physiological context. At stimuli‐rich pathological lesions of interest, these probes undergo CBT‐Cys click reaction to form cyclic dimers/oligomers or linear polymers, and further self‐assemble into nanostructures. The in situ formed nanostructures promote the selective accumulation and retention of imaging agent cargos at pathological lesions, thus enabling precise and enhanced in vivo imaging of diseases (especially tumors). To address the significance and recent breakthroughs of smart CBT‐Cys probes for enhanced optical imaging of tumors/other diseases, we herein propose this mini‐review, in which advances (particularly in recent 5 years) and potential challenges (or chances) in this field are emphasized. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

42. Caspase-3-Triggered Intracellular Gadolinium Nanoparticle Formation for T1-Weighted Magnetic Resonance Imaging of Apoptosis In Vivo

Author

Xu, Hai-Dong, Cheng, Xiaotong, Sun, Xianbao, Chen, Peiyao, Zhan, Wenjun, Liu, Xiaoyang, Wang, Xinliang, Hu, Bing, and Liang, Gaolin

Abstract

Apoptosis, with a hallmark of upregulated protease Caspase-3, has been frequently imaged with various probes to reveal the therapeutic efficiencies of different drugs. However, activatable molecular probes with programmable self-assembling behaviors that enable enhanced T1-weighted magnetic resonance imaging (MRI) of apoptosis remain scarce. Herein, taking advantage of a CBT-Cys click reaction, we rationally designed a Caspase-3-activatable self-assembling probe Ac-Asp-Glu-Val-Asp-Cys(StBu)-Lys(DOTA(Gd))-CBT (DEVDCS-Gd-CBT) for apoptosis imaging in vivo. After Caspase-3 cleavage in apoptotic cells, DEVDCS-Gd-CBTunderwent CBT-Cys click reaction to form a cyclic dimer, which self-assembled into Gd nanoparticles. With this probe, enhanced T1-weighted MR images of apoptosis were achieved at low magnetic fields in vitro, in cis-dichlorodiamineplatinum-induced apoptotic cells and in tail-amputation-simulated apoptotic zebrafish. We anticipate that the smart probe DEVDCS-Gd-CBTcould be applied for T1-weighted MRI of apoptosis-related diseases in the clinic in the future.

Published
2023
Full Text
View/download PDF

49. Isolation of Epigallocatechin Gallate from Green Tea and its Effects on Probiotics and Pathogenic Bacteria

Author

Zhang Zuoyong, Zhu Yongsheng, He Shudong, Sun Han-ju, Yu Ming, Sun Xianbao, and Tu Lijun

Subjects
0301 basic medicine, 030109 nutrition & dietetics, Nutrition and Dietetics, food and beverages, Medicine (miscellaneous), Pathogenic bacteria, Epigallocatechin gallate, Biology, Isolation (microbiology), medicine.disease_cause, Green tea, complex mixtures, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, chemistry, medicine, heterocyclic compounds, sense organs
Abstract

The aim of this study was to investigate epigallocatechin gallate (EGCG) prebiotics activities systematically which was reported as a bioactive substance. Therefore, EGCG was separated by water extraction, resin purification and prep-HPLC. Then the production of EGCG was confirmed by HPLC and mass spectrometry (MS) analysis and its purify was 97.23%. EGCG extractive and green tea extract (GTE) were further incubated with Bifidobacterium infantis, B. adolescentis, B. bifidum and Lactobacillus acidophilus to study its effect on microbial populations and medium pH. Finally, Escherichia coli, Salmonella, Staphylococcus aureus and Candida albicans were employed as pathogenic bacteria to explore the antimicrobial activity of EGCG and GTE. The results demonstrated that EGCG extractive could be beneficial for the proliferation of Bifidobacterium and L. acidophilus and also inhibit some pathogenic bacteria. In conclusion, both EGCG extractive and GTE had prebiotics activities and the effects of EGCG extractive were superior to those of GTE.

Published
2017

Catalog

Books, media, physical & digital resources
See catalog results